γ-Secretase,called“the proteasome of the membrane,”is a membrane-embedded protease complex that cleaves 150+peptide substrates with central roles in biology and medicine,including amyloid precursor protein and the ...γ-Secretase,called“the proteasome of the membrane,”is a membrane-embedded protease complex that cleaves 150+peptide substrates with central roles in biology and medicine,including amyloid precursor protein and the Notch family of cell-surface receptors.Mutations inγ-secretase and amyloid precursor protein lead to early-onset familial Alzheimer’s disease.γ-Secretase has thus served as a critical drug target for treating familial Alzheimer’s disease and the more common late-onset Alzheimer’s disease as well.However,critical gaps remain in understanding the mechanisms of processive proteolysis of substrates,the effects of familial Alzheimer’s disease mutations,and allosteric modulation of substrate cleavage byγ-secretase.In this review,we focus on recent studies of structural dynamic mechanisms ofγ-secretase.Different mechanisms,including the“Fit-Stay-Trim,”“Sliding-Unwinding,”and“Tilting-Unwinding,”have been proposed for substrate proteolysis of amyloid precursor protein byγ-secretase based on all-atom molecular dynamics simulations.While an incorrect registry of the Notch1 substrate was identified in the cryo-electron microscopy structure of Notch1-boundγ-secretase,molecular dynamics simulations on a resolved model of Notch1-boundγ-secretase that was reconstructed using the amyloid precursor protein-boundγ-secretase as a template successfully capturedγ-secretase activation for proper cleavages of both wildtype and mutant Notch,being consistent with biochemical experimental findings.The approach could be potentially applied to decipher the processing mechanisms of various substrates byγ-secretase.In addition,controversy over the effects of familial Alzheimer’s disease mutations,particularly the issue of whether they stabilize or destabilizeγ-secretase-substrate complexes,is discussed.Finally,an outlook is provided for future studies ofγ-secretase,including pathways of substrate binding and product release,effects of modulators on familial Alzheimer’s disease mutations of theγ-secretase-substrate complexes.Comprehensive understanding of the functional mechanisms ofγ-secretase will greatly facilitate the rational design of effective drug molecules for treating familial Alzheimer’s disease and perhaps Alzheimer’s disease in general.展开更多
Here,we characterize the temporal and spatial dynamics of forest community structure and species diversity in a subtropical evergreen broad-leaved forest in China.We found that community structure in this forest chang...Here,we characterize the temporal and spatial dynamics of forest community structure and species diversity in a subtropical evergreen broad-leaved forest in China.We found that community structure in this forest changed over a 15-year period.Specifically,renewal and death of common species was large,with the renewal of individuals mainly concentrated within a few populations,especially those of Aidia canthioides and Cryptocarya concinna.The numbers of individual deaths for common species were concentrated in the small and mid-diameter level.The spatial distribution of community species diversity fluctuated in each monitoring period,showing a more dispersed diversity after the 15-year study period,and the coefficient of variation on quadrats increased.In 2010,the death and renewal of the community and the spatial variation of species diversity were different compared to other survey years.Extreme weather may have affected species regeneration and community stability in our subtropical monsoon evergreen broad-leaved forests.Our findings suggest that strengthening the monitoring and management of the forest community will help better understand the long-and short-term causes of dynamic fluctuations of community structure and species diversity,and reveal the factors that drive changes in community structure.展开更多
Aim: This paper aims to evaluate disparities of type 2 diabetes structured health education programmes that is utilised within the communities. Design: systematic review, (a type of secondary research design) aiming t...Aim: This paper aims to evaluate disparities of type 2 diabetes structured health education programmes that is utilised within the communities. Design: systematic review, (a type of secondary research design) aiming to summarize the results of prior primary research studies on available evidence Community type 2 diabetes structured education (CT2DSHE). Methods: Research question: Type 2 diabetic structured health education within a community how effective is it? Qualitative Systematic review, defined as a way to get reliable and objective picture of current available evidence on the specific topic—(CT2DSHE), (Denscombe, 2021) through reflexivity synthesis of available data as an example. This is valuable in time constraints such as project assignments that must be met within specific time and also to bring together available evidence together [1]. Results: This review has shown that CT2DSHE is effective with seven out of the eleven authors supporting, three authors against and one was neutral, further showed that knowledge and skills acquired can last longer with patient activation improved among T2DM patients ideal for sustaining their self-management of T2DM. Conclusion: This research provides suggestive answers to the research question: “Type 2 diabetic structured health education within a community how effective is it?”, This has demonstrated CT2DSHE effectiveness in knowledge acquisition and improving T2DM awareness among T2DM patients, whilst evidencing long effects beyond the study times of 3 - 9 months period in relation to patient activation. Also Identified diabetes education self-management on newly diagnosed (DESMOND) patient as CT2DSHE program for recommendation. Patient or Public Contribution: This work aspires to contribute to CT2DSHE in these areas;Influencing policy decision-making for community diabetes care within the UK and world at large., Contributing to already vast knowledge on diabetes self-management and reasons why?, Influencing educators on how CT2DSHEP are designed, delivered by putting the patient at the Centre and bringing different perspectives on CT2DSHEP in one place that is serving users time of having to consult several resources especially busy clinicians [2] [3].展开更多
Five years' (2000-2004) continuous study has been carried out on small mammals such as rodents in seven different sample plots, at three different altitudes and in six different ecological environment types in the ...Five years' (2000-2004) continuous study has been carried out on small mammals such as rodents in seven different sample plots, at three different altitudes and in six different ecological environment types in the eastern part of the Wuling Mountains, south bank of the Three Gorges of Yangtze River in Hubei. A total of 29 297 rat clamps/times were placed and 2271 small mammals such as rodents were captured, and 26 small mammals were captured by other means. All the small mammals captured belonged to 8 families 19 genera and 24 species, of which rodentia accounted for 70.83% and insectivora 29.17%. Through analysis of the data, the results showed that: 1 ) although the species richness had a trend of increasing along different sample plots as altitude increased from south to north, quite a few species showed a wide habitat range in a vertical distribution ( 15 species were dispersed over three zones and two species over two zones) , indicating a strong adaptability of small mammals such as rOdents at lower altitudes in most areas and comparatively less vertical span of entire mountains; 2) whether in seven different sample plots or six different ecological types, Apodemus agrarius and Rattus norvegicus were dominant species below 1200m, and Anourosorex squamipes, Niviventer confucianus and Apodemus draco were dominant above altitudes of 1300m, however, in quantity they were short of identical regularity, meaning they did not increase as the altitude did, or decrease as the ecological areas changed; 3)the density in winter was obviously greater than that in spring, and the distribution showed an increasing trend along with altitude, but the density in different sample plots was short of identical regularity, showing changes in different seasons and altitude grades had an important impact on small mammals such as rodents; 4) in species diversity and evenness index, there were obvious changes between the seven different sample plots, probably caused by frequent human interference in this area. Comparatively speaking, there was less human interference at high altitudes where vegetation was rich and had a high diversity and evenness index, and the boundary effect and community stability were obvious. Most ecological types have been seriously interfered with due to excessive assart at low altitudes with singular vegetation and low diversity and evenness index and poor community stability, showing an ecosystem with poor anti-reversion. If human interference can be reduced in those communities at high altitudes with low diversity and evenness index, the biological diversity in the communities will gradually recover to similar levels of other ecological areas.展开更多
Benthic macroinvertebrate communities in Spartina alterniflora zones in the Yangtze Estuary, in China, were investigated seasonally in 2005, and their structure and biodiversity were analyzed. Twenty-one species were ...Benthic macroinvertebrate communities in Spartina alterniflora zones in the Yangtze Estuary, in China, were investigated seasonally in 2005, and their structure and biodiversity were analyzed. Twenty-one species were identified, across four Classes; 10 species of Crustacea, five species of Polychaeta, five species of Gastropoda, and one species of Lamellibranchia. Dominant species included: Assiminea sp., Notomastus latericeus, Cerithidea largillierl, Gtauconome chinensi and Gammaridae sp. Functional groups were comprised of a phytophagous group and a detritivorous group. The average density of all benthic macroinvertebrates was 650.5 ±719.2 inds/m^2 in the survey area. The high value of the standard deviation of the average density was a result of abundant Assiminea sp. at Beihu tidal flats. The average density of macroinvertebrates from Beihu tidal flat, Chongming Dongtan to Jinshanwei tidal flat decreased gradually. There was significant difference between compositions and abundance of macroinvertebrates along the estuary gradient (P 〈 0.05). The density and biodiversity were highest in summer and lowest in winter. The mean biomass of macroinvertebrates was 20.8 ± 6.1 g/m^2. Biomass changed seasonally in the same way as density, with the change in biomass being: summer (Aug.) 〉autumn (Oct.) 〉spring (Apr.) 〉 winter (Dec.). A BIO-ENV analysis showed that the mean grain size of sediment, height of Spartina and salinity were the ma- jor factors which affected the structure of the macroinvertebrate community. Variations in the community structure were probably caused by the population dynamics of S. alterniflora along with the variation in sampling time and location.展开更多
Soil nematode communities were investigated in the Changbai Mountain in Broad-leaved Korean Pine forest, Korean Pine and spruce-fix mixed forest, Dark Coniferous forest, Erman's birch forest and Alpine tundra along d...Soil nematode communities were investigated in the Changbai Mountain in Broad-leaved Korean Pine forest, Korean Pine and spruce-fix mixed forest, Dark Coniferous forest, Erman's birch forest and Alpine tundra along different altitude gradients from 762 m to 2 200 m a.s.l. Soil animal samples were collected from the litter layer and the soil depth of 0-5 cm, 5-10 cm and 10-20 cm at each site in the spring of 2001 and 2002. In total 27 nematode families and 60 genera were observed. The dominant genera were Plectus Bastian and Tylenchus Bastian and most of them live in litter layer. The total number of soil nematode was significantly correlated with soil moisture (r=0.357; p〈0.01). Nematodes were classified in bacterivores, fungivores, plant parasites, omnivores-predators, and omnivores according to known feeding habitats or stoma and esophageal morphology. Species richness of fungivorous nematode was higher than others in different vegetation communities and soil depths. The total number of soil nematode and trophic groups varied significantly (o〈0.05) in response to different soil depths. The fungivore/bacterivore ratio (F/B) and the ratio of (fungivores + bacterivores)/plant-parasites (WI) also changed significantly (p〈0.05) in different soil depths. In conclusion, soil moisture is proved to be one of the most important variables affecting nematode density and trophic composition, and the altitude gradient does not significantly affect the ecological indices of soil nematode such as trophic diversity (TD) and the Shannon index (H').展开更多
The microbiotic crust study is among new focuses in investigating on the desertification control. Based on determination of algal crusts with different successive ages (4-, 8-, 17-, 34-, 42-year-old) and unconsolidate...The microbiotic crust study is among new focuses in investigating on the desertification control. Based on determination of algal crusts with different successive ages (4-, 8-, 17-, 34-, 42-year-old) and unconsolidated sand in the desert area, species composition and clustering analyses were carried out in this study. Results on successional orientation revealed that (1) the abundance of Cyanophyta, specially of Scytonema javanicum gradually decreased; (2) the abundance of Chlorophyta, Bacillariophyta and a species of Cyanophyta, Phormidium tenue increased; (3) the biodiversity increased gradually with the community succession; and (4) biomass of microalgae increased at the early stage, but decreased at the later stage due to the abundance of lichens and mosses. But, the speed of natural succession was so slow that the community-building species was still the first dominant species after 42 years, except that its dominant degree decreased just slightly. However, successive speed and trend were affected by water, vegetation coverage, terrain, time and soil physico-chemical properties as well, especially Mn content in the soil appeared to have a threshold effect.展开更多
The original strain HY-7 was isolated from the bauxite mine drainage(BMD) taken from a reservoir in Sanmenxia Mine,Henan Province,China.The optimum temperature and pH for the growth of strain HY-7 were 30 ℃ and 7.0...The original strain HY-7 was isolated from the bauxite mine drainage(BMD) taken from a reservoir in Sanmenxia Mine,Henan Province,China.The optimum temperature and pH for the growth of strain HY-7 were 30 ℃ and 7.0,respectively.The optimum UV radiating time was 20 s and the positive mutation rate was 23.0%.The growth curves show that strain HY-7 needs144 h to reach the stationary phase after its mutagenesis,which is 24 h earlier than that of the original strain.Sequence homology analysis indicated that this community consisted of mainly two branches:one sharing high homology with Paenibacillus stellifer and the other sharing high homology with Sporolactobacillus laevolacticus.The experimental results showed that the TiO2 grade of mtile concentrate increased from 78.21%to 91.80%and the recovery of TiO2 reached 95.24%after 7 d of bioleaching.The bio-desilication process can not only effectively improve the TiO2 grade of rutile concentrate but also meet the requirements of environmental protection.展开更多
[Objective] Surveys of phytoplankton were taken during the autumn of 2009 along Anhui-Jiangsu reaches of the Yangtze River to understand the species com- position and their spatial distributions. [Method] Phytoplankto...[Objective] Surveys of phytoplankton were taken during the autumn of 2009 along Anhui-Jiangsu reaches of the Yangtze River to understand the species com- position and their spatial distributions. [Method] Phytoplankton species, their quanti- ties, biomass and the Mcnaughton's dominance indices were all measured and their spatial distribution characteristics were determined using cluster analysis. [Result] There were 27 species, belonging to 5 phyla, namely Chlorophyta, Bacillariophyta, Cyanophyta, Euglenophyta and Cryptophyta, of phytoplankton collected and identified from the surveys. Results showed that Bacillariophyta was the predominant phyto- plankton with 16 species collected which accounted for 59.3% of the total species identified. The number of species collected belonging to Chlorophyta, Cyanophyta, Cryptophyta and Euglenophyta were 6, 2, 2, and 1, accounting for 22.2%, 7.4%, 7.4% and 3.7% of the total number identified, respectively. At the species level, the predominant species were Fragilaria crotonensis, Melosira italica, Navicula crypto- cephala, Cyclotella striata and Chroomonas acuta. Phytoplankton abundance was ranging from 5.68×10^4 to 7.08×10^4 cells/L with its average of 6.01×10^4 cells/L. Phyto- plankton biomass was ranging from 30.43 to 34.73 μg/L with its average of 32.46 μg/L. Compared with the previous reports, the number of phytoplankton species was decreased but its abundance and biomass was increased along the Jiangsu reach of the Yangtze River. However, Bacillariophyta species were still the predominant species and the phytoplankton community structure had not significantly changed from the previous studies. [Conclusion] These results might be explained as that the water quality in the Yangtze River was deteriorated but had not come to the worst. The results of similarity analysis gave two clusters of phytoplankton community as Nanjing, Wuhu and Jiangyin sampling sites were clustered into one group and Tongling and Anqing were clustered into another group.展开更多
[Objective] The aim was to research macrobenthos community structure and its relations with environmental factors in three reservoirs. [Method] Benthic diversity and water quality of Yuqiao Reservoir, Beidagang Reserv...[Objective] The aim was to research macrobenthos community structure and its relations with environmental factors in three reservoirs. [Method] Benthic diversity and water quality of Yuqiao Reservoir, Beidagang Reservoir and Er- wangzhuang Reservoir were investigated in May, July and September 2011, respec- tively. []Result] Of all the 14 sampling sites, 22 species of macroinvertebrates were collected, totally, which included 6 species of aquatic insects, 7 species of Mollusca, 2 species of Crustacean, 7 species of Oligochaeta. The species of macroinverte- brates during May, July and September in Yuqiao reservoir were 11, 8 and 10, respectively. The dominant species were Chironomus sinicus, and Monopylephorus limosus. The average density and biomass of the macrobenthos were 132.43 ind./m2 and 50.09 g/m2. Yuqiao reservoir was mostly at status of premediate polluted, according to the Margalef index, Shannon index and Pielou index. The species of during May, July and September in Beidagang reservoir were 5 5 and 5, respectively. The dominant species were Chironomus sinicus, Penaeus vannamei. The average density and biomass of the macrobenthos were 218 ind./m2 and 2.70 g/m2. Beidagang reservoir was mostly at status of heavy polluted, accord- ing to the Margalef index, Shannon-wiener index, Pielou index. The species of during May, July and September in Erwangzhuang reservoir were 3, 5 and 5, respectively. The dominant species were Monopylephorus limosus, Chi- ronomus sinicus. The average density and biomass of the macrobenthos were 104 ind/m2 and 0.20 g/m2. Erwangzhuang reservoir was mostly at status of intermediate polluted, according to the Margalef index, Shannon index, Pielou index. The density of zoobenthos in Yuqiao Reservoir was positively significantly correlated with TP (P〉 0.05), negatively correlated with NH4+-N (P〉0.05), positively correlated with COD (P〈 0.01), not significantly correlated with other environment factors (P〉0.05). There was no significant correlation between biomass of zoobenthos and environment factors. The density of zoobenthos in Beidagang Reservoir with NH4+-N was positively signif- icantly correlated (P〈0.05), not significantly correlated with other aquatic environment factors (P〉0.05). There was no significant correlation between biomass of zoobenthos and environment factors. The density of zoobenthos in Erwangzhuang Reser- voir with NO3-N was positively significantly correlated (P〈0.05), not significant cor- related with other aquatic environment factors (F〉0.05). The biomass of zoobenthos in Erwangzhuang Reservoir with NH4+-N was positively significantly correlated (P〈 0.05), not significant correlated with other aquatic environment factors (P〉0.05). [Conclusion] It is indicated that this three reservoirs were all eutrophied and polluted, and the eurephication extent change of water in three reservoirs is as follows: Beidagang reservoir〉Erwangzhuang reservoir〉Yuqiao reservoir.展开更多
The survey on bird communities was conducted by the belt-style method in six different sample plots in the Honghua抏rji Forests area in the northern Inner Mongolia in June 2001 and totally 28 bird species were recorde...The survey on bird communities was conducted by the belt-style method in six different sample plots in the Honghua抏rji Forests area in the northern Inner Mongolia in June 2001 and totally 28 bird species were recorded. Vegetation investigation was carried out in five 10 m×10 m quadrats at each plot. The asymptotic regression function formulae were adopted to identify the relationships between the vegetation coverage and the numbers of bird species and individuals. The analytical results showed that the changes of species number and density of bird as well as the formation of bird communities follow the changes of forest type and the total foliage. Both the number of bird species and their density decreased with the de-crease of total foliage. The similarity of bird community was very low at the breeding time. In the same classification of cluster, no similarity was higher than 0.65, which indicated that the composition of species had a great difference between all the bird communities. The bird breeding density was closely related to forest growth stage. From the bare grassland ecosystem to cli-max ecosystem, the density of bird species showed a gradually increasing trend.展开更多
Mollusks, arthropods and chordates which were visible to the naked eye were observed and collected in Dongbei and Shuijiang caves of Libo county five times between February and July from 2002 to 2005. Four hundred and...Mollusks, arthropods and chordates which were visible to the naked eye were observed and collected in Dongbei and Shuijiang caves of Libo county five times between February and July from 2002 to 2005. Four hundred and forty samples from Dongbei Cave were classified into three phyla, five classes, 10 orders, 20 families and 39 species or groups of species. Four hundred and ninety-eight samples from Shuijiang Cave were classified into three phyla, six classes, 11 orders, 20 families and 25 species or groups of species. Six animal communities were identified in the light belt of the two caves according to their species types and numbers of individuals in the light belt of two cave. The communities which have the highest values of species richness community diversity, maximum diversity, evenness, dominance and community similarity are respectively: B (4. 1059), H (2.4716), B (3.3322), E (0.9042), C (0.3442) and A - C (0. 5251). The community diversity and correlation of environmental factors were also studied. The temperature, humidity, content of CO2 and N2, content of organic matter and some inorganic salts in soil were analyzed by Pearson correlation. The results showed that the content of organic matter in soil is positively related to species number, species richness and maximum community diversity, with correlations of 0. 885, 0.909, 0. 868 respectively (two-tailed significance test, P ≤ 0. 05), and significantly positively related to diversity, with the coefficient of 0.611, (two-tailed significance test, P ≤ 0. 1). This suggests that the content of organic matter in soil is one of the important elements influencing the community variation of cave animals. Outside the cave, temperature and humidity are important factors impacting on community diver- sity. As the temperature in all seasons in the cave is stable and the humidity is always high (above 90% ), the temperature and humidity have weak correlation to community diversity in caves.展开更多
[ Objective ] The paper was to study the community structure and diversity of ladybugs in Baihualing of Gaoligong Mountain, and fill gaps in research about ladybugs in this region. [ Method] Using sampling plot invest...[ Objective ] The paper was to study the community structure and diversity of ladybugs in Baihualing of Gaoligong Mountain, and fill gaps in research about ladybugs in this region. [ Method] Using sampling plot investigation method, the species composition and population structure of ladybugs in Baihualing of Gaoligong Mountain were surveyed. [ Result ] A total of 3 218 ladybugs specimens had been collected, belonging to 5 subfamilies, 20 genera, 56 species. Two spe- cies were new records for Yurman Province. The species and number of Coccinellinae were the greatest, followed by Epilachninae and Aspidimerinae, while Coceid- ulinae and Scymninae were the least. The dominant species were Coccinella septempunctata L., Harmonia eucharis ( Mulsant ) and Afissula hydrangeae Pang et Man. [ Conclusion] The study laid foundation for further study on ladybugs in Baihualing of Gaoligong Mountain.展开更多
To know the bacterial communities structure in Babylonia areolata culture systems and to research and optimize the management pattem of Babylonia areola-ta culture systems of the pond mulched plastic film and sand in ...To know the bacterial communities structure in Babylonia areolata culture systems and to research and optimize the management pattem of Babylonia areola-ta culture systems of the pond mulched plastic film and sand in bottom, the bacte- rial communities in Babylonia areolata culture systems of the sub-tidal zone and the pond mulched plastic film and sand in bottom were analyzed at molecular level by adopting the denaturing gradient gel electrophoresis (DGGE). The results indicated that the dominant bacterial communities in Babylonia areolata culture systems of the sub-tidal zone and the pond mulched plastic film and sand in bottom, which were built on the basis of the seawater in East-island of Zhanjiang, included Proteobac- teda Chloroflexi, Cyanobacteria and Actinobacteria. The dominant bacterial groups in the above pond culture system were Garnmaproteobacteria, Alphaproteobacteria, Deltaprotecbacteda, Epsilonproteobacteda, Anaerolineae, Cyanobacteria and Acti- nobacteda. The dominant bacterial communities in the subtidal zone culture system were Gammaprotecbacteda, Alphaproteobacteria, Deltaproteobacteria, Anaerolineae and Cyanobacteda, and there were less Epsilonproteobacteria and Actinobacteria in the culture system. The higher diversity was detected in the above two culture sys- tems. The results of unweighted pair group method with arithmetic average (UPG- MA) showed that the bacterial communities of the sediment samples S1 and S2 in the above two culture systems were a cluster, the similarity of bacterial communities was 54.5%. The bacterial communities of seawater samples S3 and S4 in the above culture systems were in clusters, and the similarity of the bacterial communi- ties was 84.0%. The results showed that the microorganism ecological level in the Babylonia areolata culture systems of the pond mulched plastic film and sand in bottom could be similar to the sub-tidal zone culture systems through changing the pond seawater and monitoring the microbial population.展开更多
Studies showed that complexation of polyphenols with milk allergens reduced their immunogenic potential.However,the relationship between structures of polyphenols and their hypoallergenic effects on milk allergens in ...Studies showed that complexation of polyphenols with milk allergens reduced their immunogenic potential.However,the relationship between structures of polyphenols and their hypoallergenic effects on milk allergens in association with physiological and conformational changes of the complexes remain unclear.In this study,polyphenols from eight botanical sources were extracted to prepare non-covalent complexes withβ-lactoglobulin(β-LG),a major allergen in milk.The dominant phenolic compounds bound toβ-LG with a diminished allergenicity were identified to investigate their respective role on the structural and allergenic properties ofβ-LG.Extracts from Vaccinium fruits and black soybeans were found to have great inhibitory effects on the IgE-and IgG-binding abilities ofβ-LG.Among the fourteen structure-related phenolic compounds,flavonoids and tannins with larger MWs and multi-hydroxyl substituents,notably rutin,EGCG,and ellagitannins were more potent to elicit changes on the conformational structures ofβ-LG to decrease the allergenicity of complexedβ-LG.Correlation analysis further demonstrated that a destabilized secondary structure and protein depolymerization caused by polyphenol-binding were closely related to the allergenicity property of formed complexes.This study provides insights into the understanding of structure-allergenicity relationship ofβ-LG-polyphenol interactions and would benefit the development of polyphenol-fortified matrices with hypoallergenic potential.展开更多
Biolog, 16S rRNA gene denaturing gradient gel electrophoresis (DGGE), and phospholipid fatty acid (PLFA) analyses were used to assess soil microbial community characteristics in a chronosequence of tea garden syst...Biolog, 16S rRNA gene denaturing gradient gel electrophoresis (DGGE), and phospholipid fatty acid (PLFA) analyses were used to assess soil microbial community characteristics in a chronosequence of tea garden systems (8-, 50-, and 90- year-old tea gardens), an adjacent wasteland, and a 90-year-old forest. Biolog analysis showed that the average well color development (AWCD) of all carbon sources and the functional diversity based on the Shannon index decreased (P 〈 0.05) in the following order: wasteland 〉 forest 〉 tea garden. For the DCCE analysis, the genetic diversity based on the Shannon index was significantly lower in the tea garden soils than in the wasteland. However, compared to the 90-year-old forest, the tea garden soils showed significantly higher genetic diversity. PLFA analysis showed that the ratio of Gram positive bacteria to Cram negative bacteria was significantly higher in the tea garden soils than in the wasteland, and the highest value was found in the 90-year-old forest. Both the fungal PLFA and the ratio of fungi to bacteria were significantly higher in the three tea garden soils than in the wasteland and forest, indicating that fungal PLFA was significantly affected by land-use change. Based on cluster analysis of the soil microbial community structure, all three analytical methods showed that land-use change had a greater effect on soil microbial community structure than tea garden age.展开更多
Effects of suction dredging on water quality and zooplankton community structure in a shallow of eutrophic lake, were evaluated. The results showed that a decreasing trend for levels of phosphorus, organic matter, tot...Effects of suction dredging on water quality and zooplankton community structure in a shallow of eutrophic lake, were evaluated. The results showed that a decreasing trend for levels of phosphorus, organic matter, total suspended solids, Chlorophyll a and Secchi transparency in the water column was found, while levels of water depth, electrical conductivity, total dissolved solids and NO3^--N concentration increased markedly post-dredging. The effects of dredging on dissolved oxygen, pH value and temperature were almost negligible. The zooplankton community structure responded rapidly to the environmental changes caused mainly by dredging. As a result, the abundance of rotifers decreased, while the density of zooplanktonic crustaceans increased markedly. The representative taxa were Brachionus angularis, B. budapestinensis, B. diversicornis, Synchaeta spp. and Neodiaptomus schmackeri. A distinct relationship between zooplankton taxa composition and their environment, unraveled by a redundancy analysis, indicating that the measured environment contributed to the variations in the zooplankton community structure to some extent. The first four synthetic environmental variables explained 51.7% of the taxonomic structure. Therefore, with the reduction of internal nutrient load and a shift in dominance by less eutrophic species, it inferred that dredging might be one of effective measures for environmental improvements of such lakes.展开更多
Increasing levels of pollution within water bodies can cause eutrophication and an associated rapid growth in and reproduction of phytoplankton. Although most frequently occurring in bodies of water such as lakes and ...Increasing levels of pollution within water bodies can cause eutrophication and an associated rapid growth in and reproduction of phytoplankton. Although most frequently occurring in bodies of water such as lakes and dams, in recent years an increasing number of river systems in China have suffered serious algal blooms. The community structure of phytoplankton may differ, however, dependent on the hydrodynamic conditions and nutrient levels within the water body. The field investigation results obtained from a stagnant river in Suzhou City and Taihu Lake, China, showed that in water with higher concentrations of nitrogen and phosphorus, Chlorophyta became the predominant species and in water with lower concentrations of nitrogen and phosphorus, Cyanobacteria became the predominant species. Growth experiments with competitive species, Microcystis aeruginosa Kutz and Scenedesmus quadricauda (Turp.), were conducted at three different nutrient levels. The biomass of algae in pure and mixed cultures was measured under conditions of different N/P ratios at oligotrophic, eutrophic and hypertrophic nutrient levels. The results indicated that the most suitable state for the growth and reproduction of M. aeruginosa and S. quadricauda were eutrophic conditions in both pure and mixed cultures. Under competition, however, the lower medium nutrient levels favoured M. aeruginosa, while the higher medium nutrient levels better suited S. quadricauda. Under similar hydrodynamic conditions, the community structure of phytoplankton in the water body was determined by the dominant species in competition for nutrients.展开更多
On the basis of data collected in the summer of 2006 from 27 sampling stations in the Changjiang Estuary and its adjacent waters, the ecological characteristics of macrobenthos and the relationship between the macrobe...On the basis of data collected in the summer of 2006 from 27 sampling stations in the Changjiang Estuary and its adjacent waters, the ecological characteristics of macrobenthos and the relationship between the macrobenthos and the environmental factors were studied using hierarchical cluster and non-metric multidimensional scaling (MDS). The biomass, abundance, Shannon -Wiener's and Margalef' s indices of the macrobenthos were presented. The results showed that a total of 253 macrobenthic species were found in the research region, and most of them belong to mollusks and polychaetes. The dominant species were Cossurella dimorpha, Eocylichna cylindrella, Episiphon kiaochowwanense, Nassarius semiplicatus, Ocstergrenia variabilis and Sternaspis scutata. The average abundance of the macrobenthos was (313. 15 ±233.4) ind./m^2, and the average biomass was ( 15. 2 ± 11.2 )g/m^2. The distribution patterns of the abundance and biomass of the macrobenthos were similar. The abundance and biomass in the area close to the estuary were lower than those from the area more distant to the estuary; the central part of the research region had higher abundance and biomass than other parts of the research region. In accordance with the results, four macrobenthic communities with distinct spatial differences were identified. The low abundance and biomass in the area close to the estuary should be caused by the high sedimentation rate. The statistical analysis indicated that the depth is the most important factor affecting the distribution of macrobenthos.展开更多
Here, using the Scale-Symmetric Theory (SST) we explain the cosmological tension and the origin of the largest cosmic structures. We show that a change in value of strong coupling constant for cold baryonic matter lea...Here, using the Scale-Symmetric Theory (SST) we explain the cosmological tension and the origin of the largest cosmic structures. We show that a change in value of strong coupling constant for cold baryonic matter leads to the disagreement in the galaxy clustering amplitude, quantified by the parameter S8. Within the same model we described the Hubble tension. We described also the mechanism that transforms the gravitational collapse into an explosion—it concerns the dynamics of virtual fields that lead to dark energy. Our calculations concern the Type Ia supernovae and the core-collapse supernovae. We calculated the quantized masses of the progenitors of supernovae, emitted total energy during explosion, and we calculated how much of the released energy was transferred to neutrinos. Value of the speed of sound in the strongly interacting matter measured at the LHC confirms that presented here model is correct. Our calculations show that the Universe is cyclic.展开更多
基金supported in part by Award 2121063 from National Science Foundation(to YM)AG66986 from the National Institutes of Health(to MSW).
文摘γ-Secretase,called“the proteasome of the membrane,”is a membrane-embedded protease complex that cleaves 150+peptide substrates with central roles in biology and medicine,including amyloid precursor protein and the Notch family of cell-surface receptors.Mutations inγ-secretase and amyloid precursor protein lead to early-onset familial Alzheimer’s disease.γ-Secretase has thus served as a critical drug target for treating familial Alzheimer’s disease and the more common late-onset Alzheimer’s disease as well.However,critical gaps remain in understanding the mechanisms of processive proteolysis of substrates,the effects of familial Alzheimer’s disease mutations,and allosteric modulation of substrate cleavage byγ-secretase.In this review,we focus on recent studies of structural dynamic mechanisms ofγ-secretase.Different mechanisms,including the“Fit-Stay-Trim,”“Sliding-Unwinding,”and“Tilting-Unwinding,”have been proposed for substrate proteolysis of amyloid precursor protein byγ-secretase based on all-atom molecular dynamics simulations.While an incorrect registry of the Notch1 substrate was identified in the cryo-electron microscopy structure of Notch1-boundγ-secretase,molecular dynamics simulations on a resolved model of Notch1-boundγ-secretase that was reconstructed using the amyloid precursor protein-boundγ-secretase as a template successfully capturedγ-secretase activation for proper cleavages of both wildtype and mutant Notch,being consistent with biochemical experimental findings.The approach could be potentially applied to decipher the processing mechanisms of various substrates byγ-secretase.In addition,controversy over the effects of familial Alzheimer’s disease mutations,particularly the issue of whether they stabilize or destabilizeγ-secretase-substrate complexes,is discussed.Finally,an outlook is provided for future studies ofγ-secretase,including pathways of substrate binding and product release,effects of modulators on familial Alzheimer’s disease mutations of theγ-secretase-substrate complexes.Comprehensive understanding of the functional mechanisms ofγ-secretase will greatly facilitate the rational design of effective drug molecules for treating familial Alzheimer’s disease and perhaps Alzheimer’s disease in general.
基金funded by the Guangxi Natural Science Foundation Program (2022GXNSFAA035583 and 2020GXNSFAA159108)National Natural Science Foundation of China (32060305)+2 种基金Foundation of Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection (Guangxi Normal University)Ministry of Education, China (ERESEP 2021Z06)Chinese Forest Biodiversity Monitoring Network
文摘Here,we characterize the temporal and spatial dynamics of forest community structure and species diversity in a subtropical evergreen broad-leaved forest in China.We found that community structure in this forest changed over a 15-year period.Specifically,renewal and death of common species was large,with the renewal of individuals mainly concentrated within a few populations,especially those of Aidia canthioides and Cryptocarya concinna.The numbers of individual deaths for common species were concentrated in the small and mid-diameter level.The spatial distribution of community species diversity fluctuated in each monitoring period,showing a more dispersed diversity after the 15-year study period,and the coefficient of variation on quadrats increased.In 2010,the death and renewal of the community and the spatial variation of species diversity were different compared to other survey years.Extreme weather may have affected species regeneration and community stability in our subtropical monsoon evergreen broad-leaved forests.Our findings suggest that strengthening the monitoring and management of the forest community will help better understand the long-and short-term causes of dynamic fluctuations of community structure and species diversity,and reveal the factors that drive changes in community structure.
文摘Aim: This paper aims to evaluate disparities of type 2 diabetes structured health education programmes that is utilised within the communities. Design: systematic review, (a type of secondary research design) aiming to summarize the results of prior primary research studies on available evidence Community type 2 diabetes structured education (CT2DSHE). Methods: Research question: Type 2 diabetic structured health education within a community how effective is it? Qualitative Systematic review, defined as a way to get reliable and objective picture of current available evidence on the specific topic—(CT2DSHE), (Denscombe, 2021) through reflexivity synthesis of available data as an example. This is valuable in time constraints such as project assignments that must be met within specific time and also to bring together available evidence together [1]. Results: This review has shown that CT2DSHE is effective with seven out of the eleven authors supporting, three authors against and one was neutral, further showed that knowledge and skills acquired can last longer with patient activation improved among T2DM patients ideal for sustaining their self-management of T2DM. Conclusion: This research provides suggestive answers to the research question: “Type 2 diabetic structured health education within a community how effective is it?”, This has demonstrated CT2DSHE effectiveness in knowledge acquisition and improving T2DM awareness among T2DM patients, whilst evidencing long effects beyond the study times of 3 - 9 months period in relation to patient activation. Also Identified diabetes education self-management on newly diagnosed (DESMOND) patient as CT2DSHE program for recommendation. Patient or Public Contribution: This work aspires to contribute to CT2DSHE in these areas;Influencing policy decision-making for community diabetes care within the UK and world at large., Contributing to already vast knowledge on diabetes self-management and reasons why?, Influencing educators on how CT2DSHEP are designed, delivered by putting the patient at the Centre and bringing different perspectives on CT2DSHEP in one place that is serving users time of having to consult several resources especially busy clinicians [2] [3].
基金National Natural Science Foundation of China(30070679)the Natural Science Foundation of Hubei Province(2004ABA138)+1 种基金the Key Technology R&D Programme Foundation of Hubei Province(2002AA301C43)the Hubei Health Bureau Research Programme Foundation(NX200427)
文摘Five years' (2000-2004) continuous study has been carried out on small mammals such as rodents in seven different sample plots, at three different altitudes and in six different ecological environment types in the eastern part of the Wuling Mountains, south bank of the Three Gorges of Yangtze River in Hubei. A total of 29 297 rat clamps/times were placed and 2271 small mammals such as rodents were captured, and 26 small mammals were captured by other means. All the small mammals captured belonged to 8 families 19 genera and 24 species, of which rodentia accounted for 70.83% and insectivora 29.17%. Through analysis of the data, the results showed that: 1 ) although the species richness had a trend of increasing along different sample plots as altitude increased from south to north, quite a few species showed a wide habitat range in a vertical distribution ( 15 species were dispersed over three zones and two species over two zones) , indicating a strong adaptability of small mammals such as rOdents at lower altitudes in most areas and comparatively less vertical span of entire mountains; 2) whether in seven different sample plots or six different ecological types, Apodemus agrarius and Rattus norvegicus were dominant species below 1200m, and Anourosorex squamipes, Niviventer confucianus and Apodemus draco were dominant above altitudes of 1300m, however, in quantity they were short of identical regularity, meaning they did not increase as the altitude did, or decrease as the ecological areas changed; 3)the density in winter was obviously greater than that in spring, and the distribution showed an increasing trend along with altitude, but the density in different sample plots was short of identical regularity, showing changes in different seasons and altitude grades had an important impact on small mammals such as rodents; 4) in species diversity and evenness index, there were obvious changes between the seven different sample plots, probably caused by frequent human interference in this area. Comparatively speaking, there was less human interference at high altitudes where vegetation was rich and had a high diversity and evenness index, and the boundary effect and community stability were obvious. Most ecological types have been seriously interfered with due to excessive assart at low altitudes with singular vegetation and low diversity and evenness index and poor community stability, showing an ecosystem with poor anti-reversion. If human interference can be reduced in those communities at high altitudes with low diversity and evenness index, the biological diversity in the communities will gradually recover to similar levels of other ecological areas.
文摘Benthic macroinvertebrate communities in Spartina alterniflora zones in the Yangtze Estuary, in China, were investigated seasonally in 2005, and their structure and biodiversity were analyzed. Twenty-one species were identified, across four Classes; 10 species of Crustacea, five species of Polychaeta, five species of Gastropoda, and one species of Lamellibranchia. Dominant species included: Assiminea sp., Notomastus latericeus, Cerithidea largillierl, Gtauconome chinensi and Gammaridae sp. Functional groups were comprised of a phytophagous group and a detritivorous group. The average density of all benthic macroinvertebrates was 650.5 ±719.2 inds/m^2 in the survey area. The high value of the standard deviation of the average density was a result of abundant Assiminea sp. at Beihu tidal flats. The average density of macroinvertebrates from Beihu tidal flat, Chongming Dongtan to Jinshanwei tidal flat decreased gradually. There was significant difference between compositions and abundance of macroinvertebrates along the estuary gradient (P 〈 0.05). The density and biodiversity were highest in summer and lowest in winter. The mean biomass of macroinvertebrates was 20.8 ± 6.1 g/m^2. Biomass changed seasonally in the same way as density, with the change in biomass being: summer (Aug.) 〉autumn (Oct.) 〉spring (Apr.) 〉 winter (Dec.). A BIO-ENV analysis showed that the mean grain size of sediment, height of Spartina and salinity were the ma- jor factors which affected the structure of the macroinvertebrate community. Variations in the community structure were probably caused by the population dynamics of S. alterniflora along with the variation in sampling time and location.
基金funded by the National Science Foundation of China and project(Grant No.G1999043407)supported by the National Key Basic Research Special Funds(Nos.30170744and G1999043407)
文摘Soil nematode communities were investigated in the Changbai Mountain in Broad-leaved Korean Pine forest, Korean Pine and spruce-fix mixed forest, Dark Coniferous forest, Erman's birch forest and Alpine tundra along different altitude gradients from 762 m to 2 200 m a.s.l. Soil animal samples were collected from the litter layer and the soil depth of 0-5 cm, 5-10 cm and 10-20 cm at each site in the spring of 2001 and 2002. In total 27 nematode families and 60 genera were observed. The dominant genera were Plectus Bastian and Tylenchus Bastian and most of them live in litter layer. The total number of soil nematode was significantly correlated with soil moisture (r=0.357; p〈0.01). Nematodes were classified in bacterivores, fungivores, plant parasites, omnivores-predators, and omnivores according to known feeding habitats or stoma and esophageal morphology. Species richness of fungivorous nematode was higher than others in different vegetation communities and soil depths. The total number of soil nematode and trophic groups varied significantly (o〈0.05) in response to different soil depths. The fungivore/bacterivore ratio (F/B) and the ratio of (fungivores + bacterivores)/plant-parasites (WI) also changed significantly (p〈0.05) in different soil depths. In conclusion, soil moisture is proved to be one of the most important variables affecting nematode density and trophic composition, and the altitude gradient does not significantly affect the ecological indices of soil nematode such as trophic diversity (TD) and the Shannon index (H').
文摘The microbiotic crust study is among new focuses in investigating on the desertification control. Based on determination of algal crusts with different successive ages (4-, 8-, 17-, 34-, 42-year-old) and unconsolidated sand in the desert area, species composition and clustering analyses were carried out in this study. Results on successional orientation revealed that (1) the abundance of Cyanophyta, specially of Scytonema javanicum gradually decreased; (2) the abundance of Chlorophyta, Bacillariophyta and a species of Cyanophyta, Phormidium tenue increased; (3) the biodiversity increased gradually with the community succession; and (4) biomass of microalgae increased at the early stage, but decreased at the later stage due to the abundance of lichens and mosses. But, the speed of natural succession was so slow that the community-building species was still the first dominant species after 42 years, except that its dominant degree decreased just slightly. However, successive speed and trend were affected by water, vegetation coverage, terrain, time and soil physico-chemical properties as well, especially Mn content in the soil appeared to have a threshold effect.
基金Project(2011-622-40)supported by the Mineral Exploration Foundation of Henan Province,ChinaProject(51104189)supported by the National Natural Science Foundation of ChinaProject(2013M531814)supported by the 53rd China Postdoctoral Science Foundation
文摘The original strain HY-7 was isolated from the bauxite mine drainage(BMD) taken from a reservoir in Sanmenxia Mine,Henan Province,China.The optimum temperature and pH for the growth of strain HY-7 were 30 ℃ and 7.0,respectively.The optimum UV radiating time was 20 s and the positive mutation rate was 23.0%.The growth curves show that strain HY-7 needs144 h to reach the stationary phase after its mutagenesis,which is 24 h earlier than that of the original strain.Sequence homology analysis indicated that this community consisted of mainly two branches:one sharing high homology with Paenibacillus stellifer and the other sharing high homology with Sporolactobacillus laevolacticus.The experimental results showed that the TiO2 grade of mtile concentrate increased from 78.21%to 91.80%and the recovery of TiO2 reached 95.24%after 7 d of bioleaching.The bio-desilication process can not only effectively improve the TiO2 grade of rutile concentrate but also meet the requirements of environmental protection.
基金Supported by Fishery Germplasm Conservation Project of the Ministry of Agriculture(MOA)(No.6115048)State Specific Project on Fundamental Scientific Research Financed to Public Institutes(No.2009JBFB10)~~
文摘[Objective] Surveys of phytoplankton were taken during the autumn of 2009 along Anhui-Jiangsu reaches of the Yangtze River to understand the species com- position and their spatial distributions. [Method] Phytoplankton species, their quanti- ties, biomass and the Mcnaughton's dominance indices were all measured and their spatial distribution characteristics were determined using cluster analysis. [Result] There were 27 species, belonging to 5 phyla, namely Chlorophyta, Bacillariophyta, Cyanophyta, Euglenophyta and Cryptophyta, of phytoplankton collected and identified from the surveys. Results showed that Bacillariophyta was the predominant phyto- plankton with 16 species collected which accounted for 59.3% of the total species identified. The number of species collected belonging to Chlorophyta, Cyanophyta, Cryptophyta and Euglenophyta were 6, 2, 2, and 1, accounting for 22.2%, 7.4%, 7.4% and 3.7% of the total number identified, respectively. At the species level, the predominant species were Fragilaria crotonensis, Melosira italica, Navicula crypto- cephala, Cyclotella striata and Chroomonas acuta. Phytoplankton abundance was ranging from 5.68×10^4 to 7.08×10^4 cells/L with its average of 6.01×10^4 cells/L. Phyto- plankton biomass was ranging from 30.43 to 34.73 μg/L with its average of 32.46 μg/L. Compared with the previous reports, the number of phytoplankton species was decreased but its abundance and biomass was increased along the Jiangsu reach of the Yangtze River. However, Bacillariophyta species were still the predominant species and the phytoplankton community structure had not significantly changed from the previous studies. [Conclusion] These results might be explained as that the water quality in the Yangtze River was deteriorated but had not come to the worst. The results of similarity analysis gave two clusters of phytoplankton community as Nanjing, Wuhu and Jiangyin sampling sites were clustered into one group and Tongling and Anqing were clustered into another group.
基金Supported by Tianjin Aquatic Species Diveristy Surveying,Monitoring and Evaluation Proejct~~
文摘[Objective] The aim was to research macrobenthos community structure and its relations with environmental factors in three reservoirs. [Method] Benthic diversity and water quality of Yuqiao Reservoir, Beidagang Reservoir and Er- wangzhuang Reservoir were investigated in May, July and September 2011, respec- tively. []Result] Of all the 14 sampling sites, 22 species of macroinvertebrates were collected, totally, which included 6 species of aquatic insects, 7 species of Mollusca, 2 species of Crustacean, 7 species of Oligochaeta. The species of macroinverte- brates during May, July and September in Yuqiao reservoir were 11, 8 and 10, respectively. The dominant species were Chironomus sinicus, and Monopylephorus limosus. The average density and biomass of the macrobenthos were 132.43 ind./m2 and 50.09 g/m2. Yuqiao reservoir was mostly at status of premediate polluted, according to the Margalef index, Shannon index and Pielou index. The species of during May, July and September in Beidagang reservoir were 5 5 and 5, respectively. The dominant species were Chironomus sinicus, Penaeus vannamei. The average density and biomass of the macrobenthos were 218 ind./m2 and 2.70 g/m2. Beidagang reservoir was mostly at status of heavy polluted, accord- ing to the Margalef index, Shannon-wiener index, Pielou index. The species of during May, July and September in Erwangzhuang reservoir were 3, 5 and 5, respectively. The dominant species were Monopylephorus limosus, Chi- ronomus sinicus. The average density and biomass of the macrobenthos were 104 ind/m2 and 0.20 g/m2. Erwangzhuang reservoir was mostly at status of intermediate polluted, according to the Margalef index, Shannon index, Pielou index. The density of zoobenthos in Yuqiao Reservoir was positively significantly correlated with TP (P〉 0.05), negatively correlated with NH4+-N (P〉0.05), positively correlated with COD (P〈 0.01), not significantly correlated with other environment factors (P〉0.05). There was no significant correlation between biomass of zoobenthos and environment factors. The density of zoobenthos in Beidagang Reservoir with NH4+-N was positively signif- icantly correlated (P〈0.05), not significantly correlated with other aquatic environment factors (P〉0.05). There was no significant correlation between biomass of zoobenthos and environment factors. The density of zoobenthos in Erwangzhuang Reser- voir with NO3-N was positively significantly correlated (P〈0.05), not significant cor- related with other aquatic environment factors (F〉0.05). The biomass of zoobenthos in Erwangzhuang Reservoir with NH4+-N was positively significantly correlated (P〈 0.05), not significant correlated with other aquatic environment factors (P〉0.05). [Conclusion] It is indicated that this three reservoirs were all eutrophied and polluted, and the eurephication extent change of water in three reservoirs is as follows: Beidagang reservoir〉Erwangzhuang reservoir〉Yuqiao reservoir.
文摘The survey on bird communities was conducted by the belt-style method in six different sample plots in the Honghua抏rji Forests area in the northern Inner Mongolia in June 2001 and totally 28 bird species were recorded. Vegetation investigation was carried out in five 10 m×10 m quadrats at each plot. The asymptotic regression function formulae were adopted to identify the relationships between the vegetation coverage and the numbers of bird species and individuals. The analytical results showed that the changes of species number and density of bird as well as the formation of bird communities follow the changes of forest type and the total foliage. Both the number of bird species and their density decreased with the de-crease of total foliage. The similarity of bird community was very low at the breeding time. In the same classification of cluster, no similarity was higher than 0.65, which indicated that the composition of species had a great difference between all the bird communities. The bird breeding density was closely related to forest growth stage. From the bare grassland ecosystem to cli-max ecosystem, the density of bird species showed a gradually increasing trend.
文摘Mollusks, arthropods and chordates which were visible to the naked eye were observed and collected in Dongbei and Shuijiang caves of Libo county five times between February and July from 2002 to 2005. Four hundred and forty samples from Dongbei Cave were classified into three phyla, five classes, 10 orders, 20 families and 39 species or groups of species. Four hundred and ninety-eight samples from Shuijiang Cave were classified into three phyla, six classes, 11 orders, 20 families and 25 species or groups of species. Six animal communities were identified in the light belt of the two caves according to their species types and numbers of individuals in the light belt of two cave. The communities which have the highest values of species richness community diversity, maximum diversity, evenness, dominance and community similarity are respectively: B (4. 1059), H (2.4716), B (3.3322), E (0.9042), C (0.3442) and A - C (0. 5251). The community diversity and correlation of environmental factors were also studied. The temperature, humidity, content of CO2 and N2, content of organic matter and some inorganic salts in soil were analyzed by Pearson correlation. The results showed that the content of organic matter in soil is positively related to species number, species richness and maximum community diversity, with correlations of 0. 885, 0.909, 0. 868 respectively (two-tailed significance test, P ≤ 0. 05), and significantly positively related to diversity, with the coefficient of 0.611, (two-tailed significance test, P ≤ 0. 1). This suggests that the content of organic matter in soil is one of the important elements influencing the community variation of cave animals. Outside the cave, temperature and humidity are important factors impacting on community diver- sity. As the temperature in all seasons in the cave is stable and the humidity is always high (above 90% ), the temperature and humidity have weak correlation to community diversity in caves.
基金Supported by Sub-projects of Standardized Sorting,Integration and Sharing of Biological Specimens in Nature Reserve~~
文摘[ Objective ] The paper was to study the community structure and diversity of ladybugs in Baihualing of Gaoligong Mountain, and fill gaps in research about ladybugs in this region. [ Method] Using sampling plot investigation method, the species composition and population structure of ladybugs in Baihualing of Gaoligong Mountain were surveyed. [ Result ] A total of 3 218 ladybugs specimens had been collected, belonging to 5 subfamilies, 20 genera, 56 species. Two spe- cies were new records for Yurman Province. The species and number of Coccinellinae were the greatest, followed by Epilachninae and Aspidimerinae, while Coceid- ulinae and Scymninae were the least. The dominant species were Coccinella septempunctata L., Harmonia eucharis ( Mulsant ) and Afissula hydrangeae Pang et Man. [ Conclusion] The study laid foundation for further study on ladybugs in Baihualing of Gaoligong Mountain.
基金Supported by the Special Program of Scientific and Technological Promotion of Fisheries in Guangdong(A201101I01,A201208E01)the Guangdong Scientific and Technological Planning Program(2012B020415006)~~
文摘To know the bacterial communities structure in Babylonia areolata culture systems and to research and optimize the management pattem of Babylonia areola-ta culture systems of the pond mulched plastic film and sand in bottom, the bacte- rial communities in Babylonia areolata culture systems of the sub-tidal zone and the pond mulched plastic film and sand in bottom were analyzed at molecular level by adopting the denaturing gradient gel electrophoresis (DGGE). The results indicated that the dominant bacterial communities in Babylonia areolata culture systems of the sub-tidal zone and the pond mulched plastic film and sand in bottom, which were built on the basis of the seawater in East-island of Zhanjiang, included Proteobac- teda Chloroflexi, Cyanobacteria and Actinobacteria. The dominant bacterial groups in the above pond culture system were Garnmaproteobacteria, Alphaproteobacteria, Deltaprotecbacteda, Epsilonproteobacteda, Anaerolineae, Cyanobacteria and Acti- nobacteda. The dominant bacterial communities in the subtidal zone culture system were Gammaprotecbacteda, Alphaproteobacteria, Deltaproteobacteria, Anaerolineae and Cyanobacteda, and there were less Epsilonproteobacteria and Actinobacteria in the culture system. The higher diversity was detected in the above two culture sys- tems. The results of unweighted pair group method with arithmetic average (UPG- MA) showed that the bacterial communities of the sediment samples S1 and S2 in the above two culture systems were a cluster, the similarity of bacterial communities was 54.5%. The bacterial communities of seawater samples S3 and S4 in the above culture systems were in clusters, and the similarity of the bacterial communi- ties was 84.0%. The results showed that the microorganism ecological level in the Babylonia areolata culture systems of the pond mulched plastic film and sand in bottom could be similar to the sub-tidal zone culture systems through changing the pond seawater and monitoring the microbial population.
基金supported by the Zhejiang Provincial Natural Science Foundation of China(LGN22C200027 and LZ23C200001).
文摘Studies showed that complexation of polyphenols with milk allergens reduced their immunogenic potential.However,the relationship between structures of polyphenols and their hypoallergenic effects on milk allergens in association with physiological and conformational changes of the complexes remain unclear.In this study,polyphenols from eight botanical sources were extracted to prepare non-covalent complexes withβ-lactoglobulin(β-LG),a major allergen in milk.The dominant phenolic compounds bound toβ-LG with a diminished allergenicity were identified to investigate their respective role on the structural and allergenic properties ofβ-LG.Extracts from Vaccinium fruits and black soybeans were found to have great inhibitory effects on the IgE-and IgG-binding abilities ofβ-LG.Among the fourteen structure-related phenolic compounds,flavonoids and tannins with larger MWs and multi-hydroxyl substituents,notably rutin,EGCG,and ellagitannins were more potent to elicit changes on the conformational structures ofβ-LG to decrease the allergenicity of complexedβ-LG.Correlation analysis further demonstrated that a destabilized secondary structure and protein depolymerization caused by polyphenol-binding were closely related to the allergenicity property of formed complexes.This study provides insights into the understanding of structure-allergenicity relationship ofβ-LG-polyphenol interactions and would benefit the development of polyphenol-fortified matrices with hypoallergenic potential.
基金the National Natural Science Foundation of China (Nos.30671207 and 40371063).
文摘Biolog, 16S rRNA gene denaturing gradient gel electrophoresis (DGGE), and phospholipid fatty acid (PLFA) analyses were used to assess soil microbial community characteristics in a chronosequence of tea garden systems (8-, 50-, and 90- year-old tea gardens), an adjacent wasteland, and a 90-year-old forest. Biolog analysis showed that the average well color development (AWCD) of all carbon sources and the functional diversity based on the Shannon index decreased (P 〈 0.05) in the following order: wasteland 〉 forest 〉 tea garden. For the DCCE analysis, the genetic diversity based on the Shannon index was significantly lower in the tea garden soils than in the wasteland. However, compared to the 90-year-old forest, the tea garden soils showed significantly higher genetic diversity. PLFA analysis showed that the ratio of Gram positive bacteria to Cram negative bacteria was significantly higher in the tea garden soils than in the wasteland, and the highest value was found in the 90-year-old forest. Both the fungal PLFA and the ratio of fungi to bacteria were significantly higher in the three tea garden soils than in the wasteland and forest, indicating that fungal PLFA was significantly affected by land-use change. Based on cluster analysis of the soil microbial community structure, all three analytical methods showed that land-use change had a greater effect on soil microbial community structure than tea garden age.
基金supported by the High-Tech Research Program (863) of China (No.2009ZX07106-002-004)the National Natural Science Foundation of China (No.30870221,20877093,50808172, 50909091)
文摘Effects of suction dredging on water quality and zooplankton community structure in a shallow of eutrophic lake, were evaluated. The results showed that a decreasing trend for levels of phosphorus, organic matter, total suspended solids, Chlorophyll a and Secchi transparency in the water column was found, while levels of water depth, electrical conductivity, total dissolved solids and NO3^--N concentration increased markedly post-dredging. The effects of dredging on dissolved oxygen, pH value and temperature were almost negligible. The zooplankton community structure responded rapidly to the environmental changes caused mainly by dredging. As a result, the abundance of rotifers decreased, while the density of zooplanktonic crustaceans increased markedly. The representative taxa were Brachionus angularis, B. budapestinensis, B. diversicornis, Synchaeta spp. and Neodiaptomus schmackeri. A distinct relationship between zooplankton taxa composition and their environment, unraveled by a redundancy analysis, indicating that the measured environment contributed to the variations in the zooplankton community structure to some extent. The first four synthetic environmental variables explained 51.7% of the taxonomic structure. Therefore, with the reduction of internal nutrient load and a shift in dominance by less eutrophic species, it inferred that dredging might be one of effective measures for environmental improvements of such lakes.
基金supported by the Natural Science Foundation of Jiangsu Province (No.BK2006710) the Hi-Tech Research and Development Program (863) of China (No:2003AA601100)
文摘Increasing levels of pollution within water bodies can cause eutrophication and an associated rapid growth in and reproduction of phytoplankton. Although most frequently occurring in bodies of water such as lakes and dams, in recent years an increasing number of river systems in China have suffered serious algal blooms. The community structure of phytoplankton may differ, however, dependent on the hydrodynamic conditions and nutrient levels within the water body. The field investigation results obtained from a stagnant river in Suzhou City and Taihu Lake, China, showed that in water with higher concentrations of nitrogen and phosphorus, Chlorophyta became the predominant species and in water with lower concentrations of nitrogen and phosphorus, Cyanobacteria became the predominant species. Growth experiments with competitive species, Microcystis aeruginosa Kutz and Scenedesmus quadricauda (Turp.), were conducted at three different nutrient levels. The biomass of algae in pure and mixed cultures was measured under conditions of different N/P ratios at oligotrophic, eutrophic and hypertrophic nutrient levels. The results indicated that the most suitable state for the growth and reproduction of M. aeruginosa and S. quadricauda were eutrophic conditions in both pure and mixed cultures. Under competition, however, the lower medium nutrient levels favoured M. aeruginosa, while the higher medium nutrient levels better suited S. quadricauda. Under similar hydrodynamic conditions, the community structure of phytoplankton in the water body was determined by the dominant species in competition for nutrients.
文摘On the basis of data collected in the summer of 2006 from 27 sampling stations in the Changjiang Estuary and its adjacent waters, the ecological characteristics of macrobenthos and the relationship between the macrobenthos and the environmental factors were studied using hierarchical cluster and non-metric multidimensional scaling (MDS). The biomass, abundance, Shannon -Wiener's and Margalef' s indices of the macrobenthos were presented. The results showed that a total of 253 macrobenthic species were found in the research region, and most of them belong to mollusks and polychaetes. The dominant species were Cossurella dimorpha, Eocylichna cylindrella, Episiphon kiaochowwanense, Nassarius semiplicatus, Ocstergrenia variabilis and Sternaspis scutata. The average abundance of the macrobenthos was (313. 15 ±233.4) ind./m^2, and the average biomass was ( 15. 2 ± 11.2 )g/m^2. The distribution patterns of the abundance and biomass of the macrobenthos were similar. The abundance and biomass in the area close to the estuary were lower than those from the area more distant to the estuary; the central part of the research region had higher abundance and biomass than other parts of the research region. In accordance with the results, four macrobenthic communities with distinct spatial differences were identified. The low abundance and biomass in the area close to the estuary should be caused by the high sedimentation rate. The statistical analysis indicated that the depth is the most important factor affecting the distribution of macrobenthos.
文摘Here, using the Scale-Symmetric Theory (SST) we explain the cosmological tension and the origin of the largest cosmic structures. We show that a change in value of strong coupling constant for cold baryonic matter leads to the disagreement in the galaxy clustering amplitude, quantified by the parameter S8. Within the same model we described the Hubble tension. We described also the mechanism that transforms the gravitational collapse into an explosion—it concerns the dynamics of virtual fields that lead to dark energy. Our calculations concern the Type Ia supernovae and the core-collapse supernovae. We calculated the quantized masses of the progenitors of supernovae, emitted total energy during explosion, and we calculated how much of the released energy was transferred to neutrinos. Value of the speed of sound in the strongly interacting matter measured at the LHC confirms that presented here model is correct. Our calculations show that the Universe is cyclic.