Detection of Exogenous Gene Copies in Transgenic Soybean by Taqman Quantitative PCR Technique

[Objective] Taqman Quantitative PCR technique was adopted to detect the copies of exogenous nos terminator in transgenic hybrid soybean.[Method] With soybean Lectin as the endogenous reference gene,and gene complex DNA in non-GMO soybeans as the endogenous reference standard,the method of gradient dilution was used for separately calculate Ct value of endogenous reference gene and plasmid DNA and correlation standard curve equation of logarithm of copies,and then to calculate the copies of samples through substituting thus-obtained Ct into the standard curve equation.[Result] The standard curve equation of endogenous reference gene is y=-3.422x＋35.201,R2=0.998;and the standard curve equation of exogenous gene is y=-3.348x＋34.890,R2=0.999.Nos terminator and its lower boundary sequences in transgenic soybean is of single copy.[Conclusion] The study has provided a theoretical basis for determining exogenous gene copies in transgenic soybean.

ASYMPTOTICALLY ISOMETRIC COPIES OF l_P (1≤P<∞) AND c_0 IN BANACH SPACES

Let X be a Banach space. If there exists a quotient space of X which is asymptotically isometric to l^1, then X contains complemented asymptotically isometric copies of l^1. Every infinite dimensional closed subspace of l1. contains a complemented subspace of l1 which is asymptotically isometric to l1. Let X be a separable Banach space such that X^＊ contains asymptotically isometric copies of lp （1 〈 p 〈∞）. Then there exists a quotient space of X which is asymptotically isometric to lq （1/p ＋ 1/q=1）. Complemented asymptotically isometric copies of co in K（X, Y） and W（X, Y） are discussed. Let X be a Gelfand-Phillips space. If X contains asymptotically isometric copies of co, it has to contain complemented asymptotically isometric copies of co.

An analysis of silver candlesticks from a casting point of view:originals,copies,forgeries

Collecting silver artefacts has traditionally been a very popular hobby.Silver is addictive,therefore the number of potential collectors and investors appears to grow each year.Unfortunately,increases in the interest and buying potentials resulted in a number of forgeries manufactured and introduced to the open antique market.The items such as early silver candlesticks dictate a very high price,for many high quality fakes show very good appearances and matching similarities with originals.Such copies are traditionally manufactured by casting using the original items as patterns.Small details and variances in design features,position and shape of hallmarks,including the final surface quality are usual features to distinguish the fakes from the originals.This paper presents results of a study conducted on several silver candlesticks,including two artefacts bearing features of those produced in the mid 18th century,one original Italian candelabrum from Fascist era,and small candlesticks made in the early 20th century.Also,the paper presents some interesting contemporary coins-replicas of many those produced in different countries.The coins were offered for sale by unscrupulous dealers via auctions and e-bays.Finally the main results and findings from this study are discussed from a manufacturing point of view,such as fabrication technology,surface quality and hallmarks,which will help the collectors,dealers and investors to detect and avoid forgeries.

“Lion King”sells 3 million copies in return to video

Simba has come out roaring again in itsDVD debut. “The Lion King”sold 3 million copies in the first twodays of its new home-video release,distributor Disney saidThursday.

Degree Conditions on Copies of Forests in Graphs

For a graph G,letμ(G)=min{max{d(x),d(y)}:x≠y,xy∉E(G),x,y∈V(G)}if G is non-complete,otherwise,μ(G)=+∞.For a given positive number s,we call that a graph G satisfies Fan-type conditions ifμ(G)≥s.Supposeμ(G)≥s,then a vertex v is called a small vertex if the degree of v in G is less than s.In this paper,we prove that for a forest F with m edges,if G is a graph of order n≥|F|andμ(G)≥m with at most max{n−2m,0}small vertices,then G contains a copy of F.We also give examples to illustrate both the bounds in our result are best possible.

Asymptotically Isometric Copies of l^∞ in Some Banach Lattices

In this paper, we show that any a-complete Banach lattice, with a σ-order semicontinuous but not σ-order continuous norm, contains an asymptotically isometric copy of l^∞. We also get that the Fenchel-Orlicz space with the Orlicz norm may not contain an asymptotically isometric copy of l^∞.

On Asymptotically Isometric Copies of l~β(0 < β < 1)

We get the characterizations of the family of all nonnegative, subadditive,β-absolutely homogeneous and continuous functionals defined on X, when the ;3-normed space X contains an asymptotically isometric copy of l^β. Moreover, it is proved that if a closed bounded β-convex subset K of a β-normed space contains an asymptotically isometric β-basis, then K contains a closed β-convex subset C which fails the fixed point property.

Genome-wide investigation to assess copy number variants in the Italian local chicken population

Background Copy number variants(CNV)hold significant functional and evolutionary importance.Numerous ongoing CNV studies aim to elucidate the etiology of human diseases and gain insights into the population structure of livestock.High-density chips have enabled the detection of CNV with increased resolution,leading to the identification of even small CNV.This study aimed to identify CNV in local Italian chicken breeds and investigate their distribution across the genome.Results Copy number variants were mainly distributed across the first six chromosomes and primarily associated with loss type CNV.The majority of CNV in the investigated breeds were of types 0 and 1,and the minimum length of CNV was significantly larger than that reported in previous studies.Interestingly,a high proportion of the length of chromosome 16 was covered by copy number variation regions(CNVR),with the major histocompatibility complex being the likely cause.Among the genes identified within CNVR,only those present in at least five animals across breeds(n=95)were discussed to reduce the focus on redundant CNV.Some of these genes have been associated to functional traits in chickens.Notably,several CNVR on different chromosomes harbor genes related to muscle development,tissue-specific biological processes,heat stress resistance,and immune response.Quantitative trait loci(QTL)were also analyzed to investigate potential overlapping with the identified CNVR:54 out of the 95 gene-containing regions overlapped with 428 QTL associated to body weight and size,carcass characteristics,egg production,egg components,fat deposition,and feed intake.Conclusions The genomic phenomena reported in this study that can cause changes in the distribution of CNV within the genome over time and the comparison of these differences in CNVR of the local chicken breeds could help in preserving these genetic resources.

Sperm function, mitochondrial activity and in vivo fertility are associated to their mitochondrial DNA content in pigs

Background Despite their low abundance in sperm, mitochondria have diverse functions in this cell type, includ-ing energy production, signalling and calcium regulation. In humans, sperm mitochondrial DNA content(mtDNAc) has been reported to be negatively linked to sperm function and fertility. Yet, the association between mtDNAc and sperm function in livestock remains unexplored. For this reason, this study aimed to shed some light on the link between mtDNAc and sperm function and fertilising potential in pigs. A qPCR method for mtDNAc quantification was optimised for pig sperm, and the association of this parameter with sperm motility, kinematics, mitochondrial activity, and fertility was subsequently interrogated.Results First, the q PCR method was found to be sensitive and efficient for mtDNAc quantification in pig sperm. By using this technique, mtDNAc was observed to be associated to sperm motility, mitochondrial activity and in vivo, but not in vitro, fertility outcomes. Specifically, sperm with low mtDNAc were seen to exhibit greater motility but decreased mitochondrial activity and intracellular reactive oxygen species. Interestingly, samples with lower mtD-NAc showed higher conception and farrowing rates, but similar in vitro fertilisation rates and embryo development, when compared to those with greater mtDNAc.Conclusions These findings enrich our comprehension of the association of mtDNAc with sperm biology, and lay the foundation for future research into employing this parameter as a molecular predictor for sperm function and fer-tility in livestock.

Exploring the interplay of T cell receptor-V gene copy numbers and major histocompatibility complex selection pressure in avian species:Insights into immune system evolution and reproductive investment

Birds,a fascinating and diverse group occupying various habitats worldwide,exhibit a wide range of life-history traits,reproductive methods,and migratory behaviors,all of which influence their immune systems.The association between major histocompatibility complex(MHC)genes and certain ecological factors in response to pathogen selection has been extensively studied;however,the role of the co-working molecule T cell receptor(TCR)remains poorly understood.This study aimed to analyze the copy numbers of TCR-V genes,the selection pressure(ωvalue)on MHC genes using available genomic data,and their potential ecological correlates across 93 species from 13 orders.The study was conducted using the publicly available genome data of birds.Our findings suggested that phylogeny influences the variability in TCR-V gene copy numbers and MHC selection pressure.The phylogenetic generalized least squares regression model revealed that TCR-Vαδcopy number and MHC-I selection pressure were positively associated with body mass.Clutch size was correlated with MHC selection pressure,and Migration was correlated with TCR-Vβcopy number.Further analyses revealed that the TCR-Vβcopy number was positively correlated with MHC-IIB selection pressure,while the TCR-Vγcopy number was negatively correlated with MHC-I peptide-binding region selection pressure.Our findings suggest that TCR-V diversity is significant in adaptive evolution and is related to species’life-history strategies and immunological defenses and provide valuable insights into the mechanisms underlying TCR-V gene duplication and MHC selection in avian species.

Structural variations in oil crops:Types,and roles on domestication and breeding

Structural variations(SVs),a newly discovered genetic variation,have gained increasing recognition for their importance,yet much about them remains unknown.With the completion of whole-genome sequencing projects in oil crops,more SVs have been identified,revealing their types,genomic distribution,and characteristics.These findings have demonstrated the crucial roles of SVs in regulating gene expression,driving trait innovation,facilitating domestication,making this an opportune time for a systematic review.We summarized the progress of SV-related studies in oil crops,focusing on the types of SVs and their mechanisms of occurrence,the strategies and methods for SV detection,and the SVs identified in oil crops such as rapeseed,soybean,peanut,and sesame.The various types of SVs,such as presence-absence variations(PAVs),copy number variations(CNVs),and homeologous exchanges(HEs),have been shown.Along with their genomic characterization,their roles in crop domestication and breeding,and regulatory impact on gene expression and agronomic traits have also been demonstrated.This review will provide an overview of the SV research process in oil crops,enabling researchers to quickly understand key information and apply this knowledge in future studies and crop breeding.

Clinical manifestations and the prenatal diagnosis of trisomy 7 mosaicism:Two case reports

BACKGROUND The clinical manifestations of trisomy 7 mosaicism are diverse and nonspecific,so prenatal diagnosis is very difficult.CASE SUMMARY Two pregnant women with abnormal prenatal screening results were included.One was a 22-year-old woman(G1P0).At 31st week of gestation,ultrasound revealed that the posterior horn of the left lateral ventricle was 10 mm and the right renal pelvis had a separation of 7 mm.The other pregnant woman was 33 years old(G2P1L1A0),and her fetus was found to have a cardiac malformation at the 24th week of gestation.Copy number variation sequencing,whole-exome sequencing and karyotype analysis were carried out after amniocentesis,and both fetuses were diagnosed with trisomy 7 mosaicism.After parental counseling,one woman continued the pregnancy,and the other woman terminated the pregnancy.CONCLUSION In trisomy 7 mosaicism,the low proportion of trisomy does not lead to abortion,but can result in abnormal fetal development,which can be detected via ultrasound.Therefore,clinicians need to pay more attention to various aspects of fetal growth and development,combining with imaging,cellular,molecular genetics and other methods to perform comprehensive evaluations of fetuses to provide more reliable genetic counseling for pregnant women.

Deciphering the role of FSCN family genes in cancer:a pan-cancer bioinformatics study

Background:The Fascin(FSCN)family,comprising actin-bundling proteins,plays vital roles in cytoskeletal reorganization and cell migration.FSCN1,FSCN2,and FSCN3 are implicated in cancer progression through cell motility,invasion,and metastasis.However,their specific contributions across different cancer types remain unclear.Methods:We conducted a pan-cancer bioinformatics analysis of FSCN genes using data from The Cancer Genome Atlas.This included differential expression patterns,copy number variations(CNVs),mutations,methylation status,and correlations with tumor mutational burden,microsatellite instability,and immune checkpoint molecule expression.Differential expression was analyzed using DESeq2,while CNV and mutation analyses utilized GISTIC2.0 and MuTect2.Methylation data were assessed using the Illumina Human Methylation 450K BeadChip.Results:FSCN1 and FSCN2 showed significant differential expression in multiple cancers,often correlating with poor prognosis.FSCN3 exhibited less variability but a protective role in certain contexts.CNV analysis indicated frequent gene gains in FSCN genes,correlating with increased expression.FSCN3 had a higher mutation rate,suggesting genetic instability.Methylation analysis showed hypomethylation of FSCN1 and FSCN2 in tumors compared to normal tissues,whereas FSCN3 had minor changes.Significant associations were found between FSCN gene expression and tumor mutational burden,microsatellite instability,and immune checkpoint molecules,suggesting their involvement in tumor immunogenicity and the immune microenvironment.Conclusions:This pan-cancer analysis highlights the multifaceted roles of FSCN genes in cancer biology,emphasizing their potential as biomarkers and therapeutic targets.FSCN1 and FSCN2 are associated with poor prognosis and aggressive phenotypes,while FSCN3 shows protective roles in specific contexts.These findings offer new avenues for cancer diagnosis and treatment,particularly in personalized medicine.Future studies should validate these findings and explore the underlying mechanisms to fully harness the clinical potential of FSCN family proteins in oncology.

Relationship Between Gene-Phenotype and Clinical Manifestations of Chromosomal Copy Number Variations Indicated by Non-Invasive Prenatal Testing

Objective:To analyze the clinical value of non-invasive prenatal testing(NIPT)in detecting chromosomal copy number variations(CNVs)and to explore the relationship between gene expression and clinical manifestations of chromosomal copy number variations.Methods:3551 naturally conceived singleton pregnant women who underwent NIPT were included in this study.The NIPT revealed abnormalities other than sex chromosome abnormalities and trisomy 13,18,and 21.Pregnant women with chromosome copy number variations underwent genetic counseling and prenatal ultrasound examination.Interventional prenatal diagnosis and chromosome microarray analysis(CMA)were performed.The clinical phenotypes and pregnancy outcomes of different prenatal diagnoses were analyzed.Additionally,a follow-up was conducted by telephone to track fetal development after birth,at six months,and one year post-birth.Results:A total of 53 cases among 3551 cases showed chromosomal copy number variation.Interventional prenatal diagnosis was performed in 36 cases:27 cases were negative and 8 were consistent with the NIPT test results.This indicates that NIPT’s positive predictive value(PPV)in CNVs is 22.22%.Conclusion:NIPT has certain clinical significance in screening chromosome copy number variations and is expected to become a routine screening for chromosomal microdeletions and microduplications.However,further interventional prenatal diagnosis is still needed to identify fetal CNVs.

Linux卷管理系统Snapshot技术的分析与研究

快照技术是产生在线热备份的重要技术手段,其实现相当复杂。文章对Linux内核的卷管理系统的快照技术进行了分析和研究,讨论了与快照技术相关的数据结构、源逻辑卷的写请求处理以及快照逻辑卷的读请求处理。

TCP/IP协议卸载技术性能与实现的研究

本文通过分析当前计算机系统主要通信瓶颈,建立模型分析其卸载后的性能,得出协议卸载与节点处理能力、网络带宽、网卡处理能力以及需要处理的应用程序、网络数据包的强度有关的结论,得出了他们之间理想状态下的最优解.建立并初步实现TOE通信平台,实现零拷贝,利用Linux开源特点实现相应的软控制程序并结合ML403开发板实现相应的硬处理功能,实现简单数据包的收发.

CoPy/C催化剂应用碱性介质氧还原催化的电化学性能(英文)

利用碳黑(Vulcan XC-72R)中加入硫酸钴和吡啶(Py)作为催化剂前驱体,经溶剂分散热处理构建了一类新型的高效氧还原CoPy/C复合催化剂.并运用循环伏安法(CV)和旋转圆盘电极(RDE)技术研究了不同Co含量的CoPy/C催化剂在碱性介质中对氧还原的电催化活性.结果表明:Co的存在对氧的催化剂活性位的形成有重要影响,800℃下所制备的10%Co30%Py/C(质量分数)复合催化剂表现出最佳的氧还原催化活性.以其制备的气体扩散电极在3.0 mol·L^(-1)KOH电解质溶液(O_2气氛)中0.014 V(相对于标准氧电极(RHE))即可产生明显的氧还原电流.同40%Py/C相比,10%Co30%Py/C催化氧还原的起峰电位正移了71 mV,同时表现出明显的极限扩散电流.在-0.16 V时电流密度达到最大值,电流密度为1.0 mA·cm^(-2),半波电位在-0.07 V.透射电镜分析表明所制备的碳黑载吡啶钴(10%Co30%Py/C)催化剂平均粒径为20 nm.

Isolation of Rice EPSP Synthase cDNA and Its Sequence Analysis and Copy Number Determination

In order to isolate the total cDNA of rice (Oryza sativa L.) epsps gene, RT-PCR was carried out with template of rice first-strand cDNA and primers designed according to rice EPSP synthase genomic sequence obtained in previous study. A 1 585-bp cDNA fragment was amplified and cloned. The 1 585-bp cDNA contains an open reading frame (ORF) comprising of 1 533 nucleotides (nt) which encodes a 511 residue polypepetides, including 67 amino acids chloroplast transit peptide and 444 amino acids EPSP synthase mature peptide. A comparison between the EPSP synthase of different sources indicates that the mature peptide shows more than 51% identity except for the fungi EPSP synthase and the transit peptide shows considerably less sequence conservation. The copy number of rice epsps gene is estimated to be one copy per haploid rice genome using southern blot. RT-PCR indicated that rice epsps gene is expressed in rice leaves, endosperms and roots and has the highest expression level in leaves.

COPYCAD软件在逆向工程中的应用

随着计算机技术和自动化测量技术的高速发展,逆向工程已经成为一种重要的设计手段,同时也出现了很多专门用于逆向工程的软件.本文通过对COPYCAD的介绍,展示了这些软件工作的一般步骤,总结了他们的一些共性.

加工中心在线测量系统在自由曲面COPY加工中的应用研究

在没有三坐标测量机等不具备逆向设计条件的情况下,通过编制用户宏指令对在线测量系统进行控制,收集和处理模型曲面测量数据,则可以实现类似三坐标测量机自动扫描曲面测量和实现仿形加工的功能。阐述了COPY加工的原理、程序的编制方法、加工误差分析及为保证加工精度应采取的措施。