In this study,a natural cotton thread immunoassay device combined with gold nanorod（GNR） reporter probe is developed for the rapid,sensitive and quantitative electrochemical determination of human ferritin,a lung ca...In this study,a natural cotton thread immunoassay device combined with gold nanorod（GNR） reporter probe is developed for the rapid,sensitive and quantitative electrochemical determination of human ferritin,a lung cancer related biomarker.Human ferritin as an analyte and a pair of monoclonal antibodies are used to demonstrate the proof-of-concept on the cotton thread immunoassay device.An enhancement of the sensitivity is achieved by using gold nanorod as an electroactive report probe compared with a traditional gold nanoparticle（GNP） report probe.The device was capable of measuring 1.58 ng/mL ferritin in 30 min by anodic stripping voltammetry（ASV） testing,which meet the requirement for clinical diagnosis.展开更多
目的:比较传统棉线结扎与种植体周围局部注射牙龈卟啉单胞菌脂多糖(lipopolysaccharide,LPS)诱导法,以及两种方法联合建立大鼠种植体周炎模型的效率和效果,以探索大鼠种植体周炎模型建模的最佳方法。方法:纳入39只雄性SD大鼠,拔除上颌...目的:比较传统棉线结扎与种植体周围局部注射牙龈卟啉单胞菌脂多糖(lipopolysaccharide,LPS)诱导法,以及两种方法联合建立大鼠种植体周炎模型的效率和效果,以探索大鼠种植体周炎模型建模的最佳方法。方法:纳入39只雄性SD大鼠,拔除上颌左侧第一磨牙,拔牙窝愈合4周后植入钛种植体。种植体植入4周骨结合后,将39只大鼠随机分为4组,分别采取棉线结扎(n=12)、种植体周围局部注射LPS(n=12),以及两种方法联合(n=12)诱导大鼠种植体周炎,同时设无任何处理对照组(n=3)。记录实验组大鼠诱导前及诱导后2周和4周时的探诊深度(probing depth,PD)、探诊出血(bleeding on probing,BOP)和牙龈指数(gingival index,GI)。收集种植体周围牙龈组织,提取RNA,实时荧光定量分析(real time quantitative PCR,RT-qPCR)检测炎症因子白细胞介素-1β(interleukin-1β,IL-1β)和肿瘤坏死因子-α(tumor necrosis factor-alpha,TNF-α)表达。分别于诱导2周和4周时处死实验组动物,收集上颌骨,采用微型CT(micro-CT)观察种植体边缘骨吸收状况。结果:种植4周后,种植体骨结合良好。3个实验组均可见牙龈红肿,质软,种植体边缘骨吸收。诱导2周和4周时,各实验组PD、GI和BOP均较诱导前增加,但仅BOP在3组间差异有统计学意义(P<0.05)。炎症诱导2周时,棉线结扎组和联合组在每个位点均可见边缘骨吸收,且联合组每个位点边缘骨吸收程度均大于棉线结扎组,但差异不具有统计学意义(P>0.05);局部注射组部分种植体的部分位点可见边缘骨吸收。炎症诱导4周时,各实验组各位点均可见边缘骨吸收,其中棉线结扎组和联合组边缘骨吸收大于局部注射组,差异有统计学意义(P<0.05)。诱导2周和4周时,实验组TNF-α和IL-1β均较对照组表达增加,差异有统计学意义(P<0.05)。结论:相比于种植体周围局部注射LPS,棉线结扎及两种方法联合可以更好且更快地诱导大鼠种植体周炎。展开更多
In order to achieve an innovative strategy to renew the biomass of Phanerochaete chrysosporium in an immobilized growth system which can maintain white-rot fungi biomass, a novel knotted cotton-thread carrier was desi...In order to achieve an innovative strategy to renew the biomass of Phanerochaete chrysosporium in an immobilized growth system which can maintain white-rot fungi biomass, a novel knotted cotton-thread carrier was designed and made. By using a high-speed stirring apparatus under the conditions of 1400 r/min stirring speed for 6 min, mycelia immobilized on the knotted cotton-thread carriers were exfoliated completely and homogenized to a proper size. Furthermore, the homogenized mycelia from the immobilized mycelia can resume their growth on the knotted cotton-thread carriers in agitated flask cultures. The average regrowth biomass on the new carriers and the reused carriers was 0.0171 g/carrier and 0.0314 g/carrier, respectively. It proves that the knotted cotton-thread carrier performs perfectly in homogening the immobilized mycelia to achieve the biomass renewal of P. chrysosporium in an immobilized growth system.展开更多
基金financially supported by the National Natural Science Foundation of China (No. 21205094)NFFTBS (Nos. J1103311,J1210057)the New Faculty Startup Funds of Northwest University in Shaanxi Province (No. PR12011)
文摘In this study,a natural cotton thread immunoassay device combined with gold nanorod（GNR） reporter probe is developed for the rapid,sensitive and quantitative electrochemical determination of human ferritin,a lung cancer related biomarker.Human ferritin as an analyte and a pair of monoclonal antibodies are used to demonstrate the proof-of-concept on the cotton thread immunoassay device.An enhancement of the sensitivity is achieved by using gold nanorod as an electroactive report probe compared with a traditional gold nanoparticle（GNP） report probe.The device was capable of measuring 1.58 ng/mL ferritin in 30 min by anodic stripping voltammetry（ASV） testing,which meet the requirement for clinical diagnosis.
文摘目的:比较传统棉线结扎与种植体周围局部注射牙龈卟啉单胞菌脂多糖(lipopolysaccharide,LPS)诱导法,以及两种方法联合建立大鼠种植体周炎模型的效率和效果,以探索大鼠种植体周炎模型建模的最佳方法。方法:纳入39只雄性SD大鼠,拔除上颌左侧第一磨牙,拔牙窝愈合4周后植入钛种植体。种植体植入4周骨结合后,将39只大鼠随机分为4组,分别采取棉线结扎(n=12)、种植体周围局部注射LPS(n=12),以及两种方法联合(n=12)诱导大鼠种植体周炎,同时设无任何处理对照组(n=3)。记录实验组大鼠诱导前及诱导后2周和4周时的探诊深度(probing depth,PD)、探诊出血(bleeding on probing,BOP)和牙龈指数(gingival index,GI)。收集种植体周围牙龈组织,提取RNA,实时荧光定量分析(real time quantitative PCR,RT-qPCR)检测炎症因子白细胞介素-1β(interleukin-1β,IL-1β)和肿瘤坏死因子-α(tumor necrosis factor-alpha,TNF-α)表达。分别于诱导2周和4周时处死实验组动物,收集上颌骨,采用微型CT(micro-CT)观察种植体边缘骨吸收状况。结果:种植4周后,种植体骨结合良好。3个实验组均可见牙龈红肿,质软,种植体边缘骨吸收。诱导2周和4周时,各实验组PD、GI和BOP均较诱导前增加,但仅BOP在3组间差异有统计学意义(P<0.05)。炎症诱导2周时,棉线结扎组和联合组在每个位点均可见边缘骨吸收,且联合组每个位点边缘骨吸收程度均大于棉线结扎组,但差异不具有统计学意义(P>0.05);局部注射组部分种植体的部分位点可见边缘骨吸收。炎症诱导4周时,各实验组各位点均可见边缘骨吸收,其中棉线结扎组和联合组边缘骨吸收大于局部注射组,差异有统计学意义(P<0.05)。诱导2周和4周时,实验组TNF-α和IL-1β均较对照组表达增加,差异有统计学意义(P<0.05)。结论:相比于种植体周围局部注射LPS,棉线结扎及两种方法联合可以更好且更快地诱导大鼠种植体周炎。
基金financially supported by Chongqing Natural Science Foundation (cstc2019jcyj-msxmX0314)Innovation Teams in Colleges and Universities of Chongqing (CXQT20005)Chongqing Engineering Research Center for Micro-Nano Biomedical Materials and Devices
基金Supported by the National Natural Science Foundation of China (Grant No. 206770 33)
文摘In order to achieve an innovative strategy to renew the biomass of Phanerochaete chrysosporium in an immobilized growth system which can maintain white-rot fungi biomass, a novel knotted cotton-thread carrier was designed and made. By using a high-speed stirring apparatus under the conditions of 1400 r/min stirring speed for 6 min, mycelia immobilized on the knotted cotton-thread carriers were exfoliated completely and homogenized to a proper size. Furthermore, the homogenized mycelia from the immobilized mycelia can resume their growth on the knotted cotton-thread carriers in agitated flask cultures. The average regrowth biomass on the new carriers and the reused carriers was 0.0171 g/carrier and 0.0314 g/carrier, respectively. It proves that the knotted cotton-thread carrier performs perfectly in homogening the immobilized mycelia to achieve the biomass renewal of P. chrysosporium in an immobilized growth system.
