A growth experiment was conducted on cobia(Rachycentron canadum,initial weight 108.2 g ± 3.0 g) to investigate the effects of dietary corn gluten meal(CGM) levels on the fish growth,whole body composition and pro...A growth experiment was conducted on cobia(Rachycentron canadum,initial weight 108.2 g ± 3.0 g) to investigate the effects of dietary corn gluten meal(CGM) levels on the fish growth,whole body composition and protein metabolism in relation to specific gene expression.Five isonitrogenous(crude protein 45%) and isoenergetic(gross energy 20 kJ g 1) practical diets were formulated by replacing 0%(the control),17.5%,35.0%,52.5%,and 70.0% of fish meal(FM) protein with CGM protein.No significant differences were observed in the survival,feed intake(FI),specific growth rate(SGR),feed efficiency(FE) and protein productive value(PPV) among fish fed diets with 0%,17.5%,35.0%,and 52.5% of CGM protein.However,these indices were significantly lower in fish fed the diet with 70.0% of CGM protein than those in fish fed the control diet(P < 0.05).The whole-body crude protein and lipid contents were significantly lower while the whole-body moisture content was significantly higher in fish fed the diet with 70.0% of CGM protein compared with the control group(P < 0.05).When 70.0% of FM protein was replaced by CGM,plasma total protein and cholesterol contents were significantly lower than those in the control group(P < 0.05).Fish fed the diet with 70.0% of CGM protein had significantly lower hepatic insulin-like growth factor I(IGF-I) expression levels than those in the control group(P < 0.05).However,no significant differences were observed in hepatic target of rapamycin(TOR),dorsal muscle IGF-I and TOR expression levels among dietary treatments.Results of the present study indicated that 52.5% of FM protein could be replaced by CGM in the diets without significant influences on the growth,feed utilization and protein metabolism of juvenile cobia.The present results might be useful for developing cost effective and sustainable cobia dietary formulations.展开更多
Effects of crop rotation on soybean (Glycine max (L) Merr.) seed composition have not been well investigated. Therefore, the objective of this study was to investigate the effects of soybean-corn (Zea mays L.) rotatio...Effects of crop rotation on soybean (Glycine max (L) Merr.) seed composition have not been well investigated. Therefore, the objective of this study was to investigate the effects of soybean-corn (Zea mays L.) rotations on seed protein, oil, and fatty acids composition on soybean. Soybeans were grown at Stoneville, MS, from 2005 to 2008 in five different scheduled cropping sequences. In 2007, following three years of rotation with corn, seed oleic acid percentage was significantly higher in any crop rotation than continuous soybean. The increase of oleic fatty acid ranged from 61 to 68% in 2007, and from 27 to 51% in 2008, depending on the rotation. The increase of oleic acid was accompanied by significant increases in seed concentrations of phosphorus (P), iron (Fe), and boron (B). In 2007, the increase of P ranged from 60 to 75%, Fe from 70 to 72%, and B from 34 to 69%. In 2008, the increase of P ranged from 82 to 106%, Fe from 32 to 84%, and B from 62 to 77%. Continuous soybean had higher linoleic:oleic ratio and linoleic: palmitic + stearic + oleic ratio, indicating that relative quantity of linoleic acid decreased in rotated crops. The total production of protein, oil, stearic and oleic fatty acids was the lowest in continuous soybean. The total production of palmitic acid was inconsistent across years. The results show that soybean- corn rotation affects seed composition by consistently increasing seed oleic fatty acid, P, Fe, and B concentrations. Higher oleic acid, unsaturated fatty acid, is desirable for oil stability and long-shelf storage. The mechanisms of how these nutrients are involved are not yet understood.展开更多
[Objective] The paper was to study the effects of different levels of NaOH on fiber, crude protein and soluble sugar content of cornstraw. [Method] Four treatments were designed in the test, namely control group, grou...[Objective] The paper was to study the effects of different levels of NaOH on fiber, crude protein and soluble sugar content of cornstraw. [Method] Four treatments were designed in the test, namely control group, group 1, group2 and group 3, which were added with 0, 2%, 3%and 4% NaOH according to the dry weight of corn straw, and the moisture of corn straw after treatment was 45%. After being treated at room tem-perature for 48 h, sensory evaluation and nutrient composition analysis were performed for each group, and the contents of neutral detergent fiber(NDF), acid detergent fiber (ADF), crude protein (CP) and soluble sugar (SS) were measured. [Result] With the increasing addition level of NaOH,the content of NDF and ADF decreased gradually, and significant differences were observed between group 3 (adding 4% NaOH) and control group(P〈0.05). There was no significant difference in CP content among treatment groups(P〉0.05); the SS content gradually increased, and there weresignificant differences between treatment groups and control group (P〈0.05). [Conclusion] 4% NaOH reduced the fiber content and increased thesoluble sugar content of corn straw.展开更多
In order to study the microwave-assisted foam-mat drying properties of corn soaking water and optimize process parameters, a quadratic regression orthogonal rotary method was used to analyze the influence of microwave...In order to study the microwave-assisted foam-mat drying properties of corn soaking water and optimize process parameters, a quadratic regression orthogonal rotary method was used to analyze the influence of microwave power, material weight, material thickness and drying time on moisture content(dry basis), color value and protein content. Results showed that the primary and secondary sequence of parameters with regard to moisture content(d. b.) was drying time, microwave power, material weight and material thickness; the primary and secondary sequence of parameters with regard to color value was material weight, drying time, microwave power and material thickness; the primary and secondary sequence of parameters with regard to protein content was drying time, material weight, microwave power and material thickness. Optimum conditions were obtained as microwave power of 560 W, material weight of 46.88 g, material thickness of 6.20 mm and drying time of 8.01 min. The results might provide the theoretical basis and technical support for the microwave-assisted foam-mat drying of corn soaking water to produce yeast protein power.展开更多
A 9-week feeding trial in floating freshwater cages(1.0 m×1.0 m×2.0 m) was conducted to study the effects of different dietary levels of protein and starch on growth,body composition,and gene expression of e...A 9-week feeding trial in floating freshwater cages(1.0 m×1.0 m×2.0 m) was conducted to study the effects of different dietary levels of protein and starch on growth,body composition,and gene expression of enzymes in common carp,Cyprinus carpio(mean body weight,36.12±1.18 g) to evaluate the protein-sparing effect of dietary carbohydrate.Four diets were formulated with corn starch as the carbohydrate source to obtain corn starch levels of 6.5%,13%,19.5%,or 26%and protein levels of 30.5%,28.2%,26.4%,and 24.2%.The results showed no differences in growth performance of fish fed the diets with different protein and corn starch levels,but body composition and glucose metabolic enzyme activity of carp were significantly affected by the different diets(P<0.05).Weight gain,specific growth rate,and the feed conversion ratio were not different in fish fed the different dietary treatments.Protein efficiency ratio increased significantly as corn starch level increased(P<0.05).Whole-body crude lipid composition increased with increasing dietary corn starch level(P<0.05).Glucokinase(GK),hexokinase,and pyruvate kinase(PK) activities increased significantly with increasing dietary corn starch level(P<0.05),whereas glucose-6-phosphate(G6Pase) activity decreased with increasing dietary corn starch level(P<0.05).GK gene expression was significantly higher in fish fed the high-corn starch diet than those fed the low-corn starch diet(P<0.05).G6 pase gene expression tended to decrease with increasing starch level(P>0.05).In summary,the results indicate a protein-sparing effect by substituting carbohydrate in the diet of common carp.展开更多
Biochemical technique was used to separate three kinds of proteins (albumin, globulin and gliadin) in corn samples froin high selenium areas and normal areas in Erxi autonomous region of Hubei Province, China. The con...Biochemical technique was used to separate three kinds of proteins (albumin, globulin and gliadin) in corn samples froin high selenium areas and normal areas in Erxi autonomous region of Hubei Province, China. The contents of Se and other elements in these proteins were determined by neutron activation analysis (NAA). The results show that Se is enriched in corn proteins at high selenium area, while Cu, Al, Mn, V and Cl are also enriched in varying degrees.展开更多
An improved population NEAU 2 was tested in Triple Test Cross design to analyze the genetic structure of 31 plants and quality traits. The results suggested that additive effect played an important role in all of thes...An improved population NEAU 2 was tested in Triple Test Cross design to analyze the genetic structure of 31 plants and quality traits. The results suggested that additive effect played an important role in all of these traits except for filling property trait V1. Nine traits such as grain yield and volume weight were determined mainly by the additive effect. Globulin and starch content were controlled by significant dominant effect. The leaf number above ear and most quality traits studied were controlled by both additive and dominance effect, and 4 traits such as 100 kernel weight were determined by both additive and epistasis effect. However, the heredity of plant height, ear position, and so on was determined by all these kinds of genetic effects. In quality improvement, direct selection could increase the content but not improve the quality of corn protein. Direct selection for albumin content and the ratio of glutelin to zein was effective and might be a better way to improve the protein quality of corn. Starch content might be raised by direct selection, but it might increase the content of amylopectin meanwhile. It was better to increase gross fat content by direct and indirect selection through the weight ratio of embryo to endosperm, which might also be effective to increase gross fat.展开更多
基金the National Department Public Benefit Research Foundation(Ministry of Agriculture of the People’s Republic of China,No.201003020)the National Natural Science Foundation of China(No.30901108)Agricultural Scientific and Technological Achievements into Capital 2010GB23600673
文摘A growth experiment was conducted on cobia(Rachycentron canadum,initial weight 108.2 g ± 3.0 g) to investigate the effects of dietary corn gluten meal(CGM) levels on the fish growth,whole body composition and protein metabolism in relation to specific gene expression.Five isonitrogenous(crude protein 45%) and isoenergetic(gross energy 20 kJ g 1) practical diets were formulated by replacing 0%(the control),17.5%,35.0%,52.5%,and 70.0% of fish meal(FM) protein with CGM protein.No significant differences were observed in the survival,feed intake(FI),specific growth rate(SGR),feed efficiency(FE) and protein productive value(PPV) among fish fed diets with 0%,17.5%,35.0%,and 52.5% of CGM protein.However,these indices were significantly lower in fish fed the diet with 70.0% of CGM protein than those in fish fed the control diet(P < 0.05).The whole-body crude protein and lipid contents were significantly lower while the whole-body moisture content was significantly higher in fish fed the diet with 70.0% of CGM protein compared with the control group(P < 0.05).When 70.0% of FM protein was replaced by CGM,plasma total protein and cholesterol contents were significantly lower than those in the control group(P < 0.05).Fish fed the diet with 70.0% of CGM protein had significantly lower hepatic insulin-like growth factor I(IGF-I) expression levels than those in the control group(P < 0.05).However,no significant differences were observed in hepatic target of rapamycin(TOR),dorsal muscle IGF-I and TOR expression levels among dietary treatments.Results of the present study indicated that 52.5% of FM protein could be replaced by CGM in the diets without significant influences on the growth,feed utilization and protein metabolism of juvenile cobia.The present results might be useful for developing cost effective and sustainable cobia dietary formulations.
文摘Effects of crop rotation on soybean (Glycine max (L) Merr.) seed composition have not been well investigated. Therefore, the objective of this study was to investigate the effects of soybean-corn (Zea mays L.) rotations on seed protein, oil, and fatty acids composition on soybean. Soybeans were grown at Stoneville, MS, from 2005 to 2008 in five different scheduled cropping sequences. In 2007, following three years of rotation with corn, seed oleic acid percentage was significantly higher in any crop rotation than continuous soybean. The increase of oleic fatty acid ranged from 61 to 68% in 2007, and from 27 to 51% in 2008, depending on the rotation. The increase of oleic acid was accompanied by significant increases in seed concentrations of phosphorus (P), iron (Fe), and boron (B). In 2007, the increase of P ranged from 60 to 75%, Fe from 70 to 72%, and B from 34 to 69%. In 2008, the increase of P ranged from 82 to 106%, Fe from 32 to 84%, and B from 62 to 77%. Continuous soybean had higher linoleic:oleic ratio and linoleic: palmitic + stearic + oleic ratio, indicating that relative quantity of linoleic acid decreased in rotated crops. The total production of protein, oil, stearic and oleic fatty acids was the lowest in continuous soybean. The total production of palmitic acid was inconsistent across years. The results show that soybean- corn rotation affects seed composition by consistently increasing seed oleic fatty acid, P, Fe, and B concentrations. Higher oleic acid, unsaturated fatty acid, is desirable for oil stability and long-shelf storage. The mechanisms of how these nutrients are involved are not yet understood.
基金Supported by PhD Start-up Fund of Inner Mongolia University for Nationalities(BS291)
文摘[Objective] The paper was to study the effects of different levels of NaOH on fiber, crude protein and soluble sugar content of cornstraw. [Method] Four treatments were designed in the test, namely control group, group 1, group2 and group 3, which were added with 0, 2%, 3%and 4% NaOH according to the dry weight of corn straw, and the moisture of corn straw after treatment was 45%. After being treated at room tem-perature for 48 h, sensory evaluation and nutrient composition analysis were performed for each group, and the contents of neutral detergent fiber(NDF), acid detergent fiber (ADF), crude protein (CP) and soluble sugar (SS) were measured. [Result] With the increasing addition level of NaOH,the content of NDF and ADF decreased gradually, and significant differences were observed between group 3 (adding 4% NaOH) and control group(P〈0.05). There was no significant difference in CP content among treatment groups(P〉0.05); the SS content gradually increased, and there weresignificant differences between treatment groups and control group (P〈0.05). [Conclusion] 4% NaOH reduced the fiber content and increased thesoluble sugar content of corn straw.
文摘In order to study the microwave-assisted foam-mat drying properties of corn soaking water and optimize process parameters, a quadratic regression orthogonal rotary method was used to analyze the influence of microwave power, material weight, material thickness and drying time on moisture content(dry basis), color value and protein content. Results showed that the primary and secondary sequence of parameters with regard to moisture content(d. b.) was drying time, microwave power, material weight and material thickness; the primary and secondary sequence of parameters with regard to color value was material weight, drying time, microwave power and material thickness; the primary and secondary sequence of parameters with regard to protein content was drying time, material weight, microwave power and material thickness. Optimum conditions were obtained as microwave power of 560 W, material weight of 46.88 g, material thickness of 6.20 mm and drying time of 8.01 min. The results might provide the theoretical basis and technical support for the microwave-assisted foam-mat drying of corn soaking water to produce yeast protein power.
基金Supported by the Key Technology R&D Program of Tianjin(No.13ZCZDNC00900)the Tianjin Research Program of Application Foundation and Advanced Technology(No.14JCQNJC15100)+2 种基金the Tianjin Innovative Research Team(No.TD12-5018)the Tianjin Key Laboratory of Animal and Plant Resistancethe National Natural Science Foundation of China(No.31402313)
文摘A 9-week feeding trial in floating freshwater cages(1.0 m×1.0 m×2.0 m) was conducted to study the effects of different dietary levels of protein and starch on growth,body composition,and gene expression of enzymes in common carp,Cyprinus carpio(mean body weight,36.12±1.18 g) to evaluate the protein-sparing effect of dietary carbohydrate.Four diets were formulated with corn starch as the carbohydrate source to obtain corn starch levels of 6.5%,13%,19.5%,or 26%and protein levels of 30.5%,28.2%,26.4%,and 24.2%.The results showed no differences in growth performance of fish fed the diets with different protein and corn starch levels,but body composition and glucose metabolic enzyme activity of carp were significantly affected by the different diets(P<0.05).Weight gain,specific growth rate,and the feed conversion ratio were not different in fish fed the different dietary treatments.Protein efficiency ratio increased significantly as corn starch level increased(P<0.05).Whole-body crude lipid composition increased with increasing dietary corn starch level(P<0.05).Glucokinase(GK),hexokinase,and pyruvate kinase(PK) activities increased significantly with increasing dietary corn starch level(P<0.05),whereas glucose-6-phosphate(G6Pase) activity decreased with increasing dietary corn starch level(P<0.05).GK gene expression was significantly higher in fish fed the high-corn starch diet than those fed the low-corn starch diet(P<0.05).G6 pase gene expression tended to decrease with increasing starch level(P>0.05).In summary,the results indicate a protein-sparing effect by substituting carbohydrate in the diet of common carp.
文摘Biochemical technique was used to separate three kinds of proteins (albumin, globulin and gliadin) in corn samples froin high selenium areas and normal areas in Erxi autonomous region of Hubei Province, China. The contents of Se and other elements in these proteins were determined by neutron activation analysis (NAA). The results show that Se is enriched in corn proteins at high selenium area, while Cu, Al, Mn, V and Cl are also enriched in varying degrees.
基金Science Project of Heilongjiang Province (GB01B102- 02- 05)
文摘An improved population NEAU 2 was tested in Triple Test Cross design to analyze the genetic structure of 31 plants and quality traits. The results suggested that additive effect played an important role in all of these traits except for filling property trait V1. Nine traits such as grain yield and volume weight were determined mainly by the additive effect. Globulin and starch content were controlled by significant dominant effect. The leaf number above ear and most quality traits studied were controlled by both additive and dominance effect, and 4 traits such as 100 kernel weight were determined by both additive and epistasis effect. However, the heredity of plant height, ear position, and so on was determined by all these kinds of genetic effects. In quality improvement, direct selection could increase the content but not improve the quality of corn protein. Direct selection for albumin content and the ratio of glutelin to zein was effective and might be a better way to improve the protein quality of corn. Starch content might be raised by direct selection, but it might increase the content of amylopectin meanwhile. It was better to increase gross fat content by direct and indirect selection through the weight ratio of embryo to endosperm, which might also be effective to increase gross fat.