Microbial community structure and functional metabolic diversity are associated with organic carbon availability in an agricultural soil

Exploration of soil environmental characteristics governing soil microbial community structure and activity may improve our understanding of biogeochemical processes and soil quality. The impact of soil environmental characteristics especially organic carbon availability after 15-yr different organic and inorganic fertilizer inputs on soil bacterial community structure and functional metabolic diversity of soil microbial communities were evaluated in a 15-yr fertilizer experiment in Changping County, Beijing, China. The experiment was a wheat-maize rotation system which was established in 1991 including four different fertilizer treatments. These treatments included： a non-amended control（CK）, a commonly used application rate of inorganic fertilizer treatment（NPK）; a commonly used application rate of inorganic fertilizer with swine manure incorporated treatment（NPKM）, and a commonly used application rate of inorganic fertilizer with maize straw incorporated treatment（NPKS）. Denaturing gradient gel electrophoresis（DGGE） of the 16 S r RNA gene was used to determine the bacterial community structure and single carbon source utilization profiles were determined to characterize the microbial community functional metabolic diversity of different fertilizer treatments using Biolog Eco plates. The results indicated that long-term fertilized treatments significantly increased soil bacterial community structure compared to CK. The use of inorganic fertilizer with organic amendments incorporated for long term（NPKM, NPKS） significantly promoted soil bacterial structure than the application of inorganic fertilizer only（NPK）, and NPKM treatment was the most important driver for increases in the soil microbial community richness（S） and structural diversity（H）. Overall utilization of carbon sources by soil microbial communities（average well color development, AWCD） and microbial substrate utilization diversity and evenness indices（H＇ and E） indicated that long-term inorganic fertilizer with organic amendments incorporated（NPKM, NPKS） could significantly stimulate soil microbial metabolic activity and functional diversity relative to CK, while no differences of them were found between NPKS and NPK treatments. Principal component analysis（PCA） based on carbon source utilization profiles also showed significant separation of soil microbial community under long-term fertilization regimes and NPKM treatment was significantly separated from the other three treatments primarily according to the higher microbial utilization of carbohydrates, carboxylic acids, polymers, phenolic compounds, and amino acid, while higher utilization of amines/amides differed soil microbial community in NPKS treatment from those in the other three treatments. Redundancy analysis（RDA） indicated that soil organic carbon（SOC） availability, especially soil microbial biomass carbon（Cmic） and Cmic/SOC ratio are the key factors of soil environmental characteristics contributing to the increase of both soil microbial community structure and functional metabolic diversity in the long-term fertilization trial. Our results showed that long-term inorganic fertilizer and swine manure application could significantly improve soil bacterial community structure and soil microbial metabolic activity through the increases in SOC availability, which could provide insights into the sustainable management of China＇s soil resource.

＂Beyond the Dreams of Loveliness＂ --Film, Unionism, and Working Class Community Formation in Detroit, 1933-1939

Ever since Lawrence Goodwyn published The Populist Moment, social historians have been keenly aware of the power culture represents when it comes to resistance. Historically, ＂movement culture＂ has been used to create a collective climate and offered political activists a springboard to discuss with the masses the benefits of joining a social movement. ＂Beyond the Dreams of Loveliness＂ uses the cultural outlet of film and the labor movement in the city of Detroit as analytical tools to examine working class community resistance throughout the 1930s. In the midst of the ＂golden age of film＂ community activists and union organizers--some of whom were well-known radicals and some of whom were mainstream unionists--used the institution of cinema to instill a level of class consciousness in the masses and mobilize the working class community against exploitation of employers, reactionary politicians, and white supremacist organizations. Movie-going had been a very popular form of recreation in Detroit since the 1920s and downtown theatres increasingly became the destination of an eclectic array of workers. Movie theaters themselves were bastions of community activity around the thirties and many were located in the heart of the city＇s working class district. Yet it was the films themselves that offered community activists and unionists a chance to converse with workers regarding the socio-economic matters of the day. Hollywood released a good number of films that dealt with working class issues and provided activists with ample opportunities to ＂instruct＂ the audience and carry on the discussion into the factory or union halls. ＂Beyond the Dreams of Loveliness＂ relies on a combination of oral histories, movie reviews, film clips, and union and/or working class organizational records to illuminate how activists in Detroit＇s working class community used film as an outlet to inspire working class resistance. In sum, film provided an accessible form of entertainment that proved to be effective on two fronts： It promoted increased group cooperation, friendship, and alliances while it also fostered a shared culture, making it easier for activists to organize workers in the coming struggles for unionism.

Participatory and integrated watershed management and community based organgzanon

In Iran, and in order to encourage local watershed management, community based organizations （CBO） like ＂cooperatives of watershed＂, ＂construction groups＂ and ＂watershed management groups＂ have been established to obtain resident＇s cooperation in activities eormeeted with protection, reclamation and exploitation of natural resources in the basins that lead to sustainability within watershed management. This study was done as a ease study to explore the role of Remeleh Group （RG） as a CBO in the management of the ＂Kharkheh＂ sub-basin. Participatory action and learning method was used. Participatory tools including natural resources mapping, flow diagram, transect walk, semi-structured interview and brainstorming were applied. Making the conclusion, it was found that the CBO of Remeleh Group cause： （1） Enhancement of social capital through encouraging cooperation and participatory practices, creating new communication among local residents and outsiders, reducing conflicts and promoting the solidarity among communities in the sub basin of Karldaeh; （2） Sustain livelihood and economic situation through agricultural intensification and livelihood diversification （gardening and agriculture together and reducing migration）; （3） Conservation of environmental and natural recourses via the implementation of watershed activities like vine, terracing, bunding and destocking, seedling, and check dams; （4） Empower local people through carrying out training on the watershed practices.

Seed Size as Key Factor in Germination and Seedling Development of Copaifera langsdorffii(Fabaceae)

Seed size is a plastic trait of the plants that directly affect seed germination and seedling recruitment. This study aimed to investigate the relationship between seed size, seed germinability and seedling performance of Copaifera langsdorffii by testing four hypotheses: 1) larger seeds have higher germination percentage;2) smaller seeds require less time to germinate and for initial development of the seedlings;3) larger seeds produce more vigorous seedlings and 4) seed size negatively affects seedling root/shoot ratio. In 2011, we selected 30 individuals of C. langsdorffii from which 300 seeds were randomly collected in the plant canopy. All these seeds were weighted and placed in germination tray using vermiculite as substrate. Seed germinability and initial development of seedlings were monitored daily until cotyledons fell. Small seeds have higher germination percentage and germinate faster when compared to large seeds. Nonetheless, seedlings originated from larger seeds have longer development times, resulting in more vigorous seedlings. In addition, seedlings originating from small seeds allocate proportionally greater amount of resources to roots when compared to larger seeds. The fact that small seeds have higher germination percentage and faster germination favors the colonization of transient habitats. However, larger seeds produce more vigorous seedlings, favoring the seedling establishment in more stable habitats. Thus, we argue that high variability in seed size of C. langsdorffii favors its widespread geographic distribution.

Control systems and data management for high-power laser facilities

The next generation of high-power lasers enables repetition of experiments at orders of magnitude higher frequency than what was possible using the prior generation.Facilities requiring human intervention between laser repetitions need to adapt in order to keep pace with the new laser technology.A distributed networked control system can enable laboratory-wide automation and feedback control loops.These higher-repetition-rate experiments will create enormous quantities of data.A consistent approach to managing data can increase data accessibility,reduce repetitive data-software development and mitigate poorly organized metadata.An opportunity arises to share knowledge of improvements to control and data infrastructure currently being undertaken.We compare platforms and approaches to state-of-the-art control systems and data management at high-power laser facilities,and we illustrate these topics with case studies from our community.

Comparison of residents' committees in two Chinese cities:Shanghai and Shenyang

The residents＇ committee as an affiliate of the sub-district office actually plays a role much greater than that of a ＂self-governing mass organization＂ as defined by law. Owing to path dependence arising from their long-time role as agents of the government, residents＇ committee staff are naturally used to regarding daily political administration closely related to community development as part of internal community affairs. Therefore, community building is essentially the construction of a governance structure in grassroots society, which entails state forces vacating some space to allow the self-operation of grass-roots society. To realize this goal, the critical precondition rests in that the management and services of the functional departments of government must hit the mark. Creative institutional reform involves establishing a community governance system consisting of a ＂community-building guidance committee （government agency）, community management committee （social association）, community workers （professional service body）, community residents＇ committee （community self-governing organization）.＂

Volatile organic compound emissions from straw-amended agricultural soils and their relations to bacterial communities:A laboratory study

A laboratory study was conducted to investigate volatile organic compound（VOC） emissions from agricultural soil amended with wheat straw and their associations with bacterial communities for a period of 66 days under non-flooded and flooded conditions. The results indicated that ethene, propene, ethanol, i-propanol, 2-butanol, acetaldehyde, acetone,2-butanone, 2-pentanone and acetophenone were the 10 most abundant VOCs, making up over 90% of the total VOCs released under the two water conditions. The mean emission of total VOCs from the amended soils under the non-flooded condition（5924 ng C/（kg·hr）） was significantly higher than that under the flooded condition（2211 ng C/（kg·hr））. One ＂peak emission window＂ appeared at days 0–44 or 4–44, and over 95% of the VOC emissions occurred during the first month under the two water conditions. Bacterial community analysis using denaturing gradient gel electrophoresis（DGGE） showed that a relative increase of Actinobacteria, Bacteroidetes, Firmicutes and γ-Proteobacteria but a relative decrease of Acidobacteria with time were observed after straw amendments under the two water conditions. Cluster analysis revealed that the soil bacterial communities changed greatly with incubation time, which was in line with the variation of the VOC emissions over the experimental period. Most of the above top 10 VOCs correlated positively with the predominant bacterial species of Bacteroidetes, Firmicutes and Verrucomicrobia but correlated negatively with the dominant bacterial species of Actinobacteria under the two water conditions. These results suggested that bacterial communities might play an important role in VOC emissions from straw-amended agricultural soils.