Summary： The effect of the complement Clq expression on total hepatic ischemia-reperfusion （I/R） injury in rats was investigated. Sixty healthy male Sprague Dawley （SD） rats weighing 180-200 g were randomly divid...Summary： The effect of the complement Clq expression on total hepatic ischemia-reperfusion （I/R） injury in rats was investigated. Sixty healthy male Sprague Dawley （SD） rats weighing 180-200 g were randomly divided into 5 groups： sham-operation group （S group, n=12）; group of I/R for 1 h （FR 1 h group, n=12）; group of I/R for 3 h （I/R 3 h group, n=12）; group of I/R for 6 h （I/R 6 h group, n=12）; group of UR for 24 h （I/R 24 h group, n=12）. The hepatic I/R model of rats was established, and liver tissues were obtained 1 h, 3 h, 6 h and 24 h after hepatic I/R, respectively. Furthermore, the tissues were stained using hematoxylin-eosin, and the liver injuries of rats were observed using a microscope. The malondialdehyde （MDA） level and superoxide dismutase （SOD） activity in liver tissue were determined Real-time polymerase chain reaction （PCR） and Western blotting were used to detect the expression levels of Clq mRNA and protein, respectively. As compared with the S group, the histopathological changes in I/R 1 h-24 h groups were gradually aggravated with the extension of FR time. As compared with the S group, SOD activity and MDA content in the I/R groups were reduced and increased respec- tively with the extension of UR time （P〈0.01）. Furthermore, the Clq expression at mRNA and protein levels in the I/R groups （especially in the I/R 3 h group） was significantly higher than that in the S group （P〈0.05）. It is suggested that Clq expression may play a principal role in hepatic I/R injury, particularly at the early stage ofperfusion.展开更多
基金supported by the National Natural SciencFoundation of China(No.2013CFB247)
文摘Summary： The effect of the complement Clq expression on total hepatic ischemia-reperfusion （I/R） injury in rats was investigated. Sixty healthy male Sprague Dawley （SD） rats weighing 180-200 g were randomly divided into 5 groups： sham-operation group （S group, n=12）; group of I/R for 1 h （FR 1 h group, n=12）; group of I/R for 3 h （I/R 3 h group, n=12）; group of I/R for 6 h （I/R 6 h group, n=12）; group of UR for 24 h （I/R 24 h group, n=12）. The hepatic I/R model of rats was established, and liver tissues were obtained 1 h, 3 h, 6 h and 24 h after hepatic I/R, respectively. Furthermore, the tissues were stained using hematoxylin-eosin, and the liver injuries of rats were observed using a microscope. The malondialdehyde （MDA） level and superoxide dismutase （SOD） activity in liver tissue were determined Real-time polymerase chain reaction （PCR） and Western blotting were used to detect the expression levels of Clq mRNA and protein, respectively. As compared with the S group, the histopathological changes in I/R 1 h-24 h groups were gradually aggravated with the extension of FR time. As compared with the S group, SOD activity and MDA content in the I/R groups were reduced and increased respec- tively with the extension of UR time （P〈0.01）. Furthermore, the Clq expression at mRNA and protein levels in the I/R groups （especially in the I/R 3 h group） was significantly higher than that in the S group （P〈0.05）. It is suggested that Clq expression may play a principal role in hepatic I/R injury, particularly at the early stage ofperfusion.
