Summary: A three-dimensional (3D) graphic model of a single-chain Fv (scFv) which was derived from an anti-human placental acidic isoferritin (PAF) monoclonal antibody (MAb) was construct- ed by a homologous protein...Summary: A three-dimensional (3D) graphic model of a single-chain Fv (scFv) which was derived from an anti-human placental acidic isoferritin (PAF) monoclonal antibody (MAb) was construct- ed by a homologous protein-predicting computer algorithm on Silicon graphic computer station. The structure, surface static electricity and hydrophobicity of scFv were investigated. Computer graphic modelling indicated that all regions of scFv including the linker, variable regions of the heavy (VH) and light (VL) chains were suitable. The VH region and the VL region were involved in composing the 'hydrophobic pocket'. The linker was drifted away VH and VL regions. The complementarity determining regions (CDRs) of VH and VL regions surrounded the 'hydrophobic pocket'. This study provides a theory basis for improving antibody affinity, investigating antibody structure and analyzing the functions of VH and VL regions in antibody activity.展开更多
From the point of constructing e-commerce application system, based on the structured analysis and Object-Oriented Design method, a combined modeling method Business-Process Driven(BPD) is proposed. This method focuse...From the point of constructing e-commerce application system, based on the structured analysis and Object-Oriented Design method, a combined modeling method Business-Process Driven(BPD) is proposed. This method focuses on the business process through the development process of the system. First, the business model of the system, then commercial object model is introduced according to the business model. At last the COM-model for the system is established. The system is implemented in an iterative and incremental way. The design and analysis result of each stage is illustrated by series of views using the modeling tool UML.展开更多
文摘Summary: A three-dimensional (3D) graphic model of a single-chain Fv (scFv) which was derived from an anti-human placental acidic isoferritin (PAF) monoclonal antibody (MAb) was construct- ed by a homologous protein-predicting computer algorithm on Silicon graphic computer station. The structure, surface static electricity and hydrophobicity of scFv were investigated. Computer graphic modelling indicated that all regions of scFv including the linker, variable regions of the heavy (VH) and light (VL) chains were suitable. The VH region and the VL region were involved in composing the 'hydrophobic pocket'. The linker was drifted away VH and VL regions. The complementarity determining regions (CDRs) of VH and VL regions surrounded the 'hydrophobic pocket'. This study provides a theory basis for improving antibody affinity, investigating antibody structure and analyzing the functions of VH and VL regions in antibody activity.
文摘From the point of constructing e-commerce application system, based on the structured analysis and Object-Oriented Design method, a combined modeling method Business-Process Driven(BPD) is proposed. This method focuses on the business process through the development process of the system. First, the business model of the system, then commercial object model is introduced according to the business model. At last the COM-model for the system is established. The system is implemented in an iterative and incremental way. The design and analysis result of each stage is illustrated by series of views using the modeling tool UML.