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Entrepreneurial Spirit for China in the New Era and Strategies for Cultivation
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作者 Xu Yuanhua He Jun +1 位作者 Sun Zao Li Xiaoke 《China Economist》 2024年第5期48-59,共12页
What type of entrepreneurial spirit is needed for China’s new era,and how can we unlock its innovation potential?Answers to these questions will unleash vital forces to drive China’s high-quality economic developmen... What type of entrepreneurial spirit is needed for China’s new era,and how can we unlock its innovation potential?Answers to these questions will unleash vital forces to drive China’s high-quality economic development.After reviewing the theories,dimensions,features,and limitations of the entrepreneurial spirit in the Western context,we concluded that the entrepreneurial spirit in China’s new era,which is characterized by patriotism,innovation,trustworthiness,adherence to the law,fulfillment of social responsibilities,and a global horizon,has overcome the limitations that come with self-interested entrepreneurship in the context of Western economics.This is a major step forward in the development of theories on entrepreneurial spirit.In the development context of the new era,this paper argues that the entrepreneurial spirit should become an important force at the individual level to implement the new development concept,foster the new development paradigm,and promote high-quality development in the new stage.This paper provides answers to the question of what type of entrepreneurial spirit China’s new era requires.Finally,this paper presents basic strategies for cultivating entrepreneurial spirit in the context of China’s new era,emphasizing the importance to promote the healthy growth of young entrepreneurs,ensure their business autonomy,develop a multi-tiered capital market,implement the factor market reforms,improve the business climate,and accelerate the digital transition. 展开更多
关键词 Context of China’s new era context of Western economics entrepreneurial spirit high-quality development cultivation strategy
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Discussion and Practice on Talent Cultivation Model Based on OBE Concept in Veterinary Medicine Graduate Education
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作者 Jiedan Liao 《Journal of Contemporary Educational Research》 2024年第11期16-22,共7页
To address the challenges in training veterinary master’s students,it is essential to align with the core objectives of veterinary graduate education.This involves focusing on faculty development,course design,innova... To address the challenges in training veterinary master’s students,it is essential to align with the core objectives of veterinary graduate education.This involves focusing on faculty development,course design,innovative teaching methods,university-industry collaboration,and reforming the evaluation system.By applying the outcome-based education model,new approaches to veterinary graduate training can be developed,particularly for applied talent development in agricultural universities serving local communities.This innovative model emphasizes ideological and political education,aiming to enhance students’abilities in knowledge and practical innovation,preparing them to be well-rounded professionals.The goal is to cultivate senior veterinary experts who are not only skilled in academic research and scientific inquiry but also committed to advancing agriculture,rural areas,and supporting rural revitalization.This approach provides a valuable framework for fostering highly qualified graduates who are both technically proficient and socially responsible. 展开更多
关键词 Outcome-based education Veterinary medicine Master’s students Talent cultivation mode
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The Independence-Separation Problem on the 3-D Rook’s Graph
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作者 Paul A. Burchett 《Open Journal of Discrete Mathematics》 2016年第3期167-173,共7页
Both independence and independence-separation problems on chessboard graphs have been studied in detail, with hundreds of papers in the broader independence category, and several on the independence-separation problem... Both independence and independence-separation problems on chessboard graphs have been studied in detail, with hundreds of papers in the broader independence category, and several on the independence-separation problem variant for chessboard graphs. In this paper, the inde-pendence-separation problem is considered on the d-dimensional rook’s graph. A lower bound of k, for , is found for the independence-separation number on the d-dimensional rook’s graph, denoted by . For the case where , it is found that when n is odd and , . Conjecture and discussion are added. 展开更多
关键词 CHEss independence-separation Number independence Number p-Dimensional Grid-Line Graphs p-Dimensional Rook’s Graph
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Research on the Cultivation of Craftsmanship in Huang Yanpei’s Education Thought
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作者 Shang Wang Fei Peng Jie Qiu 《Journal of Contemporary Educational Research》 2023年第3期14-19,共6页
Skilled talents are the main support for the strategic development of science and technology in China.The quality of talents is an important guarantee for the transformation and upgrading of national industries.By ana... Skilled talents are the main support for the strategic development of science and technology in China.The quality of talents is an important guarantee for the transformation and upgrading of national industries.By analyzing and studying Huang Yanpei’s thought on vocational education,this paper focuses on the cultivation of students’craftsmanship in vocational schools.First,we analyze the results of previous research and then elaborate on the connotations of Huang Yanpei’s education thought and craftsmanship spirit.Lastly,we propose five suggestions for the cultivation of craftsmanship.Our research work carries certain reference value to the study of craftsmanship cultivation in Huang Yanpei’s education thought. 展开更多
关键词 cultivation of craftsmanship Craftsmanship TEACHING Huang Yanpei’s education thought
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Analysis of cultivable aerobic bacterial community composition and screening for facultative sulfate-reducing bacteria in marine corrosive steel 被引量:5
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作者 LI Xiaohong XIAO Hui +7 位作者 ZHANG Wenjun LI Yongqian TANG Xuexi DUAN Jizhou YANG Zhibo WANG Jing GUAN Fang DING Guoqing 《Journal of Oceanology and Limnology》 SCIE CAS CSCD 2019年第2期600-614,共15页
Anaerobic, aerobic, and facultative bacteria are all present in corrosive environments. However, as previous studies to address corrosion in the marine environment have largely focused on anaerobic bacteria, limited a... Anaerobic, aerobic, and facultative bacteria are all present in corrosive environments. However, as previous studies to address corrosion in the marine environment have largely focused on anaerobic bacteria, limited attention has been paid to the composition and function of aerobic and facultative bacteria in this process. For analysis in this study, ten samples were collected from rust layers on steel plates that had been immersed in seawater for diff erent periods (i.e., six months and eight years) at Sanya and Xiamen, China. The cultivable aerobic bacterial community structure as well as the number of sulfate-reducing bacteria (SRB) were analyzed in both cases, while the proportion of facultative SRB among the isolated aerobic bacteria in each sample was also evaluated using a novel approach. Bacterial abundance results show that the proportions are related to sea location and immersion time;abundances of culturable aerobic bacteria (CAB) and SRB from Sanya were greater in most corrosion samples than those from Xiamen, and abundances of both bacterial groups were greater in samples immersed for six months than for eight years. A total of 213 isolates were obtained from all samples in terms of CAB community composition, and a phylogenetic analysis revealed that the taxa comprised four phyla and 31 genera. Bacterial species composition is related to marine location;the results show that Firmicutes and Proteobacteria were the dominant phyla, accounting for 98.13% of the total, while Bacillus and Vibrio were the dominant genera, accounting for 53.06% of the total. An additional sixfacultative SRB strains were also screened from the isolates obtained and were found to encompass the genus Vibrio (four strains), Staphylococcus (one strain), and Photobacterium (one strain). It is noteworthy that mentions of Photobacterium species have so far been absent from the literature, both in terms of its membership of the SRB group and its relationship to corrosion. 展开更多
关键词 MARINE corrosive sTEEL cultivable AEROBIC BACTERIA FACULTATIVE sulfate-reducing BACTERIA bacterial community composition 16s RRNA gene sequencing
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U-G-S协同推进创新人才早期培养的国际经验与启示
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作者 陆超 金辉召 黄雪君 《教育参考》 2024年第6期43-52,84,共11页
本文选取美国、澳大利亚、新加坡等创新人才机制相对成熟的国家,探究其创新人才多主体早期培育项目经验,分析、归纳得出主要培养路径有四:首先,“丰富和加速”是主要培养手段,以高阶课程助力超前学习、以缩短学制促进加速学习、以暑期... 本文选取美国、澳大利亚、新加坡等创新人才机制相对成熟的国家,探究其创新人才多主体早期培育项目经验,分析、归纳得出主要培养路径有四:首先,“丰富和加速”是主要培养手段,以高阶课程助力超前学习、以缩短学制促进加速学习、以暑期项目丰富课程学习;其次,以信息技术搭建资优教育平台,扩展资源网络渠道,推进创新人才培养的机会均等;再次,注重教育体系衔接,以大中小衔接体系为创新人才接续培养枢纽,确保培养质量;最后,以分类培养理念创新人才和技术创新人才为目标,建立针对性培养体系。据此,本文提出改进我国创新人才早期培养U-G-S协作机制的建议:加强政策顶层设计、扶持校企联合、增强创新人才网络资源建设、推进校本创新人才培养项目建设等。 展开更多
关键词 创新人才 早期培养 U-G-s
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Efficient Fast Independent Component Analysis Algorithm with Fifth-Order Convergence
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作者 Xuan-Sen He Tiao-Jiao Zhao Fang Wang 《Journal of Electronic Science and Technology》 CAS 2011年第3期244-249,共6页
Independent component analysis (ICA) is the primary statistical method for solving the problems of blind source separation. The fast ICA is a famous and excellent algorithm and its contrast function is optimized by ... Independent component analysis (ICA) is the primary statistical method for solving the problems of blind source separation. The fast ICA is a famous and excellent algorithm and its contrast function is optimized by the quadratic convergence of Newton iteration method. In order to improve the convergence speed and the separation precision of the fast ICA, an improved fast ICA algorithm is presented. The algorithm introduces an efficient Newton's iterative method with fifth-order convergence for optimizing the contrast function and gives the detail derivation process and the corresponding condition. The experimental results demonstrate that the convergence speed and the separation precision of the improved algorithm are better than that of the fast ICA. 展开更多
关键词 Index Terms---Blind source separation fast independent component analysis fifth-order convergence independent component analysis Newton's iterative method.
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师范生U-G-S培养模式实践探索与路径优化——基于“优师计划”背景
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作者 高鹏飞 蒋丽 +2 位作者 尹婷婷 任翠香 马丽贞 《唐山师范学院学报》 2024年第5期127-130,共4页
师范生U-G-S培养模式实践已取得诸多成效,如多方配合助力师范生培养,促进区域均衡,提升基层教学水平等。但师范生U-G-S培养模式在实践中也存在突出问题,如高校一方主导、其他方参与度低,难以高效协同,培育方案与实际岗位需求存在适配偏... 师范生U-G-S培养模式实践已取得诸多成效,如多方配合助力师范生培养,促进区域均衡,提升基层教学水平等。但师范生U-G-S培养模式在实践中也存在突出问题,如高校一方主导、其他方参与度低,难以高效协同,培育方案与实际岗位需求存在适配偏差等。剖析“优师计划”对优化师范生U-G-S培养模式的启发,探索以当地政府为统筹、师范院校为关键、中小学校为导向、师范生为主体的“四位一体”优化路径,有助于提高师范生培育成效。 展开更多
关键词 “优师计划” 师范生培养 U-G-s培养模式
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China's 15-year-old Postdoc System Cultivates 13,100 High-level Professionals
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《Bulletin of the Chinese Academy of Sciences》 2000年第4期198-198,共1页
Since its founding in 1985, China’s Post-doc system has cultivated a total of 13,100 high-level professionals, a national conference of postdocs revealed recently. The program’s establishment was given the green lig... Since its founding in 1985, China’s Post-doc system has cultivated a total of 13,100 high-level professionals, a national conference of postdocs revealed recently. The program’s establishment was given the green light by Deng Xiaoping in 1985 in response to a suggestion by Prof. Tsung Dao Lee, a Nobel physics prize laureate. Starting from scratch, so far 798 programs for postdocs have been established, to be distributed in 展开更多
关键词 CAs Pr China’s 15-year-old Postdoc system cultivates 13 100 High-level Professionals
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“优师计划”背景下师范生U-G-S培养模式价值、问题及对策
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作者 尹婷婷 蒋丽 高鹏飞 《唐山师范学院学报》 2024年第3期106-109,共4页
从“优师计划”背景下师范生U-G-S培养模式价值分析入手,剖析“优师计划”背景下师范生U-G-S培养模式中地方政府、师范院校、中小学校、师范生四个基本要素各自存在问题,提出“四位一体”师范生U-G-S培养模式提升对策,以当地政府为主导... 从“优师计划”背景下师范生U-G-S培养模式价值分析入手,剖析“优师计划”背景下师范生U-G-S培养模式中地方政府、师范院校、中小学校、师范生四个基本要素各自存在问题,提出“四位一体”师范生U-G-S培养模式提升对策,以当地政府为主导、以师范院校为引领、以中小学校为核心、以师范生为主体,统筹各方培养资源和优势力量,激活多方利益主体意愿,助力乡村卓越教师的培养。 展开更多
关键词 “优师计划” 师范生 U-G-s培养模式
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Research on the Influence of Family Education on Children’s Behavioral Habits
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作者 Jiayi Wen 《Journal of Contemporary Educational Research》 2024年第8期176-180,共5页
Under the background of the all-round deepening of quality education,the cultivation of comprehensive quality has become the main theme of contemporary education reform.Good behavior and habits are of great significan... Under the background of the all-round deepening of quality education,the cultivation of comprehensive quality has become the main theme of contemporary education reform.Good behavior and habits are of great significance to children’s future learning,growth,and development.Through literature review and other methods,this paper analyzes the current situation of children’s family education and the influence of family education on the cultivation of children’s behavioral habits and provides some strategies for cultivating children’s good behavioral habits in family education. 展开更多
关键词 Family education Children’s behavior Habit cultivation PARENTs
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SAM产生菌酿酒酵母HYS98发酵动力学及比生长速率控制策略 被引量:12
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作者 张昀羿 李元广 +2 位作者 金建 杨东 沈国敏 《过程工程学报》 EI CAS CSCD 北大核心 2005年第3期322-326,共5页
通过恒化培养对S-腺苷甲硫氨酸(SAM)产生菌酿酒酵母HYS98发酵动力学进行了研究.结果表明,该菌株的生长与限制性基质蔗糖浓度之间符合Monod关系式,最大比生长速率μmax为0.28h-1,饱和常数KS为1.27g/L;当0.005h?1≤μ≤0.11h-1,产物生成... 通过恒化培养对S-腺苷甲硫氨酸(SAM)产生菌酿酒酵母HYS98发酵动力学进行了研究.结果表明,该菌株的生长与限制性基质蔗糖浓度之间符合Monod关系式,最大比生长速率μmax为0.28h-1,饱和常数KS为1.27g/L;当0.005h?1≤μ≤0.11h-1,产物生成模型为qSAM=-1.39μ2+0.19μ,底物消耗动力学模型为qS=μ/0.62+0.012.基于建立的动力学模型,提出了分阶段控制菌体比生长速率的流加培养策略,据此策略在5L反应器中发酵24h,SAM浓度达3.56g/L,而SAM产率达0.15g/(L?h),比文献报道的最高水平高67%. 展开更多
关键词 s-腺苷甲硫氨酸 恒化培养 动力学模型 流加培养
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嗜蓝孢孔菌新种Fomitiporia yanbeiensis S. Guo&L. Zhou液体发酵培养条件研究 被引量:4
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作者 刘晓钢 周林 +4 位作者 徐莉娜 李艳婷 周伟 郭尚 侯雷平 《山西农业大学学报(自然科学版)》 CAS 北大核心 2018年第5期71-76,共6页
[目的]本研究是对Fomitiporia yanbeiensis S.Guo&L.Zhou液体发酵培养基的营养源和培养条件进行探索。[方法]以发酵培养液中的菌球生物量、菌丝生长速度和菌球萌发活力指数为主要指标,通过单因素试验和正交试验对发酵工艺的培养的... [目的]本研究是对Fomitiporia yanbeiensis S.Guo&L.Zhou液体发酵培养基的营养源和培养条件进行探索。[方法]以发酵培养液中的菌球生物量、菌丝生长速度和菌球萌发活力指数为主要指标,通过单因素试验和正交试验对发酵工艺的培养的温度、pH值、碳源、氮源、接种量进行优化筛选,研究最佳的发酵培养基配方,并对发酵菌丝体和子实体的功能性成分测定对比。[结果]液体发酵培养中最佳的发酵培养条件为:温度27℃、起始pH为8.0、接种量为11%、碳源是海藻糖、氮源是豆粕粉,最佳的培养基配方为海藻糖2.0%、豆粕粉3.5%、KH_2PO_4(0.05%)、MgSO_4(0.025%),该条件下菌球生物量最高为2.36g·100mL^(-1)。发酵菌丝体的三萜类化合物、β-葡聚糖和黄酮含量均高于子实体。[结论]通过对发酵工艺的筛选优化,为该菌的人工驯化和功能性食品开发奠定了基础。 展开更多
关键词 Fomitiporia yanbeiensis s. Guo&L. ZHOU 液体发酵 培养条件
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酒精酵母Saccharomyces cerevisiae 4-S的高密度培养及其酒精发酵性能 被引量:9
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作者 段学辉 牛春铃 欧阳军梅 《南昌大学学报(工科版)》 CAS 2008年第4期311-315,共5页
实验比较了分批培养、分批补料及连续流加培养对酒精酵母细胞生长的影响。结果显示,分批补料流加或连续流加(流加速率0.133 g.L-1.m-1)有利于酒精酵母菌Saccharomyces cerevisiae4-S的快速生长和得到较高的细胞浓度,分批补料流加或连续... 实验比较了分批培养、分批补料及连续流加培养对酒精酵母细胞生长的影响。结果显示,分批补料流加或连续流加(流加速率0.133 g.L-1.m-1)有利于酒精酵母菌Saccharomyces cerevisiae4-S的快速生长和得到较高的细胞浓度,分批补料流加或连续流加培养44 h,发酵液中酒精酵母细胞浓度分别达到71.82(细胞干重-DCW)g.L-1和82.01(DCW)g.L-1,比分批培养分别提高了155.68%和191.95%。同时,对高密度酒精酵母的酒精发酵性能和循环利用研究结果显示,在细胞浓度为82.01(DCW)g.L-1,糖浓度为350 g.L-1,发酵36h发酵液中乙醇浓度达到16.23%,比普通酵母浓度28.09(DCW)g.L-1批发酵时间缩短12 h,乙醇浓度提高了24.4%,酵母的有效重复利用次数达4批次。 展开更多
关键词 酒精酵母 高密度培养 发酵性能
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面向家纺企业的B/S结构iS-ERP系统设计与实现 被引量:3
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作者 高家全 蔡铁峰 《计算机工程与应用》 CSCD 北大核心 2006年第7期211-213,226,共4页
文章以家纺企业面临的实际情况为背景,为解决企业管理瓶颈,设计了一种以销、产、供为核心、与平台无关、柔韧性极强的B/S结构的ERP系统——iS-ERP。并详细介绍了此系统的设计和实现方法。应用结果数据显示此系统具有较好的应用效果。
关键词 平台无关 B/s ERP Is-ERP
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独立学院转型发展的逻辑理性和实践指向--基于“K-S”组合分析模式的研究 被引量:9
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作者 罗晓庆 范明 《高校教育管理》 CSSCI 北大核心 2018年第5期54-61,共8页
文章通过对独立学院转型发展的路径演化过程进行梳理,指出独立学院的转型发展具有在内外因共同作用下,以创新为内核,以"发展-调适-优化-再发展"为演化路径的内在逻辑理性。在此基础上,文章构建"KS"组合分析模式对... 文章通过对独立学院转型发展的路径演化过程进行梳理,指出独立学院的转型发展具有在内外因共同作用下,以创新为内核,以"发展-调适-优化-再发展"为演化路径的内在逻辑理性。在此基础上,文章构建"KS"组合分析模式对作用于独立学院转型发展的相关要素进行关键性评估和战略分析,进而探讨不同院校基于自身发展的实践路径:基础较好且优势明显的院校应加大创新力度,创建高水平应用型本科高校;基础扎实但优势尚不明显的院校应加强教育教学改革,提升办学的综合实力和创新人才培养能力;具有一定优势但存在办学风险的院校应强化办学特色,理顺各方关系,规范办学秩序,防控办学风险;办学实力较弱且面临一定困境的院校,应着力改进存在的不足,夯实育人质量,解决运行机制及管理体系方面的问题。 展开更多
关键词 独立学院 本科职业教育 转型发展 “K-s”组合分析模式
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结合广义S变换和快速独立分量分析的局放信号中窄带干扰抑制方法 被引量:5
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作者 李向群 伍亚萍 +2 位作者 庄旭菲 吉飞敏 张燕 《现代电力》 北大核心 2022年第5期597-604,共8页
针对现有方法对局部放电(partial discharge,PD)信号中窄带干扰抑制效果较差的问题,提出了一种基于广义S变换和快速独立分量分析的窄带干扰抑制方案。该方案首先利用广义S变换对染噪PD信号进行时频分析,在染噪PD信号的时频分布图中,根... 针对现有方法对局部放电(partial discharge,PD)信号中窄带干扰抑制效果较差的问题,提出了一种基于广义S变换和快速独立分量分析的窄带干扰抑制方案。该方案首先利用广义S变换对染噪PD信号进行时频分析,在染噪PD信号的时频分布图中,根据窄带干扰和PD信号的不同时频特征,可以确定窄带干扰的特征区域;然后在窄带干扰的特征区域中,利用Candan算法对窄带干扰的频率进行估计;最后利用窄带干扰频率估计值和快速独立分量分析方法分离出PD信号,实现窄带干扰抑制。仿真和实测结果说明,所提方法可以有效抑制染噪PD信号中窄带干扰,并且能准确获取PD信号的波形特征,对比传统的奇异值分解方法和快速傅里叶变换滤波方法,文中方法对窄带干扰的抑制效果更好,残余干扰的能量较小。 展开更多
关键词 局部放电 窄带干扰 广义s变换 Candan算法 快速独立分量分析
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Chemostat系统中的Hopf分支 被引量:7
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作者 刘婧 郑斯宁 《大连理工大学学报》 CAS CSCD 北大核心 2002年第4期387-390,共4页
讨论了一类微生物连续培养模型的 Hopf分支问题 .该模型的消耗率参数中含有一个关于营养基的二次函数 ,功能反应函数则为具有内代谢的 Monod类型 ,以更好地模拟实际问题 .利用 Friedrich方法得到了该系统存在 Hopf分支的条件 ,并判定了... 讨论了一类微生物连续培养模型的 Hopf分支问题 .该模型的消耗率参数中含有一个关于营养基的二次函数 ,功能反应函数则为具有内代谢的 Monod类型 ,以更好地模拟实际问题 .利用 Friedrich方法得到了该系统存在 Hopf分支的条件 ,并判定了周期解的稳定性 . 展开更多
关键词 Chemostat系统 周期解 稳定性 Friedrich方法 微生物连续培养模型 HOPF分支 Monod模型
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中国野生和栽培大豆11S及7S蛋白质相对含量的比较分析 被引量:6
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作者 刘顺湖 周瑞宝 盖钧镒 《大豆科学》 CAS CSCD 北大核心 2009年第5期759-767,共9页
大豆种质蛋白质11S和7S及其亚基组相对含量的遗传变异是专用型品种选育的基础。以全国各生态区的野生豆138份和地方品种409份,国内育成品种148份、国外育成品种83份,合计778份大豆种质为材料,采用SDS-PAGE电泳技术测定蛋白质11S和7S组... 大豆种质蛋白质11S和7S及其亚基组相对含量的遗传变异是专用型品种选育的基础。以全国各生态区的野生豆138份和地方品种409份,国内育成品种148份、国外育成品种83份,合计778份大豆种质为材料,采用SDS-PAGE电泳技术测定蛋白质11S和7S组分及其亚基组相对含量,研究其遗传变异。在南京同一条件下的结果表明:全国野生豆、地方品种和育成品种11S相对含量平均分别为54.7%、64.8%和71.7%,变幅28.8%~82.6%、38.8%~79.4%和48.2%~88.9%;7S相对含量平均分别为44.7%、34.9%和27.9%,变幅20.6%~71.2%、20.6%~61.1%和15.7%~47.8%;11S/7S比值平均分别为1.4、2.0和2.7,变幅0.4~3.9、0.6~3.9和0.9~4.0。野生豆驯化为栽培豆并经选育后11S相对含量和11S/7S比值上升,7S相对含量下降,变幅均减小;亚基组11S-2和11S-3相对含量增加;7S的6个亚基组,尤其7S-1和7S-6,相对含量下降。11S、7S、11S/7S以及各亚基组在各群体各生态区内均有较大变异,但与来源地纬度、蛋白质和油脂含量均无显著相关。从中优选到11S/7S比值大于3.7、11S相对含量为78.9%~88.9%的8份种质,发现有11S的4个亚基组相对含量分别大于37%、7S的6个亚基组相对含量分别大于24%、以及11S-1和7S的6个亚基组缺失的种质,这些特异种质可供蛋白质组分育种利用。 展开更多
关键词 野生大豆(G.sojasieb.etZucc.) 栽培大豆(G.max(L.)Merr.) 11s蛋白质 7s蛋白质 11s/7s比值 蛋白质亚基组 遗传变异 生态区
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高产-S-腺苷蛋氨酸啤酒酵母诱变选育及其培养条件 被引量:5
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作者 汪文俊 肖靓 《武汉工业学院学报》 CAS 2007年第4期17-20,28,共5页
为了筛选高产S-腺-苷蛋氨酸(SAM)啤酒酵母突变株,采用不同剂量60Co射线对出发啤酒酵母菌悬液进行反复辐射处理,最终得到SAM高产突变株S-W55,并研究了其最优培养条件。结果表明最优化培养基为(%):葡萄糖4、L-蛋氨酸0.03、酵母浸粉0.4、Ca... 为了筛选高产S-腺-苷蛋氨酸(SAM)啤酒酵母突变株,采用不同剂量60Co射线对出发啤酒酵母菌悬液进行反复辐射处理,最终得到SAM高产突变株S-W55,并研究了其最优培养条件。结果表明最优化培养基为(%):葡萄糖4、L-蛋氨酸0.03、酵母浸粉0.4、CaCl20.01、KH2PO40.4、NaH2PO4 0.1、MgSO4 0.1,此条件下摇瓶发酵突变株S-W55的生物量和SAM产量分别达到12.15 g/L和1.88g/L,分别比未优化前提高了27.1%和19.7%。10 L发酵罐放大培养表明酵母突变株S-W55最适培养温度和pH值分别为28℃和5.0,发酵罐中其生物量、SAM产量比摇瓶发酵有了进一步的提高。 展开更多
关键词 ^60Co诱变 培养条件 s-腺苷-蛋氨酸 啤酒酵母
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