A new method was developed for the determination of sodium copper chlorophyll(SCC) by cloud point extraction preconcentration and spectrophotometry, for which Triton X-114 was selected as a nonionic surfactant. Severa...A new method was developed for the determination of sodium copper chlorophyll(SCC) by cloud point extraction preconcentration and spectrophotometry, for which Triton X-114 was selected as a nonionic surfactant. Several factors affecting the extraction efficiency of SCC and its subsequent determination, including the p H of the sample solution, salt and surfactant concentrations, and equilibration temperature and time, were studied and optimized. The extraction efficiency approached 99.4%.The calibration graph under the optimum conditions was linear in the concentration range of 3–220 mg/L with correlation coefficients> 0.9997(n = 8). The limit of detection for the analytes was 0.6 mg/L(S/N = 3). The proposed method is inexpensive, simple, and accurate for the extraction and determination of SCC in food samples.展开更多
The analyical electron microscopy (AEM) was employed to observe the intratissuelar distribution of polysorbate 80 coated nanoparticles in brain with copper chlorophyll (CC).and a safe and cheap pigment was used as...The analyical electron microscopy (AEM) was employed to observe the intratissuelar distribution of polysorbate 80 coated nanoparticles in brain with copper chlorophyll (CC).and a safe and cheap pigment was used as a marker,AEM analyses show that some nanoparticles are located at the wall of the nicrovasculum in brain,while others are distributed around the nicrovasculum in brain ,These results may support that T-80 coated nanoparticles cross the BBB through mechanisms of endocytosis or transcytosis.展开更多
文摘A new method was developed for the determination of sodium copper chlorophyll(SCC) by cloud point extraction preconcentration and spectrophotometry, for which Triton X-114 was selected as a nonionic surfactant. Several factors affecting the extraction efficiency of SCC and its subsequent determination, including the p H of the sample solution, salt and surfactant concentrations, and equilibration temperature and time, were studied and optimized. The extraction efficiency approached 99.4%.The calibration graph under the optimum conditions was linear in the concentration range of 3–220 mg/L with correlation coefficients> 0.9997(n = 8). The limit of detection for the analytes was 0.6 mg/L(S/N = 3). The proposed method is inexpensive, simple, and accurate for the extraction and determination of SCC in food samples.
文摘The analyical electron microscopy (AEM) was employed to observe the intratissuelar distribution of polysorbate 80 coated nanoparticles in brain with copper chlorophyll (CC).and a safe and cheap pigment was used as a marker,AEM analyses show that some nanoparticles are located at the wall of the nicrovasculum in brain,while others are distributed around the nicrovasculum in brain ,These results may support that T-80 coated nanoparticles cross the BBB through mechanisms of endocytosis or transcytosis.