Extraction Process Optimization and Moisturizing Performance of Fucoidan from Sargassum fusiforme

[Objectives] The research aimed to optimize extraction process of fucoidan from Sargassum fusiforme( FSF) and study its moisturizing performance. [Methods]Extracting condition of FSF by cellulase hydrolysis-ultrasonic assisted extraction method was optimized. The influences of solvent p H,enzyme dosage,extraction temperature,cellulose hydrolysis time,ultrasonication time,and the ratio of material to liquid on FSF were investigated by single factor and orthogonal experiments. [Results] The optimum extraction conditions were as followings:p H,4. 5; enzyme dosage,1%; extraction temperature,40℃; cellulose hydrolysis time,2 h; ultrasonic time,15 min; and the ratio of material to liquid,1∶ 10( g∶ m L). Under the optimal condition,the extraction yield of FSF was 8. 50%,RSD = 2. 74%. The short-time hygroscopicity( within 8 h) of crude extract of fucoidan from S. fusiforme( CEFSF) was better than glycerin,butanediol,and sodium alginate,and the moisture retention capacity of 1% CEFSF aqueous solution was better than 1% butanediol or 1% sodium alginate,and was equal to 5% glycerin under relative humidity of 43% and 81%. The determination results of skin moisture content and transepidermal water loss rate( TEWL)showed that: 5% CEFSF solution had good moisturizing effect. [Conclusions]The research could provide certain reference for deep development of S. fusiforme.

Study of a Whitening and Anti-wrinkle Bio-functional Ingredient Based on Epigenetics

Via quantitative polymerase chain reaction （qPCR） test method, the efficacy of hexapeptide-2 was studied, including silencing on miR-29 and increasing miR-218 expression which targeted collagen coding mRNA （message RNA） then decreases the collagen synthesis, and the latter silences the melanin synthesis related enzyme. Then the double blind spilt-face clinical test on 35 Asian people （18 male and 17 female） was conducted to evaluate the whitening and anti-wrinkle effect. The qPCR test showed that hexapeptide-2 can decrease miR-29 expression for human fibroblast of different age （P 〈 0.05）; and the result on human melanocyte showed that it can increase miR-218 expression （P 〈 0.05）. Immunostaining results showed that it can increase the expression of collagen I and III significantly （P 〈 0.05）. The double blind split - face test showed that 1% hexapeptide-2 displays significant efficacy for skin whitening, spots lightening, skin erythema and skin dullness improvement, as well as skin wrinkles reduction as comparing with that from the placebo side （P 〈 0.05）.