The temperature-dependence of infraeeliular free caleimn (Ca) was investigated in mdo-1 loaded ventricular myocytes from the ral, a non-hibernator, and from the ground squirrel, a hibernator. The dissociation constant...The temperature-dependence of infraeeliular free caleimn (Ca) was investigated in mdo-1 loaded ventricular myocytes from the ral, a non-hibernator, and from the ground squirrel, a hibernator. The dissociation constant of indo-l at different temperatures was calibrated both al pll-tat and at @-stat . and the result demonstrated that the @-stat ralibration should be prettrred . Analysis of the fluoreseent image showed a striking increase of Ca2 as well as spontaneous caleiuni waves in ral cells, indicating an overloaded cakuum. In contrast, cardiac myocytes of the ground sqnirraf were found to keep a constant (Ca2+) without caleium overload regardless of temperature variation. It is be-lieved that understanding of the mechanisms underlying the interccllular caleima homeostasis of hibrernators may lead to solutions of some medical questions .展开更多
With the rapid development of Mg alloys,deeper understanding to the thermodynamic and diffusional kinetic behavior of intermetallic compounds(IMCs)is important for studying the effect of alloying elements to the micro...With the rapid development of Mg alloys,deeper understanding to the thermodynamic and diffusional kinetic behavior of intermetallic compounds(IMCs)is important for studying the effect of alloying elements to the microstructure evolution.Specially,a systematic quantitative investigation on the diffusional growth of IMCs is of great necessity.However,the works studying the elemental diffusion behaviors of multiple-element IMCs are rare in magnesium alloy systems.The current work takes the ternary Mg-Al-Zn system as research target,and combines the diffusion couple technique,phase stability diagrams,in-situ observation technique and numerical inverse method to investigate the temperature-dependent kinetic coefficients.The parabolic growth constant(PGC)and interdiffusion coefficients for Mg solid-solution phase andγ-Mg_(17)Al_(12),β-Mg_(2)Al_(3),ε-Mg_(23)Al_(30),MgZn_(2),Mg_(2)Zn_(3),τ-Mg_(32)(Zn,Al)49 andφ-Mg_(5)Zn_(2)Al_(2) IMCs in the Mg-Al-Zn alloy system are determined.By comparing the current experimental with calculation results,the rate-controlling factor of the temperature-dependent diffusion growth ofφ,τandεternary IMCs in the Mg-Al-Zn system is further discussed in detail.展开更多
介绍了宝钢研究院共焦激光扫描显微镜(Confocal Laser Scann ing M icroscopy,CLSM)的结构及其工作原理,在此基础上通过Fe-C二元合金凝固、奥氏体不锈钢凝固、低碳钢中高温铁素体(δ)奥氏体(γ)相变界面稳定性、AISI304不锈钢加热过程...介绍了宝钢研究院共焦激光扫描显微镜(Confocal Laser Scann ing M icroscopy,CLSM)的结构及其工作原理,在此基础上通过Fe-C二元合金凝固、奥氏体不锈钢凝固、低碳钢中高温铁素体(δ)奥氏体(γ)相变界面稳定性、AISI304不锈钢加热过程中δ相的形核与生长、AISI304不锈钢冷却过程中δ→γ相变等典型实例描述了CLSM在钢铁相变原位观察中的应用情况。表明CLSM在研究钢铁材料的凝固、固态相变、夹杂运动等方面具有无与伦比的优势与应用前景。展开更多
Plasmid pVK1001 which carried the gfp gene of GFPmut2, a mutant of GFP, was introduced into Azospirillum brasilense Yu62 by electroporation. Maize seedlings were inoculated with the GFP-labelled baeteria and grown gno...Plasmid pVK1001 which carried the gfp gene of GFPmut2, a mutant of GFP, was introduced into Azospirillum brasilense Yu62 by electroporation. Maize seedlings were inoculated with the GFP-labelled baeteria and grown gnotobiotically in flask with semi-solid agar medium. Observations were performed with confocal laser scanning microscopy (CLSM) and electron microscopy, respectively, at 8 d and 12 d after inoculation. Confocal laser scanning microscopy showed that A. brasilense Yu62 could penetrate into the cortex tissue, colonizing in the intercellular spaces of the parenchyma cells of the cortex tissue. Transmission and scanning electron microscopy (TEM) showed that the majority of the bacteria colonized on the root surface and only a minority of them resided in the root interior.展开更多
The characters and ultrastructure of the intercellular connection were revealed in the outer epidermis of the garlic clove sheath by means of fluorescent probe TRITC_Phalloidin (TRITC_Ph) labeling combined with confoc...The characters and ultrastructure of the intercellular connection were revealed in the outer epidermis of the garlic clove sheath by means of fluorescent probe TRITC_Phalloidin (TRITC_Ph) labeling combined with confocal laser scanning microscopy (CLSM), immuno_gold labeling and transmission electron microscopy. These results show that transcellular channel is a complex of rod_like cytoplasm channel and grouped plasmodesmata (PDs) in pit. The former remains a portion of the cell protoplast. The diameter of PD is normally 60-70 nm. The PDs are the real intercellular symplasmic connections of the cells. The transcellular fibers labeled with the TRITC_Ph obviously become narrow in the primary pit fields, which is the same as the characters observed under the electron microscope. The bright fluorescent spot in the primary wall reflects the grouped PDs in pit, and hence the presence of F_actin in the PDs can be confirmed. In immuno_gold labeling experiment, a lot of gold particles were massively distributed in the rod_like cytoplasm channel and grouped PDs. The result provides effective support that these fluorescent filaments possibly are the existing form of F_actin.展开更多
By means of paraformaldehyde fixation, Triton X100 extraction and TRITCphalloidin staining, the presence and distribution patterns of Factin in the outer epidermal cells of the garlic （Allium sativum L.） sheath were...By means of paraformaldehyde fixation, Triton X100 extraction and TRITCphalloidin staining, the presence and distribution patterns of Factin in the outer epidermal cells of the garlic （Allium sativum L.） sheath were studied with fluorescence probe technique and confocal laser scanning microscopy. There were a lot of actin filaments （AFs） impenetrate the cell wall, but the AFs with red fluorescence were absent when the cells were treated with cytochalasin D before fixation; the same result was obtained when the cells were treated with unlabeled phalloidin. These results indicate the presence of Factin in the intercellular channels and that it is related to the plasmodesmata and intercellular trafficking of macromolecules.展开更多
基金Project supported by the National Natural Science Foundation of China(Grant No. 39670276).
文摘The temperature-dependence of infraeeliular free caleimn (Ca) was investigated in mdo-1 loaded ventricular myocytes from the ral, a non-hibernator, and from the ground squirrel, a hibernator. The dissociation constant of indo-l at different temperatures was calibrated both al pll-tat and at @-stat . and the result demonstrated that the @-stat ralibration should be prettrred . Analysis of the fluoreseent image showed a striking increase of Ca2 as well as spontaneous caleiuni waves in ral cells, indicating an overloaded cakuum. In contrast, cardiac myocytes of the ground sqnirraf were found to keep a constant (Ca2+) without caleium overload regardless of temperature variation. It is be-lieved that understanding of the mechanisms underlying the interccllular caleima homeostasis of hibrernators may lead to solutions of some medical questions .
基金funded by the National Natural Science Foundation of China(No.51801116 and 52001176)the Shandong Province Key Research and Development Plan(No.2019GHZ019 and 2021SFGC1001)the Youth Innovation and Technology Support Program of Shandong Provincial Colleges and Universities(No.2020KJA002).
文摘With the rapid development of Mg alloys,deeper understanding to the thermodynamic and diffusional kinetic behavior of intermetallic compounds(IMCs)is important for studying the effect of alloying elements to the microstructure evolution.Specially,a systematic quantitative investigation on the diffusional growth of IMCs is of great necessity.However,the works studying the elemental diffusion behaviors of multiple-element IMCs are rare in magnesium alloy systems.The current work takes the ternary Mg-Al-Zn system as research target,and combines the diffusion couple technique,phase stability diagrams,in-situ observation technique and numerical inverse method to investigate the temperature-dependent kinetic coefficients.The parabolic growth constant(PGC)and interdiffusion coefficients for Mg solid-solution phase andγ-Mg_(17)Al_(12),β-Mg_(2)Al_(3),ε-Mg_(23)Al_(30),MgZn_(2),Mg_(2)Zn_(3),τ-Mg_(32)(Zn,Al)49 andφ-Mg_(5)Zn_(2)Al_(2) IMCs in the Mg-Al-Zn alloy system are determined.By comparing the current experimental with calculation results,the rate-controlling factor of the temperature-dependent diffusion growth ofφ,τandεternary IMCs in the Mg-Al-Zn system is further discussed in detail.
文摘介绍了宝钢研究院共焦激光扫描显微镜(Confocal Laser Scann ing M icroscopy,CLSM)的结构及其工作原理,在此基础上通过Fe-C二元合金凝固、奥氏体不锈钢凝固、低碳钢中高温铁素体(δ)奥氏体(γ)相变界面稳定性、AISI304不锈钢加热过程中δ相的形核与生长、AISI304不锈钢冷却过程中δ→γ相变等典型实例描述了CLSM在钢铁相变原位观察中的应用情况。表明CLSM在研究钢铁材料的凝固、固态相变、夹杂运动等方面具有无与伦比的优势与应用前景。
文摘Plasmid pVK1001 which carried the gfp gene of GFPmut2, a mutant of GFP, was introduced into Azospirillum brasilense Yu62 by electroporation. Maize seedlings were inoculated with the GFP-labelled baeteria and grown gnotobiotically in flask with semi-solid agar medium. Observations were performed with confocal laser scanning microscopy (CLSM) and electron microscopy, respectively, at 8 d and 12 d after inoculation. Confocal laser scanning microscopy showed that A. brasilense Yu62 could penetrate into the cortex tissue, colonizing in the intercellular spaces of the parenchyma cells of the cortex tissue. Transmission and scanning electron microscopy (TEM) showed that the majority of the bacteria colonized on the root surface and only a minority of them resided in the root interior.
文摘The characters and ultrastructure of the intercellular connection were revealed in the outer epidermis of the garlic clove sheath by means of fluorescent probe TRITC_Phalloidin (TRITC_Ph) labeling combined with confocal laser scanning microscopy (CLSM), immuno_gold labeling and transmission electron microscopy. These results show that transcellular channel is a complex of rod_like cytoplasm channel and grouped plasmodesmata (PDs) in pit. The former remains a portion of the cell protoplast. The diameter of PD is normally 60-70 nm. The PDs are the real intercellular symplasmic connections of the cells. The transcellular fibers labeled with the TRITC_Ph obviously become narrow in the primary pit fields, which is the same as the characters observed under the electron microscope. The bright fluorescent spot in the primary wall reflects the grouped PDs in pit, and hence the presence of F_actin in the PDs can be confirmed. In immuno_gold labeling experiment, a lot of gold particles were massively distributed in the rod_like cytoplasm channel and grouped PDs. The result provides effective support that these fluorescent filaments possibly are the existing form of F_actin.
文摘By means of paraformaldehyde fixation, Triton X100 extraction and TRITCphalloidin staining, the presence and distribution patterns of Factin in the outer epidermal cells of the garlic （Allium sativum L.） sheath were studied with fluorescence probe technique and confocal laser scanning microscopy. There were a lot of actin filaments （AFs） impenetrate the cell wall, but the AFs with red fluorescence were absent when the cells were treated with cytochalasin D before fixation; the same result was obtained when the cells were treated with unlabeled phalloidin. These results indicate the presence of Factin in the intercellular channels and that it is related to the plasmodesmata and intercellular trafficking of macromolecules.
