Three-layer core/shell latex particles with various shell crosslinking level and shell thickness were prepared by multistep emulsion polymerization, and the hollow latex particles with different morphologies were then...Three-layer core/shell latex particles with various shell crosslinking level and shell thickness were prepared by multistep emulsion polymerization, and the hollow latex particles with different morphologies were then obtained after alkali post-treatment. Influences of divinyl benzene （DVB） content and the core/shell mass ratio on emulsion polymerization and particle morphology were investigated. Results showed that with the increase of DVB content, the percentage of total amount of --COOH on the particle surface and free in aqueous phase （PSFa） decreased, and the morphology of the post-treated particles underwent evolution from cracked, intact hollow to deficient swelling structure. Decreasing the core/shell mass ratio could not only make more carboxyl groups encapsulated by the shell, but also increase the shell resistance to the swelling of the core. The uniform hollow latex particles with intact morphology were obtained when the DVB content was 3.54 wt% and the core/shell mass ratio was 1/6.展开更多
Affinity core-shell magnetic nanoparticles （MNPs） were prepared for identifying the target proteins of drugs in the cell lysate when used in combination with nano-high-performance liquid chromatography tandem mass s...Affinity core-shell magnetic nanoparticles （MNPs） were prepared for identifying the target proteins of drugs in the cell lysate when used in combination with nano-high-performance liquid chromatography tandem mass spectrometry （HPLC-MS/MS）-based shotgun proteomic analysis. A number of new potential targets of cyclosporine A （CsA） could be identified, owing to the high efficacy of the affinity MNPs in drug target identification. To the best of our knowledge, this is the first time to reveal such an abundant target spectrum of CsA.展开更多
文摘Three-layer core/shell latex particles with various shell crosslinking level and shell thickness were prepared by multistep emulsion polymerization, and the hollow latex particles with different morphologies were then obtained after alkali post-treatment. Influences of divinyl benzene （DVB） content and the core/shell mass ratio on emulsion polymerization and particle morphology were investigated. Results showed that with the increase of DVB content, the percentage of total amount of --COOH on the particle surface and free in aqueous phase （PSFa） decreased, and the morphology of the post-treated particles underwent evolution from cracked, intact hollow to deficient swelling structure. Decreasing the core/shell mass ratio could not only make more carboxyl groups encapsulated by the shell, but also increase the shell resistance to the swelling of the core. The uniform hollow latex particles with intact morphology were obtained when the DVB content was 3.54 wt% and the core/shell mass ratio was 1/6.
基金the National Natural Science Foundations of China，the National Key Laboratory of Organic Biochemistry Opening Foundations
文摘Affinity core-shell magnetic nanoparticles （MNPs） were prepared for identifying the target proteins of drugs in the cell lysate when used in combination with nano-high-performance liquid chromatography tandem mass spectrometry （HPLC-MS/MS）-based shotgun proteomic analysis. A number of new potential targets of cyclosporine A （CsA） could be identified, owing to the high efficacy of the affinity MNPs in drug target identification. To the best of our knowledge, this is the first time to reveal such an abundant target spectrum of CsA.
