Synthesis, Structure and Fluorescence of a Copper(II) Complex [Cu_2(bipy)_2(Hpht)_2Cl](Hpht)(bipy=2,2'-Bipyridine,H_2pht=o-Phthalic Acid)

The title complex [Cu2(bipy)2(Hpht)2Cl](Hpht) (bipy = 2,2?-bipyridine, H2pht = o-phthalic acid) has been synthesized in the NaOH aqueous solution of CuCl2, Gd(NO3)3, bipy and H2pht, and its crystal structure was determined by single-crystal X-ray diffraction method. It crys- tallizes in triclinic, space group P1, C44H31ClCu2N4O12, Mr = 970.26, a = 8.175(2), b = 16.254(4), c = 16.946(4) ?, α = 62.966(6), β = 84.833(8), γ = 84.348(8)°, V = 1993.4(8) ?3, Z = 2, Dc = 1.616 g/cm3, F(000) = 988 and μ = 1.207 mm-1. The final R = 0.0429 and wR = 0.0843 for 5682 observed reflections with I > 2σ(I). Each copper(II) atom displays a distorted square-pyramidal geometry with two nitrogen atoms of one chelate 2,2?-bipy molecule, two oxygen atoms from two different bridging carboxylate groups of Hpht- and one bridging chloride atom occupying the apical position. The two copper(II) atoms are connected by a μ2-Cl atom and two bridging Hpht- ligands in a syn-syn coordination mode to form an isolated dinuclear unit. The molecular structure is extended to a one-dimensional wavy chain through hydrogen bonds. The title complex exhibits blue fluorescent emission at 443 nm (λex = 372 nm) in the solid state at room temperature.

Synthesis, Structure and Spectral Characterization of a Discrete Binuclear Copper (II) Complex

A discrete binuclear copper (II) complex containing Cu 2O 2 unit has been synthesized by the reaction of Cu (ClO 4) 2·6H 2O with 2 aminopyridine in the CH 3OH solution and characterized by X ray diffraction study and IR spectroscopy. The crystal is monoclinic, space group: P2 1/n, with unit cell parameters: a=8.2952(2), b=18.4633(3), c=10.5049(2) . β=108.965(1)°, V=1521.56(5) 3, Z=2, C 22 H 30 N 8O 10 Cl 2Cu 2, M r =764.52, D c =1.669 Mg/m 3, F(000)=780, μ (Mo Kα )=8.60, T =293(2)K, final R=0.0623, wR =0.1536 for 2660 observed reflections with I>2.0σ(I ). The centrosymmetric complex Cu 2(CH 3O) 2(C 5H 6N 2) 4(ClO 4) 2 comprises a pair of Cu(II) atoms bridged by two methanoxo and N coordination 2 aminopyridine. The Cu(II)…Cu(II) distance is 3\^002(1).

Hydrothermal Synthesis and Structure of a Copper(II) Complex, [Cu(4,4'bpy)(H_2O)_3]SO_42H_2O

The title complex [Cu(4,4?bpy)(H2O)3]SO42H2O (Mr = 405.86) was synthesized under hydrothermal conditions and its crystal structure has been determined by X-ray diffraction. It crystallizes in the hexagonal system, space group P61 with a = 11.1870(4), c = 21.578(1) ? V = 2338.6(2) ?, Dc = 1.729 g/cm3, Z = 6, m(MoKa) = 1.583 mm-1 and F(000) = 1254. The final R and wR are 0.0253 and 0.0416 for 1458 observed reflections with I≥2s(I), respectively. It was revealed that the square pyramidal Cu(II) sites are linked through 4,4?bipyridine groups, forming infinite one-dimensional chains, with the sulfate anions and lattice water molecules occupying the inter-chain positions. During the electrostatic interactions, there exist O…O hydrogen bonds and p-p stacking interactions between the parallel aromatic bipy rings in the structure.

Synthesis and Crystal Structure of a New Copper(II) Complex with 4-Cyanobenzoic Acid

A new Cu（Ⅱ） complex [Cu（4-cba）（1,10-phen）（H2O）2]（NO3） （4-Hcba = 4-cyanobenzoic acid） has been synthesized by solvothermal reaction in an ethanol/water mixed solution at 100 ℃ and structurally characterized by single-crystal X-ray diffraction. Crystallographic data： C20H16CuN4O7, Mr= 487.91, triclinic, space group PI, a = 7.8420（2）, b = 9.1070（2）, c = 15.1140（6） A, a = 76.889（9）, β = 81.332（11）, γ = 74.844（ 11）°, V = 1009.89（5） A^3, Z = 2, Dc = 1.605 g/cm^3, F（000） = 498, μ = 1.134 mm^-1, the final R = 0.0379 and wR = 0.0865 for 2977 observed reflections with 1 〉 2σ（Ⅰ）. The Cu（Ⅱ） atom is coordinated by two terminal water molecules, one chelating 1,10-phen molecule and one monodentate 4-cba ligand to form a slightly distorted square pyramid. The title complex molecules are connected through hydrogen bonds and π-π stacking interactions to generate a 2D layered network. The thermogravimetric analysis of the title complex has also been discussed.

Synthesis, structure, DNA binding and cleavage activity of a new copper(II) complex of bispyridylpyrrolide

A copper-bispyridylpyrrolide complex [Cu(PDPH)Cl](PDPH = 2,5-bis(2′-pyridyl)pyrrole) was synthesized and characterized. The complex crystallizes in the orthorhombic system with space group Pccn, a = 0.9016(3) nm, b = 1.0931(4) nm, c =2.5319(8) nm, and V = 2.4951(15) nm3. The copper center is situated in a square planar geometry. The interaction of the copper(II)complex with calf thymus DNA(CT-DNA) was investigated by electronic absorption, circular dichroism(CD) and fluorescence spectra. It is proposed that the complex binds to CT-DNA through groove binding mode. Nuclease activity of the complex was also studied by gel electrophoresis method. The complex can efficiently cleave supercoiled p BR322 DNA in the presence of ascorbate(H2A) via oxidative pathway. The preliminary mechanism of DNA cleavage by the complex with different inhibiting reagents indicates that the hydroxyl radicals were involved as the active species in the DNA cleavage process.

Hydrothermal Synthesis,Crystal Structure and Electrochemical Properties of the Copper(II) Complex [Cu_4O_4(phen)_4(ClC_6H_4COO)_2(H_2O)_2]·(H_2O)_3

The title complex of copper（Ⅱ） with m-chlorobenzoic acid, 1,10-phenanthroline （phen） and copper perchlorate has been synthesized and characterized in the solvent mixture of water and methanol. Crystal data for this complex： triclinic, space group PI^-, a = 1.06853（12）, b = 1.30740（16）, c = 1.49546（17） nm, α= 101.791（2）,β = 103.413（2）, γ = 105.815（2）°, V= 1.8736（4） nm^3, Mr = 904.67, Dc = 1.604 g/cm^3, Z = 2,/7（000） = 924,μ = 1.34 mm^-1, GOOF = 1.049, the final R = 0.0324 and wR = 0.0797. The structure analysis shows that a chair-like structure [Cu4O4] is defined by three quadrilaterals shaped by four copper and four oxygen atoms, and every copper ion is coordinated by three oxygen atoms from three wate： molecules and two nitrogen atoms from one 1,10-phenanthroline molecule, giving a distorted square-pyramidal coordination geometry. The CV analysis results indicate that the electron transfer in the electrode reaction is quasi-reversible.

Synthesis and Crystal Structure of a Copper(II) Complex with 2,4-Dihydroxybenzylidene Benzoylhydrazone Ligand

A new copper（Ⅱ） complex with formula CuL（py）, where L^2- is the dianion of 2,4-dihydroxybenzylidene benzoylhydrazone, has been synthesized and characterized by IR spectra and single-crystal X-ray diffraction method. The crystal belongs to monoclinic, space group P2 1/c with a=0.9630（4）, b=1.4808（6）, c=1.2320（5） nm, β=104.969（6）°, V=1.6973（Ⅱ） nm^3, Z=4, Dc=1.553 g/cm^3,μ（MoKa）=1.311 mm^-1, F（000）=812, R=0.0694 and wR=0.1727 for 1511 observed reflections （I〉2σ（I））. The crystal structure analysis indicates that the copper（Ⅱ） ion lies in a distorted square-planar environment composed of two oxygen atoms, one nitrogen atom from the tridentate acylhydrazone Schiff base ligand L^2- and one nitrogen atom from the pyridine ligand. Two adjacent molecules are connected via weak Cu…O bonding interactions to form dimers, which are further linked to generate a two-dimensional layer structure via interdimeric hydrogen bonds.

Synthesis and Crystal Structure of a Copper(II) Complex with Benzilic Acid and 1,10-Phenanthroline

A new complex [Cu（phen）3][（C6H4）2C（OH）COO]2·6H2O was prepared by selfassembly of benzilic acid, 1,10-phenanthroline （phen）, and copper perchlorate. It crystallizes in triclinic, space group P1-, with a = 1.14661（17）, b = 1.6455（2）, c = 1.6457（2） nm, α= 74.779（2）, β = 74.904（3）, γ = 84.424（3）°, V = 2.8914（7） nm^3, Dc = 1.340 g/cm^3, Z = 2, F（000） = 1218, GOOF = 1.018, the final R = 0.0643 and wR= 0.1633. The crystal structure shows that the copper ion is coordinated with six nitrogen atoms from six 1,10-phenanthroline molecules, forming a distorted octahedral coordination geometry.

Synthesis,Structure and Properties of a Novel Copper(Ⅱ)-2,2'- biimidazole Complex-supported Compound:[Cu(H_2biim)_2]{[Cu(H_2biim)_2 ]_2[H_(10)W_(12)O_(44)]}·8H_2O

A new copper（II）-2,2＇-biimidazole metal-organic POMs-supported compound [Cu（H2biim）2]{[Cu（H2biim）212[H10W12O44]}＇8H2O （H2biim = 2,2＇-biimidazole） has been success- fully synthesized under hydrothermal conditions and characterized by single-crystal X-ray diffraction, IR spectroscopy, elemental analysis and TG. The crystal adopts space group Pi with a = 11.800（2）, b = 13.437（3）, c = 13.685（3） A, V = 1922.5（7） A3, D,. = 3.507 g/cm3, C36H62Cu3N24W12O52, M,. = 4059.92, F（000） = 1837,μ = 18.796 mm-1, Z = 1, R = 0.0579 and wR = 0.1465 for 8734 observed reflections （I 〉 2σ（I））. X-ray crystallography analysis exhibits that the title compound consists of one [H10W12044]6- polyanion and three [Cu（H2biim）2] units. And significant π-π stacking interactions and intermolecular O-H-,.O or N-H.＇-O hydrogen-bonding interactions lead to a three-dimensional supramolecular framework. Thermal stability and magnetic property of the complex have also been investigated.

Synthesis,Crystal Structure and Catalytic Performance of the Trifluoro-substituted Mono(β-diiminato)Copper(Ⅱ)Complex

Two novel copper complexes with methyl or trifluoro-substituted mono-β-diiminato ligands and one acetoxyl anion were synthesized and characterized by IR spectroscopy and elemental analysis for the first time. The structure of complex 2 bearing trifluoro substituents was further confirmed by single-crystal X-ray diffraction. The crystal belongs to the triclinic system, space group P1 with a = 7.377（4）, b = 11.727（6）, c = 12.913（7） ？, α = 116.569（6）, β = 98.829（7）, γ = 96.520（6）°, V = 966.2（8） ？3, Z = 2, Mr = 479.86, Dc = 1.649 g/cm3, F（000） = 482, μ = 1.205 mm-1, the final R = 0.0370 and w R = 0.0903 for 3430 observed reflections with I 〉 2σ（I）. These mono-β-diiminato copper complexes can effectively catalyze methacrylate（MA） polymerization when activated by MMAO. The introduction of fluoro groups into the N-aryl ring of β-diiminato ligands can greatly increase the catalytic activity of copper complexes as well as the molecular weight of PMA.

Synthesis and Crystal Structure of Copper(II) Complex with N-Acetoxyl-picolinamide

Using Cu(II) as the template, a complex {[Cu2L2(H2O)2]·4H2O}n (L = N-acetoxyl- picolinamide) has been successfully synthesized and characterized by single-crystal X-ray diffrac- tion. The crystal is of monoclinic, space group C2/c, with a = 24.144(5), b = 7.1622(14), c = 17.283(4) ?, C16H24Cu2N4O12, Mr = 591.47, β = 131.73(3)o, V = 2230.3(8) ?3, Z = 4, Dc = 1.761 g/cm3, F(000) = 1208, μ = 1.978 mm?1, R = 0.0400 and wR = 0.1099. The copper (II) ion is five- coordinated with a distorted square pyramidal geometry. The complex can be viewed as a one- dimensional chain structure by carboxylic bridges among copper atoms. In the complex there exist hydrogen bonding interactions to stabilize the structure.

Synthesis and Crystal Structure of the Copper(II) Complex with Tris-(1,10-phenanthroline-5,6-dione)

The title complex [CuL3]（ClO4）2·2H2O·2CH3CN （L = 1,10-phenanthroline-5,6-dione） has been synthesized and characterized by elemental analysis, conductivity, infrared and UV-Vis spectra. X-ray diffraction analysis at room temperature indicates that the complex （C40H28Cl2CuN8O16, Mr = 1011.14） crystallizes in orthorhombic system, space group P212121 with a=13.983（1）, b=14.310（1）, c= 20\^890（2） , V = 4179.7（6） 3, Z = 4, Dc = 1.607 g/cm3, F（000） = 2026,μ（MoKα） = 0.736 mm-1. The final R and wR factors are 0.0446 and 0.1212 respectively with 8545 independent reflections. The title complex is composed of a discrete [CuL3]2+ cation, uncoordinated ClO4- anions, H2O and CH3CN molecules. The central Cu（II） atom is six-coordinated by six nitrogen donors of three ligands. The coordination geometry of Cu（II） could be considered as an approximately ideal octahedral configuration with little static Jahn-Teller distortion （the longest and shortest Cu-N bonds are 2.102 vs 2.139 with the mean length of 2.122 ）, which is very rare for a six-coordinated Cu（II） complex.

Efficient Cleavage of DNA by the Novel Copper (II) Complex Derived From 2,2'-Pyridil Ligand

Copper (II) complex derived from 2, 2'-pyridil ligand has been, demonstrated to cleave efficiently DNA at micromolar concentrations at room temperature and pH 7.25.

Synthesis and Crystal Structure of the Copper(II)Complex with N,N'-Cyclohexanebis(3- formyl- 5-tert-butyl-salicylaldimine)

A mononuclear copper(II) complex CuL with a Schiff-base L (H2L = N,N(- cyclohexanebis(3-formyl-5-tert-butyl-salicylaldimine)) has been synthesized and characterized. X-ray diffraction analysis at room temperature indicates that the complex (Cu C30H36N2O4, Mr = 552.15) crystallizes in tetrahedral system, space group P41 with a = 13.4685(6), c = 31.326(2) ?, V = 5682.5(5) ?3, Z = 8, Dc = 1.291 g/cm3, F(000) = 4656, μ(MoK() = 0.805 mm-1. The final R and Rw factors are 0.0421 and 0.0815, respectively with 4435 observed reflections. The title complex is a neutral molecule in which the ligand is doubly deprotonated, forming a four-coordinated Cu(II) complex by nitrogen and oxygen donors of the ligand and the coordination geometry of Cu(II) could be considered as an approximately planar configuration. There are two independent molecules, having a transoid arrangement in this structure.

Crystal Structure and Inhibitory Activity against Xanthine Oxidase of Bis(μ_2-chloro)-chloro-(μ-N,N'-(2-pyridylmethylene) Furanmethanoamine Copper(II) Complex

A chloro-bridged dinuclear copper（H） complex with ligand 2-pyridylme-thylene- furfurylamine has been synthesized and characterized by single-crystal X-ray diffraction, and its inhibitory activity on xanthine oxidase （XO） was also evaluated. It crystallizes in the triclinic system, space group P 1 with a = 8.0441（16）, b = 8.5663（17）, c = 10.060（2）A, α = 77.52（3）, β = 72.04（3）, γ = 70.12（3）°, V = 615.3（2）A^3, Z = 1, Dc = 1.731 g/cm^3, F（000） = 322, the final R = 0.0401 and wR = 0.0934 for 1971 observed reflections with I 〉 2σ（I）. X-ray analysis reveals that the Cu（II） cation is five-coordinated by two N atoms of Schiff base and three Cl anions. The C-H…Cl intermolecular and intramolecular hydrogen bonds connect the molecules to form a three-dimensional network. This copper（II） complex shows more potent inhibitory activity against XO with IC50 = 3.48μM than the standard inhibitor allopurinol.

Synthesis and Characterization of Nickel(II) and Copper(II) Complexes with Schiff Base (1R,2R)-(-)-Diaminocyclohexane-N,N'-bis(3-tert-butyl-5-(4'-benzoic acid)-salicylidene)

Two homochiral metallosalen complexes, Ni（salen） （salen = （1R,2R）-（-）-diamino- cyclohexane-N,N′-bis（3-tert-butyl-5-（4′-benzoic acid）-salicylidene） 1 and Cu（salen） 2, have been synthesized and characterized by IR, microanalysis, TGA, powder and single-crystal X-ray crystallography. Both 1 and 2 crystallize in orthorhombic space group P21212 with Z = 4. For 1, a = 12.082（2）, b = 15.447（3）, c = 18.784（4）A^°, V= 3505.7（12）A^°3, Mr = 731.50, Dc = 1.386 g/cm^3, μ = 0.606 mm^-1, F（000） = 1544, the final GOOF = 1.043, R = 0.0496 and wR = 0.1248 for 4791 observed reflections with I 〉 2σ（I）. For 2, a = 12.181（2）, b = 15.501（3）, c = 18.877（4） A^°, V = 3564.3（12）A^°3, Mr = 736.33, Dc = 1.372 g/cm^3, μ = 0.665 mm^-1, F（000） = 1548, the final GOOF = 1.062, R = 0.0575 and wR = 0.1508 for 4562 observed reflections with I 〉 2σ（I）. The crystal structures of 1 and 2 are isostructural with very similar supramolecular structures. An infinite two-dimensional network is generated by hydrogen bonding interactions and intermolecular π…π interactions.

SYNTHESIS AND STRUCTURE OF A 2D SHEET－LIKE COPPER（II） COMPLEX WITH BRIDGING PYRIDINE－4－CARBOXYLATE AND TRANS－OXAMIDATE LIGANDS

A complex polymer [Cu2(oxen)(pic)(H20)]n(Clo4)·2nHH2O, there oxen and pic are N.N' -bis(2aminoethyt )oxamide and Pyridine-4-carboxytate, respectivety, was synthesized. It crystallizes in the monoctinic space group P21/c, with a=10.420(2) A. b=13. 118(2) A, c=14 .863(5) A. β=93.05(2)°, and z=4. The structure of this complex consists of 2D network of copper(II) ions bridged by bis-tridentate oxen in transconformation, and by pyridine-4-carboxylate in which the pyridine ring acts as a monodentate group whereas the carboxylate as a tridentate group and bonds to three different copper centres in a novel fashion. The repeating asymmetric unit is a binuclear copper(II) fragment with one of the copper centre locating in a lengthening octahedrat environment, whereas the other in a distorted tetragonal pyramid geometry.

Elimination of GGTA1,CMAH,β4GalNT2 and CIITA genes in pigs compromises human versus pig xenogeneic immune reactions

Background:Pig organ xenotransplantation is a potential solution for the severe organ shortage in clinic,while immunogenic genes need to be eliminated to improve the immune compatibility between humans and pigs.Current knockout strategies are mainly aimed at the genes causing hyperacute immune rejection(HAR)that occurs in the first few hours while adaptive immune reactions orchestrated by CD4 T cell thereafter also cause graft failure,in which process the MHCⅡmolecule plays critical roles.Methods:Thus,we generate a 4-gene(GGTA1,CMAH,β4GalNT2,and CIITA)knockout pig by CRISPR/Cas9 and somatic cell nuclear transfer to compromise HAR and CD4 T cell reactions simultaneously.Results:We successfully obtained 4KO piglets with deficiency in all alleles of genes,and at cellular and tissue levels.Additionally,the safety of our animals after gene editing was verified by using whole-genome sequencing and karyotyping.Piglets have survived for more than one year in the barrier,and also survived for more than 3 months in the conventional environment,suggesting that the piglets without MHCⅡcan be raised in the barrier and then gradually mated in the conventional environment.Conclusions:4KO piglets have lower immunogenicity,are safe in genomic level,and are easier to breed than the model with both MHCⅠandⅡdeletion.

CRYSTAL STRUCTURE OF THE COPPER（II） COMPLEX OF NEW MACROCYCLIC DIOXOTETRAAMINE LIGAND FUNCTIONALIZED WITH 8－METHYL QUINOLINE PENDANT

The copper (II) complex (1) of a new Iigand 4- (quinolin-8-ylmethyl)- 1, 4, 7. 10-tetraazacycoltridecane-11. 13-dione (L) has been syntehsized and its crystal structure determined. It is of orthorhomic crystal system and Pcad space group with a=16.260 (3), b=7. 739 (2). c=27. 530 (9) A, Mr=416. 97, Z=8. Dx=1. 595 g/cm3,μ=12. 876 cm-1, and F(000)=1736. The final R factor is 0. 061 for 2594 observed reflections with I>3σ(I). The Cu .atom is five coordinated to form a distorted square pyramid geometry in which N(11) of the pendant is at apical site.

DNA Cleavage of the Copper(II) Complexes with a Polyamine Ditopic Ligand

A new binuclear complex [Cu2L(OH)](ClO4)3·2H2O has been synthesized and characterized, where L=2,6-bis{[bis-(2-aminoethyl)amino]methyl}-benzene. In the presence of 0.5 mmol/L complex at pH 8.10 and 37°C, the complex can efficiently cleavage pBR322 DNA with a rate constant kobs of 1.35×10-4 s-1. The cleavage occurred by a non-oxidative mechanism showing activity to be dependent on pH.