The objective of the present study was to investigate the effect of outdoor grazing on the expression of genes involved in muscle growth and the nutrient contents of skeletal muscle in steers. Ten Japanese Black steer...The objective of the present study was to investigate the effect of outdoor grazing on the expression of genes involved in muscle growth and the nutrient contents of skeletal muscle in steers. Ten Japanese Black steers were divided into two groups: grazing (GR) and concentrate (CT) groups. Crude protein, extractable lipid, moisture, fatty acid, cooking loss and Warner Bratzler shear force in muscle tissue were analyzed. The gene expression of myosin heavy chain (MyHC) isoform (2a, 2x and slow), myostatin, follistatin, peroxisome proliferator-activated receptor γ2 (PPARγ2), CCAAT/enhancer binding protein α (C/EBPα), heat shock protein (HSP) 27 and HSP40 in skeletal muscles was evaluated at the end of fattening. Decreases in MyHC-2a and MyHC-2x (fast-twitch fiber type) expression in the longissimus lumborum (LL) muscle were detected in the GR group compared with the CT group;in contrast, an increase in MyHC-slow (slow-twitch fiber type) expression was shown in the GR group. These results suggest that grazing initiated muscle fiber type conversion to slow-twitch from fast-twitch. A decrease in extractable lipid content was observed in the GR group in the LL and semitendinosus (ST) muscles. Crude protein content in the LL muscle in the GR group was higher than in the CT group. MyHC expression in LL muscle in the GR group was greater than in the CT group. A decrease in myostatin and PPARγ2 gene expression was detected in the GR group compared with the CT group in both muscles. Expression of C/EBPα in LL muscle in the GR group was lower than in the CT group. These results suggest that grazing steers at the end of fattening may lead to an increase in protein content and a decrease in fat accumulation in LL and/or ST muscles by regulation of myostatin, MyHC, PPARγ2 and C/EBPα gene expression.展开更多
【目的】糖外排转运蛋白(sugars will eventually be exported transporters,SWEETs)在植物生长发育过程中发挥重要作用,解析SWEETs基因在枸杞果实发育过程中对糖积累作用,为进一步揭示SWEETs基因在枸杞果实发育过程中的作用提供参考。...【目的】糖外排转运蛋白(sugars will eventually be exported transporters,SWEETs)在植物生长发育过程中发挥重要作用,解析SWEETs基因在枸杞果实发育过程中对糖积累作用,为进一步揭示SWEETs基因在枸杞果实发育过程中的作用提供参考。【方法】用生物信息学方法对枸杞SWEET基因(LbaSWEETs)进行全基因组鉴定,并用已发表的转录数据分析LbaSWEETs在果实发育时期的基因表达情况。【结果】枸杞SWEET基因家族共有37个成员,随机分布于10条染色体上,分别编码152~621个氨基酸,蛋白质分子质量为16.87~69.97 kD,等电点为4.96~9.86。亚细胞定位预测位于叶绿体或质膜,大多数含有7个跨膜螺旋。系统进化分析发现,37个LbaSWEETs蛋白可分为4个亚群,每个亚群的基因结构和保守基序组成相似。启动子元件分析表明:Lba-SWEETs基因启动子富含大量激素响应、逆境胁迫和生长发育响应元件。转录组数据和qRT-PCR分析表明:LbaSWEET9和LbaSWEET29基因表达量随果实成熟呈现显著增加。相关性分析结果表明,LbaSWEET9和LbaSWEET29基因表达量与果糖含量呈显著正相关。【结论】LbaSWEET9和LbaSWEET29基因是果糖积累的关键基因。展开更多
Sophora moorcroftiana is an endemic, droughtresistant shrub that grows in Tibet and has some degree of resistance to salt, cold, heat, and drought. In the present study, four dehydration responsive element-binding(D...Sophora moorcroftiana is an endemic, droughtresistant shrub that grows in Tibet and has some degree of resistance to salt, cold, heat, and drought. In the present study, four dehydration responsive element-binding(DREB) genes(Sm DREB1, Sm DREB2, Sm DREB and Sm DREB1) were isolated from S. moorcroftiana for the first time and their expression and proline content under abiotic stress were analyzed. Proline accumulated in seedlings under drought, salt, cold, and heat stress treatments. The four genes were variously expressed in response to the four abiotic stresses. Sm DREB1 was induced by drought, cold, and heat stresses; Sm DREB2 and Sm DREB4 were both induced by salt, cold, and heat stresses, whereas Sm DREB3 was induced by drought and heat stresses. Thus, these four genes may participate in conferring tolerance to these four abiotic stresses and are candidate genes for genetic engineering in the future.展开更多
In the flavonoid biosynthesis pathway, Chalcone synthase (CHS) is involved in the formation of the pigment and has been shown to be a rate-limiting enzyme for the synthesis of flavonoids. In this study, a PCR approach...In the flavonoid biosynthesis pathway, Chalcone synthase (CHS) is involved in the formation of the pigment and has been shown to be a rate-limiting enzyme for the synthesis of flavonoids. In this study, a PCR approach was used to clone a Chalcone synthases cDNA from flower of sweet osmanthus “Chenghong Dangui” and it was designated as OfCHS (O. fragrans, CHS). The cDNA was 1383 bp long and a coding sequence (CDS) of 1173 bp encoding a polypeptide of 391 amino acids with an estimated molecular mass of 39.9 kDa. The theoretical isoelectric point was 6.23. Phylogenetic analysis demonstrated that OfCHS clustered with Olea europaea, Solenostemon scutellarioides, Perilla frutescens, Antirrhinum majus and Digitalis lanata. We also detected the expression of OfCHS in different tissues in “Dangui” and in two cultivars with varied coloration, “Zi Yingui” and “Chenghong Dangui” at different floral stages using quantitative real-time PCR. We observed that OfCHS transcript was higher in leaves than in petals in “Dangui”. The transcripts of OfCHS in “Zi Yingui” petals were higher than those in “Dangui” at three stages especially at xianyan stage and there was no significant difference between the two cultivars in the full flowering stage. “Chenghong Dangui” has a relatively high anthocyanin content compared to “Zi Yingui”. The relative amount of anthocyanin of “Chenghong Dangui” initially increases, and then decreases during the bloom period. However, the expression of CHS is the highest at the initial flowering stage. These data suggest that the OfCHS does not play a key role in the accumulation of total flavonoid in this cultivar. These data could contribute to explain the different accumulation of flavonoids in petals of the two cultivars.展开更多
Manual fruit thinning(MFT)in fruit trees has been previously shown to increase fruit size and enhance fruit quality,but the effect of MFT on Ponkan(Citrus reticulata Blanco)and the underlying mechanisms remain poorly ...Manual fruit thinning(MFT)in fruit trees has been previously shown to increase fruit size and enhance fruit quality,but the effect of MFT on Ponkan(Citrus reticulata Blanco)and the underlying mechanisms remain poorly understood.In this study,efforts were made to elucidate how MFT influences the fruit quality of Ponkan.The results showed that MFT substantially increased fruit size and elevated fruit total soluble solids in comparison with the fruit from the unthinned trees(used as control).Expression analyses demonstrated that m RNA abundance of three important sugar transporter genes,including CrSUT1,CrSTP1 and CrTMT1,was evidently elevated in the flesh of thinned fruit when compared with those of the control.In addition,MFT prominently up-regulated the transcript levels of various auxin and gibberellin(GA)biosynthesis and signaling genes,including CrYUC6,CrAUX/IAA,CrGA20ox1 and CrGA3ox1.Concurrently,the contents of endogenous IAA and GA3,measured at 90 d after fruit thinning,were notably elevated in the fruit from trees with the thinning treatment relative to the control,although no difference was detected in the two groups before the thinning manipulation.Taken together,these results indicate that manual fruit thinning could greatly improve fruit quality,which may be attributed to promoting fruit expansion due to the increased auxin levels and expediting sugar accumulation through the up-regulation of sugar transporter genes.展开更多
Brassica napus is an important cash crop broadly grown for the vegetable and oil values.Yellow-seeded B.napus is preferred by breeders due to its improved oil and protein quality,less pigments and lignin compared with...Brassica napus is an important cash crop broadly grown for the vegetable and oil values.Yellow-seeded B.napus is preferred by breeders due to its improved oil and protein quality,less pigments and lignin compared with the blackseeded counterpart.This study compared the differences in flavonoid and fatty acid contents between yellow rapeseed from the progenies of B.napus-Sinapis alba somatic hybrids and the black-seeded counterpart using RNA-seq analysis.Through HPLC-PDA-ESI(-)/MSanalysis,it was found that phenylpropanoids and flavonoids(i.e.,isorhamnetin,epicatechin,kaempferol,and other derivatives)in yellow seed were significantly lower than those in black seed.The fatty acid(FA)content in yellow rapeseed was higher than that in black rapeseed due to the variation of C16:0,C18:0,C18:1,C18:2,and C18:3 contents.RNA-seq analysis of seeds at four and five weeks after flowering(WAF)indicated that differentially expressed genes(DEGs)between black and yellow rapeseeds were enriched in flavonoid and FA biosynthesis,including BnTT3,BnTT4,BnTT18,and BnFAD2.Also,genes related to FA biosynthesis,desaturation and elongation(FAD3,LEC1,FUS3,and LPAT2)in yellow seed were up-regulated compared to those in black seed,while genes involved in beta-oxidation cycle(AIM1 and KAT2)of yellow seed were down-regulated compared to those in black seed.The DEGs related to the variation of flavonoids,phenylpropanoids,and FAs would help improve the knowledge of yellow seed character in B.napus and promote rapeseed improvement.展开更多
Improving grain amino acid content of rice(Oryza sativa L.)is essential for the health of consumers.This study was conducted to identify the physiological processes that contribute to the higher grain amino acid conte...Improving grain amino acid content of rice(Oryza sativa L.)is essential for the health of consumers.This study was conducted to identify the physiological processes that contribute to the higher grain amino acid content in hybrid rice cultivar Lingliangyou 268 compared to Luliangyou 996.The results showed that total amino acid content in grains was 9%higher in Lingliangyou 268 than in Luliangyou 996.There was no significant difference in grain nitrogen(N)content between Lingliangyou 268 and Luliangyou 996,while ratio of amino acid to N was 6%higher in Lingliangyou 268 compared to Luliangyou 996.A total of 16 differentially expressed proteins related to amino acid metabolism(e.g.,erythronate-4-phosphate dehydrogenase domain containing protein)were identified in grains between Lingliangyou 268 and Luliangyou 996.The identified proteins were involved in 10 molecular functions.Six of the 10 defined functions were related to binding(heterocyclic compound binding,nucleoside phosphate binding,nucleotide binding,organic cyclic compound binding,protein binding,and small molecule binding)and the other 4 defined functions were catalytic activity,enzyme regulator activity,hydrolase activity,and transferase activity.These results indicate that the higher grain amino acid content in Lingliangyou 268 compared to Luliangyou 996 is attributed to increased efficiency of converting N to amino acid that results from altered expression of proteins related to amino acid metabolism.展开更多
Phosphoenolpyruvate carboxylase (PEPC) is widely distributed in plants and bacteria, and catalyzes the carboxylation of phosphoenolpyruvate to form oxaloacetate and inorganic phosphate. To investigate the molecular ...Phosphoenolpyruvate carboxylase (PEPC) is widely distributed in plants and bacteria, and catalyzes the carboxylation of phosphoenolpyruvate to form oxaloacetate and inorganic phosphate. To investigate the molecular mechanisms of the regulation and control of peanut oil, with the degenerated primers and RACE-PCR approach, five PEPC genes were cloned from peanut, and designated as AhPEPC1, AhPEPC2, AhPEPC3, AhPEPC4, and AhPEPC5, respectively. The structure and phylogenetic analysis of PEPC protein indicated that AhPEPC1-4 genes encoded a typical plant-type PEPC-enzyme, and AhPEPC5 a bacterial-type. By real-time quantitative RT-PCR approach the expression pattern of each gene was detected in various tissues of normal and high oil-content peanut varieties. It was found that there was a lower expression level of AhPEPCs genes except for the AhPEPC2 in high-oil peanut than normal-oil peanut line. The results provide some fundamental information for the further investigation of plant PEPC proteins and their role in regulation of oil-content in peanut seeds.展开更多
文摘The objective of the present study was to investigate the effect of outdoor grazing on the expression of genes involved in muscle growth and the nutrient contents of skeletal muscle in steers. Ten Japanese Black steers were divided into two groups: grazing (GR) and concentrate (CT) groups. Crude protein, extractable lipid, moisture, fatty acid, cooking loss and Warner Bratzler shear force in muscle tissue were analyzed. The gene expression of myosin heavy chain (MyHC) isoform (2a, 2x and slow), myostatin, follistatin, peroxisome proliferator-activated receptor γ2 (PPARγ2), CCAAT/enhancer binding protein α (C/EBPα), heat shock protein (HSP) 27 and HSP40 in skeletal muscles was evaluated at the end of fattening. Decreases in MyHC-2a and MyHC-2x (fast-twitch fiber type) expression in the longissimus lumborum (LL) muscle were detected in the GR group compared with the CT group;in contrast, an increase in MyHC-slow (slow-twitch fiber type) expression was shown in the GR group. These results suggest that grazing initiated muscle fiber type conversion to slow-twitch from fast-twitch. A decrease in extractable lipid content was observed in the GR group in the LL and semitendinosus (ST) muscles. Crude protein content in the LL muscle in the GR group was higher than in the CT group. MyHC expression in LL muscle in the GR group was greater than in the CT group. A decrease in myostatin and PPARγ2 gene expression was detected in the GR group compared with the CT group in both muscles. Expression of C/EBPα in LL muscle in the GR group was lower than in the CT group. These results suggest that grazing steers at the end of fattening may lead to an increase in protein content and a decrease in fat accumulation in LL and/or ST muscles by regulation of myostatin, MyHC, PPARγ2 and C/EBPα gene expression.
文摘【目的】糖外排转运蛋白(sugars will eventually be exported transporters,SWEETs)在植物生长发育过程中发挥重要作用,解析SWEETs基因在枸杞果实发育过程中对糖积累作用,为进一步揭示SWEETs基因在枸杞果实发育过程中的作用提供参考。【方法】用生物信息学方法对枸杞SWEET基因(LbaSWEETs)进行全基因组鉴定,并用已发表的转录数据分析LbaSWEETs在果实发育时期的基因表达情况。【结果】枸杞SWEET基因家族共有37个成员,随机分布于10条染色体上,分别编码152~621个氨基酸,蛋白质分子质量为16.87~69.97 kD,等电点为4.96~9.86。亚细胞定位预测位于叶绿体或质膜,大多数含有7个跨膜螺旋。系统进化分析发现,37个LbaSWEETs蛋白可分为4个亚群,每个亚群的基因结构和保守基序组成相似。启动子元件分析表明:Lba-SWEETs基因启动子富含大量激素响应、逆境胁迫和生长发育响应元件。转录组数据和qRT-PCR分析表明:LbaSWEET9和LbaSWEET29基因表达量随果实成熟呈现显著增加。相关性分析结果表明,LbaSWEET9和LbaSWEET29基因表达量与果糖含量呈显著正相关。【结论】LbaSWEET9和LbaSWEET29基因是果糖积累的关键基因。
基金supported by the National Natural Science Foundation of China(31260189)Collaborative Innovation Center Construction of Research and Development on Tibetan Characteristic Agricultural and Animal Husbandry Resources on plateau ecologyAnimal Husbandry Resources and Promotion Plan of Plateau Basic Ecological Academic Team Ability,Agricultural and Animal Husbandry College,Tibet University
文摘Sophora moorcroftiana is an endemic, droughtresistant shrub that grows in Tibet and has some degree of resistance to salt, cold, heat, and drought. In the present study, four dehydration responsive element-binding(DREB) genes(Sm DREB1, Sm DREB2, Sm DREB and Sm DREB1) were isolated from S. moorcroftiana for the first time and their expression and proline content under abiotic stress were analyzed. Proline accumulated in seedlings under drought, salt, cold, and heat stress treatments. The four genes were variously expressed in response to the four abiotic stresses. Sm DREB1 was induced by drought, cold, and heat stresses; Sm DREB2 and Sm DREB4 were both induced by salt, cold, and heat stresses, whereas Sm DREB3 was induced by drought and heat stresses. Thus, these four genes may participate in conferring tolerance to these four abiotic stresses and are candidate genes for genetic engineering in the future.
文摘In the flavonoid biosynthesis pathway, Chalcone synthase (CHS) is involved in the formation of the pigment and has been shown to be a rate-limiting enzyme for the synthesis of flavonoids. In this study, a PCR approach was used to clone a Chalcone synthases cDNA from flower of sweet osmanthus “Chenghong Dangui” and it was designated as OfCHS (O. fragrans, CHS). The cDNA was 1383 bp long and a coding sequence (CDS) of 1173 bp encoding a polypeptide of 391 amino acids with an estimated molecular mass of 39.9 kDa. The theoretical isoelectric point was 6.23. Phylogenetic analysis demonstrated that OfCHS clustered with Olea europaea, Solenostemon scutellarioides, Perilla frutescens, Antirrhinum majus and Digitalis lanata. We also detected the expression of OfCHS in different tissues in “Dangui” and in two cultivars with varied coloration, “Zi Yingui” and “Chenghong Dangui” at different floral stages using quantitative real-time PCR. We observed that OfCHS transcript was higher in leaves than in petals in “Dangui”. The transcripts of OfCHS in “Zi Yingui” petals were higher than those in “Dangui” at three stages especially at xianyan stage and there was no significant difference between the two cultivars in the full flowering stage. “Chenghong Dangui” has a relatively high anthocyanin content compared to “Zi Yingui”. The relative amount of anthocyanin of “Chenghong Dangui” initially increases, and then decreases during the bloom period. However, the expression of CHS is the highest at the initial flowering stage. These data suggest that the OfCHS does not play a key role in the accumulation of total flavonoid in this cultivar. These data could contribute to explain the different accumulation of flavonoids in petals of the two cultivars.
基金supported by the Hubei Provincial Agriculture Research System,Chinathe Special Program for Technology Innovation of Hubei Province,China(2020BBA036)the Hubei Provincial Agricultural Science and Technology Innovation,China。
文摘Manual fruit thinning(MFT)in fruit trees has been previously shown to increase fruit size and enhance fruit quality,but the effect of MFT on Ponkan(Citrus reticulata Blanco)and the underlying mechanisms remain poorly understood.In this study,efforts were made to elucidate how MFT influences the fruit quality of Ponkan.The results showed that MFT substantially increased fruit size and elevated fruit total soluble solids in comparison with the fruit from the unthinned trees(used as control).Expression analyses demonstrated that m RNA abundance of three important sugar transporter genes,including CrSUT1,CrSTP1 and CrTMT1,was evidently elevated in the flesh of thinned fruit when compared with those of the control.In addition,MFT prominently up-regulated the transcript levels of various auxin and gibberellin(GA)biosynthesis and signaling genes,including CrYUC6,CrAUX/IAA,CrGA20ox1 and CrGA3ox1.Concurrently,the contents of endogenous IAA and GA3,measured at 90 d after fruit thinning,were notably elevated in the fruit from trees with the thinning treatment relative to the control,although no difference was detected in the two groups before the thinning manipulation.Taken together,these results indicate that manual fruit thinning could greatly improve fruit quality,which may be attributed to promoting fruit expansion due to the increased auxin levels and expediting sugar accumulation through the up-regulation of sugar transporter genes.
基金funded by the National Natural Science Foundation of China(U20A2028 and 31972963)the Open Funds of the Key Laboratory of Plant Functional Genomics of the Ministry of Education,China(ML201804)+2 种基金the Project of Special Funding for Crop Science Discipline Development,China(yzuxk202006)the Priority Academic Program Development of Jiangsu Higher Education Institutions,Chinathe Yangzhou University for Excellent Talent Support Program,China。
文摘Brassica napus is an important cash crop broadly grown for the vegetable and oil values.Yellow-seeded B.napus is preferred by breeders due to its improved oil and protein quality,less pigments and lignin compared with the blackseeded counterpart.This study compared the differences in flavonoid and fatty acid contents between yellow rapeseed from the progenies of B.napus-Sinapis alba somatic hybrids and the black-seeded counterpart using RNA-seq analysis.Through HPLC-PDA-ESI(-)/MSanalysis,it was found that phenylpropanoids and flavonoids(i.e.,isorhamnetin,epicatechin,kaempferol,and other derivatives)in yellow seed were significantly lower than those in black seed.The fatty acid(FA)content in yellow rapeseed was higher than that in black rapeseed due to the variation of C16:0,C18:0,C18:1,C18:2,and C18:3 contents.RNA-seq analysis of seeds at four and five weeks after flowering(WAF)indicated that differentially expressed genes(DEGs)between black and yellow rapeseeds were enriched in flavonoid and FA biosynthesis,including BnTT3,BnTT4,BnTT18,and BnFAD2.Also,genes related to FA biosynthesis,desaturation and elongation(FAD3,LEC1,FUS3,and LPAT2)in yellow seed were up-regulated compared to those in black seed,while genes involved in beta-oxidation cycle(AIM1 and KAT2)of yellow seed were down-regulated compared to those in black seed.The DEGs related to the variation of flavonoids,phenylpropanoids,and FAs would help improve the knowledge of yellow seed character in B.napus and promote rapeseed improvement.
文摘Improving grain amino acid content of rice(Oryza sativa L.)is essential for the health of consumers.This study was conducted to identify the physiological processes that contribute to the higher grain amino acid content in hybrid rice cultivar Lingliangyou 268 compared to Luliangyou 996.The results showed that total amino acid content in grains was 9%higher in Lingliangyou 268 than in Luliangyou 996.There was no significant difference in grain nitrogen(N)content between Lingliangyou 268 and Luliangyou 996,while ratio of amino acid to N was 6%higher in Lingliangyou 268 compared to Luliangyou 996.A total of 16 differentially expressed proteins related to amino acid metabolism(e.g.,erythronate-4-phosphate dehydrogenase domain containing protein)were identified in grains between Lingliangyou 268 and Luliangyou 996.The identified proteins were involved in 10 molecular functions.Six of the 10 defined functions were related to binding(heterocyclic compound binding,nucleoside phosphate binding,nucleotide binding,organic cyclic compound binding,protein binding,and small molecule binding)and the other 4 defined functions were catalytic activity,enzyme regulator activity,hydrolase activity,and transferase activity.These results indicate that the higher grain amino acid content in Lingliangyou 268 compared to Luliangyou 996 is attributed to increased efficiency of converting N to amino acid that results from altered expression of proteins related to amino acid metabolism.
基金supported by the National High Tech-nology Research and Development Program of China(2006AA10A114)the National Basic Research Program of China (2007CB116212)+1 种基金the Natural Science Fundation of Shangdong Province, China(ZR2009DQ004)the Key Technology Research Project of Qingdao, China (07-1-4-16-nsh)
文摘Phosphoenolpyruvate carboxylase (PEPC) is widely distributed in plants and bacteria, and catalyzes the carboxylation of phosphoenolpyruvate to form oxaloacetate and inorganic phosphate. To investigate the molecular mechanisms of the regulation and control of peanut oil, with the degenerated primers and RACE-PCR approach, five PEPC genes were cloned from peanut, and designated as AhPEPC1, AhPEPC2, AhPEPC3, AhPEPC4, and AhPEPC5, respectively. The structure and phylogenetic analysis of PEPC protein indicated that AhPEPC1-4 genes encoded a typical plant-type PEPC-enzyme, and AhPEPC5 a bacterial-type. By real-time quantitative RT-PCR approach the expression pattern of each gene was detected in various tissues of normal and high oil-content peanut varieties. It was found that there was a lower expression level of AhPEPCs genes except for the AhPEPC2 in high-oil peanut than normal-oil peanut line. The results provide some fundamental information for the further investigation of plant PEPC proteins and their role in regulation of oil-content in peanut seeds.