AIM:To develop a new material for retina filling and to investigate its effect on intraocular structure and histocompatibility in rabbit eyes.METHODS:The polymer-derived hyaluronic acid(HA)was formed by UV light cross...AIM:To develop a new material for retina filling and to investigate its effect on intraocular structure and histocompatibility in rabbit eyes.METHODS:The polymer-derived hyaluronic acid(HA)was formed by UV light cross-linked with N-vinyl-pyrrolidone.Vitrectomy was performed in the rabbits,and then crosslinked HA hydrogels at different concentrations were injected.Intraocular pressure measurements,cornea check-up,and B-ultrasound examination were performed during the follow-up period.After six weeks’follow-up,the rabbits were sacrificed,and both eyes were removed for hematoxylin-eosin(HE)staining,and the polymer materials were observed under electron microscopy.RESULTS:The particle size of the cross-linked HA hydrogels was mainly around at 100 nm.After vitrectomy and injection into vitreous cavity optical coherence tomography showed that the polymeric material HA had no significant effect on the overall thickness of the retina.The intraocular pressure returned to the normal level gradually at week 4.B-ultrasound results revealed that there is no significant change in the eye tissue given to HA material.The pathological and transmission electron microscopy results showed no obvious pathological change in the primary cells and rod cells under the retina tissue.CONCLUSION:HA-based cross-linked biopolymers has good biocompatibility in rabbit eyes,showing a promising potential as vitreous substitutes.展开更多
The aim of this study was to fabricate composite gel beads based on natural polysaccharides. Hyaluronic acid（HA） and Chitosan（CS） were successfully admixed with Ca^2＋/alginate（SA） gel system to produce SA/HA/CS...The aim of this study was to fabricate composite gel beads based on natural polysaccharides. Hyaluronic acid（HA） and Chitosan（CS） were successfully admixed with Ca^2＋/alginate（SA） gel system to produce SA/HA/CS gel beads by dual crosslinking： the ionic gelation and the polyelectrolyte complexation. The preparation procedure was that the weight ratio of SA（2%, m/v） to HA（2%, m/v） was kept at 2：1, then the mixture was dripped into the Ca^2＋ solution for ion-crosslinking, and finally polyelectrolyte crosslinked with 2% low molecular weight CS（LMW-CS） for 1.5 hours. The optimal formulation was achieved by adjusting the concentration and the weight ratio of SA, HA and LMW-CS. Due to the incorporation of HA and LMWCS, the swelling ratio of the beads at pH 7.4 was increased up to 120, and the time for the maximum swelling degree was prolonged to 7.5 h. The swelling behavior was obviously improved compared to the pure SA/Ca^2＋ system. The preliminary results clearly suggest that the SA/HA/CS gel beads may be a potential candidate for biomedical delivery vehicles.展开更多
Objective: Prepare cross-linked HA gels with higher mechanical stability, lower degradation velocity and desirable biocompatibility,so as to extend the usage of HA.Method:1.Test molecular weight of HA (Mr_ HA ) by vis...Objective: Prepare cross-linked HA gels with higher mechanical stability, lower degradation velocity and desirable biocompatibility,so as to extend the usage of HA.Method:1.Test molecular weight of HA (Mr_ HA ) by viscosimetry;2.prepare cross-linked HA gels by DVS, GTA, DEC;3.discuss the cross-linking and degradation procedure;4.evaluate the biocompatibility of the best HA gels. Results:The mechanical stability and durability to degradation of HA-DVS gels are superior to those of other gels, and when HA:DVS = 40:1(g/g), at 35℃ and in 0.2 M NaOH solution, the HA-DVS gel shows the best mechanical stability, and its cytotoxicity reaches class I, hemolysis ratio is lower than 5%. Conclusion:Our HA-DVS gel can be used to prepare biologic scaffolds.展开更多
目的比较两种中度宫腔粘连治疗方案的疗效。方法将120例中度宫腔粘连患者随机分为两组,研究组：60例,采用治疗方案A：宫腔粘连分离术后立即宫腔内留置Foley导管并注入2 mL透明质酸钠凝胶（HA）,术后第3天再次于宫腔内注入2 mL HA,术后第...目的比较两种中度宫腔粘连治疗方案的疗效。方法将120例中度宫腔粘连患者随机分为两组,研究组：60例,采用治疗方案A：宫腔粘连分离术后立即宫腔内留置Foley导管并注入2 mL透明质酸钠凝胶（HA）,术后第3天再次于宫腔内注入2 mL HA,术后第4天取出Foley导管并上宫型环,同时予雌孕激素人工周期治疗3个月;对照组60例,采用治疗方案B：除术后第3天宫腔内未注入HA外,其余治疗方法均同研究组。所有患者均于术后第3次月经干净3~7 d内复查宫腔镜,观察两组的宫腔粘连复发情况及月经改善情况,以初步评价两种治疗方案的疗效。结果研究组粘连复发率15%,月经改善率90%;对照组粘连复发率31.67%,月经改善率71.67%,两组比较差异具有显著性（P值均〈0.05）。结论对于中度宫腔粘连的患者,治疗方案A的疗效明显优于治疗方案B,值得进一步探讨其临床推广应用价值。展开更多
基金Supported by Medicine and Health Science and Technology Program Project of Zhejiang Province(No.2018KY737)Natural Science Foundation Project of Zhejiang Province(No.LY19H120001)+2 种基金Ningbo Public Welfare Science and Technology Program Project(No.2019C50059)Ningbo Natural Science Foundation Project(No.2019A610353)Science and Technology Project of Yinzhou District(2017)No.110 and(2018)No.74。
文摘AIM:To develop a new material for retina filling and to investigate its effect on intraocular structure and histocompatibility in rabbit eyes.METHODS:The polymer-derived hyaluronic acid(HA)was formed by UV light cross-linked with N-vinyl-pyrrolidone.Vitrectomy was performed in the rabbits,and then crosslinked HA hydrogels at different concentrations were injected.Intraocular pressure measurements,cornea check-up,and B-ultrasound examination were performed during the follow-up period.After six weeks’follow-up,the rabbits were sacrificed,and both eyes were removed for hematoxylin-eosin(HE)staining,and the polymer materials were observed under electron microscopy.RESULTS:The particle size of the cross-linked HA hydrogels was mainly around at 100 nm.After vitrectomy and injection into vitreous cavity optical coherence tomography showed that the polymeric material HA had no significant effect on the overall thickness of the retina.The intraocular pressure returned to the normal level gradually at week 4.B-ultrasound results revealed that there is no significant change in the eye tissue given to HA material.The pathological and transmission electron microscopy results showed no obvious pathological change in the primary cells and rod cells under the retina tissue.CONCLUSION:HA-based cross-linked biopolymers has good biocompatibility in rabbit eyes,showing a promising potential as vitreous substitutes.
基金Funded by the National Natural Science Foundation of China(No.81401510)
文摘The aim of this study was to fabricate composite gel beads based on natural polysaccharides. Hyaluronic acid（HA） and Chitosan（CS） were successfully admixed with Ca^2＋/alginate（SA） gel system to produce SA/HA/CS gel beads by dual crosslinking： the ionic gelation and the polyelectrolyte complexation. The preparation procedure was that the weight ratio of SA（2%, m/v） to HA（2%, m/v） was kept at 2：1, then the mixture was dripped into the Ca^2＋ solution for ion-crosslinking, and finally polyelectrolyte crosslinked with 2% low molecular weight CS（LMW-CS） for 1.5 hours. The optimal formulation was achieved by adjusting the concentration and the weight ratio of SA, HA and LMW-CS. Due to the incorporation of HA and LMWCS, the swelling ratio of the beads at pH 7.4 was increased up to 120, and the time for the maximum swelling degree was prolonged to 7.5 h. The swelling behavior was obviously improved compared to the pure SA/Ca^2＋ system. The preliminary results clearly suggest that the SA/HA/CS gel beads may be a potential candidate for biomedical delivery vehicles.
基金The Second Hospital of Tianjin Medical University,300211 Tianjin,China
文摘Objective: Prepare cross-linked HA gels with higher mechanical stability, lower degradation velocity and desirable biocompatibility,so as to extend the usage of HA.Method:1.Test molecular weight of HA (Mr_ HA ) by viscosimetry;2.prepare cross-linked HA gels by DVS, GTA, DEC;3.discuss the cross-linking and degradation procedure;4.evaluate the biocompatibility of the best HA gels. Results:The mechanical stability and durability to degradation of HA-DVS gels are superior to those of other gels, and when HA:DVS = 40:1(g/g), at 35℃ and in 0.2 M NaOH solution, the HA-DVS gel shows the best mechanical stability, and its cytotoxicity reaches class I, hemolysis ratio is lower than 5%. Conclusion:Our HA-DVS gel can be used to prepare biologic scaffolds.
文摘目的比较两种中度宫腔粘连治疗方案的疗效。方法将120例中度宫腔粘连患者随机分为两组,研究组：60例,采用治疗方案A：宫腔粘连分离术后立即宫腔内留置Foley导管并注入2 mL透明质酸钠凝胶（HA）,术后第3天再次于宫腔内注入2 mL HA,术后第4天取出Foley导管并上宫型环,同时予雌孕激素人工周期治疗3个月;对照组60例,采用治疗方案B：除术后第3天宫腔内未注入HA外,其余治疗方法均同研究组。所有患者均于术后第3次月经干净3~7 d内复查宫腔镜,观察两组的宫腔粘连复发情况及月经改善情况,以初步评价两种治疗方案的疗效。结果研究组粘连复发率15%,月经改善率90%;对照组粘连复发率31.67%,月经改善率71.67%,两组比较差异具有显著性（P值均〈0.05）。结论对于中度宫腔粘连的患者,治疗方案A的疗效明显优于治疗方案B,值得进一步探讨其临床推广应用价值。
