It needs the foundation of system and the guarantee of organizational system for anti-corruption,but it is more necessary to build and form an effective anti-corruption mechanism,so that the anti-corruption can be rea...It needs the foundation of system and the guarantee of organizational system for anti-corruption,but it is more necessary to build and form an effective anti-corruption mechanism,so that the anti-corruption can be really put into practice. Anti-corruption mechanism refers to a organic operation system of the interaction,interconnection and constraint between the constituent elements( parts) and elements of national anti-corruption,and as a system,anti-corruption mechanism should have the characteristics of system aticness,comprehensiveness,transparency,legalization,public participation,scientific dynam ic,and internationalism. The construction of deepening anti-corruption mechanism is the need for reconstructing the ruling legitimacy of the party and the governm ent. Adhering to the principle of treating both root causes and symptoms is necessary in the construction of anti-corruption m echanism,com bating and punishing corruption is an important part of anti-corruption,and the prevention and control of corruption is the basic project of anti-corruption. Therefore,the construction of prevention and control mechanism in the anti-corruption mechanism has a more far-reaching significance.展开更多
Each possible pair of residues inβ-1,4 glucanase for disulfide formation was assessed using online websites,and four pairs L28C-S256C,Q41C-P278C,S122C-N163C and A184C-A215C were selected.Accordingly,four recombinant ...Each possible pair of residues inβ-1,4 glucanase for disulfide formation was assessed using online websites,and four pairs L28C-S256C,Q41C-P278C,S122C-N163C and A184C-A215C were selected.Accordingly,four recombinant plasmids pET28a(+)EccslH28,pET28a(+)EccslH41,pET28a(+)EccslH122 and pET28a(+)EccslH184 were prepared and transformed into E.coli to express the recombinant enzymes.Then analysis on enzymatic properties showed that T50 of the recombinant enzymes was increased from 10 min for EccslHt2 to 90 min for EccslH28 and 40 min for EccslH41 at 70℃,while their optimum pH value and pH stability were not affected,which proved that the introduction of disulfide bond improved the thermal stability ofβ-1,4 glucanase.展开更多
The complexity of the IH-635 Managed Lanes Project, located in Dallas County, Texas, posed several technical and constructive challenges, leading to the adoption of solutions different from the traditional. Two altern...The complexity of the IH-635 Managed Lanes Project, located in Dallas County, Texas, posed several technical and constructive challenges, leading to the adoption of solutions different from the traditional. Two alternative solutions for the pier cap on one of the bridge crossings over IH-35E in the IH-635 project were analyzed in this case study, a cast-in-place post-tensioned concrete cap and an innovative prefabricated steel-concrete com- posite cap. The approach was to use an estimation of direct costs for material and labor and consideration of con- struction time schedules. A supplementary numerical modeling confirmed that both alternatives behave elasti- cally under imposed loads. The direct cost of material and labor for the two alternatives were close. However, the composite alternative required 13 days less construction time, resulting in substantial cost savings from traffic closing in the very busy traffic corridor. Traffic closing costs were substantially higher than the direct costs, especially for the post-tensioned cap. The quantification of the benefits allows more confidence in the utilization of the composites caps, leading to faster completion of bridge projects and substantial economic savings.展开更多
文摘It needs the foundation of system and the guarantee of organizational system for anti-corruption,but it is more necessary to build and form an effective anti-corruption mechanism,so that the anti-corruption can be really put into practice. Anti-corruption mechanism refers to a organic operation system of the interaction,interconnection and constraint between the constituent elements( parts) and elements of national anti-corruption,and as a system,anti-corruption mechanism should have the characteristics of system aticness,comprehensiveness,transparency,legalization,public participation,scientific dynam ic,and internationalism. The construction of deepening anti-corruption mechanism is the need for reconstructing the ruling legitimacy of the party and the governm ent. Adhering to the principle of treating both root causes and symptoms is necessary in the construction of anti-corruption m echanism,com bating and punishing corruption is an important part of anti-corruption,and the prevention and control of corruption is the basic project of anti-corruption. Therefore,the construction of prevention and control mechanism in the anti-corruption mechanism has a more far-reaching significance.
基金Supported by the National Key Research and Development Plan of China(2019YFC1905902,2019YFC1905900)Key Research and Development Plan in Shandong Province(2020CXGC010603,2021ZDSYS10,2022CXGC020206)+2 种基金"Open Competition Mechanism"Project of Qilu University of Technology(Shandong Academy of Sciences)(2022JBZ01-06)Taishan Industry Leading Talent Program(tscy20180103)National Natural Science Foundation of China(31801527)。
文摘Each possible pair of residues inβ-1,4 glucanase for disulfide formation was assessed using online websites,and four pairs L28C-S256C,Q41C-P278C,S122C-N163C and A184C-A215C were selected.Accordingly,four recombinant plasmids pET28a(+)EccslH28,pET28a(+)EccslH41,pET28a(+)EccslH122 and pET28a(+)EccslH184 were prepared and transformed into E.coli to express the recombinant enzymes.Then analysis on enzymatic properties showed that T50 of the recombinant enzymes was increased from 10 min for EccslHt2 to 90 min for EccslH28 and 40 min for EccslH41 at 70℃,while their optimum pH value and pH stability were not affected,which proved that the introduction of disulfide bond improved the thermal stability ofβ-1,4 glucanase.
基金herein for allowing the use of various data from the LBJ Project in the development of this paper
文摘The complexity of the IH-635 Managed Lanes Project, located in Dallas County, Texas, posed several technical and constructive challenges, leading to the adoption of solutions different from the traditional. Two alternative solutions for the pier cap on one of the bridge crossings over IH-35E in the IH-635 project were analyzed in this case study, a cast-in-place post-tensioned concrete cap and an innovative prefabricated steel-concrete com- posite cap. The approach was to use an estimation of direct costs for material and labor and consideration of con- struction time schedules. A supplementary numerical modeling confirmed that both alternatives behave elasti- cally under imposed loads. The direct cost of material and labor for the two alternatives were close. However, the composite alternative required 13 days less construction time, resulting in substantial cost savings from traffic closing in the very busy traffic corridor. Traffic closing costs were substantially higher than the direct costs, especially for the post-tensioned cap. The quantification of the benefits allows more confidence in the utilization of the composites caps, leading to faster completion of bridge projects and substantial economic savings.
