Objective To compare the quality and quantity of total RNA from different source-original neurons applied in LMPC technique. Methods （ 1 ） Aglient 2100 bioanalyzer and RT-PCR were used to check the concentration and...Objective To compare the quality and quantity of total RNA from different source-original neurons applied in LMPC technique. Methods （ 1 ） Aglient 2100 bioanalyzer and RT-PCR were used to check the concentration and fragmentation of total RNA from unfixed, temporal fixed and fixed 12 h hypothalamus sections; （2）Different neurons of PVN and SON were collected by LMPC, CRH, TRH, AVP, OT mRNA level were measured by RT-PCR; （3）Labeled neurons by injecting CTB into stomach and non-labeled neurons in DMV collected by LMPC were checked for house keeping genes by RT-PCR. Results （ 1 ） Unfixed section had higher concentration and better quality of total RNA compared with fixed sections applied in LMPC ; relative short amplicons such as GAPDH, NSE, MCH and MCAR were successfully obtained from fixed and unfixed and long amplicon of GR can only be obtained from unfixed material; （2） In magnocellular PVN and SON the expressions of AVP and OT were more special than those in the parvocellular PVN. Oppositely, the expressions of CRH, TRH in the parvocellular were more special than the other two ; （3） The expressions of house keeping genes had no significant difference between labeled and non-labeled DMV neurons. Conclusion The quality and quantity of total RNA from unfixed brain tissues were better than fixed tissues applied in LMPC and the CTB tracer which may differentiate neurons had no significant effect on physiology of the neurons applied in LMPC. The results showed that the LMPC technique is suitable for the qualitative and quantitative study on individual neurons at mRNA level.展开更多
We discuss two-stage iterative methods for the solution of linear systemAx = b, and give a new proof of the comparison theorems of two-stage iterative methodfor an Hermitian positive definite matrix. Meanwhile, we put...We discuss two-stage iterative methods for the solution of linear systemAx = b, and give a new proof of the comparison theorems of two-stage iterative methodfor an Hermitian positive definite matrix. Meanwhile, we put forward two new versionsof well known comparison theorem and apply them to some examples.展开更多
The study explores the intricate interplay between land use land cover(LULC),normalized difference vegetation index(NDVI),and land surface temperature(LST)within the Lower Son River Basin in India from 1991 to 2020.Th...The study explores the intricate interplay between land use land cover(LULC),normalized difference vegetation index(NDVI),and land surface temperature(LST)within the Lower Son River Basin in India from 1991 to 2020.The region’s ecological balance has been increasingly strained due to rapid urbanization and changing land use patterns.Through a combination of Landsat TM&OLI/TIRS satellite imageries and geospatial analysis techniques,this study unveils the intricate connection between land use and land cover changes,vegetation,and land surface temperature variations.The study area is classified into three altitudinal zones(Zone Ⅰ:39–300 m,Zone Ⅱ:301–600 m and Zone Ⅲ:601–1,247 m)to examine the changes in depth.The area has seen significant changes in LULC,vegetation and LST in all the three altitudinal zones.The findings hold significant implications for sustainable land management and environmental conservation strategies in the Lower Son River Basin.As per the result,103,438 ha of vegetation was converted into agriculture land and 82,572 ha of agricultural land was transformed into settlements from 1991 to 2020.This trend shows human pressure on the land resource in the study area.Minor increase in water body is seen which is attributed to commissioning of Bansagar dam.Zone Ⅰ has seen highest settlement growth while Zone Ⅲ experienced severe deforestation of around 15%.Zone Ⅱ and Ⅲ needs attention for holistic sustenance.Analysis of LST shows that it has increased by 0.82℃ from 1991 to 2020 which is a red flag.The study underscores the critical importance of balanced land use practices to preserve ecological integrity and mitigate the adverse effects of urbanization and climate change.展开更多
提出了一种面向自组织网络SON(self-organizing networks)的单播路由协议,支持应用服务质量QoS(quality of service)需求和路由器生物行为.基于OSPFv3/BGP4+,设计了自治域内和自治域间路由协议,包括报文格式与功能、链路状态描述结构、...提出了一种面向自组织网络SON(self-organizing networks)的单播路由协议,支持应用服务质量QoS(quality of service)需求和路由器生物行为.基于OSPFv3/BGP4+,设计了自治域内和自治域间路由协议,包括报文格式与功能、链路状态描述结构、邻居信息表、单播路由表和协议工作流程等.基于Quagga软件路由器实现了提出的协议,并且在网络实验平台基于Iperf工具对其进行了性能评测.结果表明,该协议是有效的,具有较好的性能.展开更多
Lawrence's novels are perhaps the most powerful exploration in the genre in English of family,class,sexuality and relationships in youth and early adulthood.Since the birth of this novel,varies comments on it were...Lawrence's novels are perhaps the most powerful exploration in the genre in English of family,class,sexuality and relationships in youth and early adulthood.Since the birth of this novel,varies comments on it were continuous.This analysis briefly introduces the personality of the main character-Paul Morel by means of specific description in the story.展开更多
文摘Objective To compare the quality and quantity of total RNA from different source-original neurons applied in LMPC technique. Methods （ 1 ） Aglient 2100 bioanalyzer and RT-PCR were used to check the concentration and fragmentation of total RNA from unfixed, temporal fixed and fixed 12 h hypothalamus sections; （2）Different neurons of PVN and SON were collected by LMPC, CRH, TRH, AVP, OT mRNA level were measured by RT-PCR; （3）Labeled neurons by injecting CTB into stomach and non-labeled neurons in DMV collected by LMPC were checked for house keeping genes by RT-PCR. Results （ 1 ） Unfixed section had higher concentration and better quality of total RNA compared with fixed sections applied in LMPC ; relative short amplicons such as GAPDH, NSE, MCH and MCAR were successfully obtained from fixed and unfixed and long amplicon of GR can only be obtained from unfixed material; （2） In magnocellular PVN and SON the expressions of AVP and OT were more special than those in the parvocellular PVN. Oppositely, the expressions of CRH, TRH in the parvocellular were more special than the other two ; （3） The expressions of house keeping genes had no significant difference between labeled and non-labeled DMV neurons. Conclusion The quality and quantity of total RNA from unfixed brain tissues were better than fixed tissues applied in LMPC and the CTB tracer which may differentiate neurons had no significant effect on physiology of the neurons applied in LMPC. The results showed that the LMPC technique is suitable for the qualitative and quantitative study on individual neurons at mRNA level.
基金This work is supported by NSF of Shanxi province,20011041.
文摘We discuss two-stage iterative methods for the solution of linear systemAx = b, and give a new proof of the comparison theorems of two-stage iterative methodfor an Hermitian positive definite matrix. Meanwhile, we put forward two new versionsof well known comparison theorem and apply them to some examples.
文摘The study explores the intricate interplay between land use land cover(LULC),normalized difference vegetation index(NDVI),and land surface temperature(LST)within the Lower Son River Basin in India from 1991 to 2020.The region’s ecological balance has been increasingly strained due to rapid urbanization and changing land use patterns.Through a combination of Landsat TM&OLI/TIRS satellite imageries and geospatial analysis techniques,this study unveils the intricate connection between land use and land cover changes,vegetation,and land surface temperature variations.The study area is classified into three altitudinal zones(Zone Ⅰ:39–300 m,Zone Ⅱ:301–600 m and Zone Ⅲ:601–1,247 m)to examine the changes in depth.The area has seen significant changes in LULC,vegetation and LST in all the three altitudinal zones.The findings hold significant implications for sustainable land management and environmental conservation strategies in the Lower Son River Basin.As per the result,103,438 ha of vegetation was converted into agriculture land and 82,572 ha of agricultural land was transformed into settlements from 1991 to 2020.This trend shows human pressure on the land resource in the study area.Minor increase in water body is seen which is attributed to commissioning of Bansagar dam.Zone Ⅰ has seen highest settlement growth while Zone Ⅲ experienced severe deforestation of around 15%.Zone Ⅱ and Ⅲ needs attention for holistic sustenance.Analysis of LST shows that it has increased by 0.82℃ from 1991 to 2020 which is a red flag.The study underscores the critical importance of balanced land use practices to preserve ecological integrity and mitigate the adverse effects of urbanization and climate change.
文摘提出了一种面向自组织网络SON(self-organizing networks)的单播路由协议,支持应用服务质量QoS(quality of service)需求和路由器生物行为.基于OSPFv3/BGP4+,设计了自治域内和自治域间路由协议,包括报文格式与功能、链路状态描述结构、邻居信息表、单播路由表和协议工作流程等.基于Quagga软件路由器实现了提出的协议,并且在网络实验平台基于Iperf工具对其进行了性能评测.结果表明,该协议是有效的,具有较好的性能.
文摘Lawrence's novels are perhaps the most powerful exploration in the genre in English of family,class,sexuality and relationships in youth and early adulthood.Since the birth of this novel,varies comments on it were continuous.This analysis briefly introduces the personality of the main character-Paul Morel by means of specific description in the story.
