Fosmid library construction and screening for the maize mutant gene Vestigial glume 1

The maize mutant gene Vestigial glume 1(Vg1) has been fine-mapped to a narrow region by map-based cloning and the candidate gene for Vg1 spanned 19.5 kb. Here we report Vg1 genomic fosmid library construction and screening. The fosmid library of Vg1 consisted of574,000 clones with an average insert size of 36.4 kb, representing 7.9-fold coverage of the maize genome. Fosmid stability assays indicated that clones were stable during propagation in the fosmid system. Using Vg1 candidate gene-specific primers, a positive clone was successfully identified. This discovery will pave the way for identifying the function of Vg1 in maize development.

Construction and characterization of a cDNA library from human liver tissue with chronic hepatitis B

Objective: To construct a cDNA library from human liver tissue with chronic hepatitis B and check its quality for investigating the expression level of liver tissue infected by hepatitis B virus. This will then be used to find the relevant genes and interesting proteins associated with the development of hepatitis B. Methods: The total RNA from liver tissue with chronic hepa- titis B was extracted and the mRNA was purified using TRIZOL method. Switching mechanism at 5′ end of the RNA transcript (SMART) technique and CDS III/3′ primer were used for first-strand cDNA synthesis. Long distance polymerase chain reaction (LD PCR) was then used to synthesize the double-strand cDNA that was then digested by Sfi I and fractionated by CHROMA SPIN-400 column. The longer than 0.4 kb cDNAs were collected and ligated to λTriplEx2 vector. Then λ phage packaging reaction and library amplification were performed. The qualities of both unamplified and amplified cDNA libraries were strictly checked by conventional titer determination. Fourteen plaques were randomly picked and tested using PCR with universal primers derived from the sequence flanking the vector. Results: The titers of unamplifed and amplified libraries were 1.94×106 pfu/ml and 1.49×109 pfu/ml respectively. The percentages of recombinants from both libraries were 98.15% in unamplified library and 98.76% in amplified library. The lengths of the inserts were 1.23 kb in average, 1?2 kb in 64.29%, and 0.5?1.0 kb in 35.71%. Conclusion: A high quality cDNA library from human liver tissue with chronic hepatitis B was successfully constructed.

Construction of human Fab library and screening of a single-domain antibody of amyloid-beta 42 oligomers

Screening humanized antibodies from a human Fab phage display library is an effective and quick method to obtain beta-amyloid oligomers. Thus, the present study prepared amyloid-beta 42 oli- gomers and constructed a have human Fab phage display library based on blood samples from six healthy people. After three rounds of biopanning in vitro, a human single-domain antibody that spe- cifically recognized amyloid-beta 42 oligomers was identified. Western blot and enzyme-linked im- munosorbent assay demonstrated this antibody bound specifically to human amyloid-beta 42 tetramer and nonamer, but not the monomer or high molecular weight oligomers. This study suc- cessfully constructed a human phage display library and screened a single-domain antibody that specifically recognized amyloid-beta 42 oligomers.

An Examination on the Support of Library Information to the Construction of National Research Organizations

The country’s economic development determines the political and cultural status.At the present time,China’s rapid economic development has greatly improved the country’s international prominence.Although China has high economic and political achievements,China’s cultural soft power is still in a weak position.Cultural soft power is of great significance in improving the country’s overall national strength and international competitiveness.Research organizations are an important part of the country’s soft power and are of great significance to the strengthening of national soft power.Research organizations are the“peripheral brain”in the decision-making process of the government.National government agencies need democratic science in making decisions,and the adopted policy methods must have positive and effective outcomes on the people.Therefore,the national government agencies cannot achieve this without the help of research organizations.This resulted in the development and rising construction of research organizations.With the comprehensive development and construction of research organizations,whilst making progress,has also exposed several shortcomings.The construction of China’s research organizations requires various supports;especially the intelligence agencies and access to library information,which has a unique advantage in promoting the construction of research organizations[1].This paper first introduces the definition,classification and characteristics of a research organization.It will also provide a preliminary understanding of research organizations.The paper will then highlight and analyze the problems encountered by the research organization.Finally,through the grasp of the advantages of the library and intelligence agencies,a solution is proposed.

Research on the Construction of Chinese Universities from Intelligent Library to Smart Library

The study aims to discuss the construction and development of university library,and transform the management and operation mode of university library,thereby realizing the transformation and upgrading to smart library.Taking Anhui University of Finance and Economics as the research object,first,based on the literature and data combined with questionnaire design and expert interview,data statistics was made on the development of smart library.Second,analytical hierarchy process(AHP)and big data analysis were applied to complete the construction of the smart library system.Third,based on the data analysis method,the problems in the construction of the smart library were discussed.The results showed that the Chinese smart library started late and developed rapidly after 2012;from information perception to smart service,the construction of smart library system had realized the transformation and upgrading of library construction;the key to solve the construction of smart library was to standardize library construction,realize resource co-construction and sharing,promote technological innovation,and strengthen the construction of smart librarians.To sum up,the construction of smart library is expected to make the library and librarians become the cooperative objects of teaching and scientific research in colleges and universities.

Based on the Construction of University Library and the Evaluation of Space Service Quality

In order to strengthen the construction of university library,promote the sustainable development of university library,and provide a good library space environment for university students,this paper expounds the quality evaluation of university library by establishing the evaluation index of space service quality,and puts forward the ways to improve the service quality of University Library for reference.

The plight and Countermeasures brought to the constructions of library literature by effect of college enrollment

This text introduces the concept of local literature and collection range, explains the significance of literature collection and explores building programs of local university library. Currently, university library generally put a strong emphasis on collection of academic literature, but has less attention on literature collections which reflect the situation of university location, or even worse, simply don＇ t launch business in this field. For the university library, it is a great loss of literature information resources, as well as a great regret of library work. First, what is the local literature？ It is literature data which document a region＇ s past and present issues like politics, economy, culture, education, history, geography and other aspects like important characters and events, customs, folklore etc.

Development and construction of China's higher education libraries

Libraries in China's higher education institutions have been developing in keeping pace with the flourishing development of China's higher education. This article aims to make an introduction to the construction of China's higher education libraries, especially the recent three decades' achievements since China's reform and opening-up in 1978. In this article, the authors draw a general picture of the development of libraries in China's higher education institutions, covering such eight aspects as management, types and positioning,organizational structure and personnel, expenditure and buildings, reader service, building and sharing of resources as well as automation system.

Construction and screening of suppression subtractive hybridization library of renal cell carcinoma

Objective To construct and screen the suppression subtractive hybridization (SSH) library of human renal cell carcinoma (RCC). Methods Poly A+ RNA was isolated from RCC lines 786-O(tester) and renal cell(RC) lines HK-2 ( driver), respectiely. SSH procedure was performed according to the protocol of the PCR-Select cDNA Subtraction Kit ( Clontech), and PCR products were cloned into pT-Adv vector and transformed E. coli TOP10F’. All positive clones picked out were digested and some of which were sequenced. Results The SSH library contained 362 clones with SSH cDNA fragments distributed mainly from 0.3 to 0.9 kb. Among 50 clones sequenced randomly,2 represented unknown genes and the other 48 derived from 36 known genes. Conclusion The quality of the SSH library of human RCC is reliable and is construction is the basis for further screening differentially expressed genes of RCC. 6 refs,4 figs, 1 tab.

类MyLibrary个人数字图书馆构建方案研究

分析在图书馆服务器构建类MyLibrary个人数字图书馆的必要性、意义。从资源对象及组织形式、元数据描述框架、系统主要功能模块及整体实现三个方面论述类MyLibrary个人数字图书馆构建方案。最后提出通过改造和拓展MyLibrary@LANL或Skolr的系统,并参考Refwork、360DOC等系统项目的实现方案。

Library2.0背景下体育教授个人数字图书馆的探索

在Web2.0的技术支持下,提出构建体育教授个人数字图书馆的构想,介绍了个人数字图书馆的功能和意义,重点描述了构建体育教授个人数字图书馆的具体模式,最后提出面对机遇与挑战的对策。

General review of National Digital Library development in the National Library of China

As an important part of National Digital Library(NDL),the National Digital Library Project(NDLP),which was launched in 2005,has attracted wide attention of the society in general.This paper introduces the general situation of the project from the following aspects,namely,the characteristics,objectives and contents.It also expounds on the basic ideas and the overall structure of the project and gives an introduction to the design of its basic platform,its application platform,its business management system and its standardization control system.In this paper,the digital resources construction and service delivery of the National Library of China(NLC)are introduced to cover both its present operation as well as those in conjunction with the opening of its new library building during its second-phase of library development.Finally,the most important and difficult point in the construction of the National Digital Library Project is analyzed from the following four perspectives:1)The long-term preservation of digital resources;2)the construction of knowledge organization system;3)the organic integration of the digital library and the traditional library;and 4)the risk of project management and system integration.

Investigation and Analysis on Websites of Public Libraries in Jiangxi Province

This paper analyzes the significance of the library website establishment. On this basis, the author investigates the present situation of the websites of public library at and above the count3＇ level in Jiangxi province according the investigation： we point out the problems of the public library websites in Jiangxi province, and then give some corresponding measures.

Investigation and Analysis on Websites of Public Libraries in Jiangxi Province

This paper analyzes the significance of the library website establishment. On this basis, the author investigates the present situation of the websites of public library at and above the county level in Jiangxi province according the investigation; we point out the problems of the public library websites in Jiangxi province, and then give some corresponding measures.

生成式AI视域下智慧图书馆建设的关键路径

[目的/意义]智慧图书馆已经成为当今图书馆学界和业界最热门的主题之一。智慧图书馆建设的理论研究与实践工作已经取得了初步成效,生成式AI也在蓬勃发展的同时参与到各行各业的建设中。因此有必要立足当下发展,放眼远景目标,确定生成式AI参与智慧图书馆建设的策略。[方法/过程]本文从智慧图书馆空间升级、服务打造、业务重组、人才培养、体系建设5个方面阐明了生成式AI视域下智慧图书馆建设的关键路径。[结果/结论]关键路径研究将为生成式AI引入智慧图书馆下一阶段的建设工作提供借鉴与依据。

A brief introduction to the development of the public librarianship in China

This paper briefly introduces the development status,background and prospect of the public librarianship in China.It contains a brief analysis of the basic situation of the public library in China at present according to the data announced by the government,and gives an explanation about China's public library orientation,working condition,service characteristics and existing problems.In addition,the author provides a detailed description of the library's present working emphasis in order to present readers with an overall view of the basic condition of China's public library development at present time.

Development in theoretical study and practice of library legislation in China

One of the most outstanding characteristics of library legislation in China is that theoretical research and legislative practice are mutually reinforcing, making important issues and basic rules and regulations closely associated with legislation and the research focus. A national library law is currently being enacted while several local library legislations have already seen fruitful results. In the enactment of 'Regulations for the Protection of Information Network Dissemination Rights', the library professional participated for the first time in enacting a national copyright law, which led to unprecedented flourishing of library activities and copyright studies. The promulgation of another legal framework,'Government Information Disclosure Regulations' further advanced the research on related issues and pushed forward government information services in public libraries in the same way. A new landmark for library legislation in recent years is the promulgation of'Guidelines for Land Utilization for Public Library Construction' and 'Public Library Construction Standards', while the framing of 'Rules of Professional Ethics for Librarians in China(On trial)' and the 'Library Service Manifesto' give indication that a framework of self-disciplinary measures of library professionals is established.

数智背景下图书馆红色资源建设与开发利用研究

文章从数智技术赋能下的红色资源集成与互动体验、空间创设与体验设计以及数字化传播与教育启蒙三个方面对国内外图书馆典型案例进行分析,总结了数智技术在图书馆红色资源建设和利用实践上的理论反思与实践意义,在此基础上提出我国图书馆红色资源建设和利用的策略,旨在为图书馆加强红色资源建设和利用提供新的视角和方向。

公安实践教学中数字化标本库的构建与应用

为推进信息化教学的发展,提出在公安实践教学中建设数字化标本库的构想。以虚拟仿真教学中的毒品数字化标本库构建与教学组织应用为例,探讨公安数字化标本库建设可持续发展的路径和内涵,提出适合公安特色数字化标本库内容和标准化建设的建议,采用资源利用、整合、共建等高效节约办法,注重数字化样本库和数据库同步开发使用的发展趋势。数字化标本库具有线上自学、线上线下教学、终身学习等优势,可启发公安教育智慧教学工作高质量发展。

大语言模型时代智慧图书馆建设思考:“三位一体”结构演化

大语言模型的诞生给图书馆智慧化升级提供了驱动力。文章采用复杂性系统“三位一体”(即信息化、模型化、行动化)的框架分析智慧图书馆的演进过程,探讨每个阶段的特点和图书馆系统的架构变化,识别智慧图书馆当前所处的发展阶段,并探讨它向模型系统的演进路径以及实践策略。研究认为,通过系统集成、业务流程优化、数据安全保障以及减少幻觉现象的策略,可以有效应对LLM驱动下智慧图书馆升级过程中的挑战。