The plasmid pGSA1285 was first modified by substituting its GUS sequence with the Chalcone synthase intron fragment from vector pFGC5941 to get the plant silencing expression vector that contained Kanamycin resistance...The plasmid pGSA1285 was first modified by substituting its GUS sequence with the Chalcone synthase intron fragment from vector pFGC5941 to get the plant silencing expression vector that contained Kanamycin resistance site and was named as pGSA2285. Using PCR-based amplification, two different restriction sites at both ends of tobacco Bax inhibitor-1 （NtBI-I） gene were created, respectively, which made the construction of ihpRNA gene silencing vector more efficiently. Then, NtBI-1 genes were inserted into Multiple Cloning Site （MCS） of pGSA2285 respectively to form Bax inhibitor-1 ihpRNA gene silencing vector, named as pGSA4285, containing sense and anti-sense BI-1 sequence which was spliced by chalcone synthase intron. Combined PCR identification and enzyme restriction analyses, the results showed that Bax inhibitor-1 ihpRNA gene silencing vector had been constructed and transferred into Agrobacterium tumefaciens EHA105 successfully, which laid a foundation for the further study on the function of BI-1 in plant PCD regulation.展开更多
Among all of achivements,17 of them won the prize of technical progress awarded by the State and CNPC.About 90%of the achi vements has been ap-plied in the production of oilfields.For instance,a vast amount of electri...Among all of achivements,17 of them won the prize of technical progress awarded by the State and CNPC.About 90%of the achi vements has been ap-plied in the production of oilfields.For instance,a vast amount of electricity and heat energy was econ-omized by raising 5%--10%of the average efficien-cy for the high efficiency furnace and pump.More than 10 excellent softwares got nationwide applica-tion in the oilfields for improving the level of plan and design.展开更多
基金Supported by National Natural Science Foundation of China (30500352)China Agricultural University URP Project
文摘The plasmid pGSA1285 was first modified by substituting its GUS sequence with the Chalcone synthase intron fragment from vector pFGC5941 to get the plant silencing expression vector that contained Kanamycin resistance site and was named as pGSA2285. Using PCR-based amplification, two different restriction sites at both ends of tobacco Bax inhibitor-1 （NtBI-I） gene were created, respectively, which made the construction of ihpRNA gene silencing vector more efficiently. Then, NtBI-1 genes were inserted into Multiple Cloning Site （MCS） of pGSA2285 respectively to form Bax inhibitor-1 ihpRNA gene silencing vector, named as pGSA4285, containing sense and anti-sense BI-1 sequence which was spliced by chalcone synthase intron. Combined PCR identification and enzyme restriction analyses, the results showed that Bax inhibitor-1 ihpRNA gene silencing vector had been constructed and transferred into Agrobacterium tumefaciens EHA105 successfully, which laid a foundation for the further study on the function of BI-1 in plant PCD regulation.
文摘Among all of achivements,17 of them won the prize of technical progress awarded by the State and CNPC.About 90%of the achi vements has been ap-plied in the production of oilfields.For instance,a vast amount of electricity and heat energy was econ-omized by raising 5%--10%of the average efficien-cy for the high efficiency furnace and pump.More than 10 excellent softwares got nationwide applica-tion in the oilfields for improving the level of plan and design.
