Constructing Electron Microscope Labs: Challenges and Solutions

The construction of advanced laboratories for precision instruments, such as electron microscopes, involves unique challenges that are influenced by the specific environmental conditions required for optimal functionality. These include mitigating interference from magnetic fields and vibrations, which are critical for maintaining the precision and accuracy of the instruments used. This study aims to offer enhanced project management strategies and detailed construction solutions that address the environmental and technical needs specific to electron microscopy labs, thereby facilitating effective lab operations and extending the lifecycle of high-end precision instruments. Case studies of existing laboratory constructions, onsite investigations, and comprehensive reviews of the technical and environmental requirements provide the basis for a best practice for constructing sophisticated electron microscopy labs. The approach integrates both pre-construction planning and post-construction adjustments to create optimal operational environments. The findings suggest that successful lab constructions are those that incorporate thorough onsite assessments, strategic location choices, and the use of advanced construction materials and techniques specifically designed to counteract environmental challenges like magnetic and vibration interferences. Actionable guidelines for both planning and executing the construction of electron microscope labs highlighted in this tutorial are intended as an important resource to troubleshoot or upgrade existing lab facilities and to consult in preparation of future lab construction projects.

Rapid Constructing a Genetic Linkage Map by AFLP Technique and Mapping a New Gene tms5

In this study, we reported the repaid construction of a molecular marker linkage map of rice (Oryza sativa L.). An F-2 population from the cross between Annong S-1 and Nanjing 11 was used to construct a genetic linkage map of rice. Total of 142 newly screened AFLP markers and 30 anchor markers (25 SSR markers and 5 RFLP markers) were mapped on the 12 chromosomes covering 1537.4 cM of rice genome. The average interval between these markers was 9.0 cM. The total work which usually was finished in more than one year was finished within only 3 months by one person. This is the first plant AFLP map developed in China. A new thermosensitive genic male sterile gene in rice, tms5, was Egged and mapped onto chromosome 2 during the development of the linkage map.

Acknowledging the Challenges of Constructing in Regions with Low Temperature

This research paper provides an overview of low-temperature construction processes with an emphasis on the challenges that the sector faces and the creative solutions created to deal with them. Low-temperature construction has particular challenges with regard to technology, materials, worker safety and technological development. It highlights the necessity of customized regulations and procedures aimed at improving the sustainability and efficiency of construction in regions with low temperatures.

Analysis on the Necessity and Feasibility of Implementing the Strategy of "Protecting Farmland in Flatland Areas and Constructing Mountainous Cities" in Yunnan Province

Yunnan is one of the most typical mountalnous provinces in western Chi-na. For the geographical features of mountalnous areas accounting for most parts and fIatIand areas accounting for smal parts, the contradiction between farmIand protection and construction guarantee is very prominent. In the first half of 2011, Yunnan Province estabIished creativeIy the strategy of ＆quot;protecting farmIand in fIat-Iand areas and constructing mountalnous cities＆quot; （hereinafter referred to as the strat-egy of ＆quot;constructing mountalnous cities＆quot;）, which was almed at protecting effectiveIy the Iimited and high quality arabIe Iand resources in fIatIand area and providing new construction area for new type of urbanization and industrialization, so as to achieve win-win situation between arabIe Iand protection and urbanization deveIopment. Im-pIementing this strategy in Yunnan Province is feasibIe, malnIy in the Iarge deveIop-ment potentials in the gentIe gradient between 8° to 25°. The gentIe sIope area be-tween 8° to 25° occupies 51% of the total Iand area in Yunnan Province, which is almost 140 times of the area of urban construction Iand in Yunnan in 2009. The surrounding areas of fIatIand or maln town in most counties （cities, regions） dis-tribute with different sizes of Iow hiI s and gentIe sIopes, thus mountalnous construc-tion Iand reserves are abundant, which benefits for impeI ing the strategy of ＆quot;con-structing mountalnous cities＆quot; in the Iight of Iocal conditions.

A Simplified Graphical Procedure for Constructing a 10˚or 20˚Angle

This paper presents a simplified graphical procedure for constructing, using an unmarked straightedge and a compass only, a 10˚ to 20˚ angle, which is in other words, trisecting a 30˚ or 60˚ angle. The procedure, when applied to the 30˚ and 60˚ angles that have been “proven” to be not trisectable, produced a construction having the identical angular relationship with Archimedes’ Construction, in which the required trisection angles were found to be 10.00000˚ and 20.00000˚ respectively (i.e. exactly one-third of the given angle or ∠E’MA = 1/3∠E’CG). Based on this identical angular relationship as well as the numerical results obtained, one can only conclude that the geometric requirements for arriving at an exact trisection of the 30˚ or 60˚ angle, and therefore the construction of a 10˚ or 20˚ angle, have been met, notwithstanding the theoretical proofs of Wantzel, Dudley, and others. Thus, the solution to the age-old trisection problem, with respect to these two angles, has been accomplished.

The Scale of Constructing Mountainous Cities in Yunnan Province based on “Benchmark” Farmlands

In 2011, Yunnan provincial committee and government proposed the strat- egy of constructing mountainous cities in order to protect farmlands and guarantee crop safety, which makes full use of hills and gentle-slopes and transforms land use pattern. However, social and economic development performs unevenly in Yun- nan, supplemented by varied geographical conditions, so that constructing mountain- ous cities should proceed as per local conditions. The research proposed the con- trol of constructing mountainous cities provided crop safety under the principle of farmland protection and measured the quantities of demanded construction lands ac- cording to science of population prediction and ekistics, based on which the control scales of constructing mountainous cities were determined by multiplying basic farm- land coefficient and the area of newly increased construction lands. The research is of significance for guiding the construction of mountainous cities and avoiding the occupation of construction lands on farmlands.

Study on Urban Construction Land Suitability Evaluation in Southwestern Mountainous Areas of Yunnan Province Based on the Strategy of "Protecting Farmland in Flatland Areas and Constructing Mountainous Cities" : A Case in Lianghe County

According to the particularity of towns＇ construction in the southwestern mountainous areas of Yunnan Province, this study has built constructive land suit- ability evaluation index system which is different to flat area. Then this study deter- mined single evaluation factors index and comprehensive suitability index using ＂ex- treme conditions method＂ and ＂suitability index method＂, and analyzed and assessed the constructive land suitability on each evaluation unit polygons and its suitability level by using GIS software. Taking Lianghe County of Dehong Dai-Jingpo Au- tonomous Prefecture as an example to evaluate land suitability in Lianghe County＇s main towns around the gentle slope, the results show, in the evaluation area, the area of land suitable for construction and unsuitable for construction respectively ac- count 73.58% and 26.42%. And first-class, second and third-class of land suitable for construction should be composited at the ratio of about 11：49：40. The suitability evaluation index system and method developed in this paper is applicable for evalu- ating the construction land suitability in mountainous areas of southwest Yunnan Province, and will provide theoretical and technical support for land policy measures which strengthen the protection of farmland and promote the scientific development of urbanization in Yunnan Province and even similar mountainous areas of the whole China.

Research on prevention and cure against heat-harm in constructing mines

With the mine exploitation depth getting deeper and deeper, the mine heat-harm turns into another natural disaster after the five disasters, which are the roof, the gas, the fire, the dust and the flood disaster. Compared with other researches on prevention and cure against the heat-harm, the research on prevention and cure against the heat-harm in constructing mine are less. Referring to the experience of prevention and cure against the heat-harm home and ＇abroad, analyzing the characteristic of cooling in the constructing mine, and based on the ground former freezing equipments and the modified mine temporary cooling equipments, the paper proposed four technical projects. By comparing with the four technical projects, the second project is preponderant both in technology and economy and can solve the heat-harm of the constructing mine, which is proved by the forecast and calculation. The project can be widely applied in de-temperature tasks of high temperature and heat-harm mines during the mine constructing period and in the exploiting preparing phase.

THOUGHTS ON CONSTRUCTING THE DEMONSTRATING AREAS OF THE ECOLOGICAL REBUILDING AND ECONOMIC SUSTAINABLE DEVELOPMENT IN HEXI REGION

Based on the field investigations, this paper analyzes systematically the achievements and problems of constructing the demonstrating areas of the ecological rebuilding and economic sustainable development in Hexi, Gansu Province, and then the thoughts on constructing the demonstrating areas are brought forward. It is considered that all kinds of ecological demonstrating areas should be merged in order to construct large scale characterized demonstrating areas which go beyond the district. In other words, in Hexi Region, Zhangye District should be constructed as the largest ecoagriculture demonstrating area of high technology; Wuwei District should be constructed as the largest ecological demonstrating area of agricultural comprehensive exploitation; JiuJia District, which is the shortened form of Jiuquan and Jiayuguan, should be constructed as the largest ecotourism demonstrating area; and Jinchang City should be constructed as the largest ecoindustry demonstrating area. At the same time, the constructing pattern should be selected according to the actual circumstances; science and technology should be applied to construct the demonstrating areas and accelerate the industrialization in the big market. Additionally, it is important to smooth the constructing system and implement the flexible and efficacious running mechanism, and it is suggested that the committee should be organized to administer the ecological demonstrating areas in Hexi Region.

Constructing the Virtual Production Line

In order to study the constructing technology of virtual production line, the structure model of virtual production line was presented, and the object oriented technique was used to establish its basic classes and relevant models, including solid model, behavior model and object oriented Petri net based control model, and based on this, the constructing of virtual production line was realized. The application proved that the virtual production line had many good characteristics, such as visualization, interaction, multi layer and reusability, and it's an efficient tool of analyzing and modeling for layout planning and rapidly reconfiguring of production line.

A NEW METHOD FOR CONSTRUCTING DIGITAL-STRAIN-FIELD-IMAGES FROM MOIRE PATTERNS

A new method for constructing digital-strain-field-image from a moire pattern with the help of digital image processing technique is proposed in this paper. The digital-strain-field-image expresses the strain values directly by its grey levels. The digital-strain-field-image can be obtained by making a differentiation for the digital moire pattern and doing a simple division operation. This image not only gives the visual strain field distribution, but also gives the strain values of every point. This method is simple and applicable and has satisfactory accuracy.

COIF:Focus on Posat-pandemic Period and Constructing A New Development Paradigm

On November 25,the 12th China Overseas Investment Fair(COIF)was held by the China Overseas Development Association in Beijing.Officials and representative from embassies or offices in China from 85 different countries,including the ambassadors from 31 countries,attended this year’s event.The COIF was held with the theme entitled“Concentrating on the post-pandemic era and constructing a new development paradigm”,and focused on how to build platforms and create business opportunities to lift the world economy out of recession.

Constructing Molecular Marker Linkage Maps of Chromosome 14Sh and 22Sh and QTL Mapping for Major Traits by Use of Substitution Lines of Gossypium hirsutum L.

CSB14Sh,which is isogenic for its recurrent parent TM-1 except for chromosome 14 short arm,was crossed with TM-1,and the F2 population was produced.A total of 3800 SSR primer pairs covering the whole genome were used to screen polymorphism among two parents,TM-1 and CSB14Sh,

Research on the Proper Countermeasures of Constructing the Student Centered English Classroom Atmosphere

In this paper, we conduct research on proper countermeasures of constructing the student centered English classroom atmosphere. Student-centered education essentially requires educators from the perspective of students thinking and gives full consideration to the students’ interest hobbies, need, etc., in the teaching process is given priority to with the activities of the students. Compared with traditional English teaching mode, the study of the theoretical foundation of the comprehensive practice teaching mode are rare. This paper integrates the related theories to propose the novel mode of the current English education paradigm that will be meaningful.

Constructing an assessment index system for strategic risk management in coal science and technology enterprises

Coal occupies a dominant position in China＇s national economy and is an essential energy source for future industrial development. To inform the efficient and sustainable development of the coal industry, this paper analyzes the sources of strategic risk for coal science and technology enterprises using 3 first-class indicators, 10 second-level indicators, and 37 observation points established through the existing research literature and experience. Moreover, in accordance to the obtained initial index data, the selected indicators have been tested and screened using reliability and membership degree analyses to remove redundant variables, avoid juxtaposition of risk factors at different levels, and reduce the influ- ence of some tiny risk factors for enterprise strategic risk. Then, factor analysis of external environment factor sub-scale was carried out. Factors are extracted according to a standard characteristic value greater than 1. Variables with high coefficients are classified into one factor category; and finally, 3 first-class indicators. 8 second-level indicators, and 37 observation noints are reconstructed.

Constructing Large Capacity Power Plant on Collapsible Loess Stratum with Huge Thickness

1 Preface In the northern and northwestern parts of China, quite a large portion of area, approximately 630,000 km^2, is covered by loess and loess-liked soils. The loess thickness ranges from several meters to several hundred meters along the river’s terraces to those geomorphologic plateaus. In geology, "China Loess" has become a geologic term, because the loess in China has evolved with the widest distribution and greatest thickness in the world, and is also a typical and significant deposit in Quaternary Period.

Logics of Constructing Chinese Form of Human Civilization Facing the Future:CPC and Development of Chinese Culture

As one of the oldest human civilizations,the Chinese nation once created brilliant ancient and classical culture.However,in transition to modernity,as China became a late comer of modernization,the Chinese nation has chosen the Party as the leading force in constructing its modern culture.Guided by Marxism,the Communist Party of China(CPC)has advanced

Mechanism of tolerance inducement in rat liver transplantation by constructing retroviral vector containing FasL gene

Objective To explore the further mechanism of immune tolerance induced by injecting dendritic cells transfected with Fasl gene in rat liver transplantation. Methods Orthotopic liver transplantation models in 36 Wistar rats receiving SD liver as donors were established by using" the two-cuff technique" with some modifications. The rats were randomly divided into 3 groups: CsA-treated group (n = 12) treated with cyclosporine; mdrl-treated group( n = 12) treated with dendritic cells transfected with mdrl gene and FasL group (n - 12) treated with the dendritic cells transfected with FasL gene by intraperitoneal injection. 12 normal Wistar rats served as control group. Four rats in each group were killed on the postoperative day (POD) 7 respectively. The liver and peripheral blood were obtained to determine liver function, mRNA expression of Fas, FasL, IL-2 in the grafts by semi-quantitative RT-PCR. Results It was found that the rats in the control group all died between 9-15 days after operation, and the