Objective To construct p IRES2-ZsG reen1/FⅨexpression vector,using the pcDNA/FⅨplasmid containing FⅨcDNA as template,and expressing in HEK-293cells.Methods The total ORF of FⅨgene was amlified from pcDNA/FⅨplasmi...Objective To construct p IRES2-ZsG reen1/FⅨexpression vector,using the pcDNA/FⅨplasmid containing FⅨcDNA as template,and expressing in HEK-293cells.Methods The total ORF of FⅨgene was amlified from pcDNA/FⅨplasmid,then the amplified fragment was clonded into the p IRES2-ZsG reen1 vector using展开更多
With the combination of modern quantitative analysis method and enterprise inputoutput model,in this paper we make a scientific analysis on the effect to product profit tax from the change of internal and external imp...With the combination of modern quantitative analysis method and enterprise inputoutput model,in this paper we make a scientific analysis on the effect to product profit tax from the change of internal and external important factors of enterprise,and some important results hare been given.展开更多
文摘Objective To construct p IRES2-ZsG reen1/FⅨexpression vector,using the pcDNA/FⅨplasmid containing FⅨcDNA as template,and expressing in HEK-293cells.Methods The total ORF of FⅨgene was amlified from pcDNA/FⅨplasmid,then the amplified fragment was clonded into the p IRES2-ZsG reen1 vector using
文摘With the combination of modern quantitative analysis method and enterprise inputoutput model,in this paper we make a scientific analysis on the effect to product profit tax from the change of internal and external important factors of enterprise,and some important results hare been given.
