【目的】观察青藤碱(sinomenine,SIN)对环氧化酶2(COX2)、α7烟碱型乙酰胆能受体(α7nAChR)和腺苷受体(A2A)表达变化的影响,探讨青藤碱对A549细胞增殖的抑制作用及机制。【方法】采用四甲基偶氮唑盐微量酶反应比色(MTT)法检测青藤碱和4...【目的】观察青藤碱(sinomenine,SIN)对环氧化酶2(COX2)、α7烟碱型乙酰胆能受体(α7nAChR)和腺苷受体(A2A)表达变化的影响,探讨青藤碱对A549细胞增殖的抑制作用及机制。【方法】采用四甲基偶氮唑盐微量酶反应比色(MTT)法检测青藤碱和4-甲基亚硝胺基-1-3-吡啶基-1-丁酮(NNK)对A549细胞增殖的影响;细胞划痕实验观察青藤碱和NNK对A549细胞迁移的影响;Western blot法观察青藤碱和NNK对A549细胞COX2蛋白表达的影响;RT-PCR和Western blot法观察青藤碱和NNK对A549细胞α7n ACh R、A2A表达的影响。【结果】NNK能促进增殖和迁移,青藤碱能抑制A549细胞增殖和迁移;NNK组COX2蛋白质水平增加,青藤碱组COX2蛋白质水平下降;NNK组α7n ACh R、A2A的表达增加,青藤碱组α7n ACh R、A2A表达下降。【结论】青藤碱能通过抑制COX2蛋白表达发挥抗A549细胞增殖和迁移作用;青藤碱对α7n ACh R和A2A受体的表达均有抑制作用。展开更多
Aim： To investigate the regulatory function of Cox7a2 on steroidogenesis and the mechanism involved in TM3 mouse Leydig cells. Methods： The cDNA of Cox7a2 was cloned from TM3 mouse Leydig cells. It was subcloned to ...Aim： To investigate the regulatory function of Cox7a2 on steroidogenesis and the mechanism involved in TM3 mouse Leydig cells. Methods： The cDNA of Cox7a2 was cloned from TM3 mouse Leydig cells. It was subcloned to pDsRed- Express-N 1 and transfected back into TM3 mouse Leydig cells for Cox7a2 overexpression by transient gene transfection. Steroidogenesis affected by overexpressed Cox7a2 was studied by ELISA. To elicit the mechanism of this effect, expression of steroidogenic acute regulatory （STAR） protein and reactive oxygen species （ROS） were examined by Western blot and fluorometer, respectively. Results： The cDNA of Cox7a2 （249 bp） was cloned from Leydig cells and confirmed by DNA sequencing. After constructed pDsRed-Express-N1-Cox7a2 was transfected back into TM3 mouse Leydig cells, Cox7a2 inhibited not only luteinizing hormone （LH）-induced secretion of testosterone but also the expression of StAR protein. At the same time, Cox7a2 increased the activity of ROS in TM3 mouse Leydig cells. Conclusion： Cox7a2 inhibited LH-induced StAR protein expression, and consequent testosterone production, at least in part, by increasing ROS activity in TM3 mouse Leydig cells.展开更多
Objective: To observe cyclooxygenase (COX)-2 expression in normal oral mucosa (NOM), oral lichen planus (OLP) and oral squamous cell carcinoma (OSCC) and explore its significance in the incidence of oral cancer. Metho...Objective: To observe cyclooxygenase (COX)-2 expression in normal oral mucosa (NOM), oral lichen planus (OLP) and oral squamous cell carcinoma (OSCC) and explore its significance in the incidence of oral cancer. Methods: The immunohistochemical method and RT-PCR method were applied to detect the expression of COX-2 and MMP-7 in 10 cases with NOM, 33 cases of with OLP and 38 cases with OSCC. Results: The expression of COX-2 mRNA in OSCC tissues (68.4%, 26/38) was significantly higher than in the OLP (24.2%, 8/33) and NOM (0.0%, 0/10) ( P<0.01). The expression of MMP-7 mRNA in OSCC tissues (65.8%, 25/38) was significantly higher than in the OLP (30.3%, 10/33) and NOM (0.0%, 0/10) ( P<0.01). The expression of MMP-7 in OLP was significantly higher than in the NOM ( P<0.05). There was no significant expression of COX-2 protein in NOM, and the positive rate was 42.4% (14/33) and 89.5% (34/38) in OLP and OSCC group, respectively. The COX-2 expression in cancer tissues was significantly higher than in NOM and OLP ( P<0.05). The MMP-7 protein expression in cancer tissues (84.2%, 32/38) was significantly higher than in NOM (10.0%, 1/10) and in OLP (42.4%, 14/33), and the positive rate in OLP was significantly higher than in NOM ( P<0.01). The COX-2 expression was associated with clinical stage ( P<0.05), the MMP-7 expression was associated with clinical stage and lymph node metastasis ( P<0.05). The expressions of COX-2 and MMP-7 mRNA were positively correlated with OSCC. Conclusions: The abnormal expressions of COX-2 and MMP-7 are closely related to the biological behavior of OSCC, the MMP-7 may be induced by COX-2, and further lead to the invasion and metastasis of OSCC.展开更多
Objective To construct Cox7a2 fluorescent vector and study its effect on cytochrome C oxidase ( COX) activity in mouse Sertoli cell line TM4. Methods The coding region of CoxTa2 was amplified from mouse Sertoli cell l...Objective To construct Cox7a2 fluorescent vector and study its effect on cytochrome C oxidase ( COX) activity in mouse Sertoli cell line TM4. Methods The coding region of CoxTa2 was amplified from mouse Sertoli cell line TM4 by RT-PCR. PCR product was展开更多
目的探讨环氧化酶-2(COX-2)及基质金属蛋白酶-7(MMP-7)在胃癌中的表达,关注二者的关系及临床意义。方法采用免疫组化Envision法检测117例胃癌术后存档的标本作为研究对象,以44例正常胃黏膜组织作为对照组,检测两组中COX-2和M M P-7的表...目的探讨环氧化酶-2(COX-2)及基质金属蛋白酶-7(MMP-7)在胃癌中的表达,关注二者的关系及临床意义。方法采用免疫组化Envision法检测117例胃癌术后存档的标本作为研究对象,以44例正常胃黏膜组织作为对照组,检测两组中COX-2和M M P-7的表达。结果胃癌组织中COX-2、M M P-7表达阳性率为59.0%(69/117)和60.7%(71/117),而正常胃组织中均呈低表达或不表达,两者差异有显著性(P<0.01);二者表达均与肿瘤浸润深度、临床分期及淋巴结转移呈明显正相关(P<0.05);相关性研究显示COX-2表达与M M P-7表达呈明显正相关。结论胃癌组织中COX-2、MMP-7异常表达,可促进胃癌的浸润、转移,对判断胃癌预后有一定价值。展开更多
文摘【目的】观察青藤碱(sinomenine,SIN)对环氧化酶2(COX2)、α7烟碱型乙酰胆能受体(α7nAChR)和腺苷受体(A2A)表达变化的影响,探讨青藤碱对A549细胞增殖的抑制作用及机制。【方法】采用四甲基偶氮唑盐微量酶反应比色(MTT)法检测青藤碱和4-甲基亚硝胺基-1-3-吡啶基-1-丁酮(NNK)对A549细胞增殖的影响;细胞划痕实验观察青藤碱和NNK对A549细胞迁移的影响;Western blot法观察青藤碱和NNK对A549细胞COX2蛋白表达的影响;RT-PCR和Western blot法观察青藤碱和NNK对A549细胞α7n ACh R、A2A表达的影响。【结果】NNK能促进增殖和迁移,青藤碱能抑制A549细胞增殖和迁移;NNK组COX2蛋白质水平增加,青藤碱组COX2蛋白质水平下降;NNK组α7n ACh R、A2A的表达增加,青藤碱组α7n ACh R、A2A表达下降。【结论】青藤碱能通过抑制COX2蛋白表达发挥抗A549细胞增殖和迁移作用;青藤碱对α7n ACh R和A2A受体的表达均有抑制作用。
文摘Aim： To investigate the regulatory function of Cox7a2 on steroidogenesis and the mechanism involved in TM3 mouse Leydig cells. Methods： The cDNA of Cox7a2 was cloned from TM3 mouse Leydig cells. It was subcloned to pDsRed- Express-N 1 and transfected back into TM3 mouse Leydig cells for Cox7a2 overexpression by transient gene transfection. Steroidogenesis affected by overexpressed Cox7a2 was studied by ELISA. To elicit the mechanism of this effect, expression of steroidogenic acute regulatory （STAR） protein and reactive oxygen species （ROS） were examined by Western blot and fluorometer, respectively. Results： The cDNA of Cox7a2 （249 bp） was cloned from Leydig cells and confirmed by DNA sequencing. After constructed pDsRed-Express-N1-Cox7a2 was transfected back into TM3 mouse Leydig cells, Cox7a2 inhibited not only luteinizing hormone （LH）-induced secretion of testosterone but also the expression of StAR protein. At the same time, Cox7a2 increased the activity of ROS in TM3 mouse Leydig cells. Conclusion： Cox7a2 inhibited LH-induced StAR protein expression, and consequent testosterone production, at least in part, by increasing ROS activity in TM3 mouse Leydig cells.
基金supported by Jinan Science and Technology Development Plans Grant (No.201121040)
文摘Objective: To observe cyclooxygenase (COX)-2 expression in normal oral mucosa (NOM), oral lichen planus (OLP) and oral squamous cell carcinoma (OSCC) and explore its significance in the incidence of oral cancer. Methods: The immunohistochemical method and RT-PCR method were applied to detect the expression of COX-2 and MMP-7 in 10 cases with NOM, 33 cases of with OLP and 38 cases with OSCC. Results: The expression of COX-2 mRNA in OSCC tissues (68.4%, 26/38) was significantly higher than in the OLP (24.2%, 8/33) and NOM (0.0%, 0/10) ( P<0.01). The expression of MMP-7 mRNA in OSCC tissues (65.8%, 25/38) was significantly higher than in the OLP (30.3%, 10/33) and NOM (0.0%, 0/10) ( P<0.01). The expression of MMP-7 in OLP was significantly higher than in the NOM ( P<0.05). There was no significant expression of COX-2 protein in NOM, and the positive rate was 42.4% (14/33) and 89.5% (34/38) in OLP and OSCC group, respectively. The COX-2 expression in cancer tissues was significantly higher than in NOM and OLP ( P<0.05). The MMP-7 protein expression in cancer tissues (84.2%, 32/38) was significantly higher than in NOM (10.0%, 1/10) and in OLP (42.4%, 14/33), and the positive rate in OLP was significantly higher than in NOM ( P<0.01). The COX-2 expression was associated with clinical stage ( P<0.05), the MMP-7 expression was associated with clinical stage and lymph node metastasis ( P<0.05). The expressions of COX-2 and MMP-7 mRNA were positively correlated with OSCC. Conclusions: The abnormal expressions of COX-2 and MMP-7 are closely related to the biological behavior of OSCC, the MMP-7 may be induced by COX-2, and further lead to the invasion and metastasis of OSCC.
文摘Objective To construct Cox7a2 fluorescent vector and study its effect on cytochrome C oxidase ( COX) activity in mouse Sertoli cell line TM4. Methods The coding region of CoxTa2 was amplified from mouse Sertoli cell line TM4 by RT-PCR. PCR product was
文摘目的探讨环氧化酶-2(COX-2)及基质金属蛋白酶-7(MMP-7)在胃癌中的表达,关注二者的关系及临床意义。方法采用免疫组化Envision法检测117例胃癌术后存档的标本作为研究对象,以44例正常胃黏膜组织作为对照组,检测两组中COX-2和M M P-7的表达。结果胃癌组织中COX-2、M M P-7表达阳性率为59.0%(69/117)和60.7%(71/117),而正常胃组织中均呈低表达或不表达,两者差异有显著性(P<0.01);二者表达均与肿瘤浸润深度、临床分期及淋巴结转移呈明显正相关(P<0.05);相关性研究显示COX-2表达与M M P-7表达呈明显正相关。结论胃癌组织中COX-2、MMP-7异常表达,可促进胃癌的浸润、转移,对判断胃癌预后有一定价值。
