Aim:To identify genes related to the human testis development by substrate hybridization technique.Methods:A human testis cDNA microarray was constructed and hybridized with probes prepared from human adult and fetal ...Aim:To identify genes related to the human testis development by substrate hybridization technique.Methods:A human testis cDNA microarray was constructed and hybridized with probes prepared from human adult and fetal testes and spermatozoa mRNAs by reverse transcription reactions.The differentially expressed genes were sequenced. And a newly identified cullin-3 (CUL-3) transcript variant (designated cul-3b) was bio-informatically analyzed with an online GenBank database.Multi-tissue reverse transcription polymerase chain reaction (RT-PCR) was used to deter- mine the tissue expression profile of cul-3b.Results:Cul-3b,a novel CUL-3 transcript variant,was identified.The expression level of cul-3b in adult testes was 3.79-fold higher than that in fetal ones.Cul-3b differed from cul-3 (including NM_003590 and AY337761) in the opening reading frame and had three internal ribosomal entry sites (IRESes) in the 5'-UTR.These led to a 24 amino acid (aa) truncation at N-terminus of CUL-3b as compared with CUL-3 and a more motivated expression pattern of cul-3b under some strict circumstances.Additionally,cul-3b expressed ubiquitously in human tissues according to multi-tissue RT-PCR.Conclusion:Cul-3b is a novel transcript variant of CUL-3,which may be important not only for the development of human testis but also for that of other organs.展开更多
This paper describes recent levels of surfactants measured in a coastal lagoon ecosystem highly stressed by human activites: the Orbetello lagoon (Southern Tuscany, Italy, Ramsar Site IT008). Significance of differenc...This paper describes recent levels of surfactants measured in a coastal lagoon ecosystem highly stressed by human activites: the Orbetello lagoon (Southern Tuscany, Italy, Ramsar Site IT008). Significance of difference among concentrations measured before and after summertime are explored in order to evaluate effects related to tourism exploitation. Among surfactants, methylene blue active anionic substances (MBAS) are selected as tracers for untreated discharges originated by domestic and urban activities. Water samplings were performed in 2011 following a randomly replicated nested logic model (n = 144). MBAS mean level of 0.070 mg.L–1 (–1) and 0.530 mg.L–1 (–1) are respectively recorded in June and October evidencing a significant increase after the touristic season. Possible MBAS concentration phenomena could be induced by different evaportaton rates among sampling stations and between winter and summer seasons and were evaluated, in this study, using water salinity as possible factor affecting samples segregations. Results evidence that differences of MBAS levels related to evaporation rates are trascurabile if compared to the variability induced by the presence of not-collected wastewater hot-spot pollution sources located closed around the urban settlement and along sandbars. Measured levels of surfactants could act negatively on living organisms both animals and plants contributing to affect Orbetello lagoon biodiversity. In this ecosystem, after the complete reorganization of urban wastewater treatment plants occured in 2008, further management actions should be focalized on reducing untreated sources of MBAS.展开更多
The effects of pigment glands and gossypol on the somatic cell culture of upland cotton were studied, using the materials as follows: three pairs of glanded and gland less upland cotton near isogenic lines, TM-1, and ...The effects of pigment glands and gossypol on the somatic cell culture of upland cotton were studied, using the materials as follows: three pairs of glanded and gland less upland cotton near isogenic lines, TM-1, and Coker 312. The results showed that the pigment glands and gossypol contents in the explants had great inhibiting effect on the induction and growth of callus in somatic cell culture of upland cotton, and the induction rate of callus and the single callus weight of glandless cotton were much higher than those of their glanded near isogenic lines. It was easier to obtain regeneration plants from glandless cotton than from their glandednear isogenic lines. There was a significant inverse correlation between the gossypol contents in the explants and callus induction rate, with the correlation coefficient of -0.84. The vitro gossypol in the medium had some inhibiting effect on the induction and growth of callus, especially for the gland-less cotton. However, a certain concentration of vitro展开更多
文摘Aim:To identify genes related to the human testis development by substrate hybridization technique.Methods:A human testis cDNA microarray was constructed and hybridized with probes prepared from human adult and fetal testes and spermatozoa mRNAs by reverse transcription reactions.The differentially expressed genes were sequenced. And a newly identified cullin-3 (CUL-3) transcript variant (designated cul-3b) was bio-informatically analyzed with an online GenBank database.Multi-tissue reverse transcription polymerase chain reaction (RT-PCR) was used to deter- mine the tissue expression profile of cul-3b.Results:Cul-3b,a novel CUL-3 transcript variant,was identified.The expression level of cul-3b in adult testes was 3.79-fold higher than that in fetal ones.Cul-3b differed from cul-3 (including NM_003590 and AY337761) in the opening reading frame and had three internal ribosomal entry sites (IRESes) in the 5'-UTR.These led to a 24 amino acid (aa) truncation at N-terminus of CUL-3b as compared with CUL-3 and a more motivated expression pattern of cul-3b under some strict circumstances.Additionally,cul-3b expressed ubiquitously in human tissues according to multi-tissue RT-PCR.Conclusion:Cul-3b is a novel transcript variant of CUL-3,which may be important not only for the development of human testis but also for that of other organs.
文摘This paper describes recent levels of surfactants measured in a coastal lagoon ecosystem highly stressed by human activites: the Orbetello lagoon (Southern Tuscany, Italy, Ramsar Site IT008). Significance of difference among concentrations measured before and after summertime are explored in order to evaluate effects related to tourism exploitation. Among surfactants, methylene blue active anionic substances (MBAS) are selected as tracers for untreated discharges originated by domestic and urban activities. Water samplings were performed in 2011 following a randomly replicated nested logic model (n = 144). MBAS mean level of 0.070 mg.L–1 (–1) and 0.530 mg.L–1 (–1) are respectively recorded in June and October evidencing a significant increase after the touristic season. Possible MBAS concentration phenomena could be induced by different evaportaton rates among sampling stations and between winter and summer seasons and were evaluated, in this study, using water salinity as possible factor affecting samples segregations. Results evidence that differences of MBAS levels related to evaporation rates are trascurabile if compared to the variability induced by the presence of not-collected wastewater hot-spot pollution sources located closed around the urban settlement and along sandbars. Measured levels of surfactants could act negatively on living organisms both animals and plants contributing to affect Orbetello lagoon biodiversity. In this ecosystem, after the complete reorganization of urban wastewater treatment plants occured in 2008, further management actions should be focalized on reducing untreated sources of MBAS.
基金This work was supported by the National Natural Science Foundation of China (Grant Nos. 39970467 and 39830240) Zhejiang Provincial Natural Science Foundation (Grant No. 398260).
文摘The effects of pigment glands and gossypol on the somatic cell culture of upland cotton were studied, using the materials as follows: three pairs of glanded and gland less upland cotton near isogenic lines, TM-1, and Coker 312. The results showed that the pigment glands and gossypol contents in the explants had great inhibiting effect on the induction and growth of callus in somatic cell culture of upland cotton, and the induction rate of callus and the single callus weight of glandless cotton were much higher than those of their glanded near isogenic lines. It was easier to obtain regeneration plants from glandless cotton than from their glandednear isogenic lines. There was a significant inverse correlation between the gossypol contents in the explants and callus induction rate, with the correlation coefficient of -0.84. The vitro gossypol in the medium had some inhibiting effect on the induction and growth of callus, especially for the gland-less cotton. However, a certain concentration of vitro