AIM To study the angiogenesis induced by liver cancer with different metastatic potentials using corneal micropocket model in nude mice.METHODS Corneal micropockets were created in nude mice. Tumor tissues and liver t...AIM To study the angiogenesis induced by liver cancer with different metastatic potentials using corneal micropocket model in nude mice.METHODS Corneal micropockets were created in nude mice. Tumor tissues and liver tissues were implanted into the corneal micropockets. Angiogenesis was observed using a digital camera under slit-lamp biomicroscope, and compared among different grafts and incision alone. Vascular responses were recorded in regard to the range, number and length of new blood vessels toward the grafts or incisions.RESULTS Vascular responses induced by tumor tissues were greater than those by incision alone and liver tissue grafts. LCI-D20 induced more intensive angiogenesis than LCI-D35.CONCLUSION Highly metastatic liver cancer LCI D20 was more angiogenic than low metastatic cancer LCI D35 and liver tissue. Micropocket was a useful model to study dynamic process of angiogenesis in vivo.展开更多
AIM: To choose appropriate concentration of sodium hydroxide(Na OH) solution to establish a stable and consistent corneal alkali burn mouse model in grade II.·METHODS: The mice(n =60) were randomly divided ...AIM: To choose appropriate concentration of sodium hydroxide(Na OH) solution to establish a stable and consistent corneal alkali burn mouse model in grade II.·METHODS: The mice(n =60) were randomly divided into four groups and 15 mice each group. Corneal alkali burns were induced by placing circle filter paper soaked with Na OH solutions on the right central cornea for 30 s.The concentrations of Na OH solutions of groups A, B, C,and D were 0.1 mol/L, 0.15 mol/L, 0.2 mol/L, and 1.0 mol/L respectively. Then these corneas were irrigated with 20 m L physiological saline(0.9% Na Cl). On day 7 postburn, slit lamp microscope was used to observe corneal opacity,corneal epithelial sodium fluorescein staining positive rate, incidence of corneal ulcer and corneal neovascularization, meanwhile pictures of the anterior eyes were taken. Cirrus spectral domain optical coherence tomography was used to scan cornea to observe corneal epithelial defect and corneal ulcer.·RESULTS: Corneal opacity scores( x ±s) were not significantly different between the group A and group B(P =0.097). Incidence of corneal ulcer in group B was significantly higher than that in group A(P =0.035).Incidence of corneal ulcer and perforation rate in group B was lower than that in group C. Groups C and D had corneal neovascularization, and incidence of corneal neovascularization in group D was significantly higher than that in group C(P =0.000).·CONCLUSION: Using 0.15 mol/L Na OH can establish grade II mouse model of corneal alkali burns.展开更多
Corneal blindness caused by limbal stem cell deficiency(LSCD) is one of the most common debilitating eye disorders. Thus far, the most effective treatment for LSCD is corneal transplantation, which is often hindered b...Corneal blindness caused by limbal stem cell deficiency(LSCD) is one of the most common debilitating eye disorders. Thus far, the most effective treatment for LSCD is corneal transplantation, which is often hindered by the shortage of donors. Pluripotent stem cell technology including embryonic stem cells(ESCs) and induced pluripotent stem cells(iPSCs) have opened new avenues for treating this disease. iPSCs-derived corneal epithelial cells provide an autologous and unlimited source of cells for the treatment of LSCD. On the other hand, iPSCs of LSCD patients can be used for iPSCs-corneal disease model and new drug discovery. However, prior to clinical trial, the efficacy and safety of these cells in patients with LSCD should be proved. Here we focused on the current status of iPSCs-derived corneal epithelial cells used for cell therapy as well as for corneal disease modeling. The challenges and potential of iPSCs-derived corneal epithelial cells as a choice for clinical treatment in corneal disease were also discussed.展开更多
基金Supported by the Fund for Leading Specialty of Shanghai Metropolitan Bureau of Public Health
文摘AIM To study the angiogenesis induced by liver cancer with different metastatic potentials using corneal micropocket model in nude mice.METHODS Corneal micropockets were created in nude mice. Tumor tissues and liver tissues were implanted into the corneal micropockets. Angiogenesis was observed using a digital camera under slit-lamp biomicroscope, and compared among different grafts and incision alone. Vascular responses were recorded in regard to the range, number and length of new blood vessels toward the grafts or incisions.RESULTS Vascular responses induced by tumor tissues were greater than those by incision alone and liver tissue grafts. LCI-D20 induced more intensive angiogenesis than LCI-D35.CONCLUSION Highly metastatic liver cancer LCI D20 was more angiogenic than low metastatic cancer LCI D35 and liver tissue. Micropocket was a useful model to study dynamic process of angiogenesis in vivo.
基金Supported by National Science and Technology Major Project of China(No.2011ZXJ09104-10C)
文摘AIM: To choose appropriate concentration of sodium hydroxide(Na OH) solution to establish a stable and consistent corneal alkali burn mouse model in grade II.·METHODS: The mice(n =60) were randomly divided into four groups and 15 mice each group. Corneal alkali burns were induced by placing circle filter paper soaked with Na OH solutions on the right central cornea for 30 s.The concentrations of Na OH solutions of groups A, B, C,and D were 0.1 mol/L, 0.15 mol/L, 0.2 mol/L, and 1.0 mol/L respectively. Then these corneas were irrigated with 20 m L physiological saline(0.9% Na Cl). On day 7 postburn, slit lamp microscope was used to observe corneal opacity,corneal epithelial sodium fluorescein staining positive rate, incidence of corneal ulcer and corneal neovascularization, meanwhile pictures of the anterior eyes were taken. Cirrus spectral domain optical coherence tomography was used to scan cornea to observe corneal epithelial defect and corneal ulcer.·RESULTS: Corneal opacity scores( x ±s) were not significantly different between the group A and group B(P =0.097). Incidence of corneal ulcer in group B was significantly higher than that in group A(P =0.035).Incidence of corneal ulcer and perforation rate in group B was lower than that in group C. Groups C and D had corneal neovascularization, and incidence of corneal neovascularization in group D was significantly higher than that in group C(P =0.000).·CONCLUSION: Using 0.15 mol/L Na OH can establish grade II mouse model of corneal alkali burns.
文摘Corneal blindness caused by limbal stem cell deficiency(LSCD) is one of the most common debilitating eye disorders. Thus far, the most effective treatment for LSCD is corneal transplantation, which is often hindered by the shortage of donors. Pluripotent stem cell technology including embryonic stem cells(ESCs) and induced pluripotent stem cells(iPSCs) have opened new avenues for treating this disease. iPSCs-derived corneal epithelial cells provide an autologous and unlimited source of cells for the treatment of LSCD. On the other hand, iPSCs of LSCD patients can be used for iPSCs-corneal disease model and new drug discovery. However, prior to clinical trial, the efficacy and safety of these cells in patients with LSCD should be proved. Here we focused on the current status of iPSCs-derived corneal epithelial cells used for cell therapy as well as for corneal disease modeling. The challenges and potential of iPSCs-derived corneal epithelial cells as a choice for clinical treatment in corneal disease were also discussed.