Developing high-efficiency base editors by combining optimized synergistic core components with new types of nuclear localization signal peptide

The clustered regularly interspaced short palindromic repeats(CRISPR)–CRISPR-associated protein(Cas) system has been widely used for genome editing. In this system, the cytosine base editor(CBE) and adenine base editor(ABE) allow generating precise and irreversible base mutations in a programmable manner and have been used in many different types of cells and organisms. However, their applications are limited by low editing efficiency at certain genomic target sites or at specific target cytosine(C) or adenine(A) residues. Using a strategy of combining optimized synergistic core components, we developed a new multiplex super-assembled ABE(sABE) in rice that showed higher base-editing efficiency than previously developed ABEs. We also designed a new type of nuclear localization signal(NLS) comprising a FLAG epitope tag with four copies of a codon-optimized NLS(F4NLS^(r2)) to generate another ABE named F4NLS-sABE. This new NLS increased editing efficiency or edited additional A at several target sites. A new multiplex super-assembled CBE(sCBE) and F4NLS^(r2) involved F4NLS-sCBE were also created using the same strategy. F4NLS-sCBE was proven to be much more efficient than sCBE in rice. These optimized base editors will serve as powerful genome-editing tools for basic research or molecular breeding in rice and will provide a reference for the development of superior editing tools for other plants or animals.

Analysis of Core Technology Problems and Countermeasures in the Robot Industry

Since 2013,China has been the world’s largest market for industrial robots.Despite the gradual maturity of the industrial robot system,the lagging R&D and backward technology level of industrial robots have led to a strong dependence on the import of core components and key technologies,which to a certain extent has restricted the development and improvement of industrial robots.At present,the“neck problem”in the field of industrial robots in China is not only in the reducer,controller,and servo but also in the basic processing equipment,basic technology,and basic materials.In this paper,we propose measures to improve the“neck problem”of industrial robots to promote the high-quality development of industrial robots in China.

Study and Analysis of a Landing Gear Actuating Cylinder Inner-lock

Three kinds of landing gear actuating cylinder inner-locks of an aircrafi ： block ring lock, steel ball lock, finger lock are taken as the study object based on the mechanics, geometry, materials, technology etc and some aircrafi typical inner-lock practical applications. The working principle of the three typical actuating cylinder innerlocks are expounded and the stress and workmanship requirements of the three inner-lock core components are analyzed. The advantages and disadvantages of different kinds are compared and the characteristics and applications of the three inner-locks investigated. The research and analysis results provide valuable information for the actuating cylinder inner-lock of the aircraft landing gear design.