The topical administration of the compound Chinese herbal medicine transdermal preparation has been widely used in treating the mastitis of cows. In order to understand the metabolic process, four cows suffering from ...The topical administration of the compound Chinese herbal medicine transdermal preparation has been widely used in treating the mastitis of cows. In order to understand the metabolic process, four cows suffering from clinical mastitis were selected for the pharmacokinetic study. The transdermal preparation was applied to the diseased part of breast. Then the plasma and milk samples were collected respectively at 0, 0.5, 1, 2, 4, 6, 8, 10, 12, 24, and 36 h, The concentrations of baicalin and phillyrin in plasma and milk were determined by HPLC and the data of time-concentrations were analyzed by the pharmacokinetic software. The results showed that two ingredients in the plasma were not detectable, but they in the milk had linear relationship with the time. The peak concentration of baicalin [（2.84 ±0.15）μg mL^-1] appeared at （4.93 ± 0.35） h, AUC0.1OQ was （27.32 ± 7.91） mg L^-1 h^-1, and the mean retention time was （28.31 ±0.49） h. The peak concentration of phillyrin [（0.49 ± 0.41） μg mL^-1] achieved at （3.68 ± 3.14） h, AUC0-LOQ was （4.10± 0.31） mg L^-1 h^-1, and the mean retention time was （14.52 ± 0.02） h. These suggested that two ingredients had slow absorbing speed and high absorbing degree. They could not be eliminated in a short time, thus exerted the topical curative effect.展开更多
[Objective] To develop a new antiviral agent with high and long efficacy, wide spectrum, low toxicity and low cost from traditional Chinese herbal medicine and their compound. [ Method] The ＂Quxie＂ （ i. e. eliminat...[Objective] To develop a new antiviral agent with high and long efficacy, wide spectrum, low toxicity and low cost from traditional Chinese herbal medicine and their compound. [ Method] The ＂Quxie＂ （ i. e. eliminating pathogenic factors） drugs including Hedyotis diffusa and Loni- cera japonica and ＂Fuzheng＂ （ i. e. strengthening body resistance） drugs including Radix astragali and Glycyrrhiza uralensis were taken to form an antiviral herbal compound preparation. Taking the astragalus polysaccharide monomer as control, the experiment was constructed by using the agent to treat some naturally occurring viral diseases, including Newcastle disease （ND）, infectious bursal disease （IBD）, duck viral hepatitis （DVH）, avian influenza （Al） H9 subtype and avian swollen head syndrome. [Ressult] The effective rates of the agent for treatment of the above- mentioned diseases were 87%, 87%, 69%, 100% and 100%, respectively, which were higher than that of astragalus polysaccharide monomer. The cure rates for these diseases were 33%, 37%, 13%, 69% and 75%, respectively, which were significantly higher than that of astragalus polysacchadde monomer （ P 〈 0.01 ）. The rates of poor therapeutic efficacy were 15%, 21%, 24%, 6% and 7%, respectively, which were significantly lower than that of astragalus polysaccharide monomer （P 〈 0.01 ）. [ Condusion] The Chinese herbal compound preparation shows good therapeutic effects on some avian viral diseases, which can decrease mortality rate and improve production performance of poultry more greatly than astragalus polysaccharide monomer.展开更多
As an important representative of the modernization of the Chinese medicine industry,traditional Chinese medicine(TCM)compound preparations are an indispensable part of the development of TCM.The complex ingredients a...As an important representative of the modernization of the Chinese medicine industry,traditional Chinese medicine(TCM)compound preparations are an indispensable part of the development of TCM.The complex ingredients and unclear action mechanism of TCM compound preparations restrict the development of TCM.In line with this problem,the modern TCM research has made extensive remarkable achievements.From the single indicator in the past to multiple indicator,the research on the combination of TCM fingerprints and pharmacodynamics also has made great progress.The introduction of quality evaluation methods such as the"quantitative analysis of multi-components by a single marker","dose-effect"indicator,"Bioactive Equivalent Combinatorial Components"(BECCs)and TCM quality markers have brought new ideas for quality control of TCM compound preparations.展开更多
Stroke is one of the leading causes of death and disability in adults worldwide,resulting in huge social and financial burdens.Extracts from herbs,especially those used in Chinese medicine,have emerged as new pharmace...Stroke is one of the leading causes of death and disability in adults worldwide,resulting in huge social and financial burdens.Extracts from herbs,especially those used in Chinese medicine,have emerged as new pharmaceuticals for stroke treatment.Here we review the evidence from preclinical studies investigating neuroprotective properties of Chinese medicinal compounds through their application in acute and subacute phases of ischemic stroke,and highlight potential mechanisms underlying their therapeutic effects.It is noteworthy that many herbal compounds have been shown to target multiple mechanisms and in combinations may exert synergistic effects on signaling pathways,thereby attenuating multiple aspects of ischemic pathology.We conclude the paper with a general discussion of the prospects for novel natural compound-based regimens against stroke.展开更多
[Objectives]To explore the effects of nano compound Chinese herbal medicine feed additive on growth performance,meat quality and disease resistance of chickens.[Methods]Chickens were fed with nano compound Chinese her...[Objectives]To explore the effects of nano compound Chinese herbal medicine feed additive on growth performance,meat quality and disease resistance of chickens.[Methods]Chickens were fed with nano compound Chinese herbal medicine feed additive developed by Hunan Polytechnic of Environment and Biology,including 120 chickens in the treatment group and 120 chickens in the control group(CK).The growth performance indices(body weight gain,feed to gain ratio and slaughter index),meat quality indices(pH value,color,drip loss,shear force)and disease resistance indices(morbidity and mortality)of the chickens in the treatment and CK groups were recorded and determined,respectively.[Results]The inclusion of 2%nano compound Chinese herbal medicine feed additive in the diet significantly increased the growth rate,reduced the feed-to-gain ratio and improved the meat quality of the chickens.Supplementing Chinese herbal medicine could increase the pH value and reduce the drip loss and shear force of chicken meat.At the same time,the body's immune function,antioxidant level and resistance against diseases of the chickens fed with nano compound Chinese herbal medicine feed additive were improved.[Conclusions]The inclusion of nano compound Chinese herbal medicine feed additive in the diet can improve the growth performance,meat quality and disease resistance of chickens.展开更多
With the interdisciplinary development of multiple disciplines,mathematical models play a role as a bridge in the research process of Chinese herbal compounds,which can make full use of the interrelationships of knowl...With the interdisciplinary development of multiple disciplines,mathematical models play a role as a bridge in the research process of Chinese herbal compounds,which can make full use of the interrelationships of knowledge,making the research and use of Chinese herbal compounds from complex and cumbersome to simple.This paper reviewed the research and application of mathematical models in the production and preparation,pharmacodynamics,pharmacokinetics and other aspects of Chinese herbal compound prescriptions,providing a theoretical basis for the development of the modern Chinese medicine industry.展开更多
[Objectives]To study the effects of fermented nano Chinese herbal medicines replacing antibiotics on the production performance and carcass quality of growing-finishing pigs.[Methods]200 healthy and disease-free Duroc...[Objectives]To study the effects of fermented nano Chinese herbal medicines replacing antibiotics on the production performance and carcass quality of growing-finishing pigs.[Methods]200 healthy and disease-free Duroc-Landrace-Yorkshire(DLY)growing-finishing pigs with similar birth time(45±2 d)and body weight of about 31.5 kg were randomly divided into group A(fed with non-antibiotic basal diet),group B(fed with antibiotic basal diet),group C(fed with non-antibiotic basal diet+0.1%fermented nano Chinese herbal medicine preparation),group D(fed with non-antibiotic basal diet+0.3%fermented nano Chinese herbal medicine preparation)and group E(fed with non-antibiotic basal diet+0.5%fermented nano Chinese herbal medicine preparation).The experiment lasted for 90 d.At the end of the experiment,the production performance and carcass indicators were measured.[Results]Compared with group A,the final weight,average daily gain(ADG)and average daily feed intake(ADFI)of groups B,C,D and E were extremely significantly increased(P<0.01),while the feed-to-gain ratio(F/G)was extremely significantly decreased(P<0.01).Compared with group B,the ADG of group E was significantly increased(P<0.05),while the F/G was significantly decreased(P<0.05).Compared with group A,the slaughter rate and carcass length of pigs in groups B,C,D and E were extremely significantly increased(P<0.01);compared with group B,the slaughter rate of groups D and E was extremely significantly increased(P<0.01).[Conclusions]The fermented nano Chinese herbal medicine preparation has better effects in promoting growth than that of antibiotic additives,and it can significantly improve the carcass quality of growing-finishing pigs.展开更多
Serum pharmacochemistry of traditional Chinese medicine has become a more accurate and rapidly developing reasonable way to analyze the effective material basis of traditional Chinese medicine in recent years. Through...Serum pharmacochemistry of traditional Chinese medicine has become a more accurate and rapidly developing reasonable way to analyze the effective material basis of traditional Chinese medicine in recent years. Through this method, we can select very complex Chinese medicine components and clarify the effective substances consistent with the main functions, and then separate and purify effective ingredients in traditional Chinese medicine, so as to explain the material basis of the drug effect and prove the rationality and principle of action of Chinese medicine compounds. It can show the multi-component and multi-target characteristic of traditional Chinese medicine, and after analyzing the migrating components of serum, we can explore the direct effective substances that act on the patient’s body, so as to accelerate and accurately complete the in-depth study of the effective substances of Chinese medicine.展开更多
研究探讨了小球藻替代鱼粉下,不同剂量的复方中草药(Chinese Herbal Medicine Compound,CHMC)对大口黑鲈(Micropterus salmoides)肌肉品质的影响。实验在大口黑鲈基础饲料配方中,以小球藻粉完全替代其中的鱼粉,并随后以2%、4%和6%水平...研究探讨了小球藻替代鱼粉下,不同剂量的复方中草药(Chinese Herbal Medicine Compound,CHMC)对大口黑鲈(Micropterus salmoides)肌肉品质的影响。实验在大口黑鲈基础饲料配方中,以小球藻粉完全替代其中的鱼粉,并随后以2%、4%和6%水平分别添加复方中草药汤剂于小球藻粉替代组中,制作成等氮(CP:52%)等脂(CL:10%)的五组实验饲料,分别记为FM、CM、CMH2、CMH4和CMH6组。将健康的大口黑鲈225尾[均重(100.20±0.29)g]随机分为5组,每组3个重复,每个重复15尾,分别投喂以上5种实验饲料,饲养时间80d。结果表明:(1)CMH2、CMH4和CMH6组大口黑鲈侧线部皮肤亮度值(L*)均显著高于FM与CM组(P<0.05),且CMH2、CMH4和CMH6组大口黑鲈肌肉黄色度值(b*)均显著高于CM组(P<0.05)。(2)CMH2、CMH4和CMH6组大口黑鲈肌肉粗脂肪含量均显著低于FM与CM组(P<0.05),肌肉粗蛋白、水分和粗灰分含量在各组间均无显著差异(P>0.05)。(3)CMH6组大口黑鲈肌肉风味氨基酸含量、必需氨基酸含量和总氨基酸含量均显著高于FM组和CM组(P<0.05),CMH4组大口黑鲈肌肉C16:1n-7脂肪酸水平显著高于CM组(P>0.05)。(4)鱼体肌肉内聚性、弹性及滴水损失在各组间均无显著差异(P>0.05),但随复方中草药添加量的上升,鱼体肌肉硬度、黏附性、胶黏性、咀嚼性、剪切力及pH均显著升高(P<0.05)。(5)CMH4组和CMH6组鱼体肌纤维直径均显著低于CM组(P<0.05),而该中草药组鱼体肌纤维密度均显著高于CM组(P<0.05)。(6)大口黑鲈肌肉MyoD与MyoG等生肌调节因子基因相对表达量在各组间均无显著差异(P>0.05),但MSTN肌肉生长抑制素基因的相对表达量在CMH6组显著降低(P<0.05)。综上表明,在饲料中添加4%—6%复方中草药改善了小球藻替代鱼粉下大口黑鲈体色与肉色,提高了鱼体肌肉营养成分含量及其肌肉质构,该结果可能与复方中草药抑制了MSTN基因表达从而促进了肌纤维的生长有关。展开更多
基金supported by the Major Project of Re-vitalizing the Old Industrial Bases of Science and Edu-cation Commission of Heilongjiang Province, China(1151gzd04)the 11th Five-Year Plan Major Re-search Projects of Science and Technology Commis-sion of Heilongjiang Province (GA06B202-3)
文摘The topical administration of the compound Chinese herbal medicine transdermal preparation has been widely used in treating the mastitis of cows. In order to understand the metabolic process, four cows suffering from clinical mastitis were selected for the pharmacokinetic study. The transdermal preparation was applied to the diseased part of breast. Then the plasma and milk samples were collected respectively at 0, 0.5, 1, 2, 4, 6, 8, 10, 12, 24, and 36 h, The concentrations of baicalin and phillyrin in plasma and milk were determined by HPLC and the data of time-concentrations were analyzed by the pharmacokinetic software. The results showed that two ingredients in the plasma were not detectable, but they in the milk had linear relationship with the time. The peak concentration of baicalin [（2.84 ±0.15）μg mL^-1] appeared at （4.93 ± 0.35） h, AUC0.1OQ was （27.32 ± 7.91） mg L^-1 h^-1, and the mean retention time was （28.31 ±0.49） h. The peak concentration of phillyrin [（0.49 ± 0.41） μg mL^-1] achieved at （3.68 ± 3.14） h, AUC0-LOQ was （4.10± 0.31） mg L^-1 h^-1, and the mean retention time was （14.52 ± 0.02） h. These suggested that two ingredients had slow absorbing speed and high absorbing degree. They could not be eliminated in a short time, thus exerted the topical curative effect.
文摘[Objective] To develop a new antiviral agent with high and long efficacy, wide spectrum, low toxicity and low cost from traditional Chinese herbal medicine and their compound. [ Method] The ＂Quxie＂ （ i. e. eliminating pathogenic factors） drugs including Hedyotis diffusa and Loni- cera japonica and ＂Fuzheng＂ （ i. e. strengthening body resistance） drugs including Radix astragali and Glycyrrhiza uralensis were taken to form an antiviral herbal compound preparation. Taking the astragalus polysaccharide monomer as control, the experiment was constructed by using the agent to treat some naturally occurring viral diseases, including Newcastle disease （ND）, infectious bursal disease （IBD）, duck viral hepatitis （DVH）, avian influenza （Al） H9 subtype and avian swollen head syndrome. [Ressult] The effective rates of the agent for treatment of the above- mentioned diseases were 87%, 87%, 69%, 100% and 100%, respectively, which were higher than that of astragalus polysaccharide monomer. The cure rates for these diseases were 33%, 37%, 13%, 69% and 75%, respectively, which were significantly higher than that of astragalus polysacchadde monomer （ P 〈 0.01 ）. The rates of poor therapeutic efficacy were 15%, 21%, 24%, 6% and 7%, respectively, which were significantly lower than that of astragalus polysaccharide monomer （P 〈 0.01 ）. [ Condusion] The Chinese herbal compound preparation shows good therapeutic effects on some avian viral diseases, which can decrease mortality rate and improve production performance of poultry more greatly than astragalus polysaccharide monomer.
基金Supported by Project of National Natural Science Foundation of China(81560691)Special Fund Project for Innovation Driven Development of Guangxi(Gui Ke AA17202046)+8 种基金Program of Scientific Research Third-level Laboratory"Chinese(Zhuang)Medicine Chemical and Quality Analysis Laboratory"of State Administration of Traditional Chinese Medicine(Guo Zhong Yi Yao Fa 2009[21])Program of Key Laboratory for Purification and Quality Analysis of TCM Extraction in Guangxi Universities(Gui Jiao Ke Yan[2014]No.6)Program of Collaborative Innovation Center of Zhuang and Yao Medicine(Gui Jiao Ke Yan:2013 No.20)Program of Key Laboratory of Zhuang and Yao Medicine[Gui Ke Ji Zi:2014 No.32]Bagui Scholar Program of Key Discipline of Guangxi"Study on Innovation Theory and Efficacy of Traditional Chinese Medicine"[Gui Ke Zheng Zi:2013 No.25]High-level Talent Team Cultivation Program of"Qihuang Project"of Guangxi University of Chinese Medicine(2018002)Open Program for Construction of TCM Pharmacy Doctor Station in Guangxi University of Chinese Medicine(201410-06)High Level Innovation Team and Excellent Scholar Program of Guangxi Universities(Gui Jiao Shi Fan 2019 No.52)Guangxi One Thousand Young and Middle-aged College and University Backbone Teachers Cultivation Program(Gui Jiao Ren2019 No.5).
文摘As an important representative of the modernization of the Chinese medicine industry,traditional Chinese medicine(TCM)compound preparations are an indispensable part of the development of TCM.The complex ingredients and unclear action mechanism of TCM compound preparations restrict the development of TCM.In line with this problem,the modern TCM research has made extensive remarkable achievements.From the single indicator in the past to multiple indicator,the research on the combination of TCM fingerprints and pharmacodynamics also has made great progress.The introduction of quality evaluation methods such as the"quantitative analysis of multi-components by a single marker","dose-effect"indicator,"Bioactive Equivalent Combinatorial Components"(BECCs)and TCM quality markers have brought new ideas for quality control of TCM compound preparations.
基金supported by AHA Award 14SDG20480186(to LC)Kentucky Spinal Cord&Head Injury Research Trust Grant 14-12A(to KES)Startup Funds from Shaanxi University of Chinese Medicine to Young Investigators(1410170078)(to BZ)
文摘Stroke is one of the leading causes of death and disability in adults worldwide,resulting in huge social and financial burdens.Extracts from herbs,especially those used in Chinese medicine,have emerged as new pharmaceuticals for stroke treatment.Here we review the evidence from preclinical studies investigating neuroprotective properties of Chinese medicinal compounds through their application in acute and subacute phases of ischemic stroke,and highlight potential mechanisms underlying their therapeutic effects.It is noteworthy that many herbal compounds have been shown to target multiple mechanisms and in combinations may exert synergistic effects on signaling pathways,thereby attenuating multiple aspects of ischemic pathology.We conclude the paper with a general discussion of the prospects for novel natural compound-based regimens against stroke.
基金Project of Hunan Province for Science of Education during 13th Five-Year Plan Period[XJK18BZY066]Hengyang Social Science Foundation Project[2017B(1)010].
文摘[Objectives]To explore the effects of nano compound Chinese herbal medicine feed additive on growth performance,meat quality and disease resistance of chickens.[Methods]Chickens were fed with nano compound Chinese herbal medicine feed additive developed by Hunan Polytechnic of Environment and Biology,including 120 chickens in the treatment group and 120 chickens in the control group(CK).The growth performance indices(body weight gain,feed to gain ratio and slaughter index),meat quality indices(pH value,color,drip loss,shear force)and disease resistance indices(morbidity and mortality)of the chickens in the treatment and CK groups were recorded and determined,respectively.[Results]The inclusion of 2%nano compound Chinese herbal medicine feed additive in the diet significantly increased the growth rate,reduced the feed-to-gain ratio and improved the meat quality of the chickens.Supplementing Chinese herbal medicine could increase the pH value and reduce the drip loss and shear force of chicken meat.At the same time,the body's immune function,antioxidant level and resistance against diseases of the chickens fed with nano compound Chinese herbal medicine feed additive were improved.[Conclusions]The inclusion of nano compound Chinese herbal medicine feed additive in the diet can improve the growth performance,meat quality and disease resistance of chickens.
文摘With the interdisciplinary development of multiple disciplines,mathematical models play a role as a bridge in the research process of Chinese herbal compounds,which can make full use of the interrelationships of knowledge,making the research and use of Chinese herbal compounds from complex and cumbersome to simple.This paper reviewed the research and application of mathematical models in the production and preparation,pharmacodynamics,pharmacokinetics and other aspects of Chinese herbal compound prescriptions,providing a theoretical basis for the development of the modern Chinese medicine industry.
基金Supported by Natural Science Foundation of Hunan Province(2018JJ4032).
文摘[Objectives]To study the effects of fermented nano Chinese herbal medicines replacing antibiotics on the production performance and carcass quality of growing-finishing pigs.[Methods]200 healthy and disease-free Duroc-Landrace-Yorkshire(DLY)growing-finishing pigs with similar birth time(45±2 d)and body weight of about 31.5 kg were randomly divided into group A(fed with non-antibiotic basal diet),group B(fed with antibiotic basal diet),group C(fed with non-antibiotic basal diet+0.1%fermented nano Chinese herbal medicine preparation),group D(fed with non-antibiotic basal diet+0.3%fermented nano Chinese herbal medicine preparation)and group E(fed with non-antibiotic basal diet+0.5%fermented nano Chinese herbal medicine preparation).The experiment lasted for 90 d.At the end of the experiment,the production performance and carcass indicators were measured.[Results]Compared with group A,the final weight,average daily gain(ADG)and average daily feed intake(ADFI)of groups B,C,D and E were extremely significantly increased(P<0.01),while the feed-to-gain ratio(F/G)was extremely significantly decreased(P<0.01).Compared with group B,the ADG of group E was significantly increased(P<0.05),while the F/G was significantly decreased(P<0.05).Compared with group A,the slaughter rate and carcass length of pigs in groups B,C,D and E were extremely significantly increased(P<0.01);compared with group B,the slaughter rate of groups D and E was extremely significantly increased(P<0.01).[Conclusions]The fermented nano Chinese herbal medicine preparation has better effects in promoting growth than that of antibiotic additives,and it can significantly improve the carcass quality of growing-finishing pigs.
基金Supported by University Continuing Education Teaching Reform Project(2019jxjj67)Provincial quality engineering project of"Traditional Chinese Medicine Technique and Skilled Master Studio"of Anhui Provincial Department of Education in 2020(2020dsgzs23)+3 种基金Key Natural Science Research Project of Higher Learning Institutions in Anhui Province in 2018(KJ2018A0884)Key Natural Science Research Projects of Colleges and Universities in Anhui Province in 2020(KJ2020A1014)Key Project of 2020 Applied Technology Research and Collaborative Innovation Center of Bozhou Vocational and Technical College(BZZX202001)Key Research Project of Bozhou Vocational and Technical College in 2020(BYK2002)。
文摘Serum pharmacochemistry of traditional Chinese medicine has become a more accurate and rapidly developing reasonable way to analyze the effective material basis of traditional Chinese medicine in recent years. Through this method, we can select very complex Chinese medicine components and clarify the effective substances consistent with the main functions, and then separate and purify effective ingredients in traditional Chinese medicine, so as to explain the material basis of the drug effect and prove the rationality and principle of action of Chinese medicine compounds. It can show the multi-component and multi-target characteristic of traditional Chinese medicine, and after analyzing the migrating components of serum, we can explore the direct effective substances that act on the patient’s body, so as to accelerate and accurately complete the in-depth study of the effective substances of Chinese medicine.
文摘研究探讨了小球藻替代鱼粉下,不同剂量的复方中草药(Chinese Herbal Medicine Compound,CHMC)对大口黑鲈(Micropterus salmoides)肌肉品质的影响。实验在大口黑鲈基础饲料配方中,以小球藻粉完全替代其中的鱼粉,并随后以2%、4%和6%水平分别添加复方中草药汤剂于小球藻粉替代组中,制作成等氮(CP:52%)等脂(CL:10%)的五组实验饲料,分别记为FM、CM、CMH2、CMH4和CMH6组。将健康的大口黑鲈225尾[均重(100.20±0.29)g]随机分为5组,每组3个重复,每个重复15尾,分别投喂以上5种实验饲料,饲养时间80d。结果表明:(1)CMH2、CMH4和CMH6组大口黑鲈侧线部皮肤亮度值(L*)均显著高于FM与CM组(P<0.05),且CMH2、CMH4和CMH6组大口黑鲈肌肉黄色度值(b*)均显著高于CM组(P<0.05)。(2)CMH2、CMH4和CMH6组大口黑鲈肌肉粗脂肪含量均显著低于FM与CM组(P<0.05),肌肉粗蛋白、水分和粗灰分含量在各组间均无显著差异(P>0.05)。(3)CMH6组大口黑鲈肌肉风味氨基酸含量、必需氨基酸含量和总氨基酸含量均显著高于FM组和CM组(P<0.05),CMH4组大口黑鲈肌肉C16:1n-7脂肪酸水平显著高于CM组(P>0.05)。(4)鱼体肌肉内聚性、弹性及滴水损失在各组间均无显著差异(P>0.05),但随复方中草药添加量的上升,鱼体肌肉硬度、黏附性、胶黏性、咀嚼性、剪切力及pH均显著升高(P<0.05)。(5)CMH4组和CMH6组鱼体肌纤维直径均显著低于CM组(P<0.05),而该中草药组鱼体肌纤维密度均显著高于CM组(P<0.05)。(6)大口黑鲈肌肉MyoD与MyoG等生肌调节因子基因相对表达量在各组间均无显著差异(P>0.05),但MSTN肌肉生长抑制素基因的相对表达量在CMH6组显著降低(P<0.05)。综上表明,在饲料中添加4%—6%复方中草药改善了小球藻替代鱼粉下大口黑鲈体色与肉色,提高了鱼体肌肉营养成分含量及其肌肉质构,该结果可能与复方中草药抑制了MSTN基因表达从而促进了肌纤维的生长有关。
