Effects of ginsenoside of stem and leaf combined with choline on learning and memory ability of rat models with Alzheimer diseases

BACKGROUND： Central adrenergic nerve and 5-serotonergic nerve can influence central cholinergic nerve on learning and memory and make easy for study; however, ginsenoside of stem and leaf （GSL） can improve functions of central adrenergic nerve; moreover, 5-serotonergic nerve and the combination with choline can produce synergistic effect and enhance learning and memory ability so as to improve learning and memory disorder of patients with Alzheimer disease （AD）. OBJECTIVE ： To observe the effects of GSL combining with choline on learning and memory of AD model rats DESIGN ： Randomized grouping design and controlled animal study SETIING ： Department of Pharmacology, Taishan Medical College MATERIALS ： The experiment was carried out in the Pharmacological Department of Medical College of Jilin University from October 1996 to January 1997. Forty healthy male Wistar rats of clean grade were randomly divided into 5 groups, including sham-injury group, model group, GSL group, choline group and combination group, with 8 rats in each group. Main medications： GSL with the volume more than 92.8% was provided by Department of Chemistry, Norman Bethune Medical College of Jilin University. Panaxatriol, the main component, was detected with thin layer scanning technique and regarded as the index of GSL quality [（55±1）%, CV= 2%, n = 5]. Choline was provided by the Third Shanghai Laboratory Factory. METHODS ： 150 nmol quinolinic acid was used to damage bilateral Meynert basal nuclei of adult rats so as to establish AD models. Rats in GSL, choline and combination groups were intragastric administrated with 400 mg/kg GSL, 200 mg/kg choline （20 mL/kg）, and both respectively last for 17 days starting from two days before operation. Rats in sham-injury group and model group were perfused with the same volume of distilled water once in each morning for the same days. （1） Passive avoidance step-down test： Five minutes later, rats jumped up safe platform when they were shocked with 36 V alternating current. If rats jumped down from the platform and the feet touched railings, the response was wrong. Numbers of wrong response were recorded within 3 minutes, and then the test was redone after 24 hours. （2） Morris water-maze spatial localization task： Swimming from jumping-off to platform directly was regarded as right response. Additionally, 4 successively right responses were regarded as the standard. Each rat was trained 10 times a day with 120 s per time for 3 successive days. The interval was 30 s. Three days later, numbers of right response were recorded. The training times were increased to 30 for unlearned rats. （3） Measurement of activity of choline acetylase in cerebral cortex： Rats were sacrificed at 17 days after operation to obtain cerebral cortex to measure activity of choline acetylase with radiochemistry technique. （4） Synergistic effect： It was expressed as Q value： Q value = factual incorporative effect/anticipant incorporative effect; Q ≥ 1 was regarded as synergistic effect. Anticipant incorporative effect = （EA＋EB-EA·EB）, EA and EB were single timing effect, respectively in GSL group and choline group. E（step-down test and Morris water maze test） = （x in model group - factual value in medicine groups）/x in model group; E （activity of choline acetylase） = （factual value in medicine groups -xin model group）/xin model group. MAIN OUTCOME MEASURES ： （1） Passive avoidance step-down test and Morris water-maze spatial localization task in the study of learning and memory; （2） activity of choline acetylase. RESULTS ： All 40 rats were involved in the final analysis. （1） Passive avoidance response： At learning phase on first day and retesting phase on the next day, numbers of wrong responses within 3 minutes were more in model group than sham operation group, and there was significant difference [（5.88±1.46）, （2.25±0.87） times; （2.63±1.06）, （0.50±0.53） times; P 〈 0.01]; numbers of wrong responses within 3 minutes were less in combination group than model group, and there was significant difference [learning phase： （1.12±0.83）, （5.88±1.46） times; retesting phase： （0.38±0.74）, （2.63±1.06）times, P 〈 0.01]; moreover, effect was stronger than that in GSL group and choline group. The Q value was 1.07 and 1.59, respectively and it showed synergistic effect. Spatial localization task： Training times were more in model group than sham operation group, and there was significant difference [（2.9±2.5）, （12.6±3.5） times; P 〈 0.01]. Training times were less in combination group than model group, and there was significant difference [（11.8±2.4）, （27.9±2.5） times, P 〈 0.01]; moreover, effect was stronger than that in GSL group and choline group. The Q value was 1.07 and it showed synergistic effect. （3） Activity of choline acetylase： Activity was lower in model group than sham operation group, and there was significant difference [（30.56±8.33）, （61.11 ±8.33） nkat/g; P 〈 0.01]. Activity was higher in combination group than model group and there was significant difference [（50.00±8.33）, （30.56±8.33） nkat/g, P 〈 0.01];moreover, effect was stronger than that in GSL group and choline group. The Q value was 1.5 and it showed synergistic effect. CONCLUSZON： GSL in combination with choline can synergically improve the disorder of learning and memory of AD model rats. Its mechanism may be involved in enhancing the function of central cholinergic system.

Combined refraction and diffraction models for sea waves over complicated topography and with currents in shallow water

-Combined refraction and diffraction models in the form of linear parabolic approximation are derived through smallparameter method. More strictly theoretical basis and more accuracy in the models than Lozano's (1980) are obtained. Some theoretical defects in Liu's model (1985) with consideration of current are not only found but also eliminated. More strict and accurate models are, therefore, presented in this paper.The calculation results and analysis in applying the models to actual wave field with consideration of bottom friction will be given in the following paper.

Combined model of trip mode and destination

This paper analyzes the characteristics of the destination distribution of trips and proposes a stratified sampling strategy for travel mode choice.The stratified sampling strategy can reduce the size of the alternative set;thus,the computation burden of simulation is decreased.Using the stratified sampling strategy,a combined choice model of the trip mode and destination is developed based on the Bayesian theory.Simulations are carried out to verify the proposed model.The results show that the combined choice model of the trip mode and destination can efficiently simulate travelers＇ choice behaviors.Furthermore,the forecasting accuracy of the combined choice model is higher than the one of the gravity model.Therefore,the proposed model is a powerful tool with which to analyze travelers＇ behaviors in selecting the trip mode.

Combined model based on optimized multi-variable grey model and multiple linear regression

The construction method of background value is improved in the original multi-variable grey model （MGM（1,m）） from its source of construction errors. The MGM（1,m） with optimized background value is used to eliminate the random fluctuations or errors of the observational data of all variables, and the combined prediction model together with the multiple linear regression is established in order to improve the simulation and prediction accuracy of the combined model. Finally, a combined model of the MGM（1,2） with optimized background value and the binary linear regression is constructed by an example. The results show that the model has good effects for simulation and prediction.

Combined Prediction for Vehicle Speed with Fixed Route

Achieving accurate speed prediction provides the most critical support parameter for high-level energy management of plug-in hybrid electric vehicles.Nowadays,people often drive a vehicle on fixed routes in their daily travels and accurate speed predictions of these routes are possible with random prediction and machine learning,but the prediction accuracy still needs to be improved.The prediction accuracy of traditional prediction algorithms is difficult to further improve after reaching a certain accuracy;problems,such as over fitting,occur in the process of improving prediction accuracy.The combined prediction model proposed in this paper can abandon the transitional dependence on a single prediction.By combining the two prediction algorithms,the fusion of prediction performance is achieved,the limit of the single prediction performance is crossed,and the goal of improving vehicle speed prediction performance is achieved.In this paper,an extraction method suitable for fixed route vehicle speed is designed.The application of Markov and back propagation(BP)neural network in predictions is introduced.Three new combined prediction methods,all named Markov and BP Neural Network(MBNN)combined prediction algorithm,are proposed,which make full use of the advantages of Markov and BP neural network algorithms.Finally,the comparison among the prediction methods has been carried out.The results show that the three MBNN models have improved by about 19%,28%,and 29%compared with the Markov prediction model,which has better performance in the single prediction models.Overall,the MBNN combined prediction models can improve the prediction accuracy by 25.3%on average,which provides important support for the possible optimization of plug-in hybrid electric vehicle energy consumption.

A combined numerical tidal model for the Hangzhou Bay and Qiantang River

-In order to avoid prescribing open boundary condition on the upstream side of the Hangzhou Bay, in numerical simulation of the tides and residual currents of the Bay, a 1-D model for the Qiantang River is connected to the 2-D model for the Hangzhou Bay. The harmonic constants of diurnal constituent [ (K1+O1)/2],semidiurnal constituent (M2) and shallow water constituent (M4) are obtained. The results produced by the combined model are in better agreement with the observed ones than those produced solely by the original 2-D model. The combined model gives much more reliable results for tide-induced residual water level and current.

Study of establishing disease-syndrome combined with animal model for immune thrombocytopenic purpura without additional conditions

Objective:To explore the feasibility of establishing the disease-syndrome combined animal model for immune thrombocytopenic purpura(ITP)without additional conditions.Methods:Three batches of data related to the ITP model mice obtained by replication at different time were analyzed,and whether the APS-injected model mice replicated through the passive immune modeling method could simulate the pathogenesis and clinical characteristics of human ITP was evaluated according to the differentiation criteria for diseasesyndrome combined model.Results:The APS-injected replicated ITP model mice possessed the following traits:(1)Compared with the normal group,the platelet count was significantly decreased,and coagulation time was significantly increased in the model group(P<.01).(2)Compared with the normal group,the medullary thrombocytogenous megakaryocytes were significantly decreased(P<.05,.01,.001).(3)The APS-injected sites and other parts of the model mice had spontaneous hemorrhage.(4)Behavioral changing signs were observed 1 week after the modeling(i.e.low activity,delayed activity,poor appetite,skin petechia/hemorrhage and spontaneous hemorrhage at the injected sites or other parts),and were getting more and more severe.Conclusion:According to the syndrome differentiation criteria for disease-syndrome combined model of ITP,the APS-injected animal model of ITP replicated through the passive immune modeling method without additional conditions possesses the characteristics of disease-syndrome combined model.It provides an ideal tool for the development of traditional Chinese medicine pharmacology experiment.

Combined hybrid energy storage system and transmission grid model for peak shaving based on time series operation simulation

This study proposes a combined hybrid energy storage system(HESS) and transmission grid(TG) model, and a corresponding time series operation simulation(TSOS) model is established to relieve the peak-shaving pressure of power systems under the integration of renewable energy. First, a linear model for the optimal operation of the HESS is established, which considers the different power-efficiency characteristics of the pumped storage system, electrochemical storage system, and a new type of liquid compressed air energy storage. Second, a TSOS simulation model for peak shaving is built to maximize the power entering the grid from the wind farms and HESS. Based on the proposed model, this study considers the transmission capacity of a TG. By adding the power-flow constraints of the TG, a TSOS-based HESS and TG combination model for peak shaving is established. Finally, the improved IEEE-39 and IEEE-118 bus systems were considered as examples to verify the effectiveness and feasibility of the proposed model.

A COMBINED MODEL OF WIND, WAVE, TIDE AND STORM SURGES

A combined numerical model of wind, wave, tide, and storm surges was built on the basis of the “wind field model in limited sea surface areas”. When used to forecast the sea surface wind, wave height and water level, it can describe them very well.

ARMA-GM combined forewarning model for the quality control

Three forecasting models are set up: the auto\|regressive moving average model, the grey forecasting model for the rate of qualified products P t, and the grey forecasting model for time intervals of the quality catastrophes. Then a combined forewarning system for the quality of products is established, which contains three models, judgment rules and forewarning state illustration. Finally with an example of the practical production, this modeling system is proved fairly effective.

Evaluation of the Application Effect of Short Video Combined with BOPPPS Teaching Mode in Clinical Anesthesia Teaching

Objective:To study the application effect of short video combined with BOPPPS teaching mode in clinical anesthesia practice.Method:48 students assigned to clinical anesthesia in digestive endoscopy of Shanxi Bethune Hospital from July 1,2022 to April 1,2023 were selected as research objects.They were randomly divided into the control group(PowerPoint presentation teaching group)and the observation group(short video combined with BOPPPS teaching group),with 24 students in each group.After the internship,the students’theoretical and technical scores were tested,the effects of the two teaching modes were compared,and the students’satisfaction was investigated.Results:The test scores of students in the observation group were significantly better than those in the control group(P<0.05).The short video combined with BOPPPS teaching mode can significantly improve students’learning interest,operation skills,and memory(P<0.05).The students’satisfaction in the observation group was higher than that in the control group(P<0.05).Conclusion:In clinical practice,the application of short video combined with BOPPPS teaching mode has achieved great effect,which is worth further promotion and research.

Combined bottleneck effect of on-ramp and bus stop in a cellular automaton model

The combined bottleneck effect is investigated by modeling traffic systems with an on-ramp and a nearby bus stop in a two-lane cellular automaton model. Two cases, i.e. the bus stop locates in the downstream section of the on-ramp and the bus stop locates in the upstream section of the on-ramp, are considered separately. The upstream flux and downstream flux of the main road, as well as the on-ramp flux are analysed in detail, with respect to the entering probabilities and the distance between the on-ramp and the bus stop. It is found that the combination of the two bottlenecks causes the capacity to drop off, because the vehicles entering the main road from the on-ramp would interweave with the stopping （pulling-out） buses in the downstream （upstream） case. The traffic conflict in the former case is much heavier than that in the latter, causing the downstream main road to be utilized inefficiently. This suggests that the bus stop should be set in the upstream section of the on-ramp to enhance the capacity. The fluxes both on the main road and on the on-ramp vary with the distance between the two bottlenecks in both cases. However, the effects of distance disappear gradually at large distances. These findings might give some guidance to traffic optimization and management.

医院季度收入预测的比较研究

目的 探讨具有季度波动和长期趋势特征事物预测问题的方法论,为卫生领域同类预测问题提供定量决策方法论依据。方法 以某所医院季度收入预测作为实证算例载体,分别用乘积季节SARIMA法、X11-ARIMA法、X11-曲线函数外推法建立简单组合模型以及二次加权组合模型。结果 乘积季节SARIMA模型、X11-ARIMA模型、X11-二次多项式的平均相对误差分别为1.28%、0.96%、1.11%。对以上简单组合模型继续进行深度加权组合,预测误差为0.70%,模型预测性能更优。结论 SARIMA模型、X11-ARIMA模型、X11-二次多项式以及二次组合模型对于局部周期性波动、全局稳定性趋势的时序数据资料拟合效果不错;对于卫生管理领域预测问题有适用性,鉴于多种匹配方法原理不同、信息利用差异大,基于多种方法的二次组合模型取得更优性能。

山东省中医类医院卫生人力资源需求预测

目的了解山东省中医类医院卫生人力资源的现状,预测卫生人力资源未来的需求量并提出合理建议,以期为相关部门制定中医药人力资源规划提供依据和数据支持。方法运用差分自回归移动平均(auto-regressive moving average,ARIMA)模型、灰色系统预测模型(grey system forecasting model,GM)中的GM(1,1)模型以及两者的线性组合模型预测2021—2025年山东省中医类医院卫生人力资源需求量,比较不同模型预测的精准度。结果组合模型的系统误差小,预测效果最好;卫生技术人员、执业(助理)医师、中医类别执业(助理)医师、注册护士、药师(士)及中药师(士)2025年对应的人力资源预测值分别是107457人、43304人、22807人、51372人、5718人、3242人。结论山东省中医类别执业(助理)医师数量储备充足,但中药师(士)相对短缺,人才结构不合理,医护比有待优化。建议政府适当地增加中药师(士)的编制,促进执业(助理)医师与中药师(士)平衡发展;增加对中医类医院的财政拨款,加强人才引进力度,创新人才培养机制,优化山东省中医药人才结构;制定科学合理的排班制度,提高护士的社会地位,进一步优化医护比。

Accurate and rapid error estimation on global gravitational field from current GRACE and future GRACE Follow-On missions

Firstly, the new combined error model of cumulative geoid height influenced by four error sources, including the inter-satellite range-rate of an interferometric laser （K-band） ranging system, the orbital position and velocity of a global positioning system （GPS） receiver and non-conservative force of an accelerometer, is established from the perspectives of the power spectrum principle in physics using the semi-analytical approach. Secondly, the accuracy of the global gravitational field is accurately and rapidly estimated based on the combined error model; the cumulative geoid height error is 1.985× 10^-1 m at degree 120 based on GRACE Level 1B measured observation errors of the year 2007 published by the US Jet Propulsion Laboratory （JPL）, and the cumulative geoid height error is 5.825 × 10^-2 m at degree 360 using GRACE Follow-On orbital altitude 250 km and inter-satellite range 50 km. The matching relationship of accuracy indexes from GRACE Follow-On key payloads is brought forward, and the dependability of the combined error model is validated. Finally, the feasibility of high-accuracy and high-resolution global gravitational field estimation from GRACE Follow-On is demonstrated based on different satellite orbital altitudes.

Forecasting China’s natural gas consumption based on a combination model

Ensuring a sufficient energy supply is essential to a country. Natural gas constitutes a vital part in energy supply and therefore forecasting natural gas consumption reliably and accurately is an essential part of a country＇s energy policy. Over the years, studies have shown that a combinative model gives better projected results compared to a single model. In this study, we used Polynomial Curve and Moving Average Combination Projection （PCMACP） model to estimate the future natural gas consumption in China from 2009 to 2015. The new proposed PCMACP model shows more reliable and accurate results： its Mean Absolute Percentage Error （MAPE） is less than those of any previous models within the investigated range. According to the PCMACP model, the average annual growth rate will increase for the next 7 years and the amount of natural gas consumption will reach 171600 million cubic meters in 2015 in China.

Micro Mechanical Model of 3D Woven Composites

A combined beam model representing the periodicity of the microstructure and micro deformation of 3D woven composites is developed for predicting mechanical properties. The model considers the effects of off axial tension/compression and bending/shearing couplings as well as the mutual reactions of fiber yarns. The method determining microstructure by using woven parameters is described for a typical 3D woven composite material. An analytical cell, constructed by a minimum periodic section of yarn and interlayer matrix, is adopted. Micro stresses in the cell under in-plane tensile loading are obtained by using the proposed beam model and macro modulus is then obtained by the averaging method. Material tests and a 2D micro FEM analysis are made to evaluate this model. Analyses reveal that micro stress caused by tensile/bending coupling effect is not negligible in the stress analysis.

A Novel Approach for QoS Prediction Based on Bayesian Combinational Model

As an important factor in evaluating service,QoS(Quality of Service) has drawn more and more concerns with the rapid increasing of Web services. However,due to the great volatility of services in Mobile Internet environments,such as internet of vehicles,Web services often do not work as announced and thus cause unacceptable problems. QoS prediction can avoid failure before it takes place,which is considered a more effective way to assure quality. However,Current QoS prediction approaches neither consider the highly dynamic of Web services,nor maintain good prediction performance all the time. Consequently we propose a novel Bayesian combinational model to predict QoS by continuously adjusting credit values of the basic models so as to keep good prediction accuracy. QoS attributes such as response time,throughput and reliability are used to validate the proposed model. Experimental results show that the model can provide stable prediction results in Mobile Internet environments.

Predicting Surface Urban Heat Island in Meihekou City, China: A Combination Method of Monte Carlo and Random Forest

Given the rapid urbanization worldwide, Urban Heat Island(UHI) effect has been a severe issue limiting urban sustainability in both large and small cities. In order to study the spatial pattern of Surface urban heat island(SUHI) in China’s Meihekou City, a combination method of Monte Carlo and Random Forest Regression(MC-RFR) is developed to construct the relationship between landscape pattern indices and Land Surface Temperature(LST). In this method, Monte Carlo acceptance-rejection sampling was added to the bootstrap layer of RFR to ensure the sensitivity of RFR to outliners of SUHI effect. The SHUI in 2030 was predicted by using this MC-RFR and the modeled future landscape pattern by Cellular Automata and Markov combination model(CA-Markov). Results reveal that forestland can greatly alleviate the impact of SUHI effect, while reasonable construction of urban land can also slow down the rising trend of SUHI. MC-RFR performs better for characterizing the relationship between landscape pattern and LST than single RFR or Linear Regression model. By 2030, the overall SUHI effect of Meihekou will be greatly enhanced, and the center of urban development will gradually shift to the central and western regions of the city. We suggest that urban designer and managers should concentrate vegetation and disperse built-up land to weaken the SUHI in the construction of new urban areas for its sustainability.

Traffic flow prediction of urban road network based on LSTM-RF model

Traffic flow prediction,as the basis of signal coordination and travel time prediction,has become a research point in the field of transportation.For traffic flow prediction,researchers have proposed a variety of methods,but most of these methods only use the time domain information of traffic flow data to predict the traffic flow,ignoring the impact of spatial correlation on the prediction of target road segment flow,which leads to poor prediction accuracy.In this paper,a traffic flow prediction model called as long short time memory and random forest(LSTM-RF)was proposed based on the combination model.In the process of traffic flow prediction,the long short time memory(LSTM)model was used to extract the time sequence features of the predicted target road segment.Then,the predicted value of LSTM and the collected information of adjacent upstream and downstream sections were simultaneously used as the input features of the random forest model to analyze the spatial-temporal correlation of traffic flow,so as to obtain the final prediction results.The traffic flow data of 132 urban road sections collected by the license plate recognition system in Guiyang City were tested and verified.The results show that the method is better than the single model in prediction accuracy,and the prediction error is obviously reduced compared with the single model.