Background:Green tea,mulberry leaf and corn silk are traditional herbs used in the prevention and treatment of diabetes in China for a long time,but their synergistic hypoglycemic effects and mechanisms remain unclear...Background:Green tea,mulberry leaf and corn silk are traditional herbs used in the prevention and treatment of diabetes in China for a long time,but their synergistic hypoglycemic effects and mechanisms remain unclear.Methods:The effective components of green tea,mulberry leaf and corn silk were extracted and enriched.Mixture design of experiments was used to study the influences of different combinations on the cell viability and glucose uptake level of L6 myoblasts,so as to determine the optimal synergistic hypoglycemic combination.The possible hypoglycemic mechanism of the optimal synergistic combination was explored by cytotoxicity assay,glucose uptake assay,and western blot.Results:Three polyphenol enrichment fractions of the herbs,30%ethanol elution fraction of green tea(GT),50%ethanol elution fraction of mulberry leaf(ML)and 60%ethanol elution fraction of corn silk(CS)were obtained.The antioxidant activities of GT-30%,ML-50%and CS-60%were superior to those of crude extracts,and showed strong potential inα-amylase andα-glucosidase inhibition activities.The optimal synergistic combination of crude extracts G7(crude extract of green tea:crude extract of mulberry leaf:crude extract of corn silk=1:5:3),polyphenol enrichment fractions R3(GT-30%:ML-50%:CS-60%=1:7:1)and monomers X2(epigallocatechin gallate:morusin:formononetin=3:1:2)were selected,respectively.G7,R3,and X2 showed promoting effects on the cell viability and glucose uptake of L6 myoblasts within the detected concentration range.In addition,G7,R3,and X2 could increase the expression levels of p-PI3K/PI3K and p-Akt/Akt in L6 myoblasts,and promote the translocation of Glut4,but G7 and R3 showed more significant effects.Conclusion:The synergistic hypoglycemic effects of green tea,mulberry leaf and corn silk had the characteristics of multiple-components and multiple-targets with p-PI3K/PI3K,p-Akt/Akt and the translocation of Glut4 signal pathways involved.The three traditional herbs might have the potential to be combined used for the prevention and treatment of diabetes based on the synergistic hypoglycemic effects.展开更多
This study was carried out to evaluate the changes in silk activity of two corn cultivars Xundan 518 and Yongyou 618 after silk emergence. Artificial pollination was conducted at different days after silk emergence, a...This study was carried out to evaluate the changes in silk activity of two corn cultivars Xundan 518 and Yongyou 618 after silk emergence. Artificial pollination was conducted at different days after silk emergence, and the ear length, ear diameter,barren tip length, 100-grain weight, grain number per ear and grain weight per ear were measured at harvest. The results showed that with the delay of pollination, the number and weight of grains per ear increased at first, peaked at D2 to D4, and decreased subsequently; ear diameter and 100-grain weight showed a downward trend. Correlation analysis revealed that grain number per ear and grain weight per ear were most closely correlated. Rapid silk extrusion and long-lasting silk activity proved that the two corn cultivars Xundan 518 and Yongyou 618 are tolerant to high temperature.展开更多
For the value-added utilization of discarded agricultural wastes,corn silk( CS) obtained abundantly in the farming field has been tested as a new source of cellulosic materials. Cellulose microfibril( CMF) and cellulo...For the value-added utilization of discarded agricultural wastes,corn silk( CS) obtained abundantly in the farming field has been tested as a new source of cellulosic materials. Cellulose microfibril( CMF) and cellulose nanocrystal( CNC) were isolated from CS by ethanol and alkali pretreatments,and acid hydrolysis.The characterization was performed by scanning electron microscopy( SEM),Fourier transform infrared spectroscopy( FT-IR),X-ray diffraction( XRD), thermogravimetric analysis( TGA) and transmission electron microscopy( TEM). After chemical pretreatments,the lignin,hemicelluloses and other non-structural components were removed. The degree of crystallinity and thermal stability of CMF and CNC were increased compared to raw CS. The crystallinity indexes of CS,CMF and CNC were 45. 90%,65. 77%,and 73. 75% respectively. The CNC was flat and rod like shape with diameter and aspect ratio range of 13. 96-33. 69 nm and 34. 34-23. 02 nm respectively. The nanocrystals had an alternative potential to be used as reinforcing filler for bio-nanocomposites preparation.展开更多
The aim of this study was to use dried corn silk powder variety of VL Baby corn-1 for formulation of flavoured herbal tea with different combination of Thyme(Trachyspermum ammi),Clove(Syzygium aromaticum),Cinnamon pow...The aim of this study was to use dried corn silk powder variety of VL Baby corn-1 for formulation of flavoured herbal tea with different combination of Thyme(Trachyspermum ammi),Clove(Syzygium aromaticum),Cinnamon powder(Cinnamomum verum),Timur(Zanthoxylum alatum),Tulsi(Holy basil)(Ocimum tenuiflorum),Gandraini(Achillea millefolium),Lemon grass(Cymbopogon citratus),Blend(Clove+Timur+Gandraini),and one only corn silk without any other herbs and to know antioxidant properties of developed tea.Result of sensory evaluation experiment showed that all combinations were liked moderately.Lemon grass flavour followed by cinnamon and clove blend obtained highest score.Total phenolic content,total flavonoid content,DPPH free radical scavenging activity and total antioxidant activity for all flavour are analyzed and reported.Results on DPPH free radical scavenging activity indicated that corn silk tea had 81.215±0.75μg/mL whereas blend with Timur had significant highest value and Thyme blend had lowest value 64.20±0.40 among all eight different blends with corn silk.The values for total antioxidant capacity blends with Timur(Zanthoxylum alatu)in corn silk showed significantly(P<0.05)highest value and Clove,Timur,Gandraini blends got lowest value.展开更多
基金the grant from National Key Research and Development Program of China(Grant No.2021YFE0110000)the grant from Tianjin Municipal Science and Technology Foundation(Grant No.22JCYBJC00160).
文摘Background:Green tea,mulberry leaf and corn silk are traditional herbs used in the prevention and treatment of diabetes in China for a long time,but their synergistic hypoglycemic effects and mechanisms remain unclear.Methods:The effective components of green tea,mulberry leaf and corn silk were extracted and enriched.Mixture design of experiments was used to study the influences of different combinations on the cell viability and glucose uptake level of L6 myoblasts,so as to determine the optimal synergistic hypoglycemic combination.The possible hypoglycemic mechanism of the optimal synergistic combination was explored by cytotoxicity assay,glucose uptake assay,and western blot.Results:Three polyphenol enrichment fractions of the herbs,30%ethanol elution fraction of green tea(GT),50%ethanol elution fraction of mulberry leaf(ML)and 60%ethanol elution fraction of corn silk(CS)were obtained.The antioxidant activities of GT-30%,ML-50%and CS-60%were superior to those of crude extracts,and showed strong potential inα-amylase andα-glucosidase inhibition activities.The optimal synergistic combination of crude extracts G7(crude extract of green tea:crude extract of mulberry leaf:crude extract of corn silk=1:5:3),polyphenol enrichment fractions R3(GT-30%:ML-50%:CS-60%=1:7:1)and monomers X2(epigallocatechin gallate:morusin:formononetin=3:1:2)were selected,respectively.G7,R3,and X2 showed promoting effects on the cell viability and glucose uptake of L6 myoblasts within the detected concentration range.In addition,G7,R3,and X2 could increase the expression levels of p-PI3K/PI3K and p-Akt/Akt in L6 myoblasts,and promote the translocation of Glut4,but G7 and R3 showed more significant effects.Conclusion:The synergistic hypoglycemic effects of green tea,mulberry leaf and corn silk had the characteristics of multiple-components and multiple-targets with p-PI3K/PI3K,p-Akt/Akt and the translocation of Glut4 signal pathways involved.The three traditional herbs might have the potential to be combined used for the prevention and treatment of diabetes based on the synergistic hypoglycemic effects.
基金Supported by Major Science and Technology Project of Henan Province(161100110500-0104)Special Fund for Meteorologicalscientific Research in the Public Interest(GYHY201406026)
文摘This study was carried out to evaluate the changes in silk activity of two corn cultivars Xundan 518 and Yongyou 618 after silk emergence. Artificial pollination was conducted at different days after silk emergence, and the ear length, ear diameter,barren tip length, 100-grain weight, grain number per ear and grain weight per ear were measured at harvest. The results showed that with the delay of pollination, the number and weight of grains per ear increased at first, peaked at D2 to D4, and decreased subsequently; ear diameter and 100-grain weight showed a downward trend. Correlation analysis revealed that grain number per ear and grain weight per ear were most closely correlated. Rapid silk extrusion and long-lasting silk activity proved that the two corn cultivars Xundan 518 and Yongyou 618 are tolerant to high temperature.
基金Fundamental Research Funds for the Central Universities,China(Nos.16D310102,2232018A3-04)
文摘For the value-added utilization of discarded agricultural wastes,corn silk( CS) obtained abundantly in the farming field has been tested as a new source of cellulosic materials. Cellulose microfibril( CMF) and cellulose nanocrystal( CNC) were isolated from CS by ethanol and alkali pretreatments,and acid hydrolysis.The characterization was performed by scanning electron microscopy( SEM),Fourier transform infrared spectroscopy( FT-IR),X-ray diffraction( XRD), thermogravimetric analysis( TGA) and transmission electron microscopy( TEM). After chemical pretreatments,the lignin,hemicelluloses and other non-structural components were removed. The degree of crystallinity and thermal stability of CMF and CNC were increased compared to raw CS. The crystallinity indexes of CS,CMF and CNC were 45. 90%,65. 77%,and 73. 75% respectively. The CNC was flat and rod like shape with diameter and aspect ratio range of 13. 96-33. 69 nm and 34. 34-23. 02 nm respectively. The nanocrystals had an alternative potential to be used as reinforcing filler for bio-nanocomposites preparation.
文摘The aim of this study was to use dried corn silk powder variety of VL Baby corn-1 for formulation of flavoured herbal tea with different combination of Thyme(Trachyspermum ammi),Clove(Syzygium aromaticum),Cinnamon powder(Cinnamomum verum),Timur(Zanthoxylum alatum),Tulsi(Holy basil)(Ocimum tenuiflorum),Gandraini(Achillea millefolium),Lemon grass(Cymbopogon citratus),Blend(Clove+Timur+Gandraini),and one only corn silk without any other herbs and to know antioxidant properties of developed tea.Result of sensory evaluation experiment showed that all combinations were liked moderately.Lemon grass flavour followed by cinnamon and clove blend obtained highest score.Total phenolic content,total flavonoid content,DPPH free radical scavenging activity and total antioxidant activity for all flavour are analyzed and reported.Results on DPPH free radical scavenging activity indicated that corn silk tea had 81.215±0.75μg/mL whereas blend with Timur had significant highest value and Thyme blend had lowest value 64.20±0.40 among all eight different blends with corn silk.The values for total antioxidant capacity blends with Timur(Zanthoxylum alatu)in corn silk showed significantly(P<0.05)highest value and Clove,Timur,Gandraini blends got lowest value.
