The theory of digital signal treatment and its application is a discipline with wide and promising prospects, having made its debut in the 1960s. During the period from the end of the 1950s to the 1970s, I was engaged...The theory of digital signal treatment and its application is a discipline with wide and promising prospects, having made its debut in the 1960s. During the period from the end of the 1950s to the 1970s, I was engaged in teaching and research work in the fields of electronics,digital circuits and numerical control and participated in the development of China’s first numerical machine. At the end of the 1970s.I went to the United States展开更多
In vivo fluorescence has a wide application in analyzing microalgae, including assessing phytoplankton biomass, rates of primary production and physiological status. This study describes a preliminary investigation on...In vivo fluorescence has a wide application in analyzing microalgae, including assessing phytoplankton biomass, rates of primary production and physiological status. This study describes a preliminary investigation on the joint application of the three kinds of fluorescence analysis in the physiological study of microalgae. Flow cytometry and fluorescence spectrometry were used to obtain the in vivo static fluorescence information of pigments, and a Pulsed-Amplitude-Modulation chlorophyll fluorometer was used to detect the dynamic fluorescence of chlorophyll. The validity of the joint application was proved by analyzing two labora- tory cultured Arctic microalgae, Pseudo-nitzschia delicatissima （Bacillariophyceae） and Thalassiosira sp. The higher value of minimum fluorescence yield in dark-adapted state （Fo）, actual photochemical efficiency of PSll （ФPSII）, and electron transport rate （ETR） exhibited positive results in a higher cell abundance and chlorophyll a content of P. delicatissima; whereas higher fl-carotene content of Thalassiosira sp. played an important role in the protection of photosynthesis.展开更多
文摘The theory of digital signal treatment and its application is a discipline with wide and promising prospects, having made its debut in the 1960s. During the period from the end of the 1950s to the 1970s, I was engaged in teaching and research work in the fields of electronics,digital circuits and numerical control and participated in the development of China’s first numerical machine. At the end of the 1970s.I went to the United States
基金financially supported by the National Natural Science Foundation of China (Grant no.41076130)the SOA Youth Marine Science Foundation (Grant no.2010116)the Open Research Foundation of Laboratory of Marine Ecosystem and Biogeochemistry,SOA (Grant no.LMEB200902)
文摘In vivo fluorescence has a wide application in analyzing microalgae, including assessing phytoplankton biomass, rates of primary production and physiological status. This study describes a preliminary investigation on the joint application of the three kinds of fluorescence analysis in the physiological study of microalgae. Flow cytometry and fluorescence spectrometry were used to obtain the in vivo static fluorescence information of pigments, and a Pulsed-Amplitude-Modulation chlorophyll fluorometer was used to detect the dynamic fluorescence of chlorophyll. The validity of the joint application was proved by analyzing two labora- tory cultured Arctic microalgae, Pseudo-nitzschia delicatissima （Bacillariophyceae） and Thalassiosira sp. The higher value of minimum fluorescence yield in dark-adapted state （Fo）, actual photochemical efficiency of PSll （ФPSII）, and electron transport rate （ETR） exhibited positive results in a higher cell abundance and chlorophyll a content of P. delicatissima; whereas higher fl-carotene content of Thalassiosira sp. played an important role in the protection of photosynthesis.
