本研究旨在分析长沙市四棘食道口线虫分离株线粒体细胞色素c氧化酶第Ⅰ亚基(cox1)基因部分序列(pcox1)的遗传变异情况。应用聚合酶链反应(PCR)扩增四棘食道口线虫虫株的pcox1,应用Clustal X 1.83程序对序列进行比对,同时利用DNAStar 5....本研究旨在分析长沙市四棘食道口线虫分离株线粒体细胞色素c氧化酶第Ⅰ亚基(cox1)基因部分序列(pcox1)的遗传变异情况。应用聚合酶链反应(PCR)扩增四棘食道口线虫虫株的pcox1,应用Clustal X 1.83程序对序列进行比对,同时利用DNAStar 5.0中的MegAlign程序进行同源性分析,并与GenBankTM中已知四棘食道口线虫相应基因序列进行比较分析。所得pcox1序列长度一致,均为393 bp,与GenBank公布的线虫相关序列进行比较分析结果表明,各个分离株与已知四棘食道口线虫相应基因的相似性分别在98%以上,与其它科线虫的相似性均小于91%。四棘食道口线虫pcox1序列种内相对保守,种间差异明显。本研究为进一步研究四棘食道口线虫的群体遗传学奠定了基础。展开更多
[Objective] The research aimed at cloning and analyzing mitochondrial cytochrome oxidase I gene（cox 1）of C.suppressalis.[Method] The mitochondrial cox 1 gene of C.suppressalis was cloned with PCR method and sequence...[Objective] The research aimed at cloning and analyzing mitochondrial cytochrome oxidase I gene（cox 1）of C.suppressalis.[Method] The mitochondrial cox 1 gene of C.suppressalis was cloned with PCR method and sequenced.Then,cox1 sequences of other 21 Lepidopteran species were obtained by blasting the GenBank with cox 1 gene sequence of C.suppressalis.Finally,homology comparison and molecular phylogenitic analysis among the 22 Lepidopteran species were conducted.[Result] The open reading frame of cox 1 gene from C.suppressalis contained 1 531 nucleotides encoding a putative protein of 510 amino acids.The cox1 gene used a start codon CGA,and an incomplete termination codon composed of only T.Based on the amino acid sequences of cox 1,the molecular phylogenetic tree of Lepidoptera was reconstructed using the maximum likelihood（ML）method.The molecular phylogenetic tree was similar to the morphological phylogenetic tree mainly,but also showed some differences.[Conclusion] The result will provide reference for further research on expression and application of cox 1 gene.展开更多
文摘以从中国甘肃玛曲牦牛胆管中采集的3条中华双腔吸虫作为研究对象,用引物JB3及JB4.5扩增中华双腔吸虫的线粒体细胞色素c氧化酶第Ⅰ亚基(cox1)基因部分序列(pcox1),并用pcox1序列构建其与其它吸虫的进化关系。将测定获得序列应用Clustal X 1.81程序进行比对,然后用Phylip 3.67程序MP法,并用Puzzle 5.2程序构建最大似然树。所获得的3个中华双腔吸虫样品pcox1序列长度一致,均为355bp。种系发育分析表明,3个中华双腔吸虫样品位于同一分枝。本研究系首次报道中华双腔吸虫的线粒体cox1序列,从而为进一步研究中华双腔吸虫进一步的分类、鉴定和遗传变异研究奠定了基础。
文摘本研究旨在分析长沙市四棘食道口线虫分离株线粒体细胞色素c氧化酶第Ⅰ亚基(cox1)基因部分序列(pcox1)的遗传变异情况。应用聚合酶链反应(PCR)扩增四棘食道口线虫虫株的pcox1,应用Clustal X 1.83程序对序列进行比对,同时利用DNAStar 5.0中的MegAlign程序进行同源性分析,并与GenBankTM中已知四棘食道口线虫相应基因序列进行比较分析。所得pcox1序列长度一致,均为393 bp,与GenBank公布的线虫相关序列进行比较分析结果表明,各个分离株与已知四棘食道口线虫相应基因的相似性分别在98%以上,与其它科线虫的相似性均小于91%。四棘食道口线虫pcox1序列种内相对保守,种间差异明显。本研究为进一步研究四棘食道口线虫的群体遗传学奠定了基础。
基金Supported by New Century Program for Excellent Talents of Ministry of Education of China(NCET-07-0251)Talents Foundation of Anhui Province(08040106803)~~
文摘[Objective] The research aimed at cloning and analyzing mitochondrial cytochrome oxidase I gene（cox 1）of C.suppressalis.[Method] The mitochondrial cox 1 gene of C.suppressalis was cloned with PCR method and sequenced.Then,cox1 sequences of other 21 Lepidopteran species were obtained by blasting the GenBank with cox 1 gene sequence of C.suppressalis.Finally,homology comparison and molecular phylogenitic analysis among the 22 Lepidopteran species were conducted.[Result] The open reading frame of cox 1 gene from C.suppressalis contained 1 531 nucleotides encoding a putative protein of 510 amino acids.The cox1 gene used a start codon CGA,and an incomplete termination codon composed of only T.Based on the amino acid sequences of cox 1,the molecular phylogenetic tree of Lepidoptera was reconstructed using the maximum likelihood（ML）method.The molecular phylogenetic tree was similar to the morphological phylogenetic tree mainly,but also showed some differences.[Conclusion] The result will provide reference for further research on expression and application of cox 1 gene.
