Safety,immunogenicity,and cross-species protection of a plasmid DNA encoding Plasmodium falciparum SERA5 polypeptide,microbial epitopes and chemokine genes in mice and olive baboons

Incorporation of biomolecular epitopes to malarial antigens should be explored in the development of straintranscending malarial vaccines.The present study sought to determine safety,immunogenicity and cross-species efficacy of Plasmodium falciparum serine repeat antigen 5 polypeptide co-expressed with epitopes of BacilleCalmette Guerin（BCG）,tetanus toxoid（TT） and a chemokine gene.Olive baboons and BALB/c mice were randomly assigned into vaccine and control groups.The vaccine group animals were primed and boosted twice with pIRES plasmids encoding the SERA5 ＋ BCG ＋ TT alone,or with either CCL5 or CCL20 and the control group with pIRES plasmid vector backbone.Mice and baboons were challenged with P.berghei ANKA and P.knowlesi H strain parasites,respectively.Safety was determined by observing for injection sites reactogenicities,hematology and clinical chemistry.Parasitaemia and survivorship profiles were used to determine cross-species efficacy,and T cell phenotypes,Th1-,Th2-type,T-regulatory immune responses and antibody responses were assessed to determine vaccine immunogenicity.The pSeBCGTT plasmid DNA vaccines were safe and induced Thl-,Th2-type,and Tregulatory responses vaccinated animals showed enhanced CD4~＋（P〈0.01）,CD 8~＋ T cells（P〈 0.001） activation and IgG anti-SE36 antibodies responses（P〈 0.001） at week 4 and 8 post vaccination compared to the control group.Vaccinated mice had a 31.45-68.69%cumulative parasite load reduction and 60%suppression in baboons（P〈0.05）and enhanced survivorship（P〈 0.001） with no clinical signs of malaria compared to the control group.The results showed that the vaccines were safe,immunogenic and conferred partial cross-species protection.

Comparison of Thermo-Structural Responses for Integrated Thermal Protection Panels with Different Corrugated Core Configurations

To explore new light-weight integrated thermal protection system panel configuration and gain good insight into the responses mechanism,heat transfer and structural field analysis for one single-layer and four double-layer corrugated core panels were performed. The obtained the temperature,buckling,stress and deflection responses were compared,and the deflection and stress distributions as well as thermal buckling mode at the time were discussed for the considered configurations when the temperature difference between the top and bottom face sheet was maximum. The results demonstrated that the non-orthogonal and hat-stiffened double-layer structures provide superior performance to resist thermal buckling deformation in comparison with other configurations. The useful information is provided for the forthcoming optimization in which thermal buckling is considered as critical design driver.

交联聚苯乙烯-植物联合护坡技术对黄土陡坡的控蚀效果

随着经济社会的发展,各类基础设施建设使黄土高原地区产生了许多高陡边坡,为了给黄土陡坡的修复及土壤侵蚀控制提供参考,基于野外原位小区观测及人工模拟降雨试验结果,分析了新型有机材料交联聚苯乙烯(CLPs)与植物联合护坡技术对黄土陡坡的控蚀效果及机理等,结果表明:1)采用CLPs对黄土坡面进行处理后,在相同的降雨情况下,其径流深比裸露坡面的大、产沙量与裸露坡面的相当,即仅采用CLPs对黄土坡面进行处理基本无控蚀作用;2)采用CLPs-植物联合护坡技术的CLPs-草灌坡面与CLPs-草皮坡面,径流深远小于传统草灌坡面和鱼鳞坑植灌木坡面的,土壤侵蚀模数远远小于传统草灌坡面和鱼鳞坑植灌木坡面的,年均土壤侵蚀模数仅为传统草灌坡面和鱼鳞坑植灌木坡面的0.9%~3.2%,即CLPs与植物联合对黄土坡面进行防护的控蚀效果显著;3)植被覆盖度为100%的CLPs-草灌联合修复坡面与无植被覆盖的CLPs护坡坡面相比,坡面径流的流速、雷诺数、弗劳德数、剪切力、功率显著减小,坡面阻力系数、糙率系数显著增大,这是CLPs-草灌联合护坡技术控蚀的水动力学机理;4)采用CLPs-植物联合护坡技术,可使黄土陡坡形成有效的土体加固体系、控蚀体系和保水体系。

Effects of natural cerebrolysin on protective proteins and pro-apoptotic molecules in mesenchymal stem cells following beta-amyloid peptide1-40-induced endoplasmic reticulum stress

BACKGROUND： Studies have demonstrated that β-amyloid peptide （Aβ）, a characteristic pathological product of Alzheimer＇s disease （AD）, results in neuronal endoplasmic reticulum stress （ERS）. However, the mechanisms of traditional Chinese medicine against ERS in AD are poorly understood. OBJECTIVE： To measure expression levels of protective proteins （GRP78 and GRP94） of ER molecular partners and pro-apoptotic Caspase-12 ER membrane expression following application of traditional Chinese medicine natural cerebrolysin （NC） to treat Aβ1-40-induced ERS. DESIGN, TIME AND SETTING： A parallel-controlled study was performed at the Institute of Integrated Western and Traditional Chinese Medicine, Shenzhen Hospital of Southern Medical University between September 2006 and November 2008. MATERIALS： Sprague Dawley male rats, 6-8 weeks old, were used to harvest tibial and femoral bone marrow. Isolation and purification of mesenchymal stem cells （MSCs） were established from the whole bone marrow by removing non-adherent cells in primary and passage cultures. Aβ1-40 was provided by Sigma, USA. NC was provided by Shenzhen Institute of Integrated Chinese and Western Medicine, China. NC was predominantly composed of Renshen （Radix Ginseng）, Tianma （Rhizoma Gastrodiae）, and Yinxingye （Ginkgo Leaf） in a proportion of 1 ： 2： 2. Following conventional water extraction technology, an extract （1 ： 20） was prepared. Six adult, male, New Zealand rabbits underwent intragastric administration of NC extract （0.976 g/kg per day） for 1 month to prepare NC-positive serum, and the remaining 6 rabbits received intragastric administration of physiological saline to prepare normal blank serum. METHODS： A total of 500 nmol/L Aβ1-40 was used to establish ERS models of primary cultured MSCs. AD cell models were incubated with different doses of NC-positive serum （2.5%, 5%, and 10%）. MSCs treated with normal blank serum served as normal blank controls. MAIN OUTCOME MEASURES： Reverse transcription-polymerase chain reaction and fluorescent immunocytochemistry were respectively used to measure mRNA and protein expression levels of GRP78, GRP94, and Caspase-12 in MSCs. RESULTS： Following Aβ1-40 exposure, mRNA and protein expression levels of GRP78 and GRP94, as well as Caspase-12, significantly increased （P 〈 0.05）, suggesting successful establishment of ERS models. Following NC-positive serum application, mRNA and protein expression levels of GRP78 and GRP94 in MSCs significantly increased （P 〈 0.05 or P 〈 0.01）. However, mRNA and protein expression levels of Caspase-12 significantly decreased （P 〈 0.05, or P 〈 0.01） compared with the ERS model group. These effects were dose-dependent. CONCLUSION： NC downregulated Caspase-12 expression and upregulated GRP78 and GRP94 expression in MSCs in a dose-dependent manner under the state of Aβ1-40-induced ERS.

A NEW POLYMER-BOUND 1,2-DIOL AS A PROTECTING AGENT FOR SYMMETRICAL DIALDEHYDE

A novel polymer-bound 1,2-diol, 3-polystyrylsulfonyl-1,2-propanediol (6) had been prepared by the reaction of sodium polystyrylsulfinate with allyl bromide, followed by oxidation and. hydrolysis or directly with 3-chloro-1,2-propanediol in the presence of a phase transfer catalyst, n-tetrabutylammonium iodide. The capacity of resin 6 for terephthaidehyde reached 1.43 mmol/g. The aldehydic groups attached to polymer 6 reacted with hydroxylamine hydrochloride or reduced by sodium borohydride giving p-formylbenzaldoxime (yield: 89%)and p-formyl-benzalcohol (yield: 734%), respectively. The high yields of these polymer-supported reactions showed that the polymer 6 possessed the effective isolation of its reactive sites.

Regulating Crossing Border E-commerce by SC

Crossing border e‐commerce develops rapidly because of e‐commerce development.There are three typical modes of crossing border e‐commerce,and they are B2B(business to business),B2C(business to customer),and C2C(customer to customer).With the development of social network,a new mode called C2C cross‐border e‐commerce by SC(social commerce)is occurring.This new kind of e‐commerce has several advantages,like high trust between seller and customer and personalized consumption for customers.But there are still some problems of C2C cross‐border e‐commerce by SC,which are tax evasion and no rule to regulate.To solve these problems,several measures should be taken into account.In the area of Customs Law,it should refine the standard of luggage and post package taxation.The Custom should strengthen supervision for international post package.To deal with the problem that there is no law to regulate C2C cross‐border e‐commerce by SC,Contract Law is suitable before the E‐commerce Law enacts.

Variance Reduction Technique for Estimating Value-at-Risk based on the Cross - Entropy

Value-at-Risk （VaR） estimation via Monte Carlo （MC） simulation is studied here. The variance reduction technique is proposed in order to speed up MC algorithm. The algorithm for estimating the probability of high portfolio losses （more general risk measure） based on the Cross - Entropy importance sampling is developed. This algorithm can easily be applied in any light- or heavy-tailed case without an extra adaptation. Besides, it does not loose in the performance in comparison to other known methods. A numerical study in both cases is performed and the variance reduction rate is compared with other known methods. The problem of VaR estimation using procedures for estimating the probability of high portfolio losses is also discussed.

交错扩散对具有保护区和Allee效应的捕食者-食饵模型的影响

研究了具有保护区域和Allee效应的交错扩散捕食者-食饵模型的稳态问题。运用最大值原理得到稳态解的先验估计,利用分歧理论证明了该系统发自半平凡解的分歧正解的存在性,并讨论了Allee效应常数和扩散系数对该系统的正稳态解的极限行为的影响。

Development and evaluation of neutralizing antibodies for cross-protection against West Nile virus and Japanese encephalitis virus

Background:West Nile virus is a severe zoonotic pathogen that can cause severe central nervous system symptoms in humans and horses,and is fatal for birds,chickens and other poultry.With no specific drugs or vaccines available,antibody-based therapy is a promising treatment.This study aims to develop neutralizing antibodies against West Nile virus and assess their cross-protective potential against Japanese encephalitis virus.Methods:Monoclonal antibodies against WNV and JEV were isolated by hybridoma technology.The therapeutic efficacy of these antibodies was evaluated using a mouse model,and a humanized version of the monoclonal antibody was generated for potential human application.Results:In this study,we generated eight monoclonal antibodies that exhibit neutralizing activity against WNV.Their therapeutic effects against WNV were validated both in vivo and in vitro.Among these antibodies,C9-G11-F3 also exhibited cross-protective activity against JEV.We also humanized the antibody to ensure that it could be used for WNV infection treatment in humans.Conclusion:This study highlights the importance of neutralizing antibodies as a promising approach for pro-tection against West Nile virus infection and suggests their potential utility in the development of therapeutic interventions.

Investor Protection,Ownership Structure and Corporate Valuation

The purpose of this paper is to investigate the relationship between investor protection, ownership structure and corporate valuation. La Porta showed that there existed a simple linear relationship between corporate valuation and the holding percentage of controlling shareholders. But recent empirical evidence does not support it. A nonlinear relationship is proved between ownership structure and corporate valuaton by relaxing the assumption of La Porta＇s model in this paper. There exists a positive relation between investor protection and corporate valuation. Our empirical research shows that this relation is significantly positive indeed.

The effect of protective genital care protocol on preventing diaper dermatit development in 0 - 18 month old children using antibiotics

Diaper dermatit is among the widely seen inflamatory skin diseases in neonatals and small 0 - 18 month old children. Control of causative agents as well as providing good genital care may prevent or reduce the risk of the disease. The aim of this study is find out the effect of protective genital care on reducing the diaper dermatit development in 0 - 18 months old inpatient 0 - 18 month old children who are using antibiotic. The study was conducted as prospective half-experimetal using control-experimental groups. Our samples comprise of 0 - 18 month old children admitted at Dokuz Eylul University Practical and Research Hospital, pediatric clinic between January and May 2009, 41 0 - 18 month old children as a control group were not under protective genital care, and 42 0 - 18 month old children as an experimental group were under the protecion and using vaseline. The mothers of the experimental group 0 - 18 month old children were given education. All 0 - 18 month old children were followed-up 3 - 7 days and the collected data were evaluated in computer using Pearson’s chisquare, Fisher’s exact chi-square and Yatest-corrected chi-square tests. The diaper dermatit develop- ment ratio was 34.1% in the control group and 7.1% in the experimental group

First assessment of Noise-Equivalent Sigma-Zero in GF3-02 TOPSAR mode with sea surface wind speed retrieval

Gaofen-3-02(GF3-02)is the first C-band synthetic aperture radar(SAR)satellite with terrain observation with progressive scans of SAR(TOPSAR)imaging mode in China,which plays an essential role in marine environment monitoring.Given the weak scattering characteristics of the ocean,the system thermal noise superimposed on SAR images has significant interference,especially in cross-polarization channels.Noise-Equivalent Sigma-Zero(NESZ)is a measure of the sensitivity of the radar to areas of low backscatter.The NESZ is defined to be the scattering cross-section coefficient of an area which contributes a mean level in the image equal to the signal-independent additive noise level.For TOPSAR,NESZ exhibits the shape of the SAR scanning gain curve in the azimuth and the shape of the antenna pattern in the range.Therefore,the accurate measurement of NESZ plays a vital role in the application of spaceborne SAR sea surface cross-polarization data.This paper proposes a theoretical calculation method for the NESZ curve in GF3-02 TOPSAR mode based on SAR noise inner calibration data and the imaging algorithm.A method for correcting the error existing in the theoretical curve of NESZ is also proposed according to the relationship between sea surface backscattering and wind speed and the same characteristics of target scattering in the overlapping area of adjacent sub-swaths.According to assessment with wide-swath TOPSAR cross-polarization data,the GF3-02 TOPSAR mode has a very low thermal noise level,which is better than−33 dB at the edge of each beam,and controlled below−38 dB at the center of the beam.The two-dimensional reference curves of the NESZ of each beam are provided to the GF3-02 TOPSAR users.After discussing the relationship between normalized radar cross section(NRCS)and wind speed,we provide a formula for NRCS related to wind speed and radar incidence angle.Compared with the NRCS derived from this formula and the NESZ-subtracted NRCS of SAR images,the bias is−0.0048 dB,the Root Mean Square Error is 1.671 dB and the correlation coefficient is 0.939.

论中国数据跨境制度的现状、问题与纾困路径

中国已经建立了以“安全评估、标准合同、保护认证”为核心、以行业规定为配套的数据跨境制度体系,形成了既保安全又促发展的中国方案。但在具体立法中,该制度在理论与实践上存在双重失衡,在理论上缺乏完善的基础理论支撑,在实践中未能形成系统完整的制度体系,且其在适用关系上出现龃龉,与国际规则之间也存在割裂导致难以衔接,致使数据处理者在合规实践中面临较大成本,难以获得预期成效。不同于美国的“市场话语”和欧盟“权利本位”的数据跨境理论基础,中国应当基于国情明确利益平衡体系下的数据主权理论建构数据出境制度体系,矫正实践中过度保障安全的规制思路,通过单列安全评估、制定行业性与地方性特殊制度等措施,完善相关制度之间、国内法与国际规则之间的适用衔接,着力降低合规成本,保障制度落到实处。

能源区块链的跨链服务安全技术研究进展

在“双碳”目标推动下,能源产业数字化转型势在必行.随着区块链在能源行业数字化转型应用和发展,能源区块链概念逐渐形成共识,它是区块链与能源互联网深度融合的产业新形态,可助力能源主体之间的高效协作,为绿色低碳等创新业务模式提供技术支撑与服务.能源区块链的规模化发展离不开多层次跨链技术的突破,但能源区块链的跨链服务还面临着许多问题.将目前能源区块链领域的研究现状分为5类,即能源区块链架构、智能合约应用、跨链技术、区块链节点管理和区块链隐私保护,针对这5个方向分别总结相关研究工作,详细梳理出各研究方案的原理、优势与不足;然后,为促进能源区块链的跨链服务安全技术的发展,根据能源区块链的现实需求,结合监管机制与共识机制提出多层次跨链协同监管的能源区块链架构;最后,总结出能源区块链跨链服务安全技术中亟待解决的问题,并提出区块链在能源领域的研究展望.

广西H9N2亚型禽流感病毒疫苗候选株的筛选

【目的】筛选出免疫原性更优的H9N2亚型禽流感病毒(AIV)疫苗候选株用于新型疫苗研发,为加强对H9N2亚型AIV的防控提供技术支持。【方法】以2000—2020年广西地区分离获得的36株H9N2亚型AIV分离株为试验材料,依据HA基因遗传进化分析选择12株不同年份、不同地域及不同宿主来源的H9N2亚型AIV代表株,与目前使用的商品化H9亚型AIV疫苗株(SS株)进行交叉血凝抑制试验(HI)及抗原性分析,根据抗原分析结果选择其中具有不同抗原差异的代表株制备油乳剂灭活疫苗,并分别与商品化H9亚型AIV疫苗(SS株)进行交叉免疫保护试验。【结果】在36株H9N2亚型AIV广西分离株中有31株属于Y280-Like(9.4.1),其中26株属于分支I、5株属于分支II,另外5株属于G1-Like(h9.4.1),与我国常用疫苗株分属于不同进化分支。12株H9N2亚型AIV广西代表性分离株灭活后的HA效价≥7 log2,病毒灭活效果良好,可用于制备油乳剂灭活疫苗,且制备的油乳剂灭活疫苗稳定性和安全性良好。根据交互HI抗体滴度计算出各毒株间的抗原相关值(R),结果发现A/chicken/Guangxi/C227/2015(H9N2)株(简称C227株)与其他毒株的抗原性无明显差异,对应的R在0.72~0.93间波动;疫苗交叉保护试验结果也表明,C227株制备的油乳剂灭活疫苗对不同毒株的免疫保护率均高于80%,且免疫保护效果优于A/chicken/Guangxi/CX/2013(H9N2)株(简称CX13株),说明C227株更适合作为H9N2亚型AIV灭活疫苗的候选株。【结论】基于抗原性分析和免疫原性测定筛选出的A/chicken/Guangxi/C227/2015(H9N2)株对广西地区绝大部分H9N2亚型AIV流行株的免疫保护效果良好,可作为疫苗候选株用于研制新型H9N2亚型AIV疫苗。

民事在线诉讼当事人权益保障的检视与路径探索

当事人权益保障体现了以当事人为中心的诉讼理念,确保了实现诉讼效益与程序公正的统一。在线诉讼中单一的程序选择权类型难以满足双方当事人需求、证人远程作证提高了质证难度、异步审理稀释了直接言词原则、个人信息保护与庭审公开之间存在张力。应当根据被选择事项性质合理确定选择权类型、加强证人远程作证技术保障、肯定异步审理的价值并明确边界、具体衡量审判公开与个人信息保护价值利益。