Artificial animals are increasingly used as conspecific stimuli in animal behavior research.However,researchers often have an incomplete understanding of how the species under study perceives conspecifics,and,he...Artificial animals are increasingly used as conspecific stimuli in animal behavior research.However,researchers often have an incomplete understanding of how the species under study perceives conspecifics,and,hence,which features are needed for a stimulus to be perceived appropriately.To investigate the features that bearded dragons(Pogona vitticeps)attend to,we measured their lateralized eye use when assessing a successive range of stimuli.These ranged through several stages of realism in artificial conspecifics,to see how features such as color,the presence of eyes,body shape and motion influence behavior.We found differences in lateralized eye use depending on the sex of the observing bearded dragon and the artificial conspecific,as well as the artificial conspecific’s behavior.Therefore,this approach can inform the design of robotic animals that elicit biologically-meaningful responses in live animals.展开更多
Taxonomic uncertainties of rare species often hinder effective prioritization for conservation.One such taxonomic uncertainty is the 90-year-old enigma of Fagus chienii.F.chienii was previously only known from the typ...Taxonomic uncertainties of rare species often hinder effective prioritization for conservation.One such taxonomic uncertainty is the 90-year-old enigma of Fagus chienii.F.chienii was previously only known from the type specimens collected in 1935 in Pingwu County of Sichuan Province,China,and has long been thought to be on the verge of extinction.However,morphological similarities to closely related Fagus species have led many to question the taxonomic status of F.chienii.To clarify this taxonomic uncertainty,we used the newly collected samples to reconstruct a molecular phylogeny of Chinese Fagus species against the phylogenetic backbone of the whole genus using seven nuclear genes.In addition,we examined nine morphological characters to determine whether F.chienii is morphologically distinct from its putatively closest relatives(F.hayatae,F.longipetiolata,and F.lucida).Both morphological and phylogenetic analyses indicated that F.chienii is conspecific with F.hayatae.We recommended that F.chienii should not be treated as a separate species in conservation management.However,conservation strategies such as in situ protection and ex situ germplasm preservation should be adopted to prevent the peculiar“F.chienii”population from extinction.展开更多
Conspecific negative density dependence(CNDD)is a potentially important mechanism in maintaining species diversity.While previous evidence showed habitat heterogeneity and species’dispersal modes affect the strength ...Conspecific negative density dependence(CNDD)is a potentially important mechanism in maintaining species diversity.While previous evidence showed habitat heterogeneity and species’dispersal modes affect the strength of CNDD at early life stages of trees(e.g.,seedlings),it remains unclear how they affect the strength of CNDD at later life stages.We examined the degree of spatial aggregation between saplings and trees for species dispersed by wind and gravity in four topographic habitats within a 25-ha temperate forest dynamic plot in the Qinling Mountains of central China.We used the replicated spatial point pattern(RSPP)analysis and bivariate paircorrelation function(PCF)to detect the spatial distribution of saplings around trees at two scales,15 and 50 m,respectively.Although the signal was not apparent across the whole study region(or 25-ha),it is distinct on isolated areas with specific characteristics,suggesting that these characteristics could be important factors in CNDD.Further,we found that the gravity-dispersed tree species experienced CNDD across habitats,while for wind-dispersed species CNDD was found in gully,terrace and low-ridge habitats.Our study suggests that neglecting the habitat heterogeneity and dispersal mode can distort the signal of CNDD and community assembly in temperate forests.展开更多
Spatial patterns reveal critical features at the individual and community levels.However,how to evaluate changes in spatial characteristics remains largely unexplored.We assess spatial changes in spatial point pattern...Spatial patterns reveal critical features at the individual and community levels.However,how to evaluate changes in spatial characteristics remains largely unexplored.We assess spatial changes in spatial point patterns by augmenting current statistical functions and indices.We fitted functions to describe unmarked and marked(tree size)spatial patterns using data from a large-scale silvicultural experiment in southern Chile.Furthermore,we compute the mingling index to represent spatial tree diversity.We proffer the pair correlation function difference before and after treatment to detect changes in the unmarked-point pattern of trees and the semivariogram-ratio to evaluate changes in the marked-point pattern.Our research provides a quantitative assessment of a critical aspect of forest heterogeneity:changes in spatial unmarked and marked-point patterns.The proposed approach can be a powerful tool for quantifying the impacts of disturbances and silvicultural treatments on spatial patterns in forest ecosystems.展开更多
The spatial distribution and breeding behavior of neighboring birds(conspecific and heterospecific)may influence reproduction and the effects differ across species.In this study,we investigated intra-and inter-specifi...The spatial distribution and breeding behavior of neighboring birds(conspecific and heterospecific)may influence reproduction and the effects differ across species.In this study,we investigated intra-and inter-specific spatial distribution of breeding via artificial nestboxes and examined whether overlap with neighbors affected reproductive success of the focal breeding pair.Regarding spatial distribution,Varied Tits(Sittiparus varius)and Cinereous Tits(Parus cinereus)showed similar pattern:distances of nestboxes containing conspecific tits were greater than those with heterospecific ones.In terms of breeding behavior,reproductive success was not significantly related to the distance from their neighbor’s nest.The reproductive success of Varied Tits was significantly related to that of their neighbors,while in Cinereous Tits,no strong link was found to any recorded reproductive behavior of the neighbors.These findings explain spatial variation in nest site selection of Varied Tits and Cinereous Tits.Reproductive success rate of Varied Tits is affected by that of the neighbors while that of Cinereous Tits is not.The results highlight that there are differences in reproductive outcome among related species and offer suggestions on the use of artificial nestboxes in experiment and conservation programs.展开更多
Effects of maize (Zea mays L.) volatiles induced by larvae of the Asian corn borer, Ostriniafurnacalis (Guenee), on the orientation behaviors of Asian corn borer larvae and oviposition of the females were investig...Effects of maize (Zea mays L.) volatiles induced by larvae of the Asian corn borer, Ostriniafurnacalis (Guenee), on the orientation behaviors of Asian corn borer larvae and oviposition of the females were investigated. Nineteen volatile chemicals, with terpenes being the major components, were identified from maize plants attacked by third instar Asian corn borer larvae. Coupled gas chromatographic-electroantennographic detection (GC-EAD) analyses revealed some electroantennographic differences between female and male Asian corn borer antennae in response to larvae-induced maize volatiles; female responded to (E)-2-hexenal, nonanal, (Z)-3-hexen-l-ol and three unknown compounds while the male only responded to (E)-2-hexenal, nonanal and one unknown compound. In laboratory orientation bioassays, Asian corn borer neonate larvae were attracted to extracts collected from Asian corn borer-damaged plants as well as to synthetic farnesene, but were repelled by (Z)-3-hexen- 1 -ol. In laboratory oviposition bioassays, gravid females laid fewer eggs on plants damaged by larvae than on mechanically damaged plants or undamaged plants. Adult Asian corn borer females deposited fewer eggs on wax paper treated with (E)-2-hexenal or (Z)-3-hexen-l-ol than on wax paper treated with hexane (control). The results suggest that Asian corn borer can affect the behaviors of conspecific larvae and adults by changing host plant volatiles.展开更多
The genus Lactarius Pers.(Russulales)is a cosmopolitan group of Basidiomycota that forms ectomycorrhizal relationships primarily with both deciduous and coniferous trees.Although the genus has been well-studied in Eur...The genus Lactarius Pers.(Russulales)is a cosmopolitan group of Basidiomycota that forms ectomycorrhizal relationships primarily with both deciduous and coniferous trees.Although the genus has been well-studied in Europe and North America,only fragmentary researches have been carried out on Asian species.In particular,the distribution of Lactarius species in South Korea is poorly understood due to insufficient morphological descriptions and a lack of DNA sequence data.In addition,the misuse of European and North American names has added to confusion regarding the taxonomy of Asian Lactarius species.In this study,the diversity of Lactarius in South Korea was evaluated by employing both morphological and phylogenetic approaches.A multi-locus phylogenetic analysis of 729 Lactarius specimens collected between 1960 and 2017 was performed using the internal transcribed spacer(ITS)region,partial nuclear ribosomal large subunit(nrLSU),partial second largest subunit of RNA polymerase II(rpb2),and minichromosome maintenance complex component 7(mcm7).49 Lactarius species were identified in three Lactarius subgenera:L.subg.Russularia(17 spp.),L.subg.Lactarius(22 spp.),and L.subg.Plinthogalus(10 spp.).Among them,28 Lactarius species were identified as new to science,while just 17 were previously described Lactarius species.Four of the taxa remain un-named due to paucity of materials.A key to Korean Lactarius species,molecular phylogenies,a summary of diversity,and detailed description are provided.展开更多
An important mechanism promoting species coexistence is conspecific negative density dependence(CNDD),which inhibits conspecific neighbors by accumulating host-specific enemies near adult trees.Natural enemies may be ...An important mechanism promoting species coexistence is conspecific negative density dependence(CNDD),which inhibits conspecific neighbors by accumulating host-specific enemies near adult trees.Natural enemies may be genotype-specific and regulate offspring dynamics more strongly than non-offspring,which is often neglected due to the difficulty in ascertaining genetic relatedness.Here,we investigated whether offspring and non-offspring of a dominant species,Castanopsis eyrei,suffered from different strength of CNDD based on parentage assignment in a subtropical forest.We found decreased recruitment efficiency(proxy of survival probability)of offspring compared with non-offspring near adult trees during the seedling-sapling transition,suggesting genotype-dependent interactions drive tree demographic dynamics.Furthermore,the genetic similarity between individuals of same cohort decreased in late life history stages,indicating genetic-relatedness-dependent tree mortality throughout ontogeny.Our results demonstrate that within-species genetic relatedness significantly affects the strength of CNDD,implying genotype-specific natural enemies may contribute to population dynamics in natural forests.展开更多
文摘Artificial animals are increasingly used as conspecific stimuli in animal behavior research.However,researchers often have an incomplete understanding of how the species under study perceives conspecifics,and,hence,which features are needed for a stimulus to be perceived appropriately.To investigate the features that bearded dragons(Pogona vitticeps)attend to,we measured their lateralized eye use when assessing a successive range of stimuli.These ranged through several stages of realism in artificial conspecifics,to see how features such as color,the presence of eyes,body shape and motion influence behavior.We found differences in lateralized eye use depending on the sex of the observing bearded dragon and the artificial conspecific,as well as the artificial conspecific’s behavior.Therefore,this approach can inform the design of robotic animals that elicit biologically-meaningful responses in live animals.
基金supported by grants from the National Natural Science Foundation of China(No.31770236)the Guangxi Key Research and Development Projects(Guike AB21220057)Training Program for Academic and Technical Leaders(leading talents)in Major Disciplines of Jiangxi Province(grant to Zhiyong Zhang,20213BCJ22006)。
文摘Taxonomic uncertainties of rare species often hinder effective prioritization for conservation.One such taxonomic uncertainty is the 90-year-old enigma of Fagus chienii.F.chienii was previously only known from the type specimens collected in 1935 in Pingwu County of Sichuan Province,China,and has long been thought to be on the verge of extinction.However,morphological similarities to closely related Fagus species have led many to question the taxonomic status of F.chienii.To clarify this taxonomic uncertainty,we used the newly collected samples to reconstruct a molecular phylogeny of Chinese Fagus species against the phylogenetic backbone of the whole genus using seven nuclear genes.In addition,we examined nine morphological characters to determine whether F.chienii is morphologically distinct from its putatively closest relatives(F.hayatae,F.longipetiolata,and F.lucida).Both morphological and phylogenetic analyses indicated that F.chienii is conspecific with F.hayatae.We recommended that F.chienii should not be treated as a separate species in conservation management.However,conservation strategies such as in situ protection and ex situ germplasm preservation should be adopted to prevent the peculiar“F.chienii”population from extinction.
基金Shihong Jia was financially supported by the National Natural Science Foundation of China(Grant No.32001120)the Fundamental Research Funds for the Central Universities(Grant No.31020200QD026)+1 种基金Qiulong Yin was supported by the National Natural Science Foundation of China(Grant No.32001171)Ying Luo was supported by the Innovation Capability Support Program of Shaanxi(Grant No.2022KRM090).
文摘Conspecific negative density dependence(CNDD)is a potentially important mechanism in maintaining species diversity.While previous evidence showed habitat heterogeneity and species’dispersal modes affect the strength of CNDD at early life stages of trees(e.g.,seedlings),it remains unclear how they affect the strength of CNDD at later life stages.We examined the degree of spatial aggregation between saplings and trees for species dispersed by wind and gravity in four topographic habitats within a 25-ha temperate forest dynamic plot in the Qinling Mountains of central China.We used the replicated spatial point pattern(RSPP)analysis and bivariate paircorrelation function(PCF)to detect the spatial distribution of saplings around trees at two scales,15 and 50 m,respectively.Although the signal was not apparent across the whole study region(or 25-ha),it is distinct on isolated areas with specific characteristics,suggesting that these characteristics could be important factors in CNDD.Further,we found that the gravity-dispersed tree species experienced CNDD across habitats,while for wind-dispersed species CNDD was found in gully,terrace and low-ridge habitats.Our study suggests that neglecting the habitat heterogeneity and dispersal mode can distort the signal of CNDD and community assembly in temperate forests.
基金supported by the Chilean research grant Fondecyt No.1210147.
文摘Spatial patterns reveal critical features at the individual and community levels.However,how to evaluate changes in spatial characteristics remains largely unexplored.We assess spatial changes in spatial point patterns by augmenting current statistical functions and indices.We fitted functions to describe unmarked and marked(tree size)spatial patterns using data from a large-scale silvicultural experiment in southern Chile.Furthermore,we compute the mingling index to represent spatial tree diversity.We proffer the pair correlation function difference before and after treatment to detect changes in the unmarked-point pattern of trees and the semivariogram-ratio to evaluate changes in the marked-point pattern.Our research provides a quantitative assessment of a critical aspect of forest heterogeneity:changes in spatial unmarked and marked-point patterns.The proposed approach can be a powerful tool for quantifying the impacts of disturbances and silvicultural treatments on spatial patterns in forest ecosystems.
基金This work was supported by the National Natural Science Foundation of China(No.31872231 to DW,No.32000316 to YJ).
文摘The spatial distribution and breeding behavior of neighboring birds(conspecific and heterospecific)may influence reproduction and the effects differ across species.In this study,we investigated intra-and inter-specific spatial distribution of breeding via artificial nestboxes and examined whether overlap with neighbors affected reproductive success of the focal breeding pair.Regarding spatial distribution,Varied Tits(Sittiparus varius)and Cinereous Tits(Parus cinereus)showed similar pattern:distances of nestboxes containing conspecific tits were greater than those with heterospecific ones.In terms of breeding behavior,reproductive success was not significantly related to the distance from their neighbor’s nest.The reproductive success of Varied Tits was significantly related to that of their neighbors,while in Cinereous Tits,no strong link was found to any recorded reproductive behavior of the neighbors.These findings explain spatial variation in nest site selection of Varied Tits and Cinereous Tits.Reproductive success rate of Varied Tits is affected by that of the neighbors while that of Cinereous Tits is not.The results highlight that there are differences in reproductive outcome among related species and offer suggestions on the use of artificial nestboxes in experiment and conservation programs.
文摘Effects of maize (Zea mays L.) volatiles induced by larvae of the Asian corn borer, Ostriniafurnacalis (Guenee), on the orientation behaviors of Asian corn borer larvae and oviposition of the females were investigated. Nineteen volatile chemicals, with terpenes being the major components, were identified from maize plants attacked by third instar Asian corn borer larvae. Coupled gas chromatographic-electroantennographic detection (GC-EAD) analyses revealed some electroantennographic differences between female and male Asian corn borer antennae in response to larvae-induced maize volatiles; female responded to (E)-2-hexenal, nonanal, (Z)-3-hexen-l-ol and three unknown compounds while the male only responded to (E)-2-hexenal, nonanal and one unknown compound. In laboratory orientation bioassays, Asian corn borer neonate larvae were attracted to extracts collected from Asian corn borer-damaged plants as well as to synthetic farnesene, but were repelled by (Z)-3-hexen- 1 -ol. In laboratory oviposition bioassays, gravid females laid fewer eggs on plants damaged by larvae than on mechanically damaged plants or undamaged plants. Adult Asian corn borer females deposited fewer eggs on wax paper treated with (E)-2-hexenal or (Z)-3-hexen-l-ol than on wax paper treated with hexane (control). The results suggest that Asian corn borer can affect the behaviors of conspecific larvae and adults by changing host plant volatiles.
基金supported by the project on the survey and excavation of Korean indigenous species of the National Institute of Biological Resources(NIBR 201701104 and NIBR 201801105)under the Ministry of Environment of the Republic of Korea.
文摘The genus Lactarius Pers.(Russulales)is a cosmopolitan group of Basidiomycota that forms ectomycorrhizal relationships primarily with both deciduous and coniferous trees.Although the genus has been well-studied in Europe and North America,only fragmentary researches have been carried out on Asian species.In particular,the distribution of Lactarius species in South Korea is poorly understood due to insufficient morphological descriptions and a lack of DNA sequence data.In addition,the misuse of European and North American names has added to confusion regarding the taxonomy of Asian Lactarius species.In this study,the diversity of Lactarius in South Korea was evaluated by employing both morphological and phylogenetic approaches.A multi-locus phylogenetic analysis of 729 Lactarius specimens collected between 1960 and 2017 was performed using the internal transcribed spacer(ITS)region,partial nuclear ribosomal large subunit(nrLSU),partial second largest subunit of RNA polymerase II(rpb2),and minichromosome maintenance complex component 7(mcm7).49 Lactarius species were identified in three Lactarius subgenera:L.subg.Russularia(17 spp.),L.subg.Lactarius(22 spp.),and L.subg.Plinthogalus(10 spp.).Among them,28 Lactarius species were identified as new to science,while just 17 were previously described Lactarius species.Four of the taxa remain un-named due to paucity of materials.A key to Korean Lactarius species,molecular phylogenies,a summary of diversity,and detailed description are provided.
基金supported by the Strategic Priority Research Program of the Chinese Academy of Sciences(XDB31000000)the National Key Research and Development Program of China(2017YFA0605103)funded by the US National Science Foundation(NSF DEB-2029997)。
文摘An important mechanism promoting species coexistence is conspecific negative density dependence(CNDD),which inhibits conspecific neighbors by accumulating host-specific enemies near adult trees.Natural enemies may be genotype-specific and regulate offspring dynamics more strongly than non-offspring,which is often neglected due to the difficulty in ascertaining genetic relatedness.Here,we investigated whether offspring and non-offspring of a dominant species,Castanopsis eyrei,suffered from different strength of CNDD based on parentage assignment in a subtropical forest.We found decreased recruitment efficiency(proxy of survival probability)of offspring compared with non-offspring near adult trees during the seedling-sapling transition,suggesting genotype-dependent interactions drive tree demographic dynamics.Furthermore,the genetic similarity between individuals of same cohort decreased in late life history stages,indicating genetic-relatedness-dependent tree mortality throughout ontogeny.Our results demonstrate that within-species genetic relatedness significantly affects the strength of CNDD,implying genotype-specific natural enemies may contribute to population dynamics in natural forests.