L-DOPA is a major drug for the treatment of Parkinson’s disease,and microbial enzymatic synthesis is an essential way for its industrial production.The expression of tyrosine phenol-lyase(TPL)derived from Erwinia her...L-DOPA is a major drug for the treatment of Parkinson’s disease,and microbial enzymatic synthesis is an essential way for its industrial production.The expression of tyrosine phenol-lyase(TPL)derived from Erwinia herbicola under six diferent constitutive promoters in Escherichia coli was studied.The recombinant strain 5 can express TPL well with growth.The optimal medium composition of this strain was as follows:tryptone 18 g/L,yeast extract 28 g/L,glycerol 10 g/L.After the cell culture is completed,the collected cells were used to catalyze the production of L-DOPA,and the concentration can reach 67.9 g/L.展开更多
基金We thank the National Key Scientifc Instrument and Equipment Development Project of China(2013YQ17052504)the Program for Changjiang Scholars and Innovative Research Team in the University of Ministry of Education of China(IRT_15R55)+1 种基金the seventh group of Hundred-Talent Program of Shanxi Province(2015)The Key Project of Research and Development Plan of Shaanxi(2017ZDCXLSF-01-02-01)for fnancial support.
文摘L-DOPA is a major drug for the treatment of Parkinson’s disease,and microbial enzymatic synthesis is an essential way for its industrial production.The expression of tyrosine phenol-lyase(TPL)derived from Erwinia herbicola under six diferent constitutive promoters in Escherichia coli was studied.The recombinant strain 5 can express TPL well with growth.The optimal medium composition of this strain was as follows:tryptone 18 g/L,yeast extract 28 g/L,glycerol 10 g/L.After the cell culture is completed,the collected cells were used to catalyze the production of L-DOPA,and the concentration can reach 67.9 g/L.
