Essential hypertension（EH） is affected by both genetic and environmental factors.The polymorphism of connexin（Cx） genes is found associated with the development of hypertension.However,the association of the polym...Essential hypertension（EH） is affected by both genetic and environmental factors.The polymorphism of connexin（Cx） genes is found associated with the development of hypertension.However,the association of the polymorphism of Cxs with EH has not been investigated.This study aimed to investigate the association of the polymorphism of connexin（Cx） genes Cx37,Cx40,and Cx43 with EH in Kazak and Han Chinese in Xinjiang,China.Polymerase chain reaction-restriction fragment length polymorphism（PCR-RFLP） method and matrix-assisted laser desorption ionization time-of-flight mass spectrometry（MALDI-TOF-MS） were used to analyze the polymorphism of Cx genes in Kazak and Han EH patients as well as their normotensive controls.The results showed that there were no significant differences in the frequencies of different three genotypes（A/A,A/G,and G/G） and A and G alleles of Cx40 rs35594137 and rs11552588 between EH patients and normotensive controls.However,in Kazak EH patients,the frequencies of three genotypes（A/A,A/G,and G/G） of Cx37 rs1630310 were 24.8%,47.2% and 28.0%,respectively,which were significantly different from those in Han EH patients.In Han EH patients,the frequencies of the three genotypes（C/C,C/G and G/G） of Cx43 rs1925223 were 6.4%,35.6% and 58.0%,respectively.Frequencies of the other four genotypes had no statistical differences among Kazak and Han EH patients and their normotensive controls.These results suggest polymorphisms of Cx37 rs1630310 and Cx43 rs1925223 genes may be associated with the pathogenesis of EH.Carrying Cx37 rs1630310-A or Cx43 rs1925223-G genotypes may protect against the development of EH.展开更多
Objective: To detect which members in the connexin gene family are expressed in nasopharyngeal carcinoma (NPC) cell line HNE1, and the mechanism by which those genes are specifically switched on and off during retinoi...Objective: To detect which members in the connexin gene family are expressed in nasopharyngeal carcinoma (NPC) cell line HNE1, and the mechanism by which those genes are specifically switched on and off during retinoic acid (RA) induction. Methods: Establishing the cell growth curves of NPC cells. Observing the effect of RA on connexin genes by Northern hybridization. Results: Two genes Cx46 and Cx37, belonging to the connexin gene family, were expressed in HNE1. The down-regulation of Cx46 and Cx37, up-regulation of RARa and growth inhibition was observed in HNE1 after exposure to RA. The gene expression and cell growth in HNE1 cells was restored after removal of RA. Conclusion: Two members of the connexin gene family: Cx37 and Cx46 were expressed in HNE1 cells, RA can inhibit the expression of those tow genes mediated by RARa, and the effects of RA on HNE1 are reversible.展开更多
AIM To investigate the significance andmechanism of cx32 mRNA,cx43 mRNA and theirproteins in hepatocarcinogenesis.METHODS Sixty-one cases of HCC and 14cases of normal liver tissues were detected byimmunohistochemical ...AIM To investigate the significance andmechanism of cx32 mRNA,cx43 mRNA and theirproteins in hepatocarcinogenesis.METHODS Sixty-one cases of HCC and 14cases of normal liver tissues were detected byimmunohistochemical and in situ hybridization(ISH)methods.RESULTS In HCC grades Ⅰ,Ⅱ,Ⅲ and normalliver tissues,the positive rates of Cx32 proteinwere 55.6%,42.1%,18.2% and 92.9%,respectively.The detection rates of Cx43 proteinwere 44.4%,26.3%,12.1% and 78.6%,respectively.There was significant difference inCx32 and Cx43 protein between HCC and normalliver tissues(P【0.01).ISH the positive rates ofcx32 mRNA shown by ISH in HCC grades Ⅰ,Ⅱ,Ⅲ and normal liver tissues were 88.9%,84.2%,87.9% and 92.9%,respectively.Those of cx43mRNA were 77.8%,78.6%,78.8% and 85.7%,respectively.There was no statistical differencein the positive rates of cx32 mRNA and cx43mRNA between HCC and normal liver tissue(P】0.05).CONCLUSION The aberrant location of Cx32and Cx43 proteins could be responsible forprogression of hepatocarcinogenesis,and thedefect of cx genes in post-translationalprocessing might be the possible mechanism.展开更多
Connexin-43 (Cx43) expression in prostate cancer (PCa) cells and the potency of gap junctional intercellular communication (GJIC) in the cells were investigated, with an attempt to elu- cidate the reason why the so-ca...Connexin-43 (Cx43) expression in prostate cancer (PCa) cells and the potency of gap junctional intercellular communication (GJIC) in the cells were investigated, with an attempt to elu- cidate the reason why the so-called 'bystander effect' mediated by thymidine kinase (TK) suicide gene therapy on PCa cells is not of significance and to explore the role of GJIC in PCa carcinogenesis. mRNA and protein expression of Cx43 in a PCa cell line PC-3m was detected by re- verse-transcription polymerase chain reaction (RT-PCR) and strapt-avidin-biotin-enzyme complex (SABC) immunohistochemical staining, and inherent GJIC of PC-3m cells was assayed by scrape-loading and dye transfer (SLDT) assay. The expression of Cx43 in human normal and malig- nant prostate tissues was determined by SABC immunohistochemistry as well. It was found that Cx43 mRNA and protein expression in PC-3m cells was slightly reduced as compared with positive controls and the location of Cx43 protein was aberrant in cytoplasm rather than on membrane. As- sessment of paraffin sections demonstrated that the expression of Cx43 protein in PCa cells was ab- normally located and markedly diminished as compared with normal prostatic epithelial ones, dis- playing a negative correlation to the pathological grade (χ2=4.025, P<0.05). Additionally, capacity of inherent GJIC in PC-3m cells was disrupted, which was semi-quantified as (+) or (-). It was indi- cated that both down-regulated expression of Cx43 mRNA and aberrant location of Cx43 protein par- ticipated in the mechanisms leading to deficient GJIC in PC-3m cells. Lack of efficient GJIC is a molecular event, which may contribute not only to limited extent of 'bystander effect', but also to initiation and progression of prostatic neoplasm.展开更多
Genetic defects are one of the most important etiologies of severe to profound sensorineural hearing loss and play an important role in determining cochlear implantation outcomes.While the pathogenic mutation types of...Genetic defects are one of the most important etiologies of severe to profound sensorineural hearing loss and play an important role in determining cochlear implantation outcomes.While the pathogenic mutation types of a number of deafness genes have been cloned,the pathogenesis mechanisms and their relationship to the outcomes of cochlear implantation remain a hot research area.The auditory performance is considered to be affected by the etiology of hearing loss and the number of surviving spiral ganglion cells,as well as others.Current research advances in cochlear implantation for hereditary deafness,especially the relationship among clinic-types,genotypes and outcomes of cochlear implantation,will be discussed in this review.展开更多
背景与目的:原位癌中间隙连接蛋白(connexin,CX)介导的细胞间隙连接通讯(gap junction intercellular com m unication,GJIC)是相邻细胞之间直接通讯的惟一方式,CX表达的变化以及GJIC功能的改变可能在宫颈组织癌变过程中起一定的作用并...背景与目的:原位癌中间隙连接蛋白(connexin,CX)介导的细胞间隙连接通讯(gap junction intercellular com m unication,GJIC)是相邻细胞之间直接通讯的惟一方式,CX表达的变化以及GJIC功能的改变可能在宫颈组织癌变过程中起一定的作用并对原位癌发生发展有重要影响。本研究旨在检测宫颈正常鳞状上皮、不典型增生上皮、原位癌和浸润性鳞癌这个连续癌变模式中CX m RNA和蛋白质表达的变化,并探讨CX表达的变化与宫颈原位癌发生、发展的相关性。方法:采用免疫组化和原位杂交技术检测30例宫颈原位癌石蜡切片中CX43和CX26m RNA和蛋白质表达情况,采用免疫组化检测Ki-67蛋白的表达,并以宫颈30例正常鳞状上皮、22例不典型增生上皮和26例浸润性鳞癌为对照。结果:(1)在正常鳞状上皮、不典型增生上皮、原位癌和浸润性鳞癌中,CX43蛋白质阳性率依次为83%、55%、50%、30%;CX43m RNA阳性率依次为97%、64%、58%、46%;CX26m RNA阳性率依次为93%、68%、55%、42%;可以看出CX43(m RNA、蛋白质)和CX26(m RNA)阳性率逐渐降低。原位癌组CX阳性率明显低于正常鳞状上皮组,两组之间差异有显著性(P<0.05);原位癌组内,CX表达以弱阳性为主,阳性程度明显低于正常鳞状上皮;原位癌与正常鳞状上皮直接相邻区域,CX表达是明显减弱或消失的。(2)展开更多
基金supported by grants from National Basic Research Program of China(No.2012CB526600)National Natural Science Foundation of China(No.81560081,No.31460264 and No.81560175)
文摘Essential hypertension（EH） is affected by both genetic and environmental factors.The polymorphism of connexin（Cx） genes is found associated with the development of hypertension.However,the association of the polymorphism of Cxs with EH has not been investigated.This study aimed to investigate the association of the polymorphism of connexin（Cx） genes Cx37,Cx40,and Cx43 with EH in Kazak and Han Chinese in Xinjiang,China.Polymerase chain reaction-restriction fragment length polymorphism（PCR-RFLP） method and matrix-assisted laser desorption ionization time-of-flight mass spectrometry（MALDI-TOF-MS） were used to analyze the polymorphism of Cx genes in Kazak and Han EH patients as well as their normotensive controls.The results showed that there were no significant differences in the frequencies of different three genotypes（A/A,A/G,and G/G） and A and G alleles of Cx40 rs35594137 and rs11552588 between EH patients and normotensive controls.However,in Kazak EH patients,the frequencies of three genotypes（A/A,A/G,and G/G） of Cx37 rs1630310 were 24.8%,47.2% and 28.0%,respectively,which were significantly different from those in Han EH patients.In Han EH patients,the frequencies of the three genotypes（C/C,C/G and G/G） of Cx43 rs1925223 were 6.4%,35.6% and 58.0%,respectively.Frequencies of the other four genotypes had no statistical differences among Kazak and Han EH patients and their normotensive controls.These results suggest polymorphisms of Cx37 rs1630310 and Cx43 rs1925223 genes may be associated with the pathogenesis of EH.Carrying Cx37 rs1630310-A or Cx43 rs1925223-G genotypes may protect against the development of EH.
文摘Objective: To detect which members in the connexin gene family are expressed in nasopharyngeal carcinoma (NPC) cell line HNE1, and the mechanism by which those genes are specifically switched on and off during retinoic acid (RA) induction. Methods: Establishing the cell growth curves of NPC cells. Observing the effect of RA on connexin genes by Northern hybridization. Results: Two genes Cx46 and Cx37, belonging to the connexin gene family, were expressed in HNE1. The down-regulation of Cx46 and Cx37, up-regulation of RARa and growth inhibition was observed in HNE1 after exposure to RA. The gene expression and cell growth in HNE1 cells was restored after removal of RA. Conclusion: Two members of the connexin gene family: Cx37 and Cx46 were expressed in HNE1 cells, RA can inhibit the expression of those tow genes mediated by RARa, and the effects of RA on HNE1 are reversible.
文摘AIM To investigate the significance andmechanism of cx32 mRNA,cx43 mRNA and theirproteins in hepatocarcinogenesis.METHODS Sixty-one cases of HCC and 14cases of normal liver tissues were detected byimmunohistochemical and in situ hybridization(ISH)methods.RESULTS In HCC grades Ⅰ,Ⅱ,Ⅲ and normalliver tissues,the positive rates of Cx32 proteinwere 55.6%,42.1%,18.2% and 92.9%,respectively.The detection rates of Cx43 proteinwere 44.4%,26.3%,12.1% and 78.6%,respectively.There was significant difference inCx32 and Cx43 protein between HCC and normalliver tissues(P【0.01).ISH the positive rates ofcx32 mRNA shown by ISH in HCC grades Ⅰ,Ⅱ,Ⅲ and normal liver tissues were 88.9%,84.2%,87.9% and 92.9%,respectively.Those of cx43mRNA were 77.8%,78.6%,78.8% and 85.7%,respectively.There was no statistical differencein the positive rates of cx32 mRNA and cx43mRNA between HCC and normal liver tissue(P】0.05).CONCLUSION The aberrant location of Cx32and Cx43 proteins could be responsible forprogression of hepatocarcinogenesis,and thedefect of cx genes in post-translationalprocessing might be the possible mechanism.
文摘Connexin-43 (Cx43) expression in prostate cancer (PCa) cells and the potency of gap junctional intercellular communication (GJIC) in the cells were investigated, with an attempt to elu- cidate the reason why the so-called 'bystander effect' mediated by thymidine kinase (TK) suicide gene therapy on PCa cells is not of significance and to explore the role of GJIC in PCa carcinogenesis. mRNA and protein expression of Cx43 in a PCa cell line PC-3m was detected by re- verse-transcription polymerase chain reaction (RT-PCR) and strapt-avidin-biotin-enzyme complex (SABC) immunohistochemical staining, and inherent GJIC of PC-3m cells was assayed by scrape-loading and dye transfer (SLDT) assay. The expression of Cx43 in human normal and malig- nant prostate tissues was determined by SABC immunohistochemistry as well. It was found that Cx43 mRNA and protein expression in PC-3m cells was slightly reduced as compared with positive controls and the location of Cx43 protein was aberrant in cytoplasm rather than on membrane. As- sessment of paraffin sections demonstrated that the expression of Cx43 protein in PCa cells was ab- normally located and markedly diminished as compared with normal prostatic epithelial ones, dis- playing a negative correlation to the pathological grade (χ2=4.025, P<0.05). Additionally, capacity of inherent GJIC in PC-3m cells was disrupted, which was semi-quantified as (+) or (-). It was indi- cated that both down-regulated expression of Cx43 mRNA and aberrant location of Cx43 protein par- ticipated in the mechanisms leading to deficient GJIC in PC-3m cells. Lack of efficient GJIC is a molecular event, which may contribute not only to limited extent of 'bystander effect', but also to initiation and progression of prostatic neoplasm.
文摘Genetic defects are one of the most important etiologies of severe to profound sensorineural hearing loss and play an important role in determining cochlear implantation outcomes.While the pathogenic mutation types of a number of deafness genes have been cloned,the pathogenesis mechanisms and their relationship to the outcomes of cochlear implantation remain a hot research area.The auditory performance is considered to be affected by the etiology of hearing loss and the number of surviving spiral ganglion cells,as well as others.Current research advances in cochlear implantation for hereditary deafness,especially the relationship among clinic-types,genotypes and outcomes of cochlear implantation,will be discussed in this review.
文摘背景与目的:原位癌中间隙连接蛋白(connexin,CX)介导的细胞间隙连接通讯(gap junction intercellular com m unication,GJIC)是相邻细胞之间直接通讯的惟一方式,CX表达的变化以及GJIC功能的改变可能在宫颈组织癌变过程中起一定的作用并对原位癌发生发展有重要影响。本研究旨在检测宫颈正常鳞状上皮、不典型增生上皮、原位癌和浸润性鳞癌这个连续癌变模式中CX m RNA和蛋白质表达的变化,并探讨CX表达的变化与宫颈原位癌发生、发展的相关性。方法:采用免疫组化和原位杂交技术检测30例宫颈原位癌石蜡切片中CX43和CX26m RNA和蛋白质表达情况,采用免疫组化检测Ki-67蛋白的表达,并以宫颈30例正常鳞状上皮、22例不典型增生上皮和26例浸润性鳞癌为对照。结果:(1)在正常鳞状上皮、不典型增生上皮、原位癌和浸润性鳞癌中,CX43蛋白质阳性率依次为83%、55%、50%、30%;CX43m RNA阳性率依次为97%、64%、58%、46%;CX26m RNA阳性率依次为93%、68%、55%、42%;可以看出CX43(m RNA、蛋白质)和CX26(m RNA)阳性率逐渐降低。原位癌组CX阳性率明显低于正常鳞状上皮组,两组之间差异有显著性(P<0.05);原位癌组内,CX表达以弱阳性为主,阳性程度明显低于正常鳞状上皮;原位癌与正常鳞状上皮直接相邻区域,CX表达是明显减弱或消失的。(2)
