Correlation Study on Expression of GST-π Protein in Brain Tissue and Peripheral Blood of Epilepsy Rats Induced by Pilocarpine

Previous studies have suggested that glutathione-S-transferase π （GST-π） over-expression in the brain tissue is associated with refractory epilepsy. However, whether the change in GST-π level in the peripheral blood is in line with that in brain tissue remains unknown. This study examined the correlation between GST-π in brain tissue and that in peripheral blood in rat models of pilocarpine-induced refractory epilepsy. The animals were divided into drug-resistant group and drug-responsive group according to the response to anti-epileptic drugs. GST-π expression in brain tissue was immunohistochemically determined, while the expression of GST-π in peripheral blood was analyzed by Western blotting. In the hippocampus and cortex, GST-π was mainly found in the cytoplasm and membrane of neurons, and the GST-π expression level was higher in drug-resistant group than in the drug-responsive group and saline control group （P〈0.05）. Moreover, there was no significant difference between responders and saline control animals （P〉0.05）. The change in expression of GST-π in peripheral blood showed the same pattern as that in brain tissues, suggesting GST-π might contribute to drug resistance in epilepsy. Importantly, the GST-π over-expression in peripheral blood could be used as a marker for resistance to anti-epileptic agents.

A correlation study between ITGA6 gene,chromosome 8q24,MSMB genes and prostate cancer

Objective To explore the correlation between IT-GA6 gene ( rs12621278, G ) , MSMB gene ( rs10993994,T) ,chromosome 8q24 9 ( rs10086908, T) and prostate cancer ( PCa) in Beijing residents,and to explore the correlation between genotype and pheno-

Correlation between pre-anesthesia anxiety and emergence agitation in non-small cell lung cancer surgery patients

BACKGROUND Preoperative anxiety is a common emotional problem during the perioperative period and may adversely affect postoperative recovery.Emergence agitation(EA)is a common complication of general anesthesia that may increase patient discomfort and hospital stay and may be associated with the development of postoperative complications.Pre-anesthetic anxiety may be associated with the development of EA,but studies in this area are lacking.AIM To determine the relationship between pre-anesthetic anxiety and EA after radical surgery in patients with non-small cell lung cancer(NSCLC).METHODS Eighty patients with NSCLC undergoing surgical treatment between June 2020 and June 2023 were conveniently sampled.We used the Hospital Anxiety and Depression Scale’s(HADS)anxiety subscale(HADS-A)to determine patients’anxiety at four time points(T1-T4):Patients’preoperative visit,waiting period in the surgical waiting room,after entering the operating room,and before anesthesia induction,respectively.The Riker Sedation-Agitation Scale(RSAS)examined EA after surgery.Scatter plots of HADS-A and RSAS scores assessed the correlation between patients’pre-anesthesia anxiety status and EA.We performed a partial correlation analysis of HADS-A scores with RSAS scores.RESULTS NSCLC patients’HADS-A scores gradually increased at the four time points:7.33±2.03 at T1,7.99±2.22 at T2,8.05±2.81 at T3,and 8.36±4.17 at T4.The patients’postoperative RSAS score was 4.49±1.18,and 27 patients scored≥5,indicating that 33.75%patients had EA.HADS-A scores at T3 and T4 were significantly higher in patients with EA(9.67±3.02 vs 7.23±2.31,12.56±4.10 vs 6.23±2.05,P<0.001).Scatter plots showed the highest correlation between HADS-A and RSAS scores at T3 and T4.Partial correlation analysis showed a strong positive correlation between HADS-A and RSAS scores at T3 and T4(r=0.296,0.314,P<0.01).CONCLUSION Agitation during anesthesia recovery in patients undergoing radical resection for NSCLC correlated with anxiety at the time of entering the operating room and before anesthesia induction.

Study on the correlation between TCM syndrome differentiation and immune inflammation index of Sjogren's syndrome

Objective:To observe the relationship between the distribution of TCM Syndromes of primary Sjogren's syndrome(pSS)and the changes of erythrocyte sedimentation rate(ESR),C-reactive protein(CRP),tumor necrosis factor-α(TNF-α),interleukin-6(IL-6),immunoglobulin IgA,IgG and IgM.Methods:157 diagnosed PSS patients were divided into dampness heat obstructing collaterals syndrome,dryness evil invading lung syndrome,Qi blood stasis syndrome,liver kidney yin deficiency syndrome and Qi Yin deficiency syndrome according to TCM syndrome differentiation standard.The age,course of disease,general health score in international universal quality of life scale,self rating Anxiety scale(SAS),self rating Depression Scale(SDS)and erythrocyte sedimentation rate were observed and detected(ESR,C-reactive protein(CRP),tumor necrosis factor-α(TNF-α),interleukin-6(IL-6),immunoglobulin IgA,IgG and IgM and other immune inflammatory indexes were detected.The relationship between different TCM Syndromes of PSS and molecular level changes of immune inflammatory indexes was analyzed and compared,as well as the impact on quality of life and mental emotion.Results:The overall health score of patients with Qi Yin deficiency syndrome was significantly lower than that of patients with dampness heat obstructing collaterals and dampness evil invading lung syndrome(P<0.01),while the SAS and SDS scores of patients with Qi Yin deficiency syndrome were significantly higher than those of patients with dampness heat invading lung syndrome and dampness heat obstructing collaterals syndrome(P<0.05,P<0.01);ESR,CRP,RF,TNF-α,IL-6,IgA,IgG and IgM related laboratory indexes of patients with dampness heat obstructing collaterals syndrome were significantly higher than those of patients with dry evil invading lung syndrome,liver kidney yin deficiency syndrome and liver kidney yin deficiency syndrome Qi Yin deficiency syndrome and Qi blood stasis syndrome(P<0.05,P<0.01).Correlation test found that the above five syndrome types were positively correlated with hs-CRP,IL-6,TNF-ɑindicators(P<0.05).Conclusion:The overall health,SAS,SDS and related hs-CRP,IL-6,TNF-ɑand some others indexes of SS patients are associated with TCM syndromes.

Study on the correlation between bandage bandage and pressure therapy of lymphedema

To explore the effects of bandage technology and pressure therapy in breast cancer with lymphedema.Methods A total of 91 patients,with breast cancer-related lymphedema and hospitalized in a third-class hospital in Jiangsu Province from May to December,2018,were selected and sequenced in class“ABC”in the order of entry.The patients in class“A”were enrolled in“8”shaped bandaging group.The patients in class“B”were enrolled in“5”spiral bandaging group.The patients in class“C”were enrolled in combined bandage group.The primary outcome was the degree of limb swelling used by multiple-frequency bioelectrical impedance analysis.Secondary outcomes were the amount of bandage losses,counting 24-hour bandage loose occurrences and patients satisfaction.All outcomes were evaluated both before and after treatment.Results After treatment,there was a significant difference in the regression of limb swelling in all three groups(P<0.05).However,the largest amplitude of regression was observed in combined bandage group(limb circumference ratio:53%and tissue water ratio:68%).Although“8”bandaging group had large material,bandage cost and minimum comfort,the bandage has good stability and no case of 24-hour bandage loose occurrences was found in this group.The“5”partial binding group had an advantage in the amount of bandage losses and comfort level,but among which 3 patients had the phenomenon of loose bandage at the joint.The combined bandage group had the best therapeutic effect in limb swelling and patients satisfaction(P<0.05).Conclusion The combined bandage group significantly decreased the degree of limb swelling and 24-hour bandage loose occurrences.Importantly,this treatment improved the degree of patients’satisfaction.The treatment of“8”shaped bandaging combined with spiral bandaging is worthy of clinical promotion.

Application of Serum Apelin and MMP-9 in Evaluation of Silicosis and Their Correlation with Lung Function

[Objectives]This study was conducted to explore the combination of serum Apelin and MMP-9 in the assessment of silicosis and their correlation with lung function. [Methods] From January 2020 to January 2021, eight patients with silicosis(including 3, 2 and 3 patients with silicosis in stages I, II and III respectively) were selected as the observation group, and eight persons frees of silicosis were selected as the control group during the same period. All patients were detected for serum APJ endogenous ligand(Apelin) of G protein-coupled receptor, matrix metalloproteinase-9(MMP-9) and pulmonary function indexes. The levels of serum Apelin and MMP-9 and lung function in the two groups were compared, and the correlation between serum Apelin and MMP-9 indexes and lung function was analyzed. [Results] The serum Apelin and lung function in the observation group were lower than those in the control group, and the level of MMP-9 in the observation group was higher than that in the control group, and the differences were statistically significant(P<0.05). The MMP-9 index in patients with silicosis in stage III was higher than those in patients with silicosis in stage II and stage I, and the MMP-9 index in patients with silicosis in stage II was higher than that in patients with silicosis in stage I, and the differences were statistically significant(P<0.05). The serum Apelin and lung function indexes of patients with silicosis in stage III were lower than those of patients with silicosis in stage II and stage I, and the serum Apelin and lung function indexes of patients with silicosis in stage II were lower than those of patients with silicosis in stage I, and the differences were statistically significant(P<0.05). The correlation analysis showed a positive correlation between serum Apelin and lung function indexes, and a negative correlation between MMP-9 and lung function indexes. [Conclusions] A lower serum Apelin and a higher MMP-9 value in patients with silicosis indicate severer lung function impairment, and the combined detection of serum Apelin and MMP-9 is conducive to effective evaluation of the disease of silicosis. The serum Apelin index was negatively correlated with silicosis, and the level of MMP-9 was positively correlated with silicosis, which could provide a scientific basis for clinical diagnosis and treatment.

Correlation study between pre-operative platelet aggregation rate and peri-operative blood product application in patients with off-pump coronary artery bypass grafting

Objective To explore the impact of pre-operative platelet aggregation rate(PAR)on off-pump coronary artery bypass grafting(OPCABG),meanwhile to study the relationship between platelet function and blood product application during peri-operative period in relevant patients.Methods A total of 172 patients receiving OPCABG in our hospita from 2014-01 to 2015-09 were en-

Influence of Sows Farrowing Performance on Growth of 21-Day-Old Weaned Piglets and Their Correlation Research

[ Objective ] To study the influence of sows farrowing performance on the growth of 21-day-old weaned piglets and their correlation. [ Methods ] A total of 1 350 Duroc x Landrace Yorkshire piglets produced by 2-6 fetal Landrace x Yorkshire sows from 118 litters within a month were selected to be the test objects. All the newborn piglets were lactated by their parent sows. The test groups were divided according to different numbers （6 - 17） of live birth. The variance analysis were conducted on birth weight, litter weight at birth, number of 21-day-old weaning piglets, weaning weight, weaninl2g weight of litter, weaning survival rate and relative individual weight gain among different weeks. [ Results] Sows farrowing performance had significant influence on the growth performance of 21- day-old piglets. However, the influences among different traits varied, some were even on the contrary. With the increase of the number of live birth, the birth weight, weaning weight of litter and weaning survival rate showed a decline trend. And when the number of live birth was equal or more than 14, the weaning sur- vival rate dropped to below 90% （P 〈 0.01 ） ; when the number of live birth was 13, the litter weight at birth and weaning weight of litter reached the peak. What- ever the number of live birth increased or decreased, the weaning weight showed a regular decline trend. [ Conclusions] When the number of live birth changed from 11 to 13, the maximum values of the economic indicator （weaning weight of litter） and the technical specifications （weaning survival rate） were achieved.

CTX-Ⅰ, TRACP, ADAMTS-4 and MMPs-3 expression and their correlation with different syndromes of knee osteoarthritis

Objective:To observe the expression of CTX-I(Ⅰcollagen C-terminal foreign body peptide)and TRACP(tartrate-resistant acid phosphatase)in different syndromes of knee osteoarthritis and their correlation with ADAMTS-4(polyproteoglycan enzyme-4)and MMPS-3(matrix metalloproteinase-3).Methods:A total of 98 patients diagnosed with knee osteoarthritis were enrolled from the Rehabilitation Pain Department and Rheumatology Department of Wuhan Caidian District People's Hospital.According to their syndrome types,they were divided into 40 cases of Yang deficiency and cold constipation type,28 cases of qi stagnation and blood stasis type,and 30 cases of kidney jing deficiency type.The contents of bone metabolism indexes,ADAMTS-4 and MMPS-3 in different syndromes of the three groups were compared.Correlation of bone metabolism indexes with ADAMTS-4 and MMPS-3.Results:There were statistically significant differences in the contents of CTX-I,TRACP,ADAMTS-4 and MMPS-3 among three groups with different syndromes(P<0.05).There was a positive correlation between CTX-I,TRACP,ADAMTS-4 and MMPS-3(P<0.05).The syndrome of qi stagnation and blood stasis was positively correlated with TRACP and ADAMTS-4,and negatively correlated with MMPS-3(P<0.05).The renal essence deficiency syndrome was positively correlated with CTX-I,ADAMTS-4 and MMPS-3(P<0.05).Bone metabolic indexes(CTX-I,TRACP)were positively correlated with ADAMTS-4 and MMPS-3(P<0.05).Conclusion:Different syndromes of knee osteoarthritis are correlated with bone metabolism indexes,ADAMTS-4 and MMPS-3,which can provide a certain objective basis for clinical TCM syndrome differentiation and classification。

Understanding the Correlations between Social Attention and Topic Trends of Scientific Publications

Purpose： We propose and apply a simplified nowcasting model to understand the correlations between social attention and topic trends of scientific publications. Design/methodology/approach： First, topics are generated from the obesity corpus by using the latent Dirichlet allocation （LDA） algorithm and time series of keyword search trends in Google Trends are obtained. We then establish the structural time series model using data from January 2004 to December 2012, and evaluate the model using data from January 2013. We employ a state-space model to separate different non-regression components in an observational time series （i.e. the tendency and the seasonality） and apply the ＂spike and slab prior＂ and stepwise regression to analyze the correlations between the regression component and the social media attention. The two parts are combined using Markov-chain Monte Carlo sampling techniques to obtain our results. Findings： The results of our study show that （1） the number of publications on child obesity increases at a lower rate than that of diabetes publications; （2） the number of publication on a given topic may exhibit a relationship with the season or time of year; and （3） there exists a correlation between the number of publications on a given topic and its social media attention, i.e. the search frequency related to that topic as identified by Google Trends. We found that our model is also able to predict the number of publications related to a given topic.

Study on the correlation between early three-dimensional gait analysis and clinical efficacy after robot-assisted total knee arthroplasty

Purpose:Robot-assisted technology is a forefront of surgical innovation that improves the accuracy of total knee arthroplasty (TKA).But whether the accuracy of surgery can improve the clinical efficacy still needs further research.The purpose of this study is to perform three-dimensional (3D) analysis in the early postoperative period of patients who received robot-assisted total knee arthroplasty (RATKA),and to study the trend of changes in gait parameters after RATKA and the correlation with the early clinical efficacy.Methods:Patients who received RATKA in the Center of Joint Surgery,the First Hospital Affiliated to Army Military Medical University from October 2020 to January 2021 were included.The imaging parameters,i.e.,hip-knee-ankle angle,lateral distal femoral angle,medial proximal tibial angle,posterior condylar angle were measured 3 months post-TKA.The 3D gait analysis and clinical efficacy by Western Ontario Mac Master University Index (WOMAC) score were performed pre-TKA,3 and 6 months post-TKA.The differences in spatiotemporal parameters of gait,kinetic parameters,and kinematic parameters of the operated limb and the contralateral limb were compared.The correlation between gait parameters and WOMAC scores was analyzed.Paired samplet-test and Wilcoxon rank-sum test were used to analyze the difference between groups,and Spearman correlation coefficient was used to analyze the correlation.Results:There were 31 patients included in this study,and the imaging indexes showed that all of them returned to normal post-TKA.The WOMAC score at 3 months post-TKA was significantly lower than that pre-TKA,and there was no significant difference between at 3 and 6 months.The 3D gait analysis results showed that the double support time of the operated limb reduced at 3 and 6 months (allp < 0.05),the maximum extension and maximum external rotation of the knee joint increased at stance phase,and the maximum flexion angle,the range of motion and the maximum external rotation increased at swing phase.Compared with the preoperative data,there were significant improvements (allp < 0.05).Compared with the contralateral knee joint,the maximum external rotation of the knee joint at swing phase was smaller than that of the contralateral side,and the maximum flexion and extension moment was greater than that of the contralateral knee.The maximum external rotation moment of the joint was greater than that of the contralateral knee joint (p < 0.05).There was a negative correlation between the single support time pre-TKA and the WOMAC score at 3 months (p = 0.017),and the single support time at 3 months was negatively correlated with the WOMAC score at 6 months (p = 0.043).The cadence at 6 months was negatively correlated with the WOMAC score at 6 months (p = 0.031).The maximum knee extension at stance phase at 6 months was negatively correlated with the WOMAC score at 6 month (p = 0.048).The maximum external rotation at stance phase at 6 months was negatively correlated with the WOMAC score at 6 months (p = 0.024).Conclusion:The 3D gait analysis of RATKA patients is more sensitive than WOMAC score in evaluating the clinical efficacy.Trend of changes in gait parameters shows that the knee joint support,flexion and extension function,range of motion,external rotation and varus deformity moment of the patient were significantly improved at 3 months after surgery,and continued to 6 months after surgery.Compared with the contralateral knee,the gait parameters of the operated limb still has significant gaps in functionality,such as the external rotation and flexion and extension.The single support time,cadence,knee extension,and knee external rotation of the operated limb have a greater correlation with the postoperative WOMAC score.Postoperative rehabilitation exercises should be emphasized,which is of great value for improving the early efficacy of RATKA.

Estimation of carbon pools in secondary tropical deciduous forests of Odisha,India

Secondary tropical forests sequester atmospheric CO 2 at relatively faster rates in vegetation and in soil than old-growth primary forests.Spatial understanding of biomass and carbon stocks in different plant functional types of these forests is important.Structure,diversity,composition,soil features and carbon stocks in six distinct plant functional types,namely:Moist Mixed-Deciduous Forest,Peninsular Sal Forest(PSF),Semi-Evergreen Forest(SEF),Planted Teak Forest,Bamboo Brakes(BB),and Degraded Thorny Shrubby Forest were quantified as secondary tropical deciduous forests of the Chandaka Wildlife Sanctuary,Eastern Ghats of Odisha,India.Seventy-one species≥10 cm Girth at breast height(GBH)were recorded,belonging to 38 families and 65 genera.Above-ground biomass carbon and soil organic carbon ranged from 2.1–72.7 Mg C ha^(−1) and 20.6–67.1 Mg C ha^(−1),respectively,among all plant functional types.Soil organic carbon and important value index were positively correlated with above-ground biomass carbon.Maximum carbon allocation was in SOC pool(51–91%),followed by the above-ground biomass pool(9–52%),indicating SOC is one of the major carbon sinks in secondary dry forests.The results highlight the importance of secondary tropical deciduous forests in biodiversity conservation and ecological importance in reducing greenhouse gases.

Progression-free survival as surrogate endpoint in advanced pancreatic cancer: meta-analysis of 30 randomized first-line trials

BACKGROUND：Progression-free survival（PFS）has not been extensively investigated as a surrogate for survival in the firstline treatments of pancreatic cancer.The aim of this review was to evaluate PFS as a potential surrogate endpoint for overall survival（OS）in advanced pancreatic cancer in trials comparing poly-chemotherapy to gemcitabine alone.DATA SOURCES： A systematic literature search in PubMed, Embase, Web of Science, and the Cochrane Central Register of Controlled Trials was conducted. The key words included randomized trial, first-line chemotherapy, pancreatic cancer, gemcitabine and poly-chemotherapy. Adjusted weighted linear regression was used to calculate Rs （Spearman＇s rank-order correlation coefficient） between PFS and post-progression survival （PPS） with OS （Rs） and between treatment effects on PFS and OS （RHR）. RESUEFS： A total of 30 trials including 8467 patients met the inclusion criteria. Correlation between the treatment effects on PFS and OS （RHR=0.78） and between the endpoint PFS and OS was high across all studies （Rs=0.75）. The slope of the re- gression line was 0.76±0.26, indicating that an agent produc- ing a 10% risk reduction for PFS will provide a 7.6%±2.6% risk reduction for OS. Correlation between PPS and OS was very strong （Rs=0.71） and accounted for more than 50% of the whole OS variability （R2=0.57）. CONCLUSION： Because of the robust correlation with OS and the potential influence of PPS caused by the second line therapies, it may be justified to consider PFS as a surrogate endpoint in trials evaluating new cytotoxic agents when gemcitabine is the control arm.

The study of the correlation between serum uric acid and insulin resistance in middle-aged and elderly hospitalized patients with type 2 diabetes

Objective To study the relationship between serum uric acid and insulin resistance in type 2 diabetic patients.Methods A total of 728 middle-aged and elderly type 2 diabetic patients were recruited and the anthropometric,clinical and biochemical parameters and

DESI Legacy Imaging Surveys Data Release 9:Cosmological constraints from galaxy clustering and weak lensing using the minimal bias model

We present a tentative constraint on cosmological parameters Ω_(m) and σ_(8) from a joint analysis of galaxy clustering and galaxygalaxy lensing from DESI Legacy Imaging Surveys Data Release 9(DR9),covering approximately 10000 square degrees and spanning the redshift range of 0.1 to 0.9.To study the dependence of cosmological parameters on lens redshift,we divide lens galaxies into seven approximately volume-limited samples,each with an equal width in photometric redshift.To retrieve the intrinsic projected correlation function w_(p)(r_(p))from the lens samples,we employ a novel method to account for redshift uncertainties.Additionally,we measured the galaxy-galaxy lensing signal ΔΣ(r_(p))for each lens sample,using source galaxies selected from the shear catalog by applying our Fourier Quad pipeline to DR9 images.We model these observables within the flatΛCDM framework,employing the minimal bias model.To ensure the reliability of the minimal bias model,we apply conservative scale cuts:r_(p)>8 and 12 h^(-1)Mpc,for w_(p)(r_(p))and ΔΣ(r_(p)),respectively.Our findings suggest a mild tendency that S_(8)=σ_(8)√Ω_(m)/0.3increases with lens redshift,although this trend is only marginally significant.When we combine low redshift samples,the value of S8is determined to be 0.84±0.02,consistent with the Planck results but significantly higher than the 3×2 pt analysis by 2-5σ.Despite the fact that further refinements in measurements and modeling could improve the accuracy of our results,the consistency with standard values demonstrates the potential of our method for more precise and accurate cosmology in the future.

Potassium carbonate-based ternary transition temperature mixture(deep eutectic analogues)for CO_(2)absorption:Characterizations and DFT analysis

Is it possible to improve CO_(2)solubility in potassium carbonate(K_(2)CO_(3))-based transition temperature mixtures(TTMs)?To assess this possibility,a ternary transition-temperature mixture(TTTM)was prepared by using a hindered amine,2-amino-2-methyl-1,3-propanediol(AMPD).Fourier transform infrared spectroscopy(FT-IR)was employed to detect the functional groups including hydroxyl,amine,carbonate ion,and aliphatic functional groups in the prepared solvents.From thermogravimetric analysis(TGA),it was found that the addition of AMPD to the binary mixture can increase the thermal stability of TTTM.The viscosity findings showed that TTTM has a higher viscosity than TTM while their difference was decreased by increasing temperature.In addition,Eyring’s absolute rate theory was used to compute the activation parameters(∆G^(*),∆H^(*),and ∆S^(*)).The CO_(2)solubility in liquids was measured at a temperature of 303.15 K and pressures up to 1.8 MPa.The results disclosed that the CO_(2)solubility of TTTM was improved by the addition of AMPD.At the pressure of about 1.8 MPa,the CO_(2)mole fractions of TTM and TTTM were 0.1697 and 0.2022,respectively.To confirm the experimental data,density functional theory(DFT)was employed.From the DFT analysis,it was found that the TTTM+CO_(2)system has higher interaction energy(|∆E|)than the TTM+CO_(2)system indicating the higher CO_(2)affinity of the former system.This study might help scientists to better understand and to improve CO_(2)solubility in these types of solvents by choosing a suitable amine as HBD and finding the best combination of HBA and HBD.