The new spreading conchospores of Porphyra yezoensis, P. haitanensis and P. katadai var. hemiphylla are induced by N-Methy-N’-Nitro-N-Nitrosoguanidine (NG), a ⑴strong mutagen to induce the mutant of chromatophore. I...The new spreading conchospores of Porphyra yezoensis, P. haitanensis and P. katadai var. hemiphylla are induced by N-Methy-N’-Nitro-N-Nitrosoguanidine (NG), a ⑴strong mutagen to induce the mutant of chromatophore. It is showed that no mutants have ⑵been investigated in all control group. The conchospores of all the three species of Porphyra are easy to be induced by NG for they are in the stage of breeding, In the lowest dose (10 μg/mL) of this experiment, the mutant rate of P. yezoensis is up to 57.9%, that of P. haitanensis 30.7% and that of P. katadai var. hemiphylla ⑶51.7%. The mutant rate can not increase obviously by increasing the reagent concentration or prolonging the induction time. Within the scope of experiment, the inducement effects of three species of Porphyra are much similar, and the mutant rate is near 50%. The optimum induction concentration of NG is 10 μg/mL, and the optimum induction time is 30 minutes.展开更多
A heat-resistant Pyropia yezoensis strain YZ-4 was isolated from the regenerated blades of single somatic cells of the wild-type(WT)blades treated with 60Co-g-irradiation.When cultured at 23C and 24C,the survival an...A heat-resistant Pyropia yezoensis strain YZ-4 was isolated from the regenerated blades of single somatic cells of the wild-type(WT)blades treated with 60Co-g-irradiation.When cultured at 23C and 24C,the survival and division rates of the YZ-4 conchospores were significantly higher than those of WT(P<0.01).After 16 days in culture the survival rates of YZ-4 conchospores were 71.2%and 60.8%at 23C and 24C,respectively,and the division rates of the surviving conchospores were 100%in both cases.Under the same culture conditions,the survival rates of WT conchospores were only 16.9%and 11.5%,and the division rate 99.8%and 81.8%respectively.The resistance of 50-day-aged F1 gametophytic blades of YZ-4 to the temperature rebound was also significantly higher than those of WT(P<0.01).After 30 days in culture at 18C,23C and 24C,the mean blade length increased by 8.5,4.3 and 2.3 times in YZ-4 and was only 3,0.6 and 0.4 times in WT,respectively.The mean blade weight increased 191.9,85.4 and 66.2 times in YZ-4,but only 78.2,7.8 and 4.6 times in WT.On the 15th day WT blades began to decay,but no decay was observed in YZ-4 blades even after 45 days in culture at 23C and 24C.The results obtained indicate that YZ-4 has a higher resistance to higher temperature insults than WT and it might be of value for commercial production of P.yezoensis.展开更多
基金Supported by NSFCC39770593 EMBLC CAS and Applied Fundamental Project of Jiangsu Province (BJ95182). National Key Fundamental Research Program (G1999012009).
文摘The new spreading conchospores of Porphyra yezoensis, P. haitanensis and P. katadai var. hemiphylla are induced by N-Methy-N’-Nitro-N-Nitrosoguanidine (NG), a ⑴strong mutagen to induce the mutant of chromatophore. It is showed that no mutants have ⑵been investigated in all control group. The conchospores of all the three species of Porphyra are easy to be induced by NG for they are in the stage of breeding, In the lowest dose (10 μg/mL) of this experiment, the mutant rate of P. yezoensis is up to 57.9%, that of P. haitanensis 30.7% and that of P. katadai var. hemiphylla ⑶51.7%. The mutant rate can not increase obviously by increasing the reagent concentration or prolonging the induction time. Within the scope of experiment, the inducement effects of three species of Porphyra are much similar, and the mutant rate is near 50%. The optimum induction concentration of NG is 10 μg/mL, and the optimum induction time is 30 minutes.
基金the National High Technology Research&Development Program of China(“863”Program)(Grant No.2006AA10A413)the National Natural Science Foundation of China(Grant No.31072208)+2 种基金the Shanghai Natural Science Foundation(Grant No.05RZ14110)Science and Technology Commission of Shanghai Municipality(Grant No.07XD14028)Shanghai Municipal Education Commission(Project No.J50701).
文摘A heat-resistant Pyropia yezoensis strain YZ-4 was isolated from the regenerated blades of single somatic cells of the wild-type(WT)blades treated with 60Co-g-irradiation.When cultured at 23C and 24C,the survival and division rates of the YZ-4 conchospores were significantly higher than those of WT(P<0.01).After 16 days in culture the survival rates of YZ-4 conchospores were 71.2%and 60.8%at 23C and 24C,respectively,and the division rates of the surviving conchospores were 100%in both cases.Under the same culture conditions,the survival rates of WT conchospores were only 16.9%and 11.5%,and the division rate 99.8%and 81.8%respectively.The resistance of 50-day-aged F1 gametophytic blades of YZ-4 to the temperature rebound was also significantly higher than those of WT(P<0.01).After 30 days in culture at 18C,23C and 24C,the mean blade length increased by 8.5,4.3 and 2.3 times in YZ-4 and was only 3,0.6 and 0.4 times in WT,respectively.The mean blade weight increased 191.9,85.4 and 66.2 times in YZ-4,but only 78.2,7.8 and 4.6 times in WT.On the 15th day WT blades began to decay,but no decay was observed in YZ-4 blades even after 45 days in culture at 23C and 24C.The results obtained indicate that YZ-4 has a higher resistance to higher temperature insults than WT and it might be of value for commercial production of P.yezoensis.
