To investigate the effects of long-term fertilization systems on soil microbial community structure,labile organic carbon and nitrogen and enzyme activity in yellow sand paddy field and upland,a field experiment was c...To investigate the effects of long-term fertilization systems on soil microbial community structure,labile organic carbon and nitrogen and enzyme activity in yellow sand paddy field and upland,a field experiment was conducted at the experimental station of Dongyang Institute of Maize Research in Zhejiang Province,China in 2009.The experiment consisted of six treatments with three replicates,and they were arranged in a completely randomized design,including no fertilization in paddy field (PCK),conventional fertilization in paddy field (PCF),formulated fertilization by soil testing in paddy field (PSTF),formulated fertilization by soil testing with organic manure in paddy field (PSTF+OF),conventional fertilization on upland (DCF),and formulated fertilization by soil testing with organic manure on upland (DSTF+OF).Soil nutrients,enzyme activity,microbial biomass and community structure were determined in 2015.The results showed that compared with no fertilization in paddy field (PCK),fertilization increased soil phosphorus and potassium content,and decreased pH value.No fertilization in paddy field (PCK) had no significant effect on soil culturable microorganisms in paddy field and upland,but formulated fertilization by soil testing with organic manure on upland (DSTF+OF) significantly increased the number of fungi.Formula fertilization by soil testing with organic manure (PSTF+OF) also significantly increased soil microbial biomass carbon and nitrogen in paddy field and upland.Moreover,fertilization had no significant effect on soil cellulase activity,but formula fertilization by soil testing with organic manure (PSTF+OF) significantly increased soil dehydrogenase and catalase activity.Therefore,long-term application of chemical fertilizer with organic fertilizer can effectively improve soil fertility.展开更多
Effects of different levels of compost application on the amounts and percentage distribution of organic N forms in whole soils and particle size fractions were investigated. Soil samples were collected from three plo...Effects of different levels of compost application on the amounts and percentage distribution of organic N forms in whole soils and particle size fractions were investigated. Soil samples were collected from three plots: (a) F, only chemical fertilizers;(b) F+LC, chemical fertilizers plus low level of compost;(c) F+HC, chemical fertilizers plus high level of compost. Each soil sample was divided into five fractions: coarse sand-sized aggregate (CSA), medium sand-sized aggregate (MSA), fine sand-sized aggregate (FSA), silt-sized aggregate (SIA) and clay-sized aggregate (CLA) fractions. The sand fractions were subdivided into decayed plants (DP) and mineral particles (MP). The amounts of total N and different organic N forms in the whole soils as well as size fractions generally increased with increasing the amount of compost. In the whole soils, percentage distribution of non-hydrolysable-N and amino sugar-N increased by compost application while the distribution values of the hydrolysable ammonium- N and unidentified-N decreased. The application did not affect the distribution degree of amino acid-N. In the size fractions, the distribution values of most organic N forms increased in the CSA-DP, MSA-DP and FSA-DP fractions by compost application. In the CLA fractions, the amounts and percentage distribution of organic N forms were the highest, although the application caused decreases in their distribution values. These findings indicate that the CLA fraction merit close attention as an important reservoir of various organic N.展开更多
基金Supported by National Modern Agricultural Industrial Technology System(CARS-02-69)Major Agriculture Science Foundation of Upland Grain Crops Breeding of Zhejiang Province(2016C02050-9-1)Project for Training of Youth Talents of Zhejiang Academy of Agricultural Sciences(2015)
文摘To investigate the effects of long-term fertilization systems on soil microbial community structure,labile organic carbon and nitrogen and enzyme activity in yellow sand paddy field and upland,a field experiment was conducted at the experimental station of Dongyang Institute of Maize Research in Zhejiang Province,China in 2009.The experiment consisted of six treatments with three replicates,and they were arranged in a completely randomized design,including no fertilization in paddy field (PCK),conventional fertilization in paddy field (PCF),formulated fertilization by soil testing in paddy field (PSTF),formulated fertilization by soil testing with organic manure in paddy field (PSTF+OF),conventional fertilization on upland (DCF),and formulated fertilization by soil testing with organic manure on upland (DSTF+OF).Soil nutrients,enzyme activity,microbial biomass and community structure were determined in 2015.The results showed that compared with no fertilization in paddy field (PCK),fertilization increased soil phosphorus and potassium content,and decreased pH value.No fertilization in paddy field (PCK) had no significant effect on soil culturable microorganisms in paddy field and upland,but formulated fertilization by soil testing with organic manure on upland (DSTF+OF) significantly increased the number of fungi.Formula fertilization by soil testing with organic manure (PSTF+OF) also significantly increased soil microbial biomass carbon and nitrogen in paddy field and upland.Moreover,fertilization had no significant effect on soil cellulase activity,but formula fertilization by soil testing with organic manure (PSTF+OF) significantly increased soil dehydrogenase and catalase activity.Therefore,long-term application of chemical fertilizer with organic fertilizer can effectively improve soil fertility.
文摘Effects of different levels of compost application on the amounts and percentage distribution of organic N forms in whole soils and particle size fractions were investigated. Soil samples were collected from three plots: (a) F, only chemical fertilizers;(b) F+LC, chemical fertilizers plus low level of compost;(c) F+HC, chemical fertilizers plus high level of compost. Each soil sample was divided into five fractions: coarse sand-sized aggregate (CSA), medium sand-sized aggregate (MSA), fine sand-sized aggregate (FSA), silt-sized aggregate (SIA) and clay-sized aggregate (CLA) fractions. The sand fractions were subdivided into decayed plants (DP) and mineral particles (MP). The amounts of total N and different organic N forms in the whole soils as well as size fractions generally increased with increasing the amount of compost. In the whole soils, percentage distribution of non-hydrolysable-N and amino sugar-N increased by compost application while the distribution values of the hydrolysable ammonium- N and unidentified-N decreased. The application did not affect the distribution degree of amino acid-N. In the size fractions, the distribution values of most organic N forms increased in the CSA-DP, MSA-DP and FSA-DP fractions by compost application. In the CLA fractions, the amounts and percentage distribution of organic N forms were the highest, although the application caused decreases in their distribution values. These findings indicate that the CLA fraction merit close attention as an important reservoir of various organic N.