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A method of removing retardance induced by scattering of collagenous fiber bundles 被引量:1
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作者 Wanrong Gao Siyu Liu 《Journal of Innovative Optical Health Sciences》 SCIE EI CAS 2021年第1期39-45,共7页
In this work,we report a method of removing scattering induced retardance in polarization sensitive fnll field optical coherence tomography(PS-FFOCT).First,the Mueller matrix that describes its operation is derived.Th... In this work,we report a method of removing scattering induced retardance in polarization sensitive fnll field optical coherence tomography(PS-FFOCT).First,the Mueller matrix that describes its operation is derived.The thickness invariant retardance induced by the scattering of collagenous fiber bundles is then used to find the accurate values of the birefringence of the layers that consist collagenous fibers.Finally,the initial en face birefringent images of in vitro beef tendon samples are presented to demonstrate the capability of our method. 展开更多
关键词 Mueller matrix polarimeter birefringent structures collagenous fibers
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APPLICATION OF THE COLLAGENOUS FIBER FROM THE SOLID WASTES OF LEATHER FOR PAPERMAKING 被引量:1
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作者 ZhijieWang JianWang +1 位作者 ZhangChuanbo HouXiaodong 《天津科技大学学报》 CAS 2004年第A01期312-315,共4页
The capability of pulping for the solid waste of leather was investigated. The properties of paper that made up of collagenous fiber and plant fiber were also analyzed. The result showed that by proper treatment, soli... The capability of pulping for the solid waste of leather was investigated. The properties of paper that made up of collagenous fiber and plant fiber were also analyzed. The result showed that by proper treatment, solid waste of leather could be made into collagen fiber for papermaking. The physical strength of paper can be enhanced by appending collagenous fiber in a proper propriety. 展开更多
关键词 成胶纤维 固体废物 造纸工业 植物纤维 酸性水解
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Estimation of cancer cell migration in biomimetic random/oriented collagen fiber microenvironments
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作者 姚静如 李国强 +8 位作者 姚喜耀 周连杰 叶志凯 刘艳平 郑栋天 唐婷 宋克纳 陈果 刘雳宇 《Chinese Physics B》 SCIE EI CAS CSCD 2024年第5期749-756,共8页
Increasing data indicate that cancer cell migration is regulated by extracellular matrixes and their surrounding biochemical microenvironment,playing a crucial role in pathological processes such as tumor invasion and... Increasing data indicate that cancer cell migration is regulated by extracellular matrixes and their surrounding biochemical microenvironment,playing a crucial role in pathological processes such as tumor invasion and metastasis.However,conventional two-dimensional cell culture and animal models have limitations in studying the influence of tumor microenvironment on cancer cell migration.Fortunately,the further development of microfluidic technology has provided solutions for the study of such questions.We utilize microfluidic chip to build a random collagen fiber microenvironment(RFM)model and an oriented collagen fiber microenvironment(OFM)model that resemble early stage and late stage breast cancer microenvironments,respectively.By combining cell culture,biochemical concentration gradient construction,and microscopic imaging techniques,we investigate the impact of different collagen fiber biochemical microenvironments on the migration of breast cancer MDA-MB-231-RFP cells.The results show that MDA-MB-231-RFP cells migrate further in the OFM model compared to the RFM model,with significant differences observed.Furthermore,we establish concentration gradients of the anticancer drug paclitaxel in both the RFM and OFM models and find that paclitaxel significantly inhibits the migration of MDA-MB-231-RFP cells in the RFM model,with stronger inhibition on the high concentration side compared to the low concentration side.However,the inhibitory effect of paclitaxel on the migration of MDA-MB-231-RFP cells in the OFM model is weak.These findings suggest that the oriented collagen fiber microenvironment resembling the late-stage tumor microenvironment is more favorable for cancer cell migration and that the effectiveness of anticancer drugs is diminished.The RFM and OFM models constructed in this study not only provide a platform for studying the mechanism of cancer development,but also serve as a tool for the initial measurement of drug screening. 展开更多
关键词 microfluidic chip random collagen fiber microenvironment(RFM) oriented collagen fiber microenvironment(OFM) cancer cell migration
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Anti-abrasion collagen fiber-based membrane functionalized by UiO-66-NH_(2)with ultra-high efficiency and stability for oil-in-water emulsions separation
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作者 Xiaoxia Ye Rixin Huang +3 位作者 Zhihong Zheng Juan Liu Jie Chen Yuancai Lv 《Chinese Journal of Chemical Engineering》 SCIE EI CAS CSCD 2024年第2期285-297,共13页
Membrane separation strategies offer promising platform for the emulsion separation.However,the low mechanical strength of membrane separation layers and the trade-off between separation flux and efficiency present si... Membrane separation strategies offer promising platform for the emulsion separation.However,the low mechanical strength of membrane separation layers and the trade-off between separation flux and efficiency present significant challenges.In this study,we report a CFM@UiO-66-NH_(2)membrane with high separation flux,efficiency and stability,through utilizing a robust anti-abrasion collagen fiber membrane(CFM)as the multifunctional support and UiO-66-NH_(2)by an in-situ growth as the separation layer.The high mechanical strength of the CFM compensated for the weakness of the separation layer,while the charge-breaking effect of UiO-66-NH_(2),along with the size sieving of its constituent separating layers and the capillary effect of the collagen fibers,contributed to the potential for efficient separation.Additionally,the CFM@UiO-66-NH_(2)membrane exhibited superhydrophilic properties,making it suitable for separating oil-in-water microemulsions and nanoemulsions stabilized by anionic surfactants.The membrane demonstrated remarkable separation efficiencies of up to 99.960%and a separation flux of370.05 L·m^(-2)·h^(-1).Moreover,it exhibits stability,durability,and abrasion resistance,maintaining excellent separation performance even when exposed to strong acids and alkalis without any damage to its structure and performance.After six cycles of reuse,it achieved a separation flux of 417.97 L·m^(-2)·h^(-1)and a separation efficiency of 99.747%.Furthermore,after undergoing 500 cycles of strong abrasion,the separation flux remained at 124.39 L·m^(-2)·h^(-1),with a separation efficiency of 99.992%.These properties make it suitable for the long-term use in harsh operating environments.We attribute these properties to the electrostatic effect resulting from the amino group on UiO-66-NH_(2)and its in-situ growth on the CFM,which forms a size-screening separation layer.Our work highlights the potential of the CFM@UiO-66-NH_(2)membrane as an environmentally friendly size-screening material for the efficient emulsion wastewater separation. 展开更多
关键词 Collagen fibers Metal-organic frameworks Oil-in-water emulsion separation Size sieving
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Dietary protein levels changed the hardness of muscle by acting on muscle fiber growth and the metabolism of collagen in sub-adult grass carp(Ctenopharyngodon idella) 被引量:1
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作者 Min Dong Lu Zhang +8 位作者 Pei Wu Lin Feng Weidan Jiang Yang Liu Shengyao Kuang Shuwei Li Haifeng Mi Ling Tang Xiaoqiu Zhou 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2023年第2期729-744,共16页
Background:Nutrient regulation has been proven to be an effective way to improve the flesh quality in fish.As a necessary nutrient for fish growth,protein accounts for the highest proportion in the fish diet and is ex... Background:Nutrient regulation has been proven to be an effective way to improve the flesh quality in fish.As a necessary nutrient for fish growth,protein accounts for the highest proportion in the fish diet and is expensive.Although our team found that the effect of protein on the muscle hardness of grass carp was probably related to an increased collagen content,the mechanism for this effect has not been deeply explored.Moreover,few studies have explored the protein requirements of sub-adult grass crap(Ctenopharyngodon idella).Therefore,the effects of different dietary protein levels on the growth performance,nutritional value,muscle hardness,muscle fiber growth,collagen metabolism and related molecule expression in grass carp were investigated.Methods:A total of 450 healthy grass carp(721.16±1.98 g)were selected and assigned randomly to six experimen-tal groups with three replicates each(n=25/replicate),and were fed six diets with 15.91%,19.39%,22.10%,25.59%,28.53%and 31.42%protein for 60 d.Results:Appropriate levels of dietary protein increased the feed intake,percentage weight gain,specific growth rate,body composition,unsaturated fatty acid content in muscle,partial free amino acid content in muscle,and muscle hardness of grass carp.These protein levels also increased the muscle fiber density,the frequency of new muscle fibers,the contents of collagen and IGF-1,and the enzyme activities of prolyl 4-hydroxylases and lysyloxidase,and decreased the activity of matrix metalloproteinase-2.At the molecular level,the optimal dietary protein increased col-lagen type Iα1(Colα1),Colα2,PI3K,Akt,S6K1,La ribonucleoprotein domain family member 6a(LARP6a),TGF-β1,Smad2,Smad4,Smad3,tissue inhibitor of metalloproteinase-2,MyoD,Myf5,MyoG and MyHC relative mRNA levels.The levels of the myostatin-1 and myostatin-2 genes were downregulated,and the protein expression levels of p-Smad2,Smad2,Smad4,p-Akt,Akt,LARP6 and Smad3 were increased.Conclusions:The appropriate levels of dietary protein promoted the growth of sub-adult grass carp and improved muscle hardness by promoting the growth of muscle fibers,improving collagen synthesis and depressing collagen degradation.In addition,the dietary protein requirements of sub-adult grass carp were 26.21%and 24.85%according to the quadratic regression analysis of growth performance(SGR)and the muscle hardness(collagen content),respectively. 展开更多
关键词 Collagen degradation Collagen synthesis Grass carp Muscle fibers Muscle hardness PROTEIN
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Three-dimensional remodeling of collagen fibers within cervical tissues in pregnancy
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作者 Lingxi Zhou Rushan Jiang +7 位作者 Jia Meng Shuhao Qian Shenyi Jiang Chuncheng Wang Chen Yang Zhihua Ding Zheyue Shu Zhiyi Liu 《Journal of Innovative Optical Health Sciences》 SCIE EI CSCD 2023年第4期66-78,共13页
The cervix is a collagen-rich connective tissue that must remain closed during pregnancy while undergoing progressive remodeling in preparation for delivery,which begins before the onset of the preterm labor process.T... The cervix is a collagen-rich connective tissue that must remain closed during pregnancy while undergoing progressive remodeling in preparation for delivery,which begins before the onset of the preterm labor process.Therefore,it is important to resolve the changes of collagen flbers during cervical remodeling for the prevention of preterm labor.Herein,we assessed the spatial organization of collagen flbers in a three-dimensional(3D)context within cervical tissues of mice on day 3,9,12,15 and 18 of gestation.We found that the 3D directional variance,a novel metric of alignment,was higher on day 9 than that on day 3 and then gradually decreased from day 9 to day 18.Compared with two-dimensional(2D)approach,a higher sensitivity was achieved from 3D analysis,highlighting the importance of truly 3D quantification.Moreover,the depthdependent variation of 3D directional variance was investigated.By combining multiple 3D directional variance-derived metrics,a high level of classification accuracy was acquired in distinguishing different periods of pregnancy.These results demonstrate that 3D directional variance is sensitive to remodeling of collagen fibers within cervical tissues,shedding new light on highly-sensitive,early detection of preterm birth(PTB). 展开更多
关键词 Preterm birth cervical tissue directional variance collagen fiber 3D analysis
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牦牛肺脏内纤维和纤维蛋白分布与表达的增龄性变化研究
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作者 李慧娟 王彪 +2 位作者 崔燕 张倩 何俊峰 《核农学报》 CAS CSCD 北大核心 2024年第12期2339-2346,共8页
为探究牦牛肺内纤维结构及其组成蛋白的增龄性变化,本研究采用Verhoeff’s Van Gieson(EVG)染色和网状纤维染色观察胶原纤维、弹性纤维及网状纤维在初生、幼年和成年牦牛肺组织中的分布情况;利用免疫组化、免疫荧光和蛋白免疫印迹对不... 为探究牦牛肺内纤维结构及其组成蛋白的增龄性变化,本研究采用Verhoeff’s Van Gieson(EVG)染色和网状纤维染色观察胶原纤维、弹性纤维及网状纤维在初生、幼年和成年牦牛肺组织中的分布情况;利用免疫组化、免疫荧光和蛋白免疫印迹对不同年龄组牦牛肺组织中Ⅰ、Ⅲ、Ⅳ型胶原蛋白和弹性蛋白的分布特征及蛋白表达水平进行研究。结果显示,胶原纤维、网状纤维及三种胶原蛋白的分布位置基本一致,主要分布于牦牛肺脏支气管及血管外膜、软骨片、气管腺,支气管上皮细胞基膜、平滑肌层及肺泡隔也有较少量分布;而弹性蛋白及弹性纤维大量分布于支气管上皮细胞基膜、肺血管及肺泡隔中。四种蛋白的表达趋势基本一致,均在幼年组表达最高,初生组表达最低,且各年龄组间均差异显著(P<0.05)。结果表明,牦牛肺内弹性纤维、胶原纤维、网状纤维分布丰富,可使牦牛肺具有更好的收缩和舒张能力,有利于其对高寒缺氧环境的适应;Ⅰ、Ⅲ、Ⅳ型胶原蛋白和弹性蛋白表达量与年龄呈相关性,在幼年段表达最高,说明牦牛肺脏低氧适应性结构的形成在幼年段最为显著。本研究为进一步探究牦牛肺脏低氧适应结构提供了基础资料。 展开更多
关键词 牦牛 低氧 肺脏内纤维 胶原蛋白 弹性蛋白
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胶原纤维基吸附材料的研究进展
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作者 程佳丽 庞楠炯 +2 位作者 刘娴 廖学品 石碧 《皮革科学与工程》 CAS 北大核心 2024年第5期40-49,共10页
吸附在化学化工等领域有着至关重要的作用,开发绿色、低成本的高效吸附材料是未来吸附分离的重要发展方向。胶原纤维(CFs)是一种来源于动物皮的天然生物质资源,其具有独特的三维多层级结构,同时含有丰富的活性官能团。因此,利用CFs制备... 吸附在化学化工等领域有着至关重要的作用,开发绿色、低成本的高效吸附材料是未来吸附分离的重要发展方向。胶原纤维(CFs)是一种来源于动物皮的天然生物质资源,其具有独特的三维多层级结构,同时含有丰富的活性官能团。因此,利用CFs制备高性能吸附材料具有较高的研究价值和应用潜力。论文主要介绍了近年来CFs固化单宁、负载金属离子和接枝功能基团等CFs基吸附材料的制备方法,讨论了吸附材料在放射性核素离子、有毒重金属离子、无机阴离子、有机污染物的去除,以及天然产物的吸附分离和细胞固定等方面的应用,并对CFs基吸附材料未来的发展方向进行了展望。 展开更多
关键词 胶原纤维 固化单宁 负载金属离子 接枝 吸附材料
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胶原纤维基氮掺杂碳负载Ru催化二苯醚氢解
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作者 张家铭 周倩 +3 位作者 段景峰 孙宇航 陈奕霏 肖霄 《皮革科学与工程》 CAS 北大核心 2024年第2期22-29,共8页
利用胶原纤维富含N元素的特点,以胶原纤维为基底,借鉴胶原纤维固化单宁法结合高温碳化,制备了基于胶原纤维的氮掺杂碳负载Ru催化剂,用于木质素催化氢解。红外光谱和X射线光电子能谱分析表明Ru通过与单宁酸的络合作用负载在胶原纤维上,... 利用胶原纤维富含N元素的特点,以胶原纤维为基底,借鉴胶原纤维固化单宁法结合高温碳化,制备了基于胶原纤维的氮掺杂碳负载Ru催化剂,用于木质素催化氢解。红外光谱和X射线光电子能谱分析表明Ru通过与单宁酸的络合作用负载在胶原纤维上,经碳化后形成胶原纤维基碳负载Ru催化剂(Ru/TNC)。扫描电镜、透射电镜和电感耦合等离子体发射光谱分析表明,Ru/TNC具有较低的Ru负载量(1.14%)和丰富孔隙结构,Ru以较小的纳米颗粒形式均匀负载在碳载体表面。二苯醚氢解结果表明,相比传统浸渍法制备氮掺杂碳负载Ru,Ru/TNC具有更好的性能,其催化二苯醚氢解转化率在280℃时达100%,且产物均为小分子单体,包括苯(22.2%)、环己烷(27.8%)、环己醇(35.7%)和环己酮(14.3%)。 展开更多
关键词 胶原纤维 生物质 木质素 催化 氢解
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超声波辅助酶法提取猪皮胶原蛋白的工艺优化及胶原纤维的湿法纺丝制备
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作者 徐敏杰 唐树淇 +3 位作者 胡敏 蔡光明 张如全 岳程飞 《纺织工程学报》 2024年第5期40-50,共11页
胶原纤维材料是一种重要的生物医用纺织品,广泛用于各类外科手术。为了提高猪皮源胶原蛋白的提取率,设定酶浓度、超声强度、超声时间为影响因素,胶原提取率为响应值,采用超声波辅助酶法,通过单因素实验和Box-Behnken响应面法探究最佳提... 胶原纤维材料是一种重要的生物医用纺织品,广泛用于各类外科手术。为了提高猪皮源胶原蛋白的提取率,设定酶浓度、超声强度、超声时间为影响因素,胶原提取率为响应值,采用超声波辅助酶法,通过单因素实验和Box-Behnken响应面法探究最佳提取条件,并采用湿法纺丝技术制备猪皮源胶原纤维。结果表明:影响胶原提取率的显著性顺序为:酶浓度>超声时间>超声强度,最佳提取工艺的酶浓度为93 U/g、超声强度为162 W、超声时间为58 min,所制备的胶原提取率56.21%,与响应面回归方程预测值相近,说明该回归模型能够较好地优化提取工艺条件。同时,紫外光谱、红外光谱和X射线衍射分析表明猪皮源胶原的类型为Ⅰ型,具有完整的三螺旋结构;纤维形貌分析表明猪皮源胶原具有良好的成纤性;力学性能分析表明纺丝液为4.0%浓度的胶原纤维具有最佳的力学性能。 展开更多
关键词 猪皮胶原蛋白 超声波辅助酶法 响应面法 胶原纤维 湿法纺丝
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废弃胶原纤维固化单宁吸附材料原位Fe_(2)O_(3)微波催化裂解减容研究
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作者 李林倚 肖霄 +1 位作者 廖学品 石碧 《皮革科学与工程》 CAS 北大核心 2024年第6期9-15,共7页
胶原纤维基吸附材料在核素吸附中表现出优异的性能,但同时产生放射性固废的处置问题。针对这一问题,利用原位Fe_(2)O_(3)对胶原纤维固化单宁(CFT)材料进行催化裂解,以实现废弃CFT的有效减容。通过热重分析技术对其热解过程进行研究,结... 胶原纤维基吸附材料在核素吸附中表现出优异的性能,但同时产生放射性固废的处置问题。针对这一问题,利用原位Fe_(2)O_(3)对胶原纤维固化单宁(CFT)材料进行催化裂解,以实现废弃CFT的有效减容。通过热重分析技术对其热解过程进行研究,结果表明通过将Fe^(3+)负载至CFT(Fe-CFT)再进行裂解,能够使裂解温度降至约450℃,比CFT直接裂解温度降低了约150℃,残渣率降至9.5%。进一步以纳米Fe_(2)O_(3)为传热介质,在微波条件下对Fe-CFT进行裂解,Fe-CFT残渣率降至2.1%。利用傅里叶变换红外光谱仪、X射线衍射仪和元素分析仪对残渣进行表征,结果表明反应过程中Fe^(3+)原位生成的Fe_(2)O_(3)有效促进CFT的氧化裂解。综上,通过原位Fe_(2)O_(3)有效降低CFT的裂解温度,裂解后残渣率低,实现了废弃CFT的安全高效减容。 展开更多
关键词 胶原纤维 单宁 吸附 原位Fe_(2)O_(3) 催化裂解 减容 微波裂解
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高比例再生胶原蛋白纤维混纺纱的工艺优化 被引量:1
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作者 李洋 张书峰 +1 位作者 孙启畅 宋静 《棉纺织技术》 CAS 2024年第3期67-71,共5页
探讨一款高比例再生胶原蛋白纤维混纺纱的生产要点及工艺优化措施。介绍了再生胶原蛋白纤维性能特点以及优势,根据其特有的强力低、不耐高温、回潮大等性能,重点对纺纱工艺流程进行了调整与优化,对清梳工序进行了设备流程的改造;梳棉加... 探讨一款高比例再生胶原蛋白纤维混纺纱的生产要点及工艺优化措施。介绍了再生胶原蛋白纤维性能特点以及优势,根据其特有的强力低、不耐高温、回潮大等性能,重点对纺纱工艺流程进行了调整与优化,对清梳工序进行了设备流程的改造;梳棉加装温度检测感应器,以避免温度过高对纤维造成损伤等;加强了柔性梳理的研究与工艺优化,在保证充分梳理的前提下尽量降低短纤维的产生;在以牵伸为主的工序,从胶辊、涂料方面减少纤维缠绕,最终成功开发出赛络集聚纺再生胶原蛋白纤维/莫代尔纤维70/3014.8 tex纱,进一步拓展了再生胶原蛋白纤维纱的应用领域。 展开更多
关键词 再生胶原蛋白纤维 柔性梳理与牵伸 温感器 棉结 强力
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脆肉鲩器官组织胶原纤维染色观察
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作者 张玮岚 杜瑞雪 +1 位作者 叶元土 肖旭全 《水产学杂志》 CAS 2024年第1期16-21,38,共7页
为研究饲喂蚕豆的脆肉鲩(Ctenopharyngodon idella)与普通草鱼不同器官组织中胶原纤维分布的特征,本试验对体质量7.0 kg的脆肉鲩和体质量4.5 kg的普通草鱼背部肌肉、腹部肌肉、心脏、肝胰脏、脾脏、前肠、中肠及后肠组织制作Masson染色... 为研究饲喂蚕豆的脆肉鲩(Ctenopharyngodon idella)与普通草鱼不同器官组织中胶原纤维分布的特征,本试验对体质量7.0 kg的脆肉鲩和体质量4.5 kg的普通草鱼背部肌肉、腹部肌肉、心脏、肝胰脏、脾脏、前肠、中肠及后肠组织制作Masson染色切片,分析胶原纤维数量及分布。结果显示:器官组织中胶原纤维被染成蓝色,肌纤维被染成红色。脆肉鲩背肌、腹肌的胶原纤维染色更深、分布面积更大;背肌横切面、腹肌横切面的胶原纤维容积分数分别是普通草鱼的1.27倍和2.09倍,显示出脆肉鲩肌肉组织中胶原纤维含量更丰富;脆肉鲩肝胰脏、脾脏和心脏组织中,胶原纤维主要分布在肝胰脏门管区中的动脉、静脉管壁间隙、脾脏脾索内及心脏结缔组织中。对比发现,普通草鱼和脆肉鲩肠道组织平滑肌的大量胶原纤维分布在平滑肌细胞间,且脆肉鲩前、中、后肠的胶原纤维容积分数分别是普通草鱼的2.16倍、1.68倍和2.66倍。与肌肉、肝胰脏、脾脏和心脏相比,肠道的胶原纤维含量显著增多。器官组织的纵切和横切片显示,肌肉(骨骼肌细胞)、肠道(平滑肌细胞)和心脏(心肌细胞)的肌细胞类型并未发生改变,改变还是胶原纤维数量与分布、结缔组织的结构等。 展开更多
关键词 脆肉鲩 器官组织 胶原纤维 MASSON染色
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槟榔碱黏膜下注射法构建SD大鼠口腔黏膜下纤维化模型
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作者 郭振兴 王悦 +2 位作者 许昊清 王舒妍 焦凯 《口腔生物医学》 2024年第1期21-25,37,共6页
目的:通过黏膜下注射槟榔碱溶液构建SD大鼠口腔黏膜下纤维化(OSF)模型。方法:将20只SD大鼠随机分为实验组和对照组。实验组大鼠采用口腔颊黏膜下注射槟榔碱溶液进行造模,对照组大鼠则注射生理盐水。处理10周后,观察大鼠口腔黏膜颜色及... 目的:通过黏膜下注射槟榔碱溶液构建SD大鼠口腔黏膜下纤维化(OSF)模型。方法:将20只SD大鼠随机分为实验组和对照组。实验组大鼠采用口腔颊黏膜下注射槟榔碱溶液进行造模,对照组大鼠则注射生理盐水。处理10周后,观察大鼠口腔黏膜颜色及体质量变化,测量被动开口度;苏木素-伊红(HE)染色、Masson染色观察黏膜组织病理学变化;免疫组化染色和实时荧光定量PCR检测黏膜组织内平滑肌肌动蛋白(α-SMA)、血小板-内皮细胞黏附分子(PECAM-1/CD31)蛋白及基因表达水平。结果:实验组大鼠颊黏膜出现白色病变,张口度减小(P<0.001);大鼠颊黏膜上皮萎缩,固有层胶原纤维沉积;大鼠黏膜组织内α-SMA蛋白和基因表达显著增加(P<0.001),CD31蛋白和基因表达显著减少(P<0.001)。结论:槟榔碱黏膜下注射法可构建出具有OSF典型症状、病理变化符合OSF的大鼠模型。 展开更多
关键词 口腔黏膜下纤维化 槟榔碱 动物模型 胶原纤维沉积
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单宁酸-纳米协同改性胶原纤维多孔材料的制备及其油水分离性能
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作者 石佳博 张宇轩 +1 位作者 陈雪峰 谭蕉君 《化工进展》 EI CAS CSCD 北大核心 2024年第8期4624-4629,共6页
以胶原纤维为代表的生物质基多孔材料因其具有可再生、储量丰富等特点且保留胶原纤维独特的多尺度孔隙结构以及具有优异的分离能力、可重复使用和可生物降解等优势,近年在含油废水处理等领域备受关注。针对胶原纤维多孔材料存在着油水... 以胶原纤维为代表的生物质基多孔材料因其具有可再生、储量丰富等特点且保留胶原纤维独特的多尺度孔隙结构以及具有优异的分离能力、可重复使用和可生物降解等优势,近年在含油废水处理等领域备受关注。针对胶原纤维多孔材料存在着油水分离效率低等问题,本文利用单宁酸与金属离子(铝和锆)掺杂锂藻土纳米片的协同交联体系来改性胶原纤维,制备了一种具有油水分离性能的胶原纤维基多孔材料。结果表明:该协同交联体系可提高胶原纤维多孔材料的自支撑性、成型性和多孔性,同时未改变胶原的三股螺旋构象并可起到稳定胶原微结构的作用;协同交联改性后胶原纤维多孔材料的收缩温度提升至90℃以上,接触角提升至98°以上,油水分离效率提升至68%以上,表明其热稳定性、疏水性以及油水分离性能均得以改善。 展开更多
关键词 生物质 胶原纤维 多孔材料 协同交联 油水分离 纳米材料
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Macromolecule’s Orientation in a Nanofiber by Bubble Electrospinning
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作者 Dan Tian Danni Yu Chunhui He 《Fluid Dynamics & Materials Processing》 EI 2021年第4期711-720,共10页
In the search for sustainable alternatives to harmful synthetic fibers,an increasing amount of research focuses on biomimicry and natural fibers.Sea silk is an exceptional textile material.It is a kind of natural silk... In the search for sustainable alternatives to harmful synthetic fibers,an increasing amount of research focuses on biomimicry and natural fibers.Sea silk is an exceptional textile material.It is a kind of natural silk produced using the long silky filaments secreted by a specific bivalve mollusk(Pinna nobilis);now at edge of extinction.This paper suggests a simple but effective way to prepare artificial sea silk from Mytilus edulis.A sea silk solution is prepared using a Mytilus edulis protein,and a polyvinyl alcohol(PVA)solution is mixed with the sea silk solution in order to produce artificial sea silk through a bubble electrospinning technique.The effects of the sea silk concentration on the nanofiber’s morphology and mechanical properties are studied experimentally. 展开更多
关键词 MOLLUSK sea-silk solution collagenous fibers bubble electrospinning
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人毛囊真皮鞘细胞移植治疗兔跟腱炎实验研究
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作者 许青 孙香香 +2 位作者 陈锦阳 庄盼 刘军权 《陕西医学杂志》 CAS 2024年第5期583-588,共6页
目的:探讨人毛囊真皮鞘细胞(hHF-DSCs)移植对兔跟腱炎的治疗效果。方法:采集人后枕部毛囊单位,并进行分离培养获得hHF-DSCs。选取30只雄性大白兔随机分为对照组、模型组和治疗组,每组10只。模型组及治疗组于兔左侧跟腱止点上1 cm处多点... 目的:探讨人毛囊真皮鞘细胞(hHF-DSCs)移植对兔跟腱炎的治疗效果。方法:采集人后枕部毛囊单位,并进行分离培养获得hHF-DSCs。选取30只雄性大白兔随机分为对照组、模型组和治疗组,每组10只。模型组及治疗组于兔左侧跟腱止点上1 cm处多点注射Ⅰ型胶原酶建立跟腱炎兔模型。建立跟腱炎模型成功后于第0、3、6、13天,治疗组在兔跟腱患侧注射0.5 ml的hHF-DSCs,模型组与对照组在兔跟腱相同部位注射相同体积0.9%氯化钠溶液。观察各组兔跟腱外观以及组织病理学染色结果。采用ELISA法检测跟腱组织羟脯氨酸(HYP)含量以及肌腱蛋白C(TNC)、基质金属蛋白酶-9(MMP-9)、白细胞介素(IL)-1β、IL-6、IL-10和肿瘤坏死因子-α(TNF-α)表达水平。采用免疫组化法检测跟腱组织Ⅰ、Ⅲ型胶原(collagenⅠ、Ⅲ)表达水平。采用牵拉法检测跟腱组织生物力学。结果:分离培养后的hHF-DSCs表面CD14、CD34、CD45和人类白细胞抗原-DR(HLA-DR)几乎不表达,CD49d、CD90、CD73和CD105表达率接近100%。与对照组比较,模型组步态改变明显,跟腱红肿、无弹性、无光泽。HE染色结果显示,模型组胶原纤维排列疏松,原核细胞较少,分布紊乱不均匀,表明跟腱炎兔模型构建成功。治疗组跟腱组织弹性和光泽度好于模型组,且治疗组HE染色显示胶原纤维排列整齐,无明显纤维坏死,炎症细胞较少,胶原纤维原核细胞数量正常。模型组HYP含量低于对照组和治疗组,对照组高于治疗组(均P<0.05)。模型组MMP-9水平高于对照组和治疗组,治疗组高于对照组(均P<0.05)。模型组TNC水平低于对照组和治疗组,治疗组低于对照组(均P<0.05)。与对照组比较,治疗组和模型组兔跟腱组织IL-1β、IL-6、IL-10及TNF-α表达水平升高(均P<0.05)。与模型组比较,治疗组IL-1β、IL-6、TNF-α表达水平降低(均P<0.05)。与模型组比较,治疗组collagenⅠ、Ⅲ升高(均P<0.05)。与对照组比较,模型组collagenⅠ、Ⅲ降低(均P<0.05)。模型组跟腱最大负荷低于对照组,治疗组高于模型组(均P<0.05)。结论:hHF-DSCs移植治疗兔跟腱炎,能够促进受损跟腱组织修复及胶原合成,降低胶原降解,减轻炎症反应。 展开更多
关键词 跟腱炎 人毛囊真皮鞘细胞 移植 胶原纤维 炎症反应
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多尺度皮革胶原纤维/丁苯橡胶复合材料的制备与性能研究
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作者 姚乃群 卢鑫 +6 位作者 王瀚彬 廖毅彬 刘显奎 王育玲 庞晓燕 丁思博 丁志文 《中国皮革》 CAS 2024年第3期27-32,共6页
利用巯丙基三乙氧基硅烷改性皮革胶原纤维(MCF),并将其作为丁苯橡胶(SBR)的补强剂,以提高丁苯橡胶复合材料的拉伸强度和断裂伸长率。结果表明,基于MCF多尺度结构在增韧中的作用及其与橡胶的界面相互作用,提高了MCF在橡胶中的分散,促进... 利用巯丙基三乙氧基硅烷改性皮革胶原纤维(MCF),并将其作为丁苯橡胶(SBR)的补强剂,以提高丁苯橡胶复合材料的拉伸强度和断裂伸长率。结果表明,基于MCF多尺度结构在增韧中的作用及其与橡胶的界面相互作用,提高了MCF在橡胶中的分散,促进了界面应力传递,从而增强了复合材料的机械性能。与纯SBR相比,填充3%(质量分数)MCF的弹性体复合材料拉伸强度从1.6MPa提高到2.1MPa,断裂伸长率从184%提高到247%。 展开更多
关键词 皮革胶原纤维 硅烷偶联剂 丁苯橡胶 复合材料 机械性能
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年龄对眼周NAD(P)H水平影响及一款眼霜抗皱功效评价
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作者 张小铿 施红 +3 位作者 刘玉 顾晶 许陶敏 范莹 《广东化工》 CAS 2024年第18期183-186,共4页
通过招募一批不同年龄段受试者,使用皮肤无创仪器检测方法分析眼周NAD(P)H水平与年龄、肤色和弹性参数R2之间的相关性。另招募一批受试者,使用一款眼霜评估眼部抗皱效果。研究结果表明:随着年龄增长,眼周颗粒层细胞NAD(P)H水平降低、鱼... 通过招募一批不同年龄段受试者,使用皮肤无创仪器检测方法分析眼周NAD(P)H水平与年龄、肤色和弹性参数R2之间的相关性。另招募一批受试者,使用一款眼霜评估眼部抗皱效果。研究结果表明:随着年龄增长,眼周颗粒层细胞NAD(P)H水平降低、鱼尾纹等级增大,皮肤弹性R2值减小,肤色加深,皮肤ITA°值减小。年龄、肤色、弹性与NAD(P)H水平之间存在相关性。15名受试者使用眼霜28天后,与使用前相比眼周NAD(P)H水平增加,皮肤弹性R2值增加,鱼尾纹评级下降,胶原纤维谐波强度增加。该产品具有一定的抗皱效果。 展开更多
关键词 衰老 皮肤 双光子显微镜 NAD(P)H 胶原纤维
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Remodeling of skin nerve fibers during burn wound healing 被引量:4
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作者 Yongqiang Feng Xia Li +3 位作者 Rui Zhang Yu Liu Tingting Leng Yibing Wang 《Neural Regeneration Research》 SCIE CAS CSCD 2010年第19期1515-1520,共6页
Burn wound healing involves a complex sequence of processes. Recent studies have revealed that skin reinnervation may have an impact on physiological wound repair. Few studies have addressed the process of reinnervati... Burn wound healing involves a complex sequence of processes. Recent studies have revealed that skin reinnervation may have an impact on physiological wound repair. Few studies have addressed the process of reinnervation and morphological changes in regenerated nerve fibers. The regeneration of neurites during full-thickness burn wound healing was determined by immunofluorescent staining using an anti-neurofilament protein monoclonal antibody, and three-dimensional morphology was observed under a laser scanning confocal microscope. Morphology and the volume fraction of collagen and nerve fibers were measured. Skin reinnervation increased during wound healing, peaked during the proliferative scar stage, and then decreased to lower levels during the maturation period. The results from the skin nerve fibers correlated with those from collagen using semi-quantitative analysis. Disintegration and fragmentation were observed frequently in samples from the proliferative stage, and seldom occurred during the maturation stage. There was a remodeling process of regenerated nerve fibers during wound healing, which comprised changed innervation density and topical morphology. The mechanism of remodeling for nerve fibers requires further investigation. 展开更多
关键词 BURNS SCAR wound healing COLLAGEN nerve fibers REINNERVATION tissue remodeling neural regeneration
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