Expression of Coxsackievirus and Adenovirus Receptor in Human Lung Cancer： Possible Clinical Significance

OBJECTIVE To explore the relationship between CAR and the development of human lung cancer, as well as to provide the basis for the clinical treatment of lung cancer using an adenovirus vector-based gene therapy. METHODS CAR expression was assessed immunohisto- chemically in tumoral, paraneoplastic and normal samples from 112 lung cancer patients. At the same time, the mRNA and protein expression of CAR in 32 cases were determined by RT-PCR and Western blot. The relationship between CAR expression and clinicopathologic parameters was statistically analyzed. RESULTS There was no expression of CAR in normal lung tissue but a little in paraneoplastic tissue. The positive rate was 43% in squamous cell carcinoma, and 70% in adenocarcinoma. Both were much significantly higher than that in paraneoplastic tissue. The CAR expression level in adenocarcinoma was higher than that in squamous cell cancer, mRNA expression by RT-PCR and protein expression by Western blot were consistent with immunohistochemistry results. CONCLUSION CAR is overexpressed in human lung cancer, especially in adenocarcinoma. This data offer the reliable basis for adenovirus-mediated gene therapy of lung cancer; more important, CAR may take part in the formation or development of lung cancer; this may be exploitable for the development of antibody-directed therapy in human lung cancer.

Inhibitory Effect of Coxsackie Adenovirus Receptor on Invasion and Metastasis Phenotype of Ovarian Cancer Cell Line SKOV3

Full-length coxsackie adenovirus receptor (CAR) eukaryotic expression plasmid was transfected into an ovarian cell line, SKOV3, and its effect on the change of malignant metastasis phenotype was explored. CAR mRNA and protein expression levels among 4 ovarian cancer cell lines (A2780, SKOV3, SW626, CAOV3) and the positive control 293 (a transformed human embryo kidney cell line) was detected by using semi-quantitative RT-RCR and Western blot and compared. CAR-negative SKOV3 was transfected with the eukaryotic expression plasmid containing a full-length CAR cDNA and mock-vector respectively. The positive clones were screened by G418. The biological behavior changes of positive transfected cells were gauged by colony formation in soft agar assay and cell adhesion assay. Among the cell lines, there were obviously different CAR expression levels. CAR could not be detected in SKOV3. In transfected cell group, CAR expression was enhanced obviously as compared with non-transfected or mock-transfected groups. Cell adhesion in the transfected group was promoted. The number of colony formation was reduced significantly in transfected groups (25.32±8.91) as compared with that in non-transfected group (88.75±13.98) and mock-transfected group (82.53±19.37). Among the 4 ovarian cancer cell lines, CAR expression level was variable. Exogenous CAR expression had a potential role in inhibiting the malignant metastasis phenotype of ovary cancer cells.

Expression of coxsackie and adenovirus receptor in small cell lung cancer

Objective: We explored the expression of coxsackie and adenovirus receptor (CAR) in small cell lung cancer (SCLC) tissue. Methods: CAR expression in 31 SCLC was assessed in formaldehyde-fixed, paraffin-embedded tissue according to the EnVision immunohistochemistry procedure, while 3 samples of surgical specimens of non-malignant lung disease were taken as the negative control. Results: We observed that the expression of CAR was detectable positive in all the 31 cases from the small cell lung cancer tissue, in contrasting that non-malignant lung tissue control. Conclusion: The high expression of CAR appeared in SCLC tissue indicates that it play an important role in of adenovirus vector-based gene therapy in SCLC.

Expression and Significance of Coxsackie and Adenovirus Receptor in Renal-Cell Carcinoma

OBJECTIVE To investigate the expression of Coxsackie and Adenovirus receptor （CAR） in renal-cell carcinoma and the relationship of the CAR to the biological behavior of the carcinomas. METHODS The immunohistochemical SP method was used to detect the expression of Coxsackie and Adenovirus receptor in 48 cases of renal- cell carcinoma and in 12 cases of normal renal tissue 2 cm away from the tumor tissue. RESULTS The positive rates of CAR were 100% in 12 cases of para-tumor normal renal tissue and 35.4% in 48 cases of renal-cell carcinoma respectively. The difference of CAR expression between them was significant （P〈0.05）. The grades of the tumor were as follows： 22 in Grade Ⅰ, 17 in Grade Ⅱ and 9 in Grade Ⅲ with the CAR positive rate being 54.5%, 23.5% and 11.1%, respectively. There was a negative correlation between CAR expression and tumor grading （P〈0.05）. In addition, the number of the cases in stages I to IV were 19, 13, 11 and 5 respectively, with the respective positive rates being 57.9%, 30.8%, 18.2% and 0.0%, i.e. there also was a negative relationship between CAR expression and the stage （P〈0.05）. CONCLUSION CAR expression is down-regulated in renal-cell carcinoma compared with normal tissue. The level of CAR may be a sensitive predictor of differentiation, invasion and metastasis. Loss of CAR expression correlates with the invasive phenotype in our analysis of renal-cell carcinoma.

Expression of Coxsackie and Adenovirurus Receptor and its Significance in Human Lung Cancer

OBJECTIVE To study the relationship between the coxsackie and adenovirus receptor （CAR） and the development of human lung cancer. To optimize adenovirus vector-based gene therapy.METHODS The expression of CAR in 112 cases of lung cancer was examined using immunohistochemistry. At the same time, the relationship between CAR expression and clinicopathologic characteristics was analyzed,RESULTS ：lhere is a little expression of CAR in normal lung tissue. Compared with paraneoplastic epithelial tissue of the lung, the expression of CAR is generally up-regulated in tumor tissues showing a significant dif- ference （P〈0.01）. The positive rate of CAR expression in squamous cell carcinoma was 43.1%, and in adenocarcinoma 70.2%, with the difference between the two rates being statistically significant （P〈0.01）. Compared to the paraneoplastic tissues, the difference in CAR positive expression was 35.4% for squamous cell carcinoma and 38.3% for adenocarcinoma. But the difference in different stages of squamous cell carcinoma had no statistical significance （P〉0.05）. However, the expression of CAR was at a high level in the bronchioalveolar carcinomas as 80.4% were CAR positive. This research showed that there was a specially high expression of CAR in adenocarcinomas.CONCLUSION CAR is expressed in human lungs at a low level and up-regulated in the tumor tissues, suggesting that there is a relationship between adenocarcinoma and CAR. This research provides a basis for planning a regimen of gene therapy using an adenovirus vector,

Down-regulation of Coxsakie and Adenovirus Receptor during Embryo Implantation

In this study,real-time PCR and immunohistochemistry were used to detect coxsakie and adenovirus receptor (CAR) expression.Both localization and quantity were evaluated in the uteri ob-tained at days post coitus (dpc) 2.5,4.5,6.5,8.5.Outcome of PCR was assessed by 2-ΔΔCt method.Im-age Pro-Plus 6.0 software was used for quantifying mean density of CAR expression in immunohisto-chemical sections.We found relatively weak CAR expression in the mouse uteri during implantation window.PCR and immunohistochemistry revealed highest CAR expression was detected on dpc 2.5 followed by down-regulation of CAR at dpc 4.5 and 6.5 (with significant difference).At dpc 8.5,CAR expression was increased slightly again.It is concluded that during implantation,the expression of CAR mRNA and protein is declined,resulting in the impairment of tight junction between cavity epithelium cells.After implantation window closure,CAR appears again to maintain epithelium stability.CAR might play an important role during embryo implantation procedure.

黄芪甲甙对病毒性心肌炎小鼠心肌柯萨奇病毒受体基因表达的影响

目的 探讨黄芪活性成分黄芪甲甙对病毒性心肌炎 (VM)小鼠心肌柯萨奇 腺病毒受体 (CAR)基因表达影响。方法 Balb/c小鼠 1 0 0只 ,分为 6组。非感染小鼠腹腔无菌注射病毒培养液 ,分为正常对照组 (A组 ,1 0只 ,以羧甲基纤维素钠 0 .1mL灌胃 7d)、9%黄芪甲甙对照组 (B组 ,1 0只 ,9%黄芪甲甙 0 .1mL灌胃 7d) ;余 80只小鼠以柯萨奇病毒 (CVB3)腹腔无菌注射制作病毒性心肌炎模型 ,VM小鼠随机分为心肌炎对照组和 1 %、3 %、9%黄芪甲甙干预心肌炎组 ,分别以生理盐水、1 %、3 %、9%黄芪甲甙 0 .1mL灌胃 7d(分别为C、D、E、F组 ,每组 2 0只 )。 1 4d后处死一小部分鼠并取其心脏。采用RT PCR检测心肌CARmRNA表达水平。结果 F组小鼠死亡率较C组明显降低 (1 0 %与 45 %比较 ) ,(χ2 =6 .1 4 P <0 .0 5) ,B组无小鼠死亡 ;与C组相比较 ,CARmRNA表达水平以心肌病变积分在F组显著下降 ,而 1 %、3 %黄芪甲甙对心肌炎小鼠CARmRNA表达及心肌病变积分无明显影响。结论 9%黄芪甲甙可抑制CAR表达 ,提高心肌炎小鼠生存率 ,减轻心肌损害 ,安全有效 ,对VM有良好的治疗作用。

柯萨奇-腺病毒受体基因-968G/A多态性与柯萨奇病毒性心肌炎的关系

目的探讨柯萨奇-腺病毒受体(CAR)基因-968G/A多态性与中国南方汉族人群柯萨奇病毒性心肌炎的关系。方法收集病毒性心肌炎患儿280例,将其中CVB-IgM阳性130例作为心肌炎组,并选择同期130名健康儿童作为对照组,采用聚和酶链反应-限制性内切酶片断长度多态性(PCR-RFLP)、DNA测序等方法检测2组CAR基因-968G/A多态性,采用酶联免疫吸附法(ELISA)测定两组血清CAR水平。结果心肌炎组GG基因型和G等位基因频率均显著高于对照组(P<0.05)。心肌炎组血清CAR水平明显高于对照组(P<0.01),CAR基因-968G/A多态位点中含G等位基因(GG+GA)者血清CAR水平和CVB感染率均显著高于非G等位基因(AA)携带者(P均<0.05)。结论 CAR基因-968G/A多态性可能与中国南方汉族人群柯萨奇病毒性心肌炎有关,G等位基因是柯萨奇病毒性心肌炎的易感基因。

柯萨奇腺病毒受体与病毒性心肌炎

柯萨奇 腺病毒受体是一种普通的存在于心肌细胞表面的病毒受体 ,作为柯萨奇病毒和腺病毒的共同受体 ,在它们感染心肌细胞的过程中起着关键性的作用。

柯萨奇-腺病毒受体在肾癌组织中的表达及意义

目的:研究柯萨奇-腺病毒受体(CAR)在肾癌组织中的表达及意义。方法:应用免疫组化SP法检测12例癌旁正常肾组织和48例肾细胞癌组织中CAR的表达。结果:12例正常肾组织全部表达CAR,48例肾细胞癌组织中31例无CAR表达。不同分级CAR表达率分别为Ⅰ级54.5%(12/22)、Ⅱ级23.5%(4/17)、Ⅲ级11.1%(1/9);不同分期CAR表达率分别为Ⅰ期57.9%(11/19)、Ⅱ期30.8%(4/13)、Ⅲ期18.2%(2/11)、Ⅳ期0(0/5)。结论:在多数肾细胞癌组织中CAR基因表达丧失;CAR表达变化与肾癌的分级、分期相关,可以作为肾癌分化、转移的重要生物学指标。

柯萨奇-腺病毒受体在肿瘤发生发展机制中的研究进展

柯萨奇-腺病毒受体(Coxsackie and adenovirus receptor,CAR)最早作为2型和5型腺病毒的受体而被人们发现和认识。大量研究发现CAR可以影响肿瘤细胞的生长、细胞骨架的变化、细胞间的粘附等,从而在肿瘤侵袭转移过程中起非常重要的作用。而且,CAR的表达与肿瘤的预后和腺病毒介导的减瘤效应也有密切的关系。基于CAR的重要作用,CAR已逐渐成为肿瘤发生发展机制及治疗领域研究中新的"热点",本文结合CAR的基本结构及功能对以上研究的进展作一综述。

柯萨奇-腺病毒受体在病毒性扩张型心肌病外周血白细胞中的表达及意义

目的 观察柯萨奇腺病毒受体 (CAR)在病毒性扩张型心肌病 (DCM )外周血白细胞中的表达及意义。方法 选择柯萨奇B组病毒性DCM及正常人各 15例作为研究对象 ,采用流式细胞术检测外周血白细胞中CAR表达水平 ,并对DCM患者CAR表达值与心功能值 [射血分数 (EF) ]的变化进行相关性分析。结果 流式细胞术检测表明 ,心肌病组平均荧光强度 (MFI)和阳性细胞百分率 (PPC)均明显高于正常对照组 ,两者比较 ,差异均有统计学意义(t =9.39和 7.2 1,P均 <0 .0 1) ,而且其MFI与EF值呈显著负相关 (r =- 0 .6 4 ,P <0 .0 5 )。结论 柯萨奇B组病毒性DCM患者外周血白细胞中CAR表达水平增加 ,外周血白细胞CAR水平的检测 ,有助于DCM的诊断、判断病情和预后。

柯萨奇病毒-腺病毒受体在结肠癌组织中的表达及意义

目的:柯萨奇病毒-腺病毒受体(coxsackie and adenovirus receptor,CAR)介导腺病毒与靶细胞之间的粘附,是腺病毒转染靶细胞的限速因子。本文分析CAR在瘤旁上皮组织、结肠肿瘤原发灶和淋巴结转移灶中的表达,为设计腺病毒载体基因治疗方案提供依据。方法:采用免疫组织化学方法检测62例结肠癌组织中CAR的表达,分析CAR表达与临床病理特征的关系。结果:与瘤旁上皮组织相比,肿瘤组织普遍存在CAR表达下调,但CAR在原发灶和淋巴结转移灶的表达水平无明显差异。CAR的表达水平与患者年龄及肿瘤大小相关。结论:结肠癌组织中存在CAR表达下调,提示在设计腺病毒载体基因治疗方案时应充分考虑CAR表达变化对疗效的影响。

CAR抑制卵巢癌细胞株SKOV3侵袭转移表型的研究

背景与目的:柯萨奇-腺病毒受体(coxsackieandadenovirusreceptor,CAR)最早作为2,5型腺病毒的受体而被人们发现和认识,近年的研究发现它还是粘附分子家族的一员,并参与肿瘤恶性表型改变。本研究通过转染真核表达载体,探讨其对卵巢癌细胞株SKOV3侵袭转移表型的影响。方法:利用Westernblot和RT-PCR检测4株卵巢癌细胞CAR的表达;脂质体介导转染含有全长CAR基因的真核表达质粒至SKOV3细胞,经G418筛选出稳定表达的阳性细胞克隆;体外粘附实验及软琼脂克隆形成实验验证CAR对SKOV3侵袭转移表型的影响。结果:4株卵巢癌细胞中,CAR表达水平不同程度下调,其中SKOV3细胞CAR表达缺失。转染后,SKOV3细胞CARmRNA和蛋白质水平表达均明显增高;细胞间粘附力明显增强;软琼脂克隆实验显示转染细胞每孔克隆形成数(25.3±8.9)明显低于未转染组(88.8±14.0)和转染空质粒组(82.5±19.4)。结论:外源性CAR基因的导入可以增强卵巢癌细胞SKOV3间的粘附性,从而抑制肿瘤细胞的侵袭转移表型。

肿瘤细胞CAR表达水平与5型腺病毒转导效率的关系

目的：通过体外、体内实验探讨肿瘤细胞柯萨奇腺病毒受体（coxsackie adenovirus receptor，CAR）表达水平与腺病毒转导效率的关系，为腺病毒相关的生物制剂在临床个体化应用提供实验依据。方法：将载有绿色荧光蛋白的Ad5型腺病毒（Ad．GFP）以100MOI、200MOI分别感染人食管癌细胞系KYSE510、KYSE150、EC9706、人宫颈癌细胞系HeLa、人卵巢癌细胞系SKOV3、人肝癌细胞系HepG2和人肺癌细胞系A549，感染后48h通过流式细胞术检测Ad—GFP对不同细胞系的转导效率。采用Western blotting方法检测这些细胞中CAR的表达水平。将HeLa、A549、SKOV3、EC9706细胞分别接种于裸鼠腋下，接种细胞数分别为3×10^6、3×10^6、3×10^6、2×10^6，建立裸鼠移植瘤模型。待肿瘤长径达5～7mm时，在裸鼠移植瘤内注射Ad—GFP，每次1×10^9PFU，间隔48h注射第2次。第2次注射后48h处死裸鼠，剖取瘤组织，荧光显微镜观察冰冻切片中GFP的表达情况以判定腺病毒在瘤体内的转导效率，同时用免疫组化法检测瘤组织内的CAR表达水平。结果：200MOIAd—GFP感染A549、HeLa、HepG2、KYSE150细胞48h后分别有92．67％、89．31％、84．98％、74．59％的细胞表达GFP；而SKOV3、KYSE510、EC9706细胞中腺病毒的转导效率明显降低，GFP阳性率分别为30．06％、27．40％、18．93％；各种细胞的CAR蛋白表达水平与腺病毒的转导效率呈正相关。注射Ad—GFP的裸鼠移植瘤组织中可见HeLa、A549瘤组织内有明显的点状绿色荧光，而SKOV3、EC9706瘤组织内表达GFP的细胞数明显少于前两种瘤组织；HeLa、A549裸鼠移植瘤组织内大多数瘤细胞高表达CAR（卅），SKOV3、EC9706移植瘤组织内CAR表达水平较低（＋或-），表明瘤体内CAR表达水平与Ad．GFP的转导效率也呈正相关。结论：体内外实验均显示肿瘤细胞的CAR表达水平与5型腺病毒转导效率密切相关。肿瘤患者治疗前检测组织中CAR表达水平有助于规范腺病毒载体的基因治疗药物的个体化使用。

改良腺病毒AdF35-eGFP转染人及大鼠骨髓间充质干细胞的效率对比研究

目的:比较改良腺病毒AdF35-增强绿荧光蛋白(eGFP)转染人骨髓间充质干细胞(humanbone marrow mesenchymal stem cells,hBMSCs)和大鼠骨髓间充质干细胞(rat BMSCs,rBMSCs)的效率。方法:分别从人体、大鼠骨髓中分离出BMSCs,成骨、成脂诱导培养以鉴定BMSCs。构建含eGFP的AdF35重组腺病毒载体AdF35-eGFP,以不同感染复数(multiplicity of infection,MOI)分别转染BMSCs后,MTT检测AdF35-eGFP对2种BMSCs的毒性作用,荧光显微镜观察eGFP的表达,流式细胞仪检测转染效率,实时定量PCR检测2种BMSCs表达柯萨奇腺病毒受体(CAR)和CD46mRNA的水平。结果:hBMSCs和rBMSCs从骨髓中分离出来后,分别成功诱导分化成骨和成脂。当MOI为1 000 PFU/mL时,AdF35-EGFP对2种BMSCs的活性均有明显抑制作用(P<0.001)。AdF35-eGFP感染hBMSCs 48 h后,荧光显微镜下可见发强烈绿色荧光的细胞,流式细胞仪检测其转染效率可达(84.8±7.1)%;Ad5-eGFP感染rBMSCs后,荧光显微镜下仅见少量发绿色荧光的细胞,48 h转染效率为(3.1±1.1)%。hBMSCs高表达CD46mRNA,低表达CARmRNA;而rBMSCs则高表达CARmRNA,低表达CD46mRNA,2种基因的表达差异有统计学意义(P<0.01)。结论:AdF35可作为理想载体携带目的基因转染hBMSCs,但不适合作为转染rBMSCs的载体。

柯萨奇病毒腺病毒受体的表达对基因工程腺病毒抗瘤活性的影响

背景与目的:腺病毒是研究最多的一种溶瘤病毒,也是基因治疗最常用的一种载体,但作为溶瘤病毒或载体,其治疗恶性肿瘤的疗效很大程度上受到组织器官表达柯萨奇病毒腺病毒受体(Coxsackieandadenovirusreceptor,CAR)的影响。本研究旨在观察CAR表达对腺病毒感染力及临床疗效的影响。方法:收集29例基因工程腺病毒(H101)临床试验患者的病理标本,用免疫组织化学法检测CAR表达,分析CAR表达与临床疗效的关系。体外通过流式细胞仪检测不同肿瘤细胞表面CAR表达,用MTT法测定病毒对不同细胞的抑制率,以观察CAR表达与H101感染力的关系。结果:29例恶性肿瘤患者中,10例有效病例CAR阳性表达者7例(70.0%),19例无效病例CAR阳性表达仅6例(31.6%),CAR表达与临床疗效呈显著性相关(P=0.048)。体外实验结果显示,不同肿瘤细胞株CAR的表达存在差异,其表达量与H101对细胞的感染力(病毒对肿瘤细胞抑制率)呈正相关(r=0.986)。结论:CAR的表达与腺病毒的感染力及临床疗效密切相关,CAR高表达的肿瘤对腺病毒治疗的疗效较低表达者明显为好。

非小细胞肺癌中CAR和CD46的表达及临床意义

目的研究柯萨奇-腺病毒受体(Coxsackie and adenovirus receptor,CAR)及B组腺病毒受体CD46分子在人非小细胞肺癌(Non-small cell lung carcinoma,NSCLC)组织中的表达水平及临床意义。方法免疫组织化学方法检测144例NSCLC组织中CAR、CD46的表达,对两者的表达水平与腺病毒的转染效率以及肺癌发生、发展之间的关系进行分析。结果 144例NSCLC组织中,CAR在肺鳞癌和肺腺癌中CAR的阳性表达率分别为77.5%(31/40)和36.8%(14/38);CD46则分别为58.3%(14/24)和88.1%(37/42);不同病理类型NSCLC组织中,CAR和CD46的表达模式明显不同(P<0.05)。此外,CAR表达与NSCLC临床TNM分期密切相关。30例TNMⅠ期患者中,13例CAR表达阳性(43.3%),48例Ⅱ～Ⅲ期患者中,32例CAR表达阳性(66.7%),Ⅱ～Ⅲ期患者的CAR阳性表达率明显高于Ⅰ期患者(P<0.05)。结论 CAR及CD46在NSCLC中的表达水平明显不同,鳞癌普遍高表达CAR,腺癌则有CD46的高表达,提示依赖不同受体的腺病毒对NSCLC的基因转移效率也有所不同;CAR表达水平可能是判断NSCLC预后的有用指标。

腺病毒载体在小鼠肝脏的分布及其表达规律研究

目的研究腺病毒载体介导的β-半乳糖苷酶(LacZ)报告基因在小鼠肝脏的分布及表达情况,评价腺病毒载体作为基因治疗转导系统的有效性及安全性,为进一步的目的基因导入及表达奠定基础。方法将Balb/cJ小鼠随机分为正常组及暴发性肝炎组,给小鼠经尾静脉注射2×108PFU腺病毒,收集24h、48h、72h、96h、120h和第7天小鼠血清,检测谷丙转氨酶和总胆红素,同时取肝脏、肺脏、心脏、肾脏标本进行X-gal染色,以观察腺病毒载体在各脏器的表达情况。取MHV-3诱导的暴发性肝炎组小鼠24h、48h、72h、96h肝脏,检测腺病毒载体在肝脏的表达情况。结果腺病毒载体主要在小鼠肝脏表达,并于72小时表达最高,在正常小鼠及暴发性肝炎小鼠肝脏中表达效率分别约55.7%和25.7%,之后逐渐消减;正常组小鼠注射腺病毒载体后,肝功能无明显变化。结论腺病毒载体介导的报告基因可在肝脏高效表达且未见明显的肝损害,可作为基因治疗中安全有效的基因传递系统用于治疗肝脏疾病。

柯萨基B组病毒性心肌炎患儿血清TNF-α、IL-8、IL-6和sIL-2R变化

目的：观察柯萨基Ｂ组病毒（ＣｏｘＢ）性心肌炎患儿血清肿瘤坏死因子α（ＴＮＦ－α）、白细胞介素６（ＩＬ－６）、白细胞介素８（ＩＬ－８）和可溶性白细胞介素２受体（ｓＩＬ－２Ｒ）的水平变化，评价ＣｏｘＢ感染时机体细胞因子的应答及其在心肌细胞免疫病理损伤中的意义。方法：检测血清采自２５例病毒性心肌炎患儿，其中１６例血清ＣｏｘＢ抗原和特异性ＩｇＭ抗体检测均阳性（Ｍ１组）；另９例为血清ＣｏｘＢ抗原阴性、特异性ＩｇＭ阳性（Ｍ２组）。１０例正常儿童血清为对照。血清细胞因子含量测定及ＣｏｘＢ抗原、特异性ＩｇＭ抗体检测均采用酶联免疫吸附试验。结果：ＣｏｘＢ病毒性心肌炎患儿血清ＴＮＦ－α为（２９７．４±２６８．０）ｎｇ／Ｌ、ＩＬ－８为（４８０．９±４３５．５）ｎｇ／Ｌ和ＩＬ－６为（２０７．０±２９３．９）ｎｇ／Ｌ，均显著高于正常对照组；然而，ｓＩＬ－２Ｒ为（３９８４．８±１７８１．０）ｎｇ／Ｌ，与正常对照组相比无明显差异。其中Ｍ１组血清ＴＮＦ－α、ＩＬ－６的含量与Ｍ２组相比无显著性差异，但血清ＩＬ－８含量明显高于Ｍ２组。血清ＴＮＦ－α和ＩＬ－８的浓度变化存在明显的正相关。结论：ＣｏｘＢ病毒性心肌炎患儿血清ｓＩＬ－２Ｒ水平基本正常?