A series of novel benzimidazole derivatives was synthesized and their anti-Coxsackie virus B3 （CVB3） activity was evaluated in VERO ceils. Compounds 9 and 10 exhibited better inhibitory activity than those of ribavi...A series of novel benzimidazole derivatives was synthesized and their anti-Coxsackie virus B3 （CVB3） activity was evaluated in VERO ceils. Compounds 9 and 10 exhibited better inhibitory activity than those of ribavirin （RBV） with IC50 values of 5.30 and 1.06 μg/mL, respectively. ？2009 Xian Jin Luo. Published by Elsevier B.V. on behalf of Chinese Chemical Society. All rights reserved.展开更多
Monolayer culture of spontaneously contracting rat heart cells was obtained from newborn SD rats andseeded intO culture plates. 1OOTCID50 of coxsackie virus B3 (CVB3) (Nancy strain )was added as the infectedgroup. The...Monolayer culture of spontaneously contracting rat heart cells was obtained from newborn SD rats andseeded intO culture plates. 1OOTCID50 of coxsackie virus B3 (CVB3) (Nancy strain )was added as the infectedgroup. The dynamics of taurine transport across membrane of normal heart cells and changes after infectionwere examined by using radioactive isotope tracing techniques. The results demonstrated that: (1)two uptake systems of taurine with different affinities and capacities were present in the Plasma membrane of cul-tured rat cardiOmyocytes,the K. of high and low affinity were 9. 5 X 10-5mol/l, and l. 3 X 1 O 2 mol/l,,respectively; (2)taurine transport across membrane was sodium-dependent with a positive linear relationshipbetween amount of taurine influx and extracellular concentration of sOdium;in addition,it was alsO regulat-ed by osmOlarity; (3) fyalanine,a carboxylic analog of taurine,could inhibit the uptake of taurine in dosedependently; (4)taurine influx was decreased in cultured rat cardiomyocytes after infected with CVB3. (5)the lnhibition ratio of taurine transport was enhanced as the infected time prolonged. This experiment pro-vides a basis fOr further investigation of kinetic test of taurine transport and more study should be carriedout to learn the effect of taurine in viral myocarditis.展开更多
Three new glucosylated caffeoylquinic acid isomers (1-3), along with six known compounds, have been isolated from an aqueous extract of the flower buds of Lonicera japonica. Structures of the new compounds were determ...Three new glucosylated caffeoylquinic acid isomers (1-3), along with six known compounds, have been isolated from an aqueous extract of the flower buds of Lonicera japonica. Structures of the new compounds were determined by spectroscopic and chemical methods as (-)-4-O-(4-O-beta-D-glucopyranosylcaffeoyl)quinic acid (-)-3-O-(4-O-beta-D-glucopyranosylcaffeoyl)quinic acid (2), and (-)-5-O-(4-O-beta-D-glucopyranosylcaffeoyl)quinic acid (3), respectively. In the preliminary in vitro assays, two known compounds methyl caffeate and 2'-O-methyladenosine showed inhibitory activity against Coxsackie virus B3 with IC50 values of 3.70 mu mol/L and 6.41 mu mol/L and SI values of 7.8 and 12.1, respectively. (C) 2015 Chinese Pharmaceutical Association and Institute of Materia Medica, Chinese Academy of Medical Sciences. Production and hosting by Elsevier B.V.展开更多
Objective: To study the effect of Huodan tablet (藿丹片, HDT) in treating infantile viral myocarditis. Methods: Clinical manifestations and physical signs as well as laboratory examinations have been observed. Results...Objective: To study the effect of Huodan tablet (藿丹片, HDT) in treating infantile viral myocarditis. Methods: Clinical manifestations and physical signs as well as laboratory examinations have been observed. Results: The markedly effective rate was 68%, and total effective rate 91.67% in the treated group, while in the control group, the markedly effective rate was 30.83%, and the total effective rate 70.84%. According to Ridit analysis, significant difference was shown between the two groups. r-treated group=0.5000, r-control group=0.295+2×0.025 respectively. Conclusion: HDT has no toxic side-effect and can be taken safely and conveniently, it conforms to the demands of WHO on new drug for prevention and cure of myocarditis.展开更多
Three new phenolics（1–3） and twenty-eight known compounds（4–31） were isolated from an ethanolic extract of roots of Alangium chinense. Compound 11 exhibited antiviral activity against Coxsackie virus B3 with IC5...Three new phenolics（1–3） and twenty-eight known compounds（4–31） were isolated from an ethanolic extract of roots of Alangium chinense. Compound 11 exhibited antiviral activity against Coxsackie virus B3 with IC50 values of 16.89 mmol/L. Compounds 1, 10–17, 19–21, and 23 showed strong antioxidant activity against Fe^2＋-cysteine-induced rat liver microsomal lipid peroxidation, with IC50 values of 0.14–8.18 mmol/L.展开更多
文摘A series of novel benzimidazole derivatives was synthesized and their anti-Coxsackie virus B3 （CVB3） activity was evaluated in VERO ceils. Compounds 9 and 10 exhibited better inhibitory activity than those of ribavirin （RBV） with IC50 values of 5.30 and 1.06 μg/mL, respectively. ？2009 Xian Jin Luo. Published by Elsevier B.V. on behalf of Chinese Chemical Society. All rights reserved.
文摘Monolayer culture of spontaneously contracting rat heart cells was obtained from newborn SD rats andseeded intO culture plates. 1OOTCID50 of coxsackie virus B3 (CVB3) (Nancy strain )was added as the infectedgroup. The dynamics of taurine transport across membrane of normal heart cells and changes after infectionwere examined by using radioactive isotope tracing techniques. The results demonstrated that: (1)two uptake systems of taurine with different affinities and capacities were present in the Plasma membrane of cul-tured rat cardiOmyocytes,the K. of high and low affinity were 9. 5 X 10-5mol/l, and l. 3 X 1 O 2 mol/l,,respectively; (2)taurine transport across membrane was sodium-dependent with a positive linear relationshipbetween amount of taurine influx and extracellular concentration of sOdium;in addition,it was alsO regulat-ed by osmOlarity; (3) fyalanine,a carboxylic analog of taurine,could inhibit the uptake of taurine in dosedependently; (4)taurine influx was decreased in cultured rat cardiomyocytes after infected with CVB3. (5)the lnhibition ratio of taurine transport was enhanced as the infected time prolonged. This experiment pro-vides a basis fOr further investigation of kinetic test of taurine transport and more study should be carriedout to learn the effect of taurine in viral myocarditis.
基金Financial support from the National Natural Science Foundation of China (NNSFC,Nos.20772156 and 30825044)the Program for Changjiang Scholars and Innovative Research Team in University (PCSIRT,No.IRT1007)the National Science and Technology Project of China (No.2012ZX09301002-002)
文摘Three new glucosylated caffeoylquinic acid isomers (1-3), along with six known compounds, have been isolated from an aqueous extract of the flower buds of Lonicera japonica. Structures of the new compounds were determined by spectroscopic and chemical methods as (-)-4-O-(4-O-beta-D-glucopyranosylcaffeoyl)quinic acid (-)-3-O-(4-O-beta-D-glucopyranosylcaffeoyl)quinic acid (2), and (-)-5-O-(4-O-beta-D-glucopyranosylcaffeoyl)quinic acid (3), respectively. In the preliminary in vitro assays, two known compounds methyl caffeate and 2'-O-methyladenosine showed inhibitory activity against Coxsackie virus B3 with IC50 values of 3.70 mu mol/L and 6.41 mu mol/L and SI values of 7.8 and 12.1, respectively. (C) 2015 Chinese Pharmaceutical Association and Institute of Materia Medica, Chinese Academy of Medical Sciences. Production and hosting by Elsevier B.V.
文摘Objective: To study the effect of Huodan tablet (藿丹片, HDT) in treating infantile viral myocarditis. Methods: Clinical manifestations and physical signs as well as laboratory examinations have been observed. Results: The markedly effective rate was 68%, and total effective rate 91.67% in the treated group, while in the control group, the markedly effective rate was 30.83%, and the total effective rate 70.84%. According to Ridit analysis, significant difference was shown between the two groups. r-treated group=0.5000, r-control group=0.295+2×0.025 respectively. Conclusion: HDT has no toxic side-effect and can be taken safely and conveniently, it conforms to the demands of WHO on new drug for prevention and cure of myocarditis.
基金supported by the National Science and Technology Project of China (No. 2009ZX09311-004)the National Natural Science Foundation of China (No. 201072234)
文摘Three new phenolics（1–3） and twenty-eight known compounds（4–31） were isolated from an ethanolic extract of roots of Alangium chinense. Compound 11 exhibited antiviral activity against Coxsackie virus B3 with IC50 values of 16.89 mmol/L. Compounds 1, 10–17, 19–21, and 23 showed strong antioxidant activity against Fe^2＋-cysteine-induced rat liver microsomal lipid peroxidation, with IC50 values of 0.14–8.18 mmol/L.
