BACKGROUND： Corticotrophin releasing hormone （CRH） is believed to mediate stress-induced behaviors, implying a broader, integrative role for the hormone in the psychological stress response, and studies on CRH in p...BACKGROUND： Corticotrophin releasing hormone （CRH） is believed to mediate stress-induced behaviors, implying a broader, integrative role for the hormone in the psychological stress response, and studies on CRH in physical stress are few. This study was undertaken to investigate whether CRH plays an important role in cerebral infarction-related gastrointestinal barrier dysfunction.METHODS： Thirty male Wistar rats were randomly divided into a pseudo-operation group （group C, n=10）, a cerebral infarction group （group I, n=10）, and a cerebral infarction ＋ ic α-helical-CRH （9-41） group （group Aic, n=10）. Urine samples were collected to determine the levels of epinephrine, norepinephrine, cortisol, and sucrose. At 24 hours after establishment of the models, blood samples were taken to determine the activity of diamine oxidase （DAO） and the concentration of D-lactic acid （D-lac）. The stomach was taken to determine gastric Guth score, and the hypothalamus was also taken to determine tissue CRH protein expression using Western blotting.RESULTS： The hypothalamus CRH protein, the indicators of stress, the plasma DAO activity and plasma D-lac, urine sucrose exertion and gastric Guth score in group I were higher than those in groups Aic and C.CONCLUSIONS： After cerebral infarction, CRH in the hypothalamus was increased, the hypothalamic-pituitary-adrenal axis and the sympathetic nervous system were activated, gastrointestinal permeability was increased, and gastrointestinal barrier function was destroyed. CRH receptor antagonist alleviated the gastrointestinal barrier function.展开更多
目的:探讨肾上腺切除幼鼠癫痫易感性及对海马促肾上腺皮质激素释放激素(CRH)m RNA表达的影响。方法:60只Wistar P10幼鼠随机均分为空白对照组、肾上腺切除组、假手术组。手术次日腹腔注射NMDA 7 mg/kg诱发抽搐,评价发作潜伏期(min)及程...目的:探讨肾上腺切除幼鼠癫痫易感性及对海马促肾上腺皮质激素释放激素(CRH)m RNA表达的影响。方法:60只Wistar P10幼鼠随机均分为空白对照组、肾上腺切除组、假手术组。手术次日腹腔注射NMDA 7 mg/kg诱发抽搐,评价发作潜伏期(min)及程度。原位杂交方法检测海马CRH m RNA表达。结果:空白对照组潜伏期、评分为(43.65±2.96)min、4.40±0.60,肾上腺切除组为(35.05±2.35)min、5.56±0.76,假手术组为(42.60±1.90)min、4.55±0.76。肾上腺切除组与其他2组比较,潜伏期、评分差异具有显著性(F=73.73,P<0.05;F=15.52,P<0.05),3组幼鼠海马CRH m RNA阳性表达率为20%、55%、15%,与其他2组相比,肾上腺切除组海马CRH m RNA增加具有显著意义(χ2=9.048,P<0.05)。结论 :肾上腺切除幼鼠潜伏期缩短、评分增加,海马CRH m RNA提高,提示肾上腺切除幼鼠对致痫剂NMDA敏感性增高。展开更多
目的:探讨舒肝健脑调郁片的抗抑郁机制,为临床应用提供依据。方法:通过强迫游泳不动时间、糖水消耗量等方法评价大鼠模型行为学指标变化、采用透射电镜观察细胞超微结构和RT-PCR技术研究下丘脑促肾上腺皮质激素释放激素(corticotroph in...目的:探讨舒肝健脑调郁片的抗抑郁机制,为临床应用提供依据。方法:通过强迫游泳不动时间、糖水消耗量等方法评价大鼠模型行为学指标变化、采用透射电镜观察细胞超微结构和RT-PCR技术研究下丘脑促肾上腺皮质激素释放激素(corticotroph in releasing hormone,CRH)基因表达的影响。结果:舒肝健脑调郁片能够减轻应激造成的海马神经元损伤,对抑郁症大鼠的脑损伤有保护作用。舒肝健脑调郁片可使模型大鼠下丘脑CRH基因表达下降,与模型组比较差异有显著性(P<0.05)。表明舒肝健脑调郁片可通过下调CRHmRNA表达,抑制HPA轴功能亢进,达到抗抑郁作用。展开更多
文摘BACKGROUND： Corticotrophin releasing hormone （CRH） is believed to mediate stress-induced behaviors, implying a broader, integrative role for the hormone in the psychological stress response, and studies on CRH in physical stress are few. This study was undertaken to investigate whether CRH plays an important role in cerebral infarction-related gastrointestinal barrier dysfunction.METHODS： Thirty male Wistar rats were randomly divided into a pseudo-operation group （group C, n=10）, a cerebral infarction group （group I, n=10）, and a cerebral infarction ＋ ic α-helical-CRH （9-41） group （group Aic, n=10）. Urine samples were collected to determine the levels of epinephrine, norepinephrine, cortisol, and sucrose. At 24 hours after establishment of the models, blood samples were taken to determine the activity of diamine oxidase （DAO） and the concentration of D-lactic acid （D-lac）. The stomach was taken to determine gastric Guth score, and the hypothalamus was also taken to determine tissue CRH protein expression using Western blotting.RESULTS： The hypothalamus CRH protein, the indicators of stress, the plasma DAO activity and plasma D-lac, urine sucrose exertion and gastric Guth score in group I were higher than those in groups Aic and C.CONCLUSIONS： After cerebral infarction, CRH in the hypothalamus was increased, the hypothalamic-pituitary-adrenal axis and the sympathetic nervous system were activated, gastrointestinal permeability was increased, and gastrointestinal barrier function was destroyed. CRH receptor antagonist alleviated the gastrointestinal barrier function.
文摘目的:探讨肾上腺切除幼鼠癫痫易感性及对海马促肾上腺皮质激素释放激素(CRH)m RNA表达的影响。方法:60只Wistar P10幼鼠随机均分为空白对照组、肾上腺切除组、假手术组。手术次日腹腔注射NMDA 7 mg/kg诱发抽搐,评价发作潜伏期(min)及程度。原位杂交方法检测海马CRH m RNA表达。结果:空白对照组潜伏期、评分为(43.65±2.96)min、4.40±0.60,肾上腺切除组为(35.05±2.35)min、5.56±0.76,假手术组为(42.60±1.90)min、4.55±0.76。肾上腺切除组与其他2组比较,潜伏期、评分差异具有显著性(F=73.73,P<0.05;F=15.52,P<0.05),3组幼鼠海马CRH m RNA阳性表达率为20%、55%、15%,与其他2组相比,肾上腺切除组海马CRH m RNA增加具有显著意义(χ2=9.048,P<0.05)。结论 :肾上腺切除幼鼠潜伏期缩短、评分增加,海马CRH m RNA提高,提示肾上腺切除幼鼠对致痫剂NMDA敏感性增高。
文摘目的:探讨舒肝健脑调郁片的抗抑郁机制,为临床应用提供依据。方法:通过强迫游泳不动时间、糖水消耗量等方法评价大鼠模型行为学指标变化、采用透射电镜观察细胞超微结构和RT-PCR技术研究下丘脑促肾上腺皮质激素释放激素(corticotroph in releasing hormone,CRH)基因表达的影响。结果:舒肝健脑调郁片能够减轻应激造成的海马神经元损伤,对抑郁症大鼠的脑损伤有保护作用。舒肝健脑调郁片可使模型大鼠下丘脑CRH基因表达下降,与模型组比较差异有显著性(P<0.05)。表明舒肝健脑调郁片可通过下调CRHmRNA表达,抑制HPA轴功能亢进,达到抗抑郁作用。
