CONDITIONED MEDIUM OF HUMAN NASOPHARYNGEAL CARCINOMA EPITHELIOID CELL LINE CNE_(1) CONTAINED THE ACTIVITIES OF TRANSFORMED GROWTH-INHIBITING FACTORS

The hormone defined serum free conditioned medium (SFCM) of human nasopharyngeal carcinoma epithelioid cell line (CNE1) was assayed by both the 3H-thymidine incorporation test and the soft agar test. It was found that the SFCM stimulated the growth of long-term serum-free cultured CNE4 cells in ac-cordence with the fact that the growth rate of long-term serum-free cultured CNE1 cells was directly proportional to the plating density. Alternatively 5% SFCM inhibited the growth of short-term serum-free cultured CNE4 cells by 51% in which the indicator cell remained the responsiveness state of growing in the serum-supplemented medium to the effector of interest. Furthermore, SFCM resulted in the inhibition of anchorage-independent growth of CNE4 cells and A431 cells. Also in soft agar test. SFCM reduced the colony formation of NRK(?),9F cells in the presence of EGF or EGF plus TGF-β. These finding suggested that CNE4 secreted autocrine growth stimulating factor(s) and growth inhibiting factor(s) in the serum-free medium, the latter strongly reverse malignant phenotypes of CNE4 and A431 cells in serum-supplemented surrounding.

航空运输集装托盘结构损伤模拟实验研究

为提高航空运输集装托盘使用的安全性与经济性,给航空运输集装托盘结构健康监测技术研究提供一种损伤模拟方法,使用幅值和相位综合损伤因子、幅值无关损伤因子和相位无关损伤因子3种损伤因子计算方法,分别计算不同激励信号中心频率下,粘贴吸波胶、粘贴钢球、结构状态微变和噪声,以及不同面积结构真实损伤的损伤因子。实验结果表明:可以使用粘贴钢球的方式模拟航空运输集装托盘发生损伤,Lamb波的激励信号中心频率宜选择在100~120 k Hz。