Ribosome-like particles have been found in the proplastids in young cotyledon cells of lotus (%Nelumbo nucifera Gaertn% L.). Following the development of young embryo, some lamellar structures and tubular complex occu...Ribosome-like particles have been found in the proplastids in young cotyledon cells of lotus (%Nelumbo nucifera Gaertn% L.). Following the development of young embryo, some lamellar structures and tubular complex occurred in the plastids in young cotyledon cells, and some ribosome-like particles appeared in the loose region of these membrane system and stroma. About 15- 20 d after fertilization, with the further development of plastid, a large number of starch and DNA were synthesized in the plastids, and the plastids contained abundant and clear morphologically ribosomes, some of which presented spiral structure. About 16-18 d after fertilization, amyloplasts were isolated and purified from cotyledon of lotus, and ribosomes bands were obtained by use of sucrose density gradient centrifugation of ribosomes isolated from amyloplasts. RNA and protein contents of ribosomes have also been determined.展开更多
Polyamines are small nitrogen-containing organic molecules, which are widely distributed in plants. They are involved in the regulation of normal plant growth and developmental processes. In this study we examined the...Polyamines are small nitrogen-containing organic molecules, which are widely distributed in plants. They are involved in the regulation of normal plant growth and developmental processes. In this study we examined the role of polyamines on the growth of Cucurbita pepo L.(zucchini) cotyledons incubated on solutions of different types of cytokinins (BA—N6-benzylaminopurine or 4PU-30—N1-(2-chloro-4-pyridyl)-N2-phenylurea) and copper in excess. We found that endogenous polyamines, and mainly the conjugated fraction, are involved in the cell division processes of isolated zucchini cotyledons and their changes are related to the specific action of the used growth regulating factors.展开更多
莲(Nelumbo nucifera Gaertn.)幼胚子叶细胞造淀粉体和分离的造粉质体上均呈现强烈的 Feulgen 反应物质。经 DNA 酶处理后,在子叶细胞造淀粉体上呈 Feulgen 负反应。将分离的造粉质体用特异性的 DNA 荧光染料 DAPI 染色,造粉质体显示蓝...莲(Nelumbo nucifera Gaertn.)幼胚子叶细胞造淀粉体和分离的造粉质体上均呈现强烈的 Feulgen 反应物质。经 DNA 酶处理后,在子叶细胞造淀粉体上呈 Feulgen 负反应。将分离的造粉质体用特异性的 DNA 荧光染料 DAPI 染色,造粉质体显示蓝色的荧光。实验证明,莲幼胚子叶细胞随着发育时期的增长,造粉质体 DNA 的含量逐渐增加,显示出有规律的动态变化过程。在电镜下观察,造粉质体 DNA 区域外无膜结构存在,故具有原核生物的特征。这种质体 DNA 的纤维丝直径大约为25。展开更多
文摘Ribosome-like particles have been found in the proplastids in young cotyledon cells of lotus (%Nelumbo nucifera Gaertn% L.). Following the development of young embryo, some lamellar structures and tubular complex occurred in the plastids in young cotyledon cells, and some ribosome-like particles appeared in the loose region of these membrane system and stroma. About 15- 20 d after fertilization, with the further development of plastid, a large number of starch and DNA were synthesized in the plastids, and the plastids contained abundant and clear morphologically ribosomes, some of which presented spiral structure. About 16-18 d after fertilization, amyloplasts were isolated and purified from cotyledon of lotus, and ribosomes bands were obtained by use of sucrose density gradient centrifugation of ribosomes isolated from amyloplasts. RNA and protein contents of ribosomes have also been determined.
文摘Polyamines are small nitrogen-containing organic molecules, which are widely distributed in plants. They are involved in the regulation of normal plant growth and developmental processes. In this study we examined the role of polyamines on the growth of Cucurbita pepo L.(zucchini) cotyledons incubated on solutions of different types of cytokinins (BA—N6-benzylaminopurine or 4PU-30—N1-(2-chloro-4-pyridyl)-N2-phenylurea) and copper in excess. We found that endogenous polyamines, and mainly the conjugated fraction, are involved in the cell division processes of isolated zucchini cotyledons and their changes are related to the specific action of the used growth regulating factors.
文摘莲(Nelumbo nucifera Gaertn.)幼胚子叶细胞造淀粉体和分离的造粉质体上均呈现强烈的 Feulgen 反应物质。经 DNA 酶处理后,在子叶细胞造淀粉体上呈 Feulgen 负反应。将分离的造粉质体用特异性的 DNA 荧光染料 DAPI 染色,造粉质体显示蓝色的荧光。实验证明,莲幼胚子叶细胞随着发育时期的增长,造粉质体 DNA 的含量逐渐增加,显示出有规律的动态变化过程。在电镜下观察,造粉质体 DNA 区域外无膜结构存在,故具有原核生物的特征。这种质体 DNA 的纤维丝直径大约为25。
基金supported by National Natural Science Foundation of China (30671315)Heilongjiang Province Natural Science Funds for Distinguished Young Scholar (JC2OO617),and the Massachusetts Agricultural Experiment Station, University of Massachusetts Amherst
