The safety of feeding transgenic crylAb/crylAc rice （a genetically modified （GM） rice variety） to broilers was examined from an immunological perspective. HatchlingArborAcres chickens （240） were assigned to two ...The safety of feeding transgenic crylAb/crylAc rice （a genetically modified （GM） rice variety） to broilers was examined from an immunological perspective. HatchlingArborAcres chickens （240） were assigned to two dietary treatments （diets containing GM or non-GM rice） with 12 replicates per group and 10 birds per replicate. Traits were measured on one randomly selected bird from each replicate at d 21 and 42. The 42-d feeding trial revealed that crylAb/crylAc rice had no significant effect relative to non-GM rice on body weight and the immune organ indices. No significant pathological lesion in the spleen and bursa of Fabricius was found in the GM rice group. There were no significant differences in serum concentrations of immunoglobulin Y （IgY）, IgM, interleukin 4 （IL-4） and IL-6 between the two groups at d 21 or 42, except for IL-6 being higher （P〈0.05） in the GM- fed chickens at d 42. There were no differences in the T and B lymphocyte transformation rate and CD4＋/CD8＋ ratio between the two groups at d 42. Additionally, there was no significant difference between the two diets in expression of relevant genes viz. the major histocompatibility complex class II beta chain （BLB2）, interferon beta 1 （IFNβ）, tumour necrosis factor alpha-like （TNFa） and toll-like receptor 4 （TLR4）in the spleen and bursa of Fabricius. All the data demonstrated that transgenic crylAb/ crylAc rice had no adverse effect on these aspects of immune function of broilers during 42-d feeding trial. Transgenic rice was therefore indistinguishable from non-GM rice in terms of short-term feeding in chickens.展开更多
This study was conducted to build a recombinant strain with highly insecticidal activity and a wide host range by using the crylAc and p74 gene. Firstly, the p74 gene was amplified from the genosome ofAutographa calif...This study was conducted to build a recombinant strain with highly insecticidal activity and a wide host range by using the crylAc and p74 gene. Firstly, the p74 gene was amplified from the genosome ofAutographa californica multicapsid nucleopolyhedrovirus. The crylAc gene and the terminator gene of crylAc, named crylAct, were amplified from the plasmid of Bt 4.0718 strain. Three T vectors, named pTp74, pT1Ac, and pT1Act which held the aimed gene p74, cry1Ac, and crylAct, respectively, and two middle vectors, named pTp74Act and pTIAcp74 which held the aimed fusion gene p74-crylAct and cry lAc-p74, respectively, were built by using pMDI 8-T. Then pTiAcp74 and the shuttle plasmid were digested and linked and an expressing-vector pH1Acp74 was built. Finally, pH1Acp74 was transformed into the acrystalliferous strain XBU001 and the aimed recombinant strain XBU-H1Acp74 was obtained. The expression of Bt transformant XBU-H1Acp74 was analyzed by SDS-PAGE which showed XBU-H1Acp74 could produce 130 kDa CrylAc protein and 50 kDa P74 protein. The insecticidal activity of transformant against Spodoptera exigua was evaluated compared with the contrast strains HTX-42 （only crylAc gene was transformed into XBU001） after autolysis. The LCs0 of HTX-42 was higher than that of the XBU-H IAcp74＇s, which implied that P74 could increase the efficacy and range of Bt Cry toxins in insect control. The fusion gene of crylAc and p74 were constructed successfully which will be served as the foundation lbr constructing the fusion genes of Bt cry gene and other foreign genes.展开更多
基金supported by the grants from the National Science and Technology Major Project of the Ministry of Science and Technology of China (2012ZX08011001005)the Agricultural Science and Technology Innovation Program, China (ASTIP-IAS04)the Earmarked Fund for Modern Agro-Industry Technology Research System, China (CARS-42)
文摘The safety of feeding transgenic crylAb/crylAc rice （a genetically modified （GM） rice variety） to broilers was examined from an immunological perspective. HatchlingArborAcres chickens （240） were assigned to two dietary treatments （diets containing GM or non-GM rice） with 12 replicates per group and 10 birds per replicate. Traits were measured on one randomly selected bird from each replicate at d 21 and 42. The 42-d feeding trial revealed that crylAb/crylAc rice had no significant effect relative to non-GM rice on body weight and the immune organ indices. No significant pathological lesion in the spleen and bursa of Fabricius was found in the GM rice group. There were no significant differences in serum concentrations of immunoglobulin Y （IgY）, IgM, interleukin 4 （IL-4） and IL-6 between the two groups at d 21 or 42, except for IL-6 being higher （P〈0.05） in the GM- fed chickens at d 42. There were no differences in the T and B lymphocyte transformation rate and CD4＋/CD8＋ ratio between the two groups at d 42. Additionally, there was no significant difference between the two diets in expression of relevant genes viz. the major histocompatibility complex class II beta chain （BLB2）, interferon beta 1 （IFNβ）, tumour necrosis factor alpha-like （TNFa） and toll-like receptor 4 （TLR4）in the spleen and bursa of Fabricius. All the data demonstrated that transgenic crylAb/ crylAc rice had no adverse effect on these aspects of immune function of broilers during 42-d feeding trial. Transgenic rice was therefore indistinguishable from non-GM rice in terms of short-term feeding in chickens.
基金supported by the National Natural Science Foundation of China (30570050, 30670052)the National High Technology R&D Program of China (863Program, 2006AA02Z187)+1 种基金the National Ph D Programs Foundation of China (200-60542006)the Natural Science Foundation of Hunan Province, China(06JJ50062, 06JJ2009).
文摘This study was conducted to build a recombinant strain with highly insecticidal activity and a wide host range by using the crylAc and p74 gene. Firstly, the p74 gene was amplified from the genosome ofAutographa californica multicapsid nucleopolyhedrovirus. The crylAc gene and the terminator gene of crylAc, named crylAct, were amplified from the plasmid of Bt 4.0718 strain. Three T vectors, named pTp74, pT1Ac, and pT1Act which held the aimed gene p74, cry1Ac, and crylAct, respectively, and two middle vectors, named pTp74Act and pTIAcp74 which held the aimed fusion gene p74-crylAct and cry lAc-p74, respectively, were built by using pMDI 8-T. Then pTiAcp74 and the shuttle plasmid were digested and linked and an expressing-vector pH1Acp74 was built. Finally, pH1Acp74 was transformed into the acrystalliferous strain XBU001 and the aimed recombinant strain XBU-H1Acp74 was obtained. The expression of Bt transformant XBU-H1Acp74 was analyzed by SDS-PAGE which showed XBU-H1Acp74 could produce 130 kDa CrylAc protein and 50 kDa P74 protein. The insecticidal activity of transformant against Spodoptera exigua was evaluated compared with the contrast strains HTX-42 （only crylAc gene was transformed into XBU001） after autolysis. The LCs0 of HTX-42 was higher than that of the XBU-H IAcp74＇s, which implied that P74 could increase the efficacy and range of Bt Cry toxins in insect control. The fusion gene of crylAc and p74 were constructed successfully which will be served as the foundation lbr constructing the fusion genes of Bt cry gene and other foreign genes.
