Objective To produce the antiserum of chicken brain derived neuron growth inhibitor collapsin 1. To detect its ability to neutralize the collapsing activity of collapsin 1. Methods The c myc epitope tagged ent...Objective To produce the antiserum of chicken brain derived neuron growth inhibitor collapsin 1. To detect its ability to neutralize the collapsing activity of collapsin 1. Methods The c myc epitope tagged entire sequence of collapsin 1 was amplified by PCR and expression cloned. Rabbits were immunized with the c myc tagged collapsin 1 to produce antiserum of collapsin 1. Its ability to neutralize the collapsing activity of collapsin 1 was observed in dorsal root ganglia (DRG) growth cone. Results Collapsin 1 could induce the collapse of DRG growth cone. The collapsing activity of collapsin 1 could be neutralized by the antiserum of collapsin 1. Conclusion We produced the antibody of a neuron growth inhibitor collapsin 1 that could block its inhibiting function.展开更多
基金Thisprojectissupportedbythenaturalsciencefundofthenation (No 39840 0 11)andthatofJiangsuprovince (BK970 5 7)
文摘Objective To produce the antiserum of chicken brain derived neuron growth inhibitor collapsin 1. To detect its ability to neutralize the collapsing activity of collapsin 1. Methods The c myc epitope tagged entire sequence of collapsin 1 was amplified by PCR and expression cloned. Rabbits were immunized with the c myc tagged collapsin 1 to produce antiserum of collapsin 1. Its ability to neutralize the collapsing activity of collapsin 1 was observed in dorsal root ganglia (DRG) growth cone. Results Collapsin 1 could induce the collapse of DRG growth cone. The collapsing activity of collapsin 1 could be neutralized by the antiserum of collapsin 1. Conclusion We produced the antibody of a neuron growth inhibitor collapsin 1 that could block its inhibiting function.
