Taxonomic Status of Daduhe Loquat （Eriobotrya prinoides var. dadunensis）

The taxonomic status of Daduhe loquat （E. prinoides var. dadunensis） was studied through analyzing genetic relationships among Oakleaf loquat （E. prinoides）, Daduhe loquat and Common loquat （E. japonica） using inter simple sequence repeats （ISSR） molecular marker and morphologic marker in this paper. Based on ISSR marker research, the similarity coefficient between Oakleaf loquat and Common loquat was lower than the similarity coefficient between Oakleaf loquat and Daduhe loquat while the similarity coefficient between Daduhe loquat and Common loquat was intermediate. The highest additivity was obtained when Daduhe loquat was regarded as the undetermined hybrid （45.8%）. The specific bands of Oakleaf loquat and Common loquat were present in Daduhe loquat. Based on morphologic traits research, Daduhe loquat was also between Oakleaf loquat and Common loquat but a little leaning to Oakleaf loquat. All the results support that Daduhe loquat was hybrid of Oakleaf loquat and Common loquat.

两种枇杷叶片皂苷含量测定和比较研究

采用香草醛-高氯酸法直接测定比较香花枇杷和普通枇杷两种样品中皂苷含量的差异。结果表明:两种叶片不同萃取部的皂苷含量差异较大,在极性较大的浓缩液浸膏、正丁醇部及剩余水相部等萃取部,香花枇杷的皂苷含量高于普通枇杷,可能存在潜在药用价值。

普通枇杷、大渡河枇杷和栎叶枇杷遗传关系研究——基于RAPD和AFLP分析

利用筛选得到的24个随机引物和5对AFLP引物组合对普通枇杷、大渡河枇杷及栎叶枇杷的遗传关系进行RAPD及AFLP分析,结果表明,普通枇杷与栎叶枇杷的相似性系数最小(0.3864和0.5364),而大渡河枇杷介于普通枇杷和栎叶枇杷之间,偏向于栎叶枇杷。分别以普通枇杷、大渡河枇杷和栎叶枇杷作为待定杂种,计算了三者谱带的叠加性,结果表明,无论是RAPD分子标记,还是AFLP分子标记,以大渡河枇杷作待定杂种获得最高叠加性,支持大渡河枇杷可能是普通枇杷与栎叶枇杷的杂交种的结论。

香花枇杷与普通枇杷叶片提取物抑菌效果和抗氧化活性的比较研究

以香花枇杷和普通枇杷叶片提取物为实材,采用牛津杯抑菌实验、最小抑菌浓度(MIC)的测定、DPPH自由基清除率的测定对其不同萃取部分进行了抑菌效果和抗氧化活性的研究,以期比较这两个种类的抑菌和抗氧化活性。结果表明:对于供实菌,7个样品中抑菌效果最好的溶剂萃取部分是7#(上清液正丁醇部)、最差的是3#(沉淀乙酸乙酯部);与阳性对照山梨酸钾相比,香花枇杷和普通枇杷的不同萃取部位的最小抑菌浓度(MIC)更小,抑菌效果更好;极性较强的5#(沉淀萃取剩余部)、6#(上清液乙酸乙酯部)、7#(上清液正丁醇部萃取部)是抗氧化活性较强的部位。总体上,香花枇杷和普通枇杷相比,活性成分有较大的相似性,但具有更好的抑菌效果与抗氧化活性,可能存在潜在药用价值,有着较为广阔的应用前景。