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Study on The Method of Quantitative Analysis of Serum Ferritin and Soluble Transferrin Receptor with Protein Microarray Technology 被引量:4
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作者 YIN Ji Yong SUN Jing +2 位作者 HUANG Jian LI Wen Xian HUO Jun Sheng 《Biomedical and Environmental Sciences》 SCIE CAS CSCD 2012年第4期430-439,共10页
Objective To establish and evaluate a protein serum ferritin (SF) and soluble transferrin receptor microarray method for combined measurement of (sTfR). Methods Microarrayer was used to print both anti-SF antibodi... Objective To establish and evaluate a protein serum ferritin (SF) and soluble transferrin receptor microarray method for combined measurement of (sTfR). Methods Microarrayer was used to print both anti-SF antibodies I and anti-sTfR antibodies I on each protein microarray. Anti-SF antibodies II and anti-sTfR antibodies II were used as detection antibodies and goat antibodies coupled to Cy3 were used as antibodies Ill. The detection conditions of the quantitative analysis method for simultaneous measurement of SF and sTfR with protein microarray were optimized and evaluated. The protein microarray was compared with commercially available traditional tests with 26 serum samples. Results By comparison experiment, mouse monoclonal antibodies were chosen as the probes and contact printing was chosen as the printing method. The concentrations of SF and sTfR probes were 0.5 mg/mL and 0.5 mg/mL respectively, while those of SF and sTfR detection antibodies were 5 μg/mL and 0.36 μg/mL respectively. Intra- and inter-assay variability was between 3.26% and 18.38% for all tests. The regression coefficients comparing protein microarray with traditional test assays were better than 0.81 for SF and sTfR. Conclusion The present study has established a protein microarray method for combined measurement of SF and sTfR. 展开更多
关键词 Protein microarray OPTIMIZATION Combined measurement conditions Serum ferritin Soluble transferrin receptor.
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An experimental research of fluid piston oscillation in square moon pool under the wave-flow combined conditions
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作者 Kang Zhuang Yao Xiongliang 《Engineering Sciences》 EI 2011年第1期19-23,共5页
The article studies the oscillation features of the water in the square moon pool under the circumstances of wave-flow combined conditions. Comparing with the results of experiments of the square moon pool, a series o... The article studies the oscillation features of the water in the square moon pool under the circumstances of wave-flow combined conditions. Comparing with the results of experiments of the square moon pool, a series of studies reveal that the water piston oscillation phenomenon is quite similar to that of the circular one. Two types of oscillation are exhibited under different incoming wave periods, i.e. self-excited oscillation and forced oscillation. The difference between the circular and square moon pool is that the attack angle makes greater influences on the square one. "Beating phenomenon" is also found in the square moon pool which is not mentioned of the circular one. 展开更多
关键词 square moon pool piston oscillation wave-flow combined condition beating phenomenon
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