Ulcerative colitis(UC)is a chronic refractory non-specific intestinal inflammatory disease that is difficult to be cured.The discovery of new ulcerative colitis-related metabolite biomarkers may help further understan...Ulcerative colitis(UC)is a chronic refractory non-specific intestinal inflammatory disease that is difficult to be cured.The discovery of new ulcerative colitis-related metabolite biomarkers may help further understand UC and facilitate early diagnosis.It may also provide a basis for explaining the mechanism of drug action in the treatment of UC.Compound Sophorae Decoction(CSD)is an empirical formula used in the clinical treatment of UC.Although it is known to be efficacious,its mechanism of action in the treatment of UC is unclear.The purpose of this study was to investigate the changes in endogenous substances in UC rats and the effects of CSD on metabolic pathways using the metabonomics approach.Metabolomics studies in rats with UC and normal rats were performed using LC-MS/MS.Rats with UC induced using TNBS enema were used as the study models.Metabolic profiling and pathway analysis of biomarkers was performed using statistical and pathway enrichment analyses.36 screened potential biomarkers were found to be significantly different between the UC and the normal groups;it was also found that CSD could modulate the levels of these potential biomarkers.CSD was found to be efficacious in UC by regulating multiple metabolic pathways.展开更多
OBJECTIVE: To investigate how compound Sophorae decoction(CSD) works on rats' models of ulcerative colitis(UC) induced by 2,4,6-trinitrobenzenesulfonic acid solution(TNBS) by metabolomics studies of colon, liver, ...OBJECTIVE: To investigate how compound Sophorae decoction(CSD) works on rats' models of ulcerative colitis(UC) induced by 2,4,6-trinitrobenzenesulfonic acid solution(TNBS) by metabolomics studies of colon, liver, and kidney tissue extracts.METHODS: Rats with UC induced by TNBS enema were used as models in this study. Metabolic profiles of the three tissues were analyzed and pathway analysis of biomarkers was performed after CSD administration and further integration of metabolic networks.RESULTS: Thirteen biomarkers were screened from colon, liver, and kidney tissue extracts, and the levels of these substances were up-or down-regulated in the model group, but their levels were reversed after CSD administration. These biomarkers were mainly related to Phenylalanine, tyrosine and tryptophan biosynthesis, Phenylalanine metabolism, Glutathione metabolism, Arachidonic acid metabolism, Nicotinate and nicotinamide metabolism,Alanine, aspartate and glutamate metabolism.CONCLUSION: CSD could significantly ameliorate the symptoms of UC by regulating multiple metabolic pathways.展开更多
The effects of compound Sophorae Flavescen-tis Jiechangrong capsule(CSFJC)on the expression of nuclear factor-κB p65(NF-κB p65)and signal transducer and activator of transcription 6(STAT6)in the intestinal mucosa of...The effects of compound Sophorae Flavescen-tis Jiechangrong capsule(CSFJC)on the expression of nuclear factor-κB p65(NF-κB p65)and signal transducer and activator of transcription 6(STAT6)in the intestinal mucosa of patients with ulcerative colitis and the possible mechanism were investigated.Eighteen patients with ulcerative colitis were randomly divided into a traditional Chinese medicine(TCM)group(n=11)treated by CSFJC and a western medicine(WM)group(n=7)treated by Sulfasalazine tablets.The treatment duration lasted eight weeks.Before and after the treatment,the symptoms and the physical signs were observed,and the routine stool test,the colonoscopy,and pathological examination were performed in the two groups.The expression levels of NF-κB p65 and STAT6 were detected by using immuno-histochemistry.The results showed that the total effective rate of the curative effectiveness in TCM and WM groups was 100%and 71.4%,respectively,and the total effective rate of colonic mucosa lesion in TCM and WM groups was 90.9%and 71.4%,respectively,with the differences being significant(all P<0.05).The total effective rate of syndromes of damp-heat blocking according to the TCM in TCM and WM groups was 90.9%and 71.4%,respectively.After the treatment,the expression of NF-κB p65 and STAT6 in the two groups was decreased,and the decrease of NF-κB p65 and STAT6 expression in TCM group was more significant than in WM group(P<0.05).It was concluded that CSFJC can inhibit the activation and expression of NF-κB p65 and STAT6 in the intestinal mucosa of patients with ulcerative colitis,which is a possible mechanism for CSFJC treating ulcerative colitis.展开更多
目的探讨复方苦参联合肝动脉化疗栓塞术(chemoembolization of the hepatic artery,TACE)治疗原发性肝癌对患者术后不良反应及肝功能损伤的影响。方法随机选取2021年1月—2023年1月徐州市肿瘤医院接诊的原发性肝癌患者80例,利用随机数...目的探讨复方苦参联合肝动脉化疗栓塞术(chemoembolization of the hepatic artery,TACE)治疗原发性肝癌对患者术后不良反应及肝功能损伤的影响。方法随机选取2021年1月—2023年1月徐州市肿瘤医院接诊的原发性肝癌患者80例,利用随机数表法分为两组。对照组40例患者接受TACE进行治疗,观察组患者40例患者则采取TACE+复方苦参治疗。分析两组患者术后不良反应发生率、不良反应严重程度以及术后肝功能损伤程度。结果观察组患者术后不良反应发生率为10.00%,较对照组的30.00%更低,差异有统计学意义(χ^(2)=5.000,P=0.025);治疗前,两组患者疼痛评分和肝功能指标比较,差异无统计学意义(P>0.05);治疗后,观察组患者疼痛评分低于对照组,肝功能指标优于对照组,差异有统计学意义(P<0.05)。结论在TACE的基础之上联合复方苦参,可以将原发性肝癌患者在手术后的并发症发生率降低,改善患者肝功能损伤的严重程度。展开更多
基金the National Natural Science Foundation of China(No.81774093)。
文摘Ulcerative colitis(UC)is a chronic refractory non-specific intestinal inflammatory disease that is difficult to be cured.The discovery of new ulcerative colitis-related metabolite biomarkers may help further understand UC and facilitate early diagnosis.It may also provide a basis for explaining the mechanism of drug action in the treatment of UC.Compound Sophorae Decoction(CSD)is an empirical formula used in the clinical treatment of UC.Although it is known to be efficacious,its mechanism of action in the treatment of UC is unclear.The purpose of this study was to investigate the changes in endogenous substances in UC rats and the effects of CSD on metabolic pathways using the metabonomics approach.Metabolomics studies in rats with UC and normal rats were performed using LC-MS/MS.Rats with UC induced using TNBS enema were used as the study models.Metabolic profiling and pathway analysis of biomarkers was performed using statistical and pathway enrichment analyses.36 screened potential biomarkers were found to be significantly different between the UC and the normal groups;it was also found that CSD could modulate the levels of these potential biomarkers.CSD was found to be efficacious in UC by regulating multiple metabolic pathways.
基金Supported by the Chinese National Natural Science Foundation (Immunomodulatory Mechanism of Bmsc Evs Mediated by Ephb2/Ephrin-b1 Pathway in Ulcerative Colitis and Synergistic Effect of Compound Sophorae decoction, No.81774093)。
文摘OBJECTIVE: To investigate how compound Sophorae decoction(CSD) works on rats' models of ulcerative colitis(UC) induced by 2,4,6-trinitrobenzenesulfonic acid solution(TNBS) by metabolomics studies of colon, liver, and kidney tissue extracts.METHODS: Rats with UC induced by TNBS enema were used as models in this study. Metabolic profiles of the three tissues were analyzed and pathway analysis of biomarkers was performed after CSD administration and further integration of metabolic networks.RESULTS: Thirteen biomarkers were screened from colon, liver, and kidney tissue extracts, and the levels of these substances were up-or down-regulated in the model group, but their levels were reversed after CSD administration. These biomarkers were mainly related to Phenylalanine, tyrosine and tryptophan biosynthesis, Phenylalanine metabolism, Glutathione metabolism, Arachidonic acid metabolism, Nicotinate and nicotinamide metabolism,Alanine, aspartate and glutamate metabolism.CONCLUSION: CSD could significantly ameliorate the symptoms of UC by regulating multiple metabolic pathways.
基金supported by grants from the Postdoctoral Science Foundation of China(No.2005037679)the National Natural Science Foundation of China(Grant No.30772878).
文摘The effects of compound Sophorae Flavescen-tis Jiechangrong capsule(CSFJC)on the expression of nuclear factor-κB p65(NF-κB p65)and signal transducer and activator of transcription 6(STAT6)in the intestinal mucosa of patients with ulcerative colitis and the possible mechanism were investigated.Eighteen patients with ulcerative colitis were randomly divided into a traditional Chinese medicine(TCM)group(n=11)treated by CSFJC and a western medicine(WM)group(n=7)treated by Sulfasalazine tablets.The treatment duration lasted eight weeks.Before and after the treatment,the symptoms and the physical signs were observed,and the routine stool test,the colonoscopy,and pathological examination were performed in the two groups.The expression levels of NF-κB p65 and STAT6 were detected by using immuno-histochemistry.The results showed that the total effective rate of the curative effectiveness in TCM and WM groups was 100%and 71.4%,respectively,and the total effective rate of colonic mucosa lesion in TCM and WM groups was 90.9%and 71.4%,respectively,with the differences being significant(all P<0.05).The total effective rate of syndromes of damp-heat blocking according to the TCM in TCM and WM groups was 90.9%and 71.4%,respectively.After the treatment,the expression of NF-κB p65 and STAT6 in the two groups was decreased,and the decrease of NF-κB p65 and STAT6 expression in TCM group was more significant than in WM group(P<0.05).It was concluded that CSFJC can inhibit the activation and expression of NF-κB p65 and STAT6 in the intestinal mucosa of patients with ulcerative colitis,which is a possible mechanism for CSFJC treating ulcerative colitis.
文摘目的探讨复方苦参联合肝动脉化疗栓塞术(chemoembolization of the hepatic artery,TACE)治疗原发性肝癌对患者术后不良反应及肝功能损伤的影响。方法随机选取2021年1月—2023年1月徐州市肿瘤医院接诊的原发性肝癌患者80例,利用随机数表法分为两组。对照组40例患者接受TACE进行治疗,观察组患者40例患者则采取TACE+复方苦参治疗。分析两组患者术后不良反应发生率、不良反应严重程度以及术后肝功能损伤程度。结果观察组患者术后不良反应发生率为10.00%,较对照组的30.00%更低,差异有统计学意义(χ^(2)=5.000,P=0.025);治疗前,两组患者疼痛评分和肝功能指标比较,差异无统计学意义(P>0.05);治疗后,观察组患者疼痛评分低于对照组,肝功能指标优于对照组,差异有统计学意义(P<0.05)。结论在TACE的基础之上联合复方苦参,可以将原发性肝癌患者在手术后的并发症发生率降低,改善患者肝功能损伤的严重程度。
