Differential Expression of Iron Deficiency Responsive Rice Genes under Low Phosphorus and Iron Toxicity Conditions and Association of OsIRO3 with Yield in Acidic Soils

With the hypothesis that iron(Fe) deficiency responsive genes may play a role in Fe toxicity conditions,we studied five such genes OsNAS1,OsNAS3,OsIRO2,OsIRO3 and OsYSL16 across six contrasting rice genotypes for expression under high Fe and low phosphorus(P) conditions,and sequence polymorphism.Genotypes Sahbhagi Dhan,Chakhao Poirieton and Shasharang were high yielders with no bronzing symptom visible under Fe toxic field conditions,and BAM350 and BAM811 were low yielders but did not show bronzing symptoms.Hydroponic screening revealed that the number of crown roots and root length can be traits for consideration for identifying Fe toxicity tolerance in rice genotypes.Fe contents in rice roots and shoots of a high-yielding genotype KMR3 showing leaf bronzing were significantly high.In response to 24 h high Fe stress,the expression levels of OsNAS3 were up-regulated in all genotypes except KMR3.In response to 48 h high Fe stress,the expression levels of OsNAS1 were3-fold higher in tolerant Shasharang,whereas in KMR3,it was significantly down-regulated.Even in response to 7 d excess Fe stress,the transcript abundances of OsIRO2 and OsNAS3 were contrasting in genotypes Shasharang and KMR3.This suggested that the reported Fe deficiency genes had a role in Fe toxicity and that in genotype KMR3 under excess Fe stress,there was disruption of metal homeostasis.Under the 48 h low P conditions,OsIRO2 and OsYSL16 were significantly up-regulated in Fe tolerant genotype Shasharang and in low P tolerant genotype Chakhao Poirieton,respectively.In silico sequence analysis across 3 024 rice genotypes revealed polymorphism for 4 genes.Sequencing across OsIRO3and OsNAS3 revealed nucleotide polymorphism between tolerant and susceptible genotypes for Fe toxicity.Non-synonymous single nucleotide polymorphisms and insertion/deletions(InDels) differing in tolerant and susceptible genotypes were identified.A marker targeting 25-bp InDel in OsIRO3,when run on a diverse panel of 43 rice genotypes and a biparental population,was associated with superior performance for yield under acidic lowland field conditions.This study highlights the potential of one of the vital genes involved in Fe homeostasis as a genic target for improving rice yield in acidic soils.

A New Type of Multielements-Doped,Carbon-based Materials Characterized by High-thermoconductiv-ity,Low Chemical Sputtering,Low RES Yield and Exposure to Plasma

Low-Z materials, such as carbon-based materials and Be, are major plasma-facing material (PFM) for current, even in future fusion devices. In this paper, a new type of multielement-doped carbon-based materials developed are presented along with experimental re-sults of their properties. The results indicate a decrease in chemical sputtering yield by one order of magnitude, a decrease in both thermal shock resistance and radiation-enhanced sublimation, an evidently lower temperature desorption spectrum, and combined properties of exposing to plasma.

The Type of Low-yielding Fields,Using Direction and Land Fertility Building Measures in Suiping County

Using the evaluation indicator system for arable land fertility in Suiping County,this paper analyzes some factors influencing agricultural production,such as physical and chemical properties of soil,site conditions,soil management,and soil nutrients concerning various types of low-yielding fields in the county. In accordance with the dominant soil constraint factors and main direction of improvement,the lowyielding fields in the county are divided into four types: irrigation improvement type,waterlogging drainage type,barren soil fertilization and barrier layer type. Finally this paper offers specific guidance on the construction of arable land.

Biochemical Mechanism Unlocking Their Potential Role in Salt Tolerance Mechanism of Zizyphus Germplasm

Salinity is one of the major constraints reducing plant growth and yield.Irrigation with poor quality and brackish water to orchards is a major cause of stunted growth and low yield.The salt tolerance mechanism is one of the complicated genomic characters that is very problematic to develop in fruit trees and becomes much more severe at any growth and developmental stage.Osmotic stress and hormonal imbalances are major constraints causing low biomass production.Fruit tree tolerance/sensitivity is chiefly based on the activation of a defense system comprised of super-oxidase dismutase(SOD),peroxidase(POD)and catalases(CAT),non-enzymatic compounds including ascorbic acid,phenolics,flavonoids,stress indicators[i.e.,hydrogen peroxide(H2O2),lipid peroxidation,malondialdehyde(MDA),reactive oxygen species(ROS)and osmolytes containing proline,glycine-betaine(GB),ascorbates(APX),glutathione peroxidase(GPX)and glutathione reductase(GR)].Tolerant genotypes must have higher antioxidant assays to cope with the adverse effects of salinity stress because their defense system had the potential to scavenge toxic ROS and protect from membrane leakage.Some work is conducted on agronomic and horticultural crops;however,underutilized fruit crops are still neglected and need serious consideration from plant researchers.Minor fruit crops especially Zizyphus had excellent nutritional aspects.The current study provides detailed insights into the physiological and biochemical mechanisms of Zizyphus species to cope with the adverse effects of salinity by improving their plant defense system.The development of salt-tolerant germplasm is a requisite and can be developed by utilization of physiological,biochemical,and molecular mechanisms.Application of different molecular approaches(i.e.,genome mapping,genome editing,genetic transformation,proteomics,transcriptomics,and metabolites)are effective for higher yield by improving tolerance mechanisms.

Effects of Low Light on Agronomic and Physiological Characteristics of Rice Including Grain Yield and Quality

Light intensity is one of the most important environmental factors that determine the basic characteristics of rice development. However, continuously cloudy weather or rainfall, especially during the grain-filling stage, induces a significant loss in yield and results in poor grain quality. Stress caused by low light often creates severe meteorological disasters in some rice-growing regions worldwide. This review was based on our previous research and related research regarding the effects of low light on rice growth, yield and quality as well as the formation of grain, and mainly reviewed the physiological metabolism of rice plants, including characteristics of photosynthesis, activities of antioxidant enzymes in rice leaves and key enzymes involved in starch synthesis in grains, as well as the translocations of carbohydrate and nitrogen. These characteristics include various grain yield and rice quality components （milling and appearance as well as cooking, eating and nutritional qualities） under different rates of shading imposed at the vegetative or reproductive stages of rice plants. Furthermore, we discussed why grain yield and quality are reduced under the low light environment. Next, we summarized the need for future research that emphasizes methods can effectively improve rice grain yield and quality under low light stress. These research findings can provide a beneficial reference for rice cultivation management and breeding program in low light environments.

Low cycle fatigue estimation of low yield point steel

With the development of technology for earthquake resistant,the research of the low yield point(LYP) steel which used for the fabrication of energy dissipation damper were paid more and more attention.The common studies of the low yield point steel is mainly about the performance with constant amplitude and constant frequency.The low cycle fatigue properties of low yield piont steel were studied by series of test with continuous varying amplitude and varying frequency with the materials testing system by us.The test results showed that low yield point steel of Baosteel have excellent low cycle fatigue properties,which meet the requirement for steel used for the fabrication of energy dissipation damper completely.The low cycle fatigue performance of low yield point steel of Baosteel mainly depended on the amplitude in test.And the effect of varying frequency for the low yield point steel was more less than varying amplitude.

Influence of Climatic Parameters on Soybean Subjected to Low Nitrogen Application Rates in Southern Guinea Savanna of Oyo State, Nigeria

The variability in weather patterns consequent upon climate change constitutes a critical factor influencing soil N availability and the performance of crops. This paper aimed at evaluating the effects of climatic factors on soybean subjected to low N rates under rain-fed situation in the southern Guinea agro-ecology of Oyo State, Nigeria. A 2-year field experiment involving two soybean varieties (TGx1485-1D and TGx1448-2E) and five low rates of N fertilizer application (0, 5, 15, 25, 35 kg/ha) using Urea applied by banding 7 days after planting was arranged in a 5 × 2 split-plot with three replications. N rates constitute the main plot while variety constitutes the sub-plot. Parameters measured include dry shoot weight, shoot N accumulation, and grain yield. Data were subjected to GENSTAT statistical package for analysis, and means separated with Duncan Multiple Range Test (DMRT) at 5% level of probability. Climatic parameters of rainfall, temperature, and potential evapotranspiration data were collected from the surface observatory of the National meteorological agency (NIMET) and subjected to Excel package for computation and graphics. The dry shoot weight increases as N rate increases up to 25 kg/ha, but declines at 35 kg/ha application rate, however, TGx1448-2E produced a higher dry shoot weight (2.9 t/ha) than TGx1485-1D (2.8 t/ha). Low N rates had no significant effects on shoot N accumulation and there was no significant difference in varietal response. Low N rate did not affect grain yield, but the TGx1448-2E had a greater yield of 1.5 t/ha than TGx1485-1D (1.30 t/ha). Annual rainfall amounts were 935.5 mm and1475.8 mm in 2009 and 2010 respectively. Potential evapotranspiration (PET) values were 1676.5 mm and 1676.8 mm in 2009 and 2010 respectively. Temperature values range from 24˚C to 29.8˚C in both years and the mean monthly temperature for 2009 was 26.5˚C and 27.1˚C for 2010. The application of N fertilizer to soybean requires appropriate timing for effective use. Climatic parameters such as rainfall, temperature, and evapotranspiration have dire consideration for fertilizer use and efficiency.

Actuality and Improvement Measures of Mediumand Low-yielding Farmland in Liaoning Province

In order to improve the quality of cultivated land in Liaoning Province in the light of local conditions, medium- and low-yielding farmland in Liaoning Province are divided into various zones according to characteristics of the terrain, and the practical and feasible improvement measures are put forward based on the current situation and main existing problems in the medium- and low-yielding farmland in different areas,

A New Indicator for Global Food Security Assessment: Harvested Area Rather Than Cropland Area

Cropland area has long been used as a key indicator of food security.However,grain yield is not solely controlled by the area of the cropland.Therefore,we proposed a new indicator to assess food security.Results show that from 1992 to 2004,the global cropland area increased by 840200 km^(2)(99.4%),but the grain yield increased only by 310 million t(29.1%);and from 2004 to 2015,the cropland area decreased by 39000 km^(2)(4.64%),but the grain yield increased by 370 million t(70.84%).This result showed that grain yield was not linearly correlated with cropland area,and delimiting the threshold of cropland protection may not guarantee food security.Combined with further correlation analysis,we found that the increase in the global grain yield was more closely related to the harvested area(R^(2)=0.94),which indicated that the harvested area is a more scientific and accurate indicator than cropland area in terms of guaranteeing food security.Therefore,if governments want to ensure the food security,they should choose a new and more accurate indicator:harvested area rather than cropland area.

Optimal management of nitrogen fertilizer in the main rice crop and its carrying-over effect on ratoon rice under mechanized cultivation in Southeast China

This study attempted to clarify the carrying-over effect of different nitrogen treatments applied to the main crop on the crop population growth and yield formation of ratoon rice under mechanized cultivation in Southeast China.Based on the constant total nitrogen application amounts(225.00 kg ha;)in the main crop,an experiment with different ratios of basal and topdressing nitrogen fertilizer(the ratio of basal fertilizer:primary tillering fertilizer:secondary tillering fertilizer:booting fertilizer at 3:1:2:4(N1),3:2:1:4(N2),3:3:0:4(N3),and 4:3:0:3(N4),respectively,and a control without nitrogen treatment(N0))was set up across two consecutive years in field using hybrid rice variety Yongyou 1540 as the test materials.The results showed that the total tiller number and effective tillering percentage increased in the main crop under the N1 treatment,more nitrogen fertilizer applied in late growth stage of the main crop,and its effective tillering percentage of the main crop was the highest at up to 70.18%,which was 9.15%higher than that of conventional fertilization treatment(N4),more nitrogen fertilizer applied in early growth stage of the main crop.The same tendency was observed in leaf area index(LAI)value of the main crop and its subsequent ratoon rice,which were 16.52 and 29.87%higher,respectively,in the N1 treatment than that in the N4 treatment at the full heading stage.The same was true in the case of the transport rates of stem and sheath dry mater and the canopy light interception rates in both the main and its ratoon crops.The transport rate of stem and sheath in main crop rice under N1 treatment increased by 50.57%compared with N4 treatment.The canopy light interception rate of N1 treatment increased by 5.07%compared with N4 treatment at the full heading stage of the ratoon crop.Therefore,the total actual yield was the highest in the main and its ratoon crops under N1 treatment,averaging 17351.23 kg ha;in two-year trials,which was 23.00%higher than that in the conventional fertilization treatment(N4).The results showed that appropriate nitrogen treatment was able to produce a good crop stand in the main crop,which was essential for producing a good ratoon crop population and high yield especially under mechanized cultivation with low stubble height of the main crop.The study suggested that shifting the proper nitrogen application amounts to the late growth stage of the main crop,such as N1 treatment,not only had a higher productive effect on ensuring the yield of the main crop,but also had a positive effect on the axillary bud sprouts from the stubbles for ratoon rice,resulting in an increased percentage of productive panicles and achieving the goal of one planting with two good harvests under the conditions of our study.

Role of ICSI in Non-male Factor Cycles as the Number of Oocytes Retrieved Decreases from Four to One

This study aimed to investigate whether intracytoplasmic sperm injection（ICSI） shows an advantage over in vitro fertilization（IVF） in non-male factor cycles as the number of oocytes retrieved decreases from four to one.We undertook a retrospective analysis of 1305 IVF/ICSI cycles of non-male factor in which four or fewer oocytes were retrieved.Comparisons were made between conventional IVF（CI） and ICSI when one,two,three or four oocyte（s） were retrieved.Primary outcomes including normal fertilization rate,proportion of embryos per obtained oocyte,cycle cancellation rate,implantation rate,clinical pregnancy rate（PR）,live birth rate（LBR）,cumulative PR and cumulative LBR were evaluated.The results showed that the normal fertilization rate（72.5% vs.50.0%） and the proportion of embryos per obtained oocyte（72.5% vs.55.0%） were significantly increased in one oocyte retrieved cycles in ICSI group as compared with CI group.However,the proportion of embryos per obtained oocyte was markedly decreased in ICSI group when three（52.3% vs.61.3%） or four（56.9% vs.64.0%） oocytes were retrieved.The implantation rates,clinical PRs,LBRs,cumulative PRs and cumulative LBRs in CI group were comparable to those in ICSI group when one,two,three or four oocyte（s） were retrieved.In conclusion,ICSI doesn＇t show advantages over IVF in low oocyte yield cycles of non-male factors,even when only one oocyte was retrieved.Key words

Material Mechanical Properties Necessary for the Structural Intervention of Concrete Structures

Structural intervention involves the restoration and/or upgrading of the mechanical performances of structures. In addition to concrete and steel, which are typical materials for concrete structures, various ber-reinforced polymers (FRPs), cementitious materials with bers, polymers, and adhesives are often applied for structural intervention. In order to predict structural performance, it is necessary to develop a generic method that is applicable to not only to steel, but also to other materials. Such a generic model could provide information on the mechanical properties required to improve the structural performance. External bonding, which is a typical scheme for structural intervention, is not applied for new structures. It is necessary to clarify material properties and structural details in order to achieve better bonding strength at the interface between the substrate concrete and an externally bonded material. This paper presents the mechanical properties of substrate concrete and relevant intervention material for the fol- lowing purposes: ① to achieve better shear strength and ultimate deformation of a member after struc- tural intervention;and ② to achieve better debonding strength for external bonding. This paper concludes that some of the mechanical properties and structural details for intervention materials that are necessary for improvement in mechanical performance in structures with structural intervention are new, and differ from those of structures without intervention. For example, high strength and stiff- ness are important properties for materials in structures without structural intervention, whereas high fracturing strain and low stiffness are important properties for structural intervention materials.

Development of Moisture and Ash Based Correlation for the Estimation of Mineral Matter in High Ash Indian Coal

The experimental determination of mineral matter in coal is a tedious as well as time consuming process, and requires highly skilled analyst to carry out the chemical analysis of coal and ash. On the other hand, determination of moisture and ash is relatively easy using moisture oven and muffle furnace and can be determined anywhere with little care. Most of the methods reported till date for the evaluation of mineral matter in coal involves indirect determination of mineral matter using high temperature ash as one parameter. All these methods offer conversion of ash yield into mineral matter with the help of correlations based on certain assumptions. Although, the method for direct determination of mineral matter by chemical analysis is also reported, but is of little use as it requires extensive chemical analysis. In this study mineral matter is determined directly by oxygen plasma asher in coal samples collected from different regions of India. The results obtained were compared with those obtained by existing correlations. It has been observed that all the existing correlations including Parr’s, are found to be unrealistic for high ash Indian coal. An attempt has been made to develop a new correlation for the estimation of mineral matter based on two simple parameter i.e. moisture content and ash yield. Present model was developed after analyzing 75 coal samples. The developed correlation appears to be more simple and better and is represented as Mineral Matter(MM)= 0.74(Moisture) + 1.17(Ash)

Research advances of SAR remote sensing for agriculture applications: A review

Synthetic aperture radar(SAR) is an effective and important technique in monitoring crop and other agricultural targets because its quality does not depend on weather conditions. SAR is sensitive to the geometrical structures and dielectric properties of the targets and has a certain penetration ability to some agricultural targets. The capabilities of SAR for agriculture applications can be organized into three main categories: crop identification and crop planting area statistics, crop and cropland parameter extraction, and crop yield estimation. According to the above concepts, this paper systematically analyses the recent progresses, existing problems and future directions in SAR agricultural remote sensing. In recent years, with the remarkable progresses in SAR remote sensing systems, the available SAR data sources have been greatly enriched. The accuracies of the crop classification and parameter extraction by SAR data have been improved progressively. But the development of modern agriculture has put forwarded higher requirements for SAR remote sensing. For instance, the spatial resolution and revisiting cycle of the SAR sensors, the accuracy of crop classification, the whole phenological period monitoring of crop growth status, the soil moisture inversion under the condition of high vegetation coverage, the integrations of SAR remote sensing retrieval information with hydrological models and/or crop growth models, and so on, still need to be improved. In the future, the joint use of optical and SAR remote sensing data, the application of multi-band multi-dimensional SAR, the precise and high efficient modeling of electromagnetic scattering and parameter extraction of crop and farmland composite scene, the development of light and small SAR systems like those onboard unmanned aerial vehicles and their applications will be active research areas in agriculture remote sensing. This paper concludes that SAR remote sensing has great potential and will play a more significant role in the various fields of agricultural remote sensing.

Growth traits and nitrogen assimilation-associated physiological parameters of wheat(Triticum aestivum L.) under low and high N conditions

In this study, 14 wheat cultivars with contrasting yield and N use efficiency （NUE） were used to investigate the agronomic and NUE-related traits, and the N assimilation-associated enzyme activities under low and high N conditions. Under deficient-N, the cultivars with high N uptake efficiency （UpE） and high N utilization efficiency （UtE） exhibited higher plant biomass, yields, and N contents than those with medium and low NUEs. The high UpE cultivars accumulated more N than other NUE type cultivars. Under sufficient-N, the tested cultivars showed similar patterns in biomass, yield, and N content to those under deficient-N, but the varietal variations in above traits were smaller. In addition, the high UpE cultivars displayed much more of root biomass and larger of root length, surface area, and volume than other NUE type cultivars, indicating that the root morphological traits under N deprivation are closely associated with the plant biomass through its improvement of the N acquisition. The high UtE cultivars showed higher activities of nitrate reductase （NR）, nitrite reductase （NIR）, and gluta- mine synthetase （GS） at stages of seediling, heading and filling than other NUE type cultivars under both low and high N conditions. Moreover, the high UpE and UtE cultivars also displayed higher photosynthetic rate under deficient-N than the medium and low NUE cultivars. Together, our results indicated that the tested wheat cultivars possess dramatically genetic variations in biomass, yield, and NUE. The root morphological traits and the N assimilation enzymatic acitivities play critical roles in regulating N accumulation and internal N translocation under the N-starvation stress, respectively. They can be used as morphological and biochemical references for evaluation of UpE and UtE in wheat.

Seed priming improves early seedling vigor, growth and productivity of spring maize

Potential of seed priming treatments in improving the performance of early planted maize was evaluated against timely planting. Seeds of maize hybrid FH-810 were soaked in water (hydropriming), CaCl2 (2.2%, osmopriming), moringa leaf extracts (MLE 3.3%, osmopriming) and salicylic acid (SA, 50 mg L–1, hormonal priming) each for 18 h. Untreated and hydroprimed seeds were taken as control. Seeds primed with SA took less time in emergence and had high vigor in early planted maize. Amongst treatments, hormonal priming, reduced the electrical conductivity, increased the leaf relative and chlorophyl contents fol owed by osmopriming with CaCl2 at seedling stage. Likewise, plant height, grain rows and 1 000-grain weight, grain and biological yield and harvest index were also improved by seed priming;however hormonal priming and osmopriming with MLE were more effective in this regard. Improved yield performance by hormonal priming or osmopriming with MLE in early planting primarily owed to increased leaf area index, crop growth and net assimilation rates, and maintenance of green leaf area at maturity. In conclusion, osmopriming with MLE and hormonal priming with SA were the most economical treatments in improving productivity of early planted spring maize through stimulation of early seedling growth at low temperature.

Variability, heritability and genetic advance in mulberry (<i>Morus</i>spp.) for growth and yield attributes

Genetic improvement of crop plants is brought about by manipulating the genetic makeup through systematic breeding techniques or by employing modern biotechnological tools. Application of systematic breeding technique to a large extent is decided by the knowledge on the genetic control of the traits. Keeping this in view, nine mulberry genotypes were evaluated for different growth and yield attributing traits viz., number of tillers (NT), plant height (PH), total shoot length (TSL), nodal distance (ND), leaf fall % (LF), number of leaves/plant (NLP), weight of 100 fresh leaves (WFL), weight of 100 dry leaves (WDL), single leaf area (LA), leaf area index (LAI), aboveground biomass (AGB), leaf harvest index (LHI) and leaf yield (LY) and estimated the magnitude of genotypic and phenotypic variation, heritability, genetic advance and correlation coefficients. The broad sense heritability for these traits ranged from 63.942 (WFL) to 13.261 (PH). High heritability coupled with high genetic advance was recorded for the characters WFL, LF, LA, WDL and LY suggesting the higher genetic control over these traits. Leaf yield showed significantly positive phenotypic and genotypic correlations with all other growth traits except PH and LF. Leaf fall had significant negative correlations with all the highly heritable yield attributes viz., ND (-0.379), WDL (-0.225), LA (-0.346), LAI (-0.233) at 1% level and AGB (-0.148), LHI (-0.122) and LY (-0.146) at 5% level. Likewise, it showed positive correlations with TSL (0.558), NLP (0.264) and PH (0.221). Since mulberry is mainly cultivated for leaf yield, genotypes having higher WFL, LA, WDL and LY and less LF must be given importance during parent selection to evolve high yielding varieties with less leaf fall across different seasons in mulberry.

火灾下LYP160屈曲约束支撑抗震性能研究

为研究屈曲约束支撑(buckling-restrained brace,BRB)及其体系在高温工况的抗震性能,对BRB芯材低屈服点LYP160钢材分别进行了常温和高温材料力学性能试验.通过与试验结果比较,建立了BRB热-力耦合精细化有限元分析模型,验证了该模型的准确性;对火灾下LYP160屈曲约束支撑的温度场、滞回曲线、骨架曲线和耗能能力等性能进行了对比分析;对不同工况(3种火灾工况和常温工况)下8层配置屈曲约束支撑钢框架(BRB structure,BS)和原结构(original structure,OS)分别进行了弹塑性动力时程分析.研究结果表明:3种火灾工况下BS的平均层间位移角较于OS分别降低了30.4%、33.2%和42%;OS柱塑性铰损坏严重,且更多出现在柱上,BS整体损坏程度较OS轻,更为安全可靠.

Apparent quantum yield of photosynthesis of winter wheat and its response to temperature and intercellular CO_2 concentration under low atmospheric pressure on Tibetan Plateau

The Tibetan Plateau is characterized by lower atmospheric pressure, lower air temperature and high daily and seasonal variation due to high elevation. The photosynthesis of plants is significantly influenced by these alpine environmental factors. Apparent quantum yield (αA) is one of the basic parameters of photosynthesis and mass production. Its accuracy determination is of significance to model photosynthesis of C3 plants and global change on the plateau. In the Lhasa Plateau Ecological Station with 65.4 kPa of atmospheric pressure at an elevation of 3688 m, Li-Cor 6400 portable photosynthesis system was used to measure light response curves of winter wheat in different temperatures and intercellular CO2 concentration (Ci).The slope of light response curve in weak light area of PFD from 0 to 150 μmol m-2 S-1 was used to evaluate the value of αA. The dependence of αA on temperature and intercellular concentration was analyzed. In 30℃, the average value of αAWaS 0.0476 ± 0.0038. It is not quite different from the values in low elevation areas. αA is influenced both by temperature and by the ratio of CO2and O2 partial pressure ([CO2]/[O2]). The measured values in the previous study were much lower.This might be due to systematic errors from instrument and data processing methods. The values of αA decreased linearly with temperature. It decreased 0.0007 in every 1℃ increase of temperature. The decrease slope is similar to those of C3 plants in the previous researches. While [O2] is constant, αA increases with Ciwith a hyperbolic relationship. In comparison with low elevation areas, the αA on the Tibetan Plateau is more sensitive to increase of CO2.