Eukaryotic food sources analysis in situ of tropical common sea cucumber Holothuria leucospilota based on 18S rRNA gene high-throughput sequencing

Sea cucumber Holothuria leucospilota is one of the most widespread tropical holothurian species.In this study,eukaryotic organism composition in foregut and hindgut contents of H.leucospilota and surrounding sediments was assessed by 18S rRNA gene high-throughput sequencing.Eukaryon richness and diversity in the habitat sediments were significantly higher than those in foregut and hindgut contents of the sea cucumbers(P<0.05).The foregut content group,hindgut content group,and marine sediment group sequences were respectively assigned to 18.20±1.32,19.40±1.03,and 21.80±0.37 phyla.In the foregut contents,Nematoda(20.18%±9.59%),Mollusca(16.12%±10.49%),Chlorophyta(10.04%±4.85%),Annelida(8.72%±10.93%),Streptophyta(8.46%±4.65%),and Diatomea(5.99%±2.01%)were the predominant phyla,which showed the eukaryotic food sources of H.leucospilota were primarily belong to the above phyla.The predominant phyla in the hindgut contents were Streptophyta(45.55%±17.32%),Mollusca(4.93%±4.82%),Arthropoda(5.37%±3.08%),Diatomea(3.88%±2.34%),and Chlorophyta(3.79%±1.59%);and Annelida(37.80%±17.00%),Arthropoda(24.49%±12.53%),Platyhelminthes(7.14%±3.02%),Nematoda(4.14%±0.91%),and Diatomea(5.11%±1.35%)had large contents in the sediments.The comparatively high content of Paris genus in phylum Streptophyta in foregut contents indicated that land plants were one of the primary food sources of H.leucospilota,however the significantly higher contents of Streptophyta in hindgut contents than that in foregut contents might suggest a large part of the terrigenous detritus ingested might not be digested by H.leucospilota.UPGMA and PCoA analysis revealed that eukaryotic organism composition differed significantly between foregut contents of H.leucospilota and ambient sediments,indicating selective feeding feature of H.leucospilota.This study provided useful references for artificial feed of tropical sea cucumbers and enhanced understanding of the ecological roles of detritus-feeding macrobenthos.

Study of Quality Standards of Xiao’er Qingre Enema Preparation in Hospitals

Objective:To establish the quality standards for the preparation of Xiao’er Qingre enema in hospitals.Method:Thin-layer chromatography(TLC)was used to identify Radix Isatidis and Glycyrrhizae in the prescription.The content of(R,S)-Goitrin was determined by high-performance liquid chromatography(HPLC).Results:In TLC identification,there was no interference between the negative controls of Radix Isatidis and glycyrrhizae,and the spots were well-separated.The HPLC results of(R,S)-Goitrin showed a good linear relationship between the peak area and concentration within the range of 0.476-95.2μg·mL-1(r=0.9999).Conclusion:The TLC and HPLC methods established in this experiment are simple,reproducible,and specific,making them suitable for the quality control Xiao’er Qingre enema preparation in hospitals.

基于S-YOLO V5和Vision Transformer的视频内容描述算法

视频内容描述的自动生成是结合计算机视觉和自然语言处理等相关技术提出的一种新型交叉学习任务。针对当前视频内容生成描述模型可读性不佳的问题,本研究提出一种基于S-YOLO V5和Vison Transformer(ViT)的视频内容描述算法。首先,基于神经网络模型KATNA提取关键帧,以最少帧数进行模型训练;其次,利用S-YOLO V5模型提取视频帧中的语义信息,并结合预训练ResNet101模型和预训练C3D模型提取视频静态视觉特征和动态视觉特征,并对两种模态特征进行融合;然后,基于ViT结构的强大长距离编码能力,构建模型编码器对融合特征进行长距离依赖编码;最后,将编码器的输出作为LSTM解码器的输入,依次输出预测词,生成最终的自然语言描述。通过在MSR-VTT数据集上进行测试,本研究模型的BLEU-4、METEOR、ROUGEL和CIDEr分别为42.9、28.8、62.4和51.4;在MSVD数据集上进行测试,本研究模型的BLEU-4、METEOR、ROUGEL和CIDEr分别为56.8、37.6、74.5以及98.5。与当前主流模型相比,本研究模型在多项评价指标上表现优异。

Influence of S Contents on the Hydrogen Blistering and Hydrogen Induced Cracking of A350LF2 Steel

In the present work, the effects of chemical compositions on the hydrogen blistering and hydrogen induced cracking of A350LF2 steel with different S contents were studied. Four types of A350LF2 steels were evaluated by immersing samples in H2S-saturated NACE solution. The hydrogen blistering, crack length rate(CLR), crack thickness rate(CTR) and crack sensitivity rate(CSR) were evaluated. The results show that there are many hydrogen blisters on the sample surface with S content of 0.021%, a few on the sample surface with S content of 0.019% and 0.012% and no one on the surface with S content of 0.002%. There were 12, 2 and 1 strips of cracks of longer than 0.3 mm on the evaluated cross sections with S content of 0.021%, 0.019% and 0.012%, respectively. There was no any crack in the sample with 0.002% S. The corrosion rate was also evaluated. The S content has no obvious influence on the corrosion rate.

Information content method for evaluating diagnostic tests and its comparison of power with other methods

Ｉｎｆｏｒｍａｔｉｏｎｃｏｎｔｅｎｔｍｅｔｈｏｄｆｏｒｅｖａｌｕａｔｉｎｇｄｉａｇｎｏｓｔｉｃｔｅｓｔｓａｎｄｉｔｓｃｏｍｐａｒｉｓｏｎｏｆｐｏｗｅｒｗｉｔｈｏｔｈｅｒ ｍｅｔｈｏｄｓＣｈｅｎＰｉｎｇｙａｎ（陈平雁）；ＷａｎｇＢｉｎｈｕｉ（王斌会）；...

Effect of H_(2)S content on relative permeability and capillary pressure characteristics of acid gas/brine/rock systems:A review

Geological storage of acid gas has been identified as a promising approach to reduce atmospheric carbon dioxide(CO_(2)),hydrogen sulfide(H_(2)S)and alleviate public concern resulting from the sour gas production.A good understanding of the relative permeability and capillary pressure characteristics is crucial to predict the process of acid gas injection and migration.The prediction of injection and redistribution of acid gas is important to determine storage capacity,formation pressure,plume extent,shape,and leakage potential.Herein,the existing experimental data and theoretical models were reviewed to gain a better understanding of the issue how the H_(2)S content affects gas density,gas viscosity,interfacial tension,wettability,relative permeability and capillary pressure characteristics of acid gas/brine/rock systems.The densities and viscosities of the acid gas with different H_(2)S mole fractions are both temperature-and pressure-dependent,which vary among the gas,liquid and supercritical phases.Water/acid gas interfacial tension decreases strongly with increasing H_(2)S content.For mica and clean quartz,water contact angle increases with increasing H_(2)S mole fraction.In particular,wettability reversal of mica to a H_(2)S-wet behavior occurs in the presence of dense H_(2)S.The capillary pressure increases with decreasing contact angle.At a given saturation,the relative permeability of a fluid is higher when the fluid is nonwetting.The capillary pressure decreases with decreasing interfacial tension at a given saturation.However,the existing datasets do not show a consistent link between capillary number and relative permeability.The capillary pressure decreases with increasing H_(2)S mole fraction.However,there is no consensus on the effect of the H_(2)S content on the relative permeability curves.This may be due to the limited availability of the relative permeability and capillary pressure data for acid gas/brine/rock systems;thus,more experimental measurements are required.

Influence of Carbon Content on S Zorb Sorbent Activity

The reaction activity of S Zorb sorbents with different sulfur contents was investigated, and the structure and composition of carbon-containing sorbents were characterized by XRD, FT-IR and TG-MS in order to delve into the kind and morphology of carbon on the sorbent. Test results have revealed that coke could be deposited on the S Zorb sorbent during the operating process, and the coke content was an important factor influencing the reaction performance of the S Zorb sorbent. Retention of a definite amount of coke on the sorbent while securing the desulfurization activity of the S Zorb sorbent would be conducive to the reduction of octane loss of reaction product.

Antioxidant Activity and Phenolic Compounds Content of Extracts from Ceratotheca sesamoides Endl and Striga hermonthica (Delille) Benth Used as Antheminthic in Burkina Faso

The revaluation of medicinal plants in the veterinary pharmacopoeia is essential for the development of livestock in Burkina Faso. In order to justify the use of C. sesamoides and S. hermonthica in the treatment of gastrointestinal parasitosis of small ruminants, a quantification of phenolic compounds as well as antioxidant activity and acute toxicité evaluation of both plants were performed. Acute toxicity was evaluated by administering a single dose of 2000 mg/kg body weight of aqueous extract of both plants to mice. The highest total polyphenol content for C. sesamo?des was obtained in the ethyl acetate fraction with 47.236 ± 2.57 mgEAG/100mg while that of S. hermonthica was 74.871 ± 2.57 mgEAG/100mg obtained with the n-butanol Fraction. The dichloromethane extract of S. hermonthica obtained the highest total flavonoid content with 7.31 ± 0.48 mgEQ/100 while the highest total flavonoid content of C. sesamo?des was obtained with ethyl acetate fraction with 5.4273 ± 0.35 mgEQ/100mg. The aqueous extract of C. sesamo?des obtained the highest content of condensed tannins with 3.028 ± 0.13 mgEAT/100mg. Both plant’s extracts did not show any signs of toxicity in NMRI mice after administration of the dose of 2000 mg/kg. The antioxidant activity by DPPH, FRAP and ABTS methods was good compared to those of Quercetin and Trolox taken as standard.

Antioxidant and Phenolic Content of Nuts, Oil Seeds, Milk and Milk Products Commonly Consumed in India

Food provides not only essential nutrients required for life, but also bioactive compounds usefull to maintan good health and disease prevention. Abundant epidemiological evidences suggest that consumption of food rich in antioxi-dants (non-nutritional components) can prevent degenerative diseases. A total of 26 commonly consumed nuts, oil seeds, edible oils, milk and milk products were chosen for the study. Considering the fact that antioxidant content (AOC) and phenolic contents (PC) of these foods was not established systematically in Indian context. Therefore, we have assessed and correlated the AOC and PC, an important antioxidant constituents of plant foods. AOC was assessed by DPPH (2, 2-Diphenyl-1-picryl hydrazyl) scavenging activity and FRAP (Ferric reducing antioxidant power) methods and phenolic content (PC), using Folin-Ciocalteu reagent. Among the nuts and oil seeds arecanut had the highest phenolic and antioxidant content 10841, 4220341 mg/100g respectively. In milk, edible oils and sugars the values ranged from 336 -11674 mg/100g. Jaggery had the highest PC and AOC among the foods studied . Although AOC and PC showed wide variation among the foods, AOC was correlated significantly with PC. Indeed the ‘r’ value between PC and AOC (DPPH and FRAP) was 0.99 (p < 0.01) among the nuts and oil seeds, while in milk, milk products and sugars, the “r” values ranged from 0.93 and 0.99 (p < 0.01) respectively. The overall results indicate that the phenolic compounds may be significant contributors to the AOC of the foods studied.

Determination of the Content of Total Saponins in Paris daliensis H.Li et V. G. Soukoup and Paris dulongensis H. Li et S. kurita

[Objectives]To determine the content of total saponins in Paris daliensis H. Li et V. G. Soukoup and Paris dulongensis H. Li et S.kurita,fill the gap in the study of Paris daliensis H. Li et V. G. Soukoup and Paris dulongensis H. Li et S. kurita,and lay a foundation for searching new medicinal resources of Paris L. [Methods]The perchloric acid colorimetric method and UV spectrophotometer were used to determine the content of total saponins in Paris daliensis H. Li et V. G. Soukoup and Paris dulongensis H. Li et S. kurita at 406 nm. [Results]The lowest content of total saponins in Paris daliensis H. Li et V. G. Soukoup was 2. 637%,while the highest content reached 5. 474%; the lowest content of total saponins in Paris dulongensis was 1. 806%,while the highest content reached 3. 532%. Through the methodological examination,it was found that this method was stable and reliable. [Conclusions] The content of total saponins was high in Paris daliensis H.Li et V. G. Soukoup and Paris dulongensis H. Li et S. kurita,and it was suggested to further study and find the medicinal resources of Paris L.

A Content-Based Parallel Image Retrieval System on Cluster Architectures

We propose a content-based parallel image retrieval system to achieve high responding ability. Our system is developed on cluster architectures. It has several retrieval. servers to supply the service of content-based image retrieval. It adopts the Browser/Server (B/S) mode. The users could visit our system though web pages. It uses the symmetrical color-spatial features (SCSF) to represent the content of an image. The SCSF is effective and efficient for image matching because it is independent of image distortion such as rotation and flip as well as it increases the matching accuracy. The SCSF was organized by M-tree, which could speedup the searching procedure. Our experiments show that the image matching is quickly and efficiently with the use of SCSF. And with the support of several retrieval servers, the system could respond to many users at mean time. Key words content-based image retrieval - cluster architecture - color-spatial feature - B/S mode - task parallel - WWW - Internet CLC number TP391 Foundation item: Supported by the National Natural Science Foundation of China (60173058)Biography: ZHOU Bing (1975-), male, Ph. D candidate, reseach direction: data mining, content-based image retrieval.

An Improved 6S Code for Atmospheric Correction Based on Water Vapor Content

Water vapor content in the atmosphere is very significant for atmospheric correction of optical remote sensing data. Nowadays, the common atmospheric correction models use a single value of the average water vapor content of the study area to perform atmospheric correction. As the distribution of water vapor content varies greatly with time and space, it is obviously inaccurate to represent the total water vapor conditions of the whole area by just reading the average water vapor content. In this study, we altered the 6S sources so that it could read the water vapor content image which was retrieved from MODIS 1 km data. Atmospheric correction was implemented for the band 1 of MODIS 500 m data pixel-by-pixel using the improved 6S model. In comparison with the traditional 6S model, this improved 6S model is more reasonable in atmospheric correction, for it considers the spatial distribution of the water vapor content retrieved from MODIS data in the near infrared to define the atmospheric conditions for simulating the atmospheric radiative transfer. The results corrected by the improved 6S model showed more reasonable in pixel spatial distribution and closer histogram with the original image than those by traditional 6S model.

Influence of water content on the inactivation of P. digitatum spores using an air–water plasma jet

In order to investigate whether an air–water plasma jet is beneficial to improve the efficiency of inactivation, a series of experiments were done using a ring-needle plasma jet. The water content in the working gas（air） was accurately measured based on the Karl Fischer method. The effects of water on the production of OH（A;Σ;–X;Π;） and O（3p;P–3s;S） were also studied by optical emission spectroscopy. The results show that the water content is in the range of 2.53–9.58 mg l;, depending on the gas/water mixture ratio. The production of OH（A;Σ;–X;Π;） rises with the increase of water content, whereas the O（3p;P–3s;S） shows a declining tendency with higher water content. The sterilization experiments indicate that this air–water plasma jet inactivates the P. digitatum spores very effectively and its efficiency rises with the increase of the water content. It is possible that OH（A;Σ;–X;Π;） is a more effective species in inactivation than O（3p;P–3s;S） and the water content benefit the spore germination inhibition through rising the OH（A;Σ;–X;Π;） production. The maximum of the inactivation efficacy is up to 93% when the applied voltage is -6.75 kV and the water content is 9.58 mg l;.

Vital Contents of China's Foreign Trade in 2007

No.1-2 SPECLAL REPORT Sino-African Trade Cooperation China Should Attach Importance to GNP as well as GDP Foreign Trade in 2007,Stable and Rapid Development Forecasted Governor Punjab,Pakistan: Pakistan-China Friend-

Evaluation of Uncertainty in Measuring the Content of CaM V35S Promoter in Genetically Modified Soybean,GTS40-3-2,by Real-time Quantitative PCR

[ Objective ] This study aimed to investigate the major contributors to the measurement uncertainty in quantitative analysis of genetically modified ingreclients and improve the quality of quantitative detection of genetically modified components. [ Method] The content of CaMV35S promoter （parameter） in GTS40- 3-2 soybean powder samples was measured to estimate the measurement uncertainty preliminarily. [ Result] Type A uncertainty （uA） ＇ type B uncertainty （uB） and combined standard uncertainty （Uc） were 0.0 004, 0.002 and 0.002, respectively. At a confidence level ofp = 95% and freedom degree of Voff = 3 251, coverage factor k = 1.96, expanded uncertainty U = 0.004. The final measurement result was C = 0.028 ± 0. 004, which was dose to the conventional true value （0.03）. Thus, the measurement uncertainty was relatively small, indicating a high quality of measurement. In this study, uncertainty evaluation indicated that the deviation of micro liquid transfer made the greatest contribution to the measurement uncertainty. [ Cludusion ] The deviation of micro liquid transfer should be reduced to im- prove the quality of measurement.